LOCUS       Exported               11974 bp ds-DNA     circular SYN 11-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..11974
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    395..983
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             991..1056
                     /label=nef (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=90.9"
                     /note="/match_length=22.7"
                     /note="/other=CDS"
     LTR             1058..1291
                     /label=3' LTR (Delta-U3)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     promoter        1496..1600
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1601..2461
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     rep_origin      2632..3220
                     /direction=RIGHT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             3403..3457
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     rep_origin      3463..3556
                     /direction=RIGHT
                     /label=SV40 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=69.1"
                     /note="/other=rep_origin"
     CDS             3581..3618
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.5"
                     /note="/other=CDS"
     protein_bind    3631..3652
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        3667..3697
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    3701..3723
                     /label=lac operator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=92.0"
                     /note="/other=protein_bind"
     polyA_signal    3755..3838
                     /label=SV40 poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=62.2"
                     /note="/other=polyA_signal"
     CDS             4005..4334
                     /label=nef (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=99.1"
                     /note="/match_length=53.4"
                     /note="/other=CDS"
     LTR             4382..4639
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    4686..4811
                     /label=HIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             4795..5133
                     /label=HIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=98.8"
                     /note="/match_length=22.6"
                     /note="/other=CDS"
     misc_feature    5308..5541
                     /label=RRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             5918..6018
                     /label=Rev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
     misc_feature    6063..6180
                     /label=cPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     enhancer        6237..6540
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        6541..6744
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        6746..6765
                     /label=PLtetO-1 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=27.0"
                     /note="/other=promoter"
     promoter        6767..6786
                     /label=PLtetO-1 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=27.0"
                     /note="/other=promoter"
     promoter        6794..6827
                     /label=TRE3GV promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=9.7"
                     /note="/other=promoter"
     protein_bind    6927..6951
                     /label=attB1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             6972..11243
                     /codon_start=1
                     /label=dCas9
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKMAPKKKRKVGIHGVPAADKKYS
                     IGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLK
                     RTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVD
                     EVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDK
                     LFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLI
                     ALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAIL
                     LSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYA
                     GYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGEL
                     HAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNF
                     EEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKP
                     AFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHD
                     LLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRR
                     YTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVS
                     GQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKG
                     QKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINR
                     LSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKL
                     ITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLI
                     REVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFV
                     YGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGE
                     TGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWD
                     PKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKG
                     YKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKL
                     KGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIRE
                     QAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLS
                     QLGGDKRPAATKKAGQAKKKK"
     misc_feature    6975..7040
                     /label=3xFLAG
     misc_signal     7047..7067
                     /label=SV40-NLS
     CDS             7092..11192
                     /label=dCas9
                     /label=dCas9(N)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     misc_feature    7117
                     /label=CtoA D10A
     misc_feature    9606..9607
                     /label=CAtoGC H840A
     CDS             11193..11240
                     /label=nucleoplasmin NLS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     protein_bind    11260..11284
                     /label=attB2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        11384..11883
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             join(11904..11974,1..328)
                     /label=BSD
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
ORIGIN
        1 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
       61 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac
      121 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
      181 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat
      241 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
      301 gccctctggt tatgtgtggg agggctaacg cccgccccac gacccgcagc gcccgaccga
      361 aaggagcgca cgaccccatg catcgataga tcctaatcaa cctctggatt acaaaatttg
      421 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
      481 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
      541 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
      601 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
      661 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
      721 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
      781 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
      841 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
      901 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
      961 ctccctttgg gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct
     1021 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     1081 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     1141 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     1201 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     1261 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     1321 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa
     1381 agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     1441 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     1501 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1561 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     1621 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     1681 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     1741 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     1801 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga
     1861 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     1921 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     1981 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     2041 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     2101 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     2161 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     2221 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     2281 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     2341 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     2401 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     2461 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2521 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2581 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2641 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2701 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2761 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     2821 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     2881 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2941 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3001 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3061 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3121 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3181 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3241 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3301 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     3361 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     3421 gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt
