LOCUS       Exported               14280 bp ds-DNA     circular SYN 20-MAY-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14280
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..636
                     /label=LTR
                     /note="label: LTR"
     misc_feature    1..453
                     /label=HIV U3
                     /note="label: HIV U3"
     repeat_region   454..551
                     /label=HIV R
                     /note="label: HIV\R"
     misc_feature    552..636
                     /label=HIV U5
                     /note="label: HIV U5"
     misc_feature    637..1155
                     /label=PSI
                     /note="label: PSI"
     misc_feature    697..806
                     /label=HIV-PSI
                     /note="label: HIV-PSI"
     misc_feature    1303..1536
                     /label=RRE
                     /note="label: RRE"
     misc_feature    2034..2211
                     /label=cppt
                     /note="label: cppt"
     misc_feature    2217..2222
                     /label=NheI
                     /note="label: NheI"
     promoter        2223..2810
                     /label=CMV
                     /note="label: CMV"
     primer_bind     2689..2709
                     /label=CMV-Forward
     TATA_signal     2724..2730
                     /label=TATA
                     /note="label: TATA"
     misc_binding    2740..2779
                     /label=TetO2
                     /note="label: TetO2"
     misc_feature    2882..2887
                     /label=XbaI
                     /note="label: XbaI"
     misc_feature    2908..2913
                     /label=XhoI
                     /note="label: XhoI"
     misc_recomb     2921..2928
                     /label=attR1
                     /note="label: attR1"
     primer_bind     2925..2944
                     /label=ATTB1
     misc_feature    2929..2945
                     /label=left
     misc_feature    2929..2940
                     /label=pENTR backbone
                     /note="label: pENTR backbone"
     LTR             2941..2965
                     /label=pENTR backbone
     CDS             2966..3661
                     /label=mScarlet-I
                     /note="codon_start: 1 note: matures faster than mScarlet 
                     product: bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016) 
                     transl_table: 1 translation: 
                     MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFSW
                     DILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGAVTVTQDTSLEDGT
                     LIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKMALRLKDGGRYLAD
                     FKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHSTGGMDELYK"
     misc_feature    3669..3672
                     /label=adaptor=GCCG
     CDS             3680..4372
                     /label=mScarlet-I
                     /note="codon_start: 1 note: matures faster than mScarlet 
                     product: bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016) 
                     transl_table: 1 translation: 
                     VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFSWD
                     ILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGAVTVTQDTSLEDGTL
                     IYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKMALRLKDGGRYLADF
                     KTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHSTGGMDELYK"
     misc_feature    4379..4382
                     /label=adaptor=ATCG
     CDS             4391..5083
                     /label=mScarlet-I
                     /note="codon_start: 1 note: matures faster than mScarlet 
                     product: bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016) 
                     transl_table: 1 translation: 
                     VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFSWD
                     ILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGGAVTVTQDTSLEDGTL
                     IYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKMALRLKDGGRYLADF
                     KTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHSTGGMDELYK"
     CDS             5084..5104
                     /label=LINKER
                     /note="codon_start: 1 transl_table: 1 translation: GGSGGGS"
     gene            5105..9205
                     /label=dCas9
                     /note="label: dCas9"
     primer_bind     complement(5334..5353)
                     /label=dCas9Seq1
     misc_feature    6000..6002
                     /label=potential silent mut
                     /note="label: potential silent mut"
     misc_feature    9206..9340
                     /label=HA-2XNLS
                     /note="label: HA-2XNLS"
     CDS             9209..9235
                     /label=HA tag
                     /note="label: HA tag"
     CDS             9254..9274
                     /label=NLS
                     /note="label: NLS"
     CDS             9281..9301
                     /label=NLS
                     /note="label: NLS"
     misc_feature    9344..9376
                     /label=pENTR backbone
                     /note="label: pENTR backbone"
     primer_bind     complement(9360..9383)
                     /label=ATTB2
     misc_recomb     9377..9384
                     /label=attR2
                     /note="label: attR2"
     promoter        9479..9983
                     /label=PGK
                     /note="label: PGK"
     misc_feature    9504..9526
                     /label=PHAGE PGK PCR3
                     /note="label: PHAGE PGK PCR3"
     gene            10004..10603
                     /label=Puro
                     /note="label: Puro"
     misc_feature    10669..11260
                     /label=WPRE
                     /note="label: WPRE"
     misc_feature    11331..11566
                     /label=dU3 LTR
                     /note="label: dU3\LTR"
     repeat_region   11368..11483
                     /label=HIV R
                     /note="label: HIV\R"
     misc_feature    11484..11566
                     /label=HIV U5
                     /note="label: HIV\U5"
     exon            11894..12733
                     /label=AMPr
                     /note="label: AMPr"
     rep_origin      12962..13495
                     /label=ORI
                     /note="label: ORI"
     rep_origin      13732..13855
                     /label=SV40 ORI
                     /note="label: SV40 ORI"
     primer_bind     13987..14006
                     /label=M13-48REV
     primer_bind     14004..14020
