LOCUS       Exported                8332 bp ds-DNA     circular SYN 11-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        162..402
                     /label=U6 promoter
     misc_feature    382..406
                     /label=GIBSON HR
     misc_feature    407..410
                     /label=5' overhang
     misc_feature    411..430
                     /label=gRNA
     misc_feature    431..516
                     /label=Scaffold (F+E)
     misc_feature    431..450
                     /label=GIBSON HR
     misc_feature    431..434
                     /label=3'' overhang
     misc_feature    572..689
                     /label=cPPT/CTS
     promoter        828..2006
                     /label=EF-1-alpha promoter
     intron          1059..1997
                     /label=EF-1-alpha intron A
     CDS             2019..2618
                     /label=PuroR
     misc_feature    2634..3222
                     /label=WPRE
     CDS             complement(3105..3116)
                     /label=Factor Xa site
     LTR             3294..3527
                     /label=3' LTR (Delta-U3)
     polyA_signal    3605..3726
                     /label=SV40 poly(A) signal
     rep_origin      3766..3901
                     /label=SV40 ori
     promoter        complement(3922..3940)
                     /label=T7 promoter
     primer_bind     complement(3950..3966)
                     /label=M13 fwd
     rep_origin      4108..4563
                     /label=f1 ori
     promoter        4589..4693
                     /label=AmpR promoter
     CDS             4694..4762
                     /label=AmpR
     CDS             4763..5554
                     /label=AmpR
     rep_origin      5725..6313
                     /label=ori
     protein_bind    6601..6622
                     /label=CAP binding site
     promoter        6637..6642
                     /label=lac promoter
     promoter        6643..6660
                     /label=lac promoter
     promoter        6661..6667
                     /label=lac promoter
     protein_bind    6675..6691
                     /label=lac operator
     primer_bind     6699..6715
                     /label=M13 rev
     promoter        6736..6754
                     /label=T3 promoter
     promoter        6782..7008
                     /label=RSV promoter
     LTR             7009..7189
                     /label=5' LTR (truncated)
     misc_feature    7236..7361
                     /label=HIV-1 Psi
     misc_feature    7854..8087
                     /label=RRE
ORIGIN
        1 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
       61 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
      121 tatcgtttca gacccacctc ccaaccccga ggggacccag agagggccta tttcccatga
      181 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga
      241 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
      301 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
      361 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc ttggagccct
      421 ttgcagccta gtttaagagc tatgctggaa acagcatagc aagtttaaat aaggctagtc
      481 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttaagcttgg cgtaactaga
      541 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
      601 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
      661 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
      721 tttggcgccg gctcgagggg gcccgggtgc aaagatggat aaagttttaa acagagagga
      781 atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc
      841 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      901 attgatccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      961 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     1021 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
     1081 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
     1141 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
     1201 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
     1261 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
     1321 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
     1381 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
     1441 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
     1501 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
     1561 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     1621 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     1681 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     1741 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     1801 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     1861 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     1921 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     1981 tttttcttcc atttcaggtg tcgtgacgta cggccaccat gaccgagtac aagcccacgg
     2041 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     2101 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     2161 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     2221 acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     2281 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     2341 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     2401 tcggagtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     2461 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     2521 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     2581 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaac gcgttaagtc gacaatcaac
     2641 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     2701 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     2761 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     2821 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     2881 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     2941 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3001 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     3061 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     3121 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     3181 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat
     3241 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     3301 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     3361 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     3421 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     3481 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag
     3541 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     3601 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     3661 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     3721 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     3781 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     3841 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     3901 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     3961 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     4021 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     4081 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     4141 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4201 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4261 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4321 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4381 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4441 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4501 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     4561 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4621 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4681 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4741 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4801 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     4861 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     4921 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     4981 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5041 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5101 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5161 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5221 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5281 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5341 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5401 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5461 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5521 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5581 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5641 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5701 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5761 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5821 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5881 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5941 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6001 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6061 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6121 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6181 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6241 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6301 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6361 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6421 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     6481 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     6541 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     6601 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     6661 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     6721 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     6781 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     6841 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     6901 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     6961 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     7021 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7081 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7141 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     7201 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     7261 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     7321 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     7381 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     7441 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     7501 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     7561 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     7621 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     7681 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     7741 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     7801 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     7861 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     7921 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     7981 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     8041 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     8101 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     8161 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     8221 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     8281 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa ca
//