     3481 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     3541 ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga
     3601 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     3661 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     3721 atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc
     3781 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa
     3841 ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac
     3901 tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
     3961 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc
     4021 actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     4081 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     4141 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     4201 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     4261 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     4321 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     4381 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     4441 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     4501 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     4561 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     4621 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     4681 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     4741 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     4801 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     4861 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     4921 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     4981 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     5041 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     5101 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct
     5161 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     5221 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     5281 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     5341 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     5401 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     5461 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     5521 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     5581 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     5641 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     5701 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     5761 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     5821 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     5881 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     5941 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     6001 tcgattagtg aacggatctc gacggtatcg ccgaattcac aaatggcagt attcatccac
     6061 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     6121 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     6181 cgggtttatt acagggacag cagagatcca gtttggacta ggctagccat gcatgtcgtt
     6241 acataactta cggtaaatgg cccgcctggc tgaccgcccc acgacccccg cccattgacg
     6301 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     6361 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     6421 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     6481 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     6541 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     6601 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     6661 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     6721 tgggaggtct atataagcag agctctccct atcagtgata gagatctccc tatcagtgat
     6781 agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     6841 tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct agaggatccc
     6901 taccggtgat atcctcgagc ccatcaacaa gtttgtacaa aaaagcaggc tccgcggccg
     6961 cccccttcac catggactat aaggaccacg acggagacta caaggatcat gatattgatt
     7021 acaaagacga tgacgataag atggccccaa agaagaagcg gaaggtcggt atccacggag
     7081 tcccagcagc cgacaagaag tacagcatcg gcctggccat cggcaccaac tctgtgggct
     7141 gggccgtgat caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca
     7201 ccgaccggca cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa
     7261 cagccgaggc cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc
     7321 ggatctgcta tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct
     7381 tccacagact ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca
     7441 tcttcggcaa catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc
     7501 tgagaaagaa actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc
     7561 tggcccacat gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca
     7621 acagcgacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg
     7681 aaaaccccat caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca
     7741 agagcagacg gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt
     7801 tcggaaacct gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc
     7861 tggccgagga tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc
     7921 tgctggccca gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg
     7981 acgccatcct gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga
     8041 gcgcctctat gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc
     8101 tcgtgcggca gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg
     8161 gctacgccgg ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc
     8221 ccatcctgga aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc
     8281 tgctgcggaa gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag
     8341 agctgcacgc cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg
     8401 aaaagatcga gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg
     8461 gaaacagcag attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact
     8521 tcgaggaagt ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact
     8581 tcgataagaa cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact
     8641 tcaccgtgta taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg
     8701 ccttcctgag cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga
     8761 aagtgaccgt gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg
     8821 tggaaatctc cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc
     8881 tgaaaattat caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag
     8941 atatcgtgct gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa
     9001 cctatgccca cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg
     9061 gctggggcag gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga
     9121 caatcctgga tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc
     9181 acgacgacag cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg
     9241 atagcctgca cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc
     9301 tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga
     9361 acatcgtgat cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc
     9421 gcgagagaat gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag
     9481 aacaccccgt ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga
     9541 atgggcggga tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg
     9601 tggacgctat cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga
     9661 ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga
     9721 agatgaagaa ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg
     9781 acaatctgac caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca
     9841 agagacagct ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc
     9901 ggatgaacac taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc
     9961 tgaagtccaa gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga
    10021 tcaacaacta ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga
    10081 tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg
    10141 tgcggaagat gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct
    10201 tctacagcaa catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc
    10261 ggaagcggcc tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc
    10321 gggattttgc caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga
    10381 ccgaggtgca gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata
    10441 agctgatcgc cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca
    10501 ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga
    10561 agagtgtgaa agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc
    10621 ccatcgactt tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc
    10681 tgcctaagta ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg
    10741 gcgaactgca gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc
    10801 tggccagcca ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt
    10861 ttgtggaaca gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca
    10921 agagagtgat cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc
    10981 gggataagcc catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc
    11041 tgggagcccc tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca
    11101 gcaccaaaga ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga
    11161 cacggatcga cctgtctcag ctgggaggcg acaaaaggcc ggcggccacg aaaaaggccg
    11221 gccaggcaaa aaagaaaaag taaaagggtg ggcgcgccga cccagctttc ttgtacaaag
    11281 tggtttgcgc atagctaggt agcctagagg gcccgcggtt cgaataccca tacgacgtcc
    11341 cagactacgc ttagtaatga ttaattaaac tagaaattct accgggtagg ggaggcgctt
    11401 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
    11461 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
    11521 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
    11581 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
    11641 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
    11701 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
    11761 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
    11821 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
    11881 tcgacctgca gcccaagctt accatggcca agcctttgtc tcaagaagaa tccaccctca
    11941 ttgaaagagc aacggctaca atcaacagca tccc
//