                     /label=M13R
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggcta
     2221 gcatgcatgt cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
     2761 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
     2821 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
     2881 ctctagagga tccctaccgg tgatatcctc gagcccatca acaagtttgt acaaaaaagc
     2941 aggctccgcg gccgccccct tcaccatggt gagcaagggc gaggcagtga tcaaggagtt
     3001 catgcggttc aaggtgcaca tggagggctc catgaacggc cacgagttcg agatcgaggg
     3061 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     3121 tggccccctg cccttctcct gggacatcct gtcccctcag ttcatgtacg gctccagggc
     3181 cttcatcaag caccccgccg acatccccga ctactataag cagtccttcc ccgagggctt
     3241 caagtgggag cgcgtgatga acttcgagga cggcggcgcc gtgaccgtga cccaggacac
     3301 ctccctggag gacggcaccc tgatctacaa ggtgaagctc cgcggcacca acttccctcc
     3361 tgacggcccc gtaatgcaga agaagacaat gggctgggaa gcgtccaccg agcggttgta
     3421 ccccgaggac ggcgtgctga agggcgacat taagatggcc ctgcgcctga aggacggcgg
     3481 ccgctacctg gcggacttca agaccaccta caaggccaag aagcccgtgc agatgcccgg
     3541 cgcctacaac gtcgaccgca agttggacat cacctcccac aacgaggact acaccgtggt
     3601 ggaacagtac gaacgctccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     3661 gtctggcggc cggtctggcg tgagcaaggg cgaggcagtg atcaaggagt tcatgcggtt
     3721 caaggtgcac atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga
     3781 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     3841 gcccttctcc tgggacatcc tgtcccctca gttcatgtac ggctccaggg ccttcatcaa
     3901 gcaccccgcc gacatccccg actactataa gcagtccttc cccgagggct tcaagtggga
     3961 gcgcgtgatg aacttcgagg acggcggcgc cgtgaccgtg acccaggaca cctccctgga
     4021 ggacggcacc ctgatctaca aggtgaagct ccgcggcacc aacttccctc ctgacggccc
     4081 cgtaatgcag aagaagacaa tgggctggga agcgtccacc gagcggttgt accccgagga
     4141 cggcgtgctg aagggcgaca ttaagatggc cctgcgcctg aaggacggcg gccgctacct
     4201 ggcggacttc aagaccacct acaaggccaa gaagcccgtg cagatgcccg gcgcctacaa
     4261 cgtcgaccgc aagttggaca tcacctccca caacgaggac tacaccgtgg tggaacagta
     4321 cgaacgctcc gagggccgcc actccaccgg cggcatggac gagctgtaca agggtagcat
     4381 cgacggtagc gtgagcaagg gcgaggcagt gatcaaggag ttcatgcggt tcaaggtgca
     4441 catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     4501 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttctc
     4561 ctgggacatc ctgtcccctc agttcatgta cggctccagg gccttcatca agcaccccgc
     4621 cgacatcccc gactactata agcagtcctt ccccgagggc ttcaagtggg agcgcgtgat
     4681 gaacttcgag gacggcggcg ccgtgaccgt gacccaggac acctccctgg aggacggcac
     4741 cctgatctac aaggtgaagc tccgcggcac caacttccct cctgacggcc ccgtaatgca
     4801 gaagaagaca atgggctggg aagcgtccac cgagcggttg taccccgagg acggcgtgct
     4861 gaagggcgac attaagatgg ccctgcgcct gaaggacggc ggccgctacc tggcggactt
     4921 caagaccacc tacaaggcca agaagcccgt gcagatgccc ggcgcctaca acgtcgaccg
     4981 caagttggac atcacctccc acaacgagga ctacaccgtg gtggaacagt acgaacgctc
     5041 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagggcggtt ccggcggagg
     5101 gtcggacaag aagtacagca tcggcctggc catcggcacc aactctgtgg gctgggccgt
     5161 gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg
     5221 gcacagcatc aagaagaacc tgatcggcgc cctgctgttc gacagcggag aaacagccga
     5281 ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg
     5341 ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag
     5401 actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg
     5461 caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa
     5521 gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca
     5581 catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga
     5641 cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc
     5701 catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag
     5761 acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggcaa
     5821 cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga
     5881 ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc
     5941 ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat
     6001 cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc
     6061 tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg
     6121 gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc
     6181 cggctacatc gatggcggag ccagccagga agagttctac aagttcatca agcccatcct
     6241 ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg
     6301 gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca
     6361 cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat
     6421 cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag
     6481 cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga
     6541 agtggtggac aagggcgcca gcgcccagag cttcatcgag cggatgacca acttcgataa
     6601 gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt
     6661 gtacaacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct
     6721 gagcggcgag cagaaaaaag ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac
     6781 cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat
     6841 ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat
     6901 tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt
     6961 gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc
     7021 ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg
     7081 caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct
     7141 ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga
     7201 cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct
     7261 gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac
     7321 agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt
     7381 gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag
     7441 aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc
     7501 cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg
     7561 ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg atgtggacgc
     7621 tatcgtgcct cagagctttc tgaaggacga ctccatcgat aacaaagtgc tgactcggag
     7681 cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa
     7741 gaactactgg cgccagctgc tgaatgccaa gctgattacc cagaggaagt tcgacaatct
     7801 gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca
     7861 gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa
     7921 cactaagtac gacgagaacg acaaactgat ccgggaagtg aaagtgatca ccctgaagtc
     7981 caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa
     8041 ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa
     8101 gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa
     8161 gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag
     8221 caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg
     8281 gcctctgatc gagacaaacg gcgaaacagg cgagatcgtg tgggataagg gccgggactt
     8341 tgccaccgtg cggaaagtgc tgtctatgcc ccaagtgaat atcgtgaaaa agaccgaggt
     8401 gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg acaagctgat
     8461 cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc
     8521 ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt
     8581 gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga
     8641 ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa
     8701 gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccggcgaact
     8761 gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag
     8821 ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga
     8881 acagcacaaa cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt
     8941 gatcctggcc gacgctaatc tggacaaggt gctgagcgcc tacaacaagc acagagacaa
     9001 gcctatcaga gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc
     9061 ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa
     9121 agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat
     9181 cgacctgtct cagctgggag gcgacgccta tccctatgac gtgcccgatt atgccagcct
     9241 gggcagcggc tcccccaaga aaaaacgcaa ggtggaagat cctaagaaaa agcggaaagt
     9301 ggacggcatt ggtagtggga gcaacggcag cagcggatcc tgaaagggtg ggcgcgccga
     9361 cccagctttc ttgtacaaag tggtttgcgc atagctaggt cgactagagg gcccgcggtt
     9421 cgaataccca tacgacgtcc cagactacgc ttagtaatga ttaattaaac tagaaattct
     9481 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     9541 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     9601 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     9661 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     9721 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     9781 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     9841 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     9901 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     9961 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc
    10021 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
    10081 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
    10141 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
    10201 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
    10261 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
    10321 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
    10381 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
    10441 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
    10501 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
    10561 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
    10621 gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg atagatccta atcaacctct
    10681 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
    10741 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
    10801 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
    10861 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
    10921 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
    10981 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
    11041 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
    11101 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
    11161 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
    11221 tcagacgagt cggatctccc tttgggccgc ctccccgcct gagatccttt aagaccaatg
    11281 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
    11341 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
    11401 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    11461 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    11521 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
    11581 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggccc
    11641 gggttaatta aggaaagggc tagatcattc ttgaagacga aagggcctcg tgatacgcct
    11701 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
    11761 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    11821 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
    11881 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    11941 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    12001 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    12061 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
    12121 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    12181 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    12241 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    12301 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    12361 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    12421 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    12481 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    12541 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    12601 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    12661 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    12721 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    12781 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    12841 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    12901 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    12961 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    13021 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
    13081 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    13141 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    13201 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    13261 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
    13321 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    13381 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    13441 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    13501 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
    13561 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
    13621 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
    13681 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcaagctcat
    13741 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
    13801 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccgtggca
    13861 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    13921 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
    13981 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga atttcacaaa
    14041 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    14101 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
    14161 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
    14221 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
