LOCUS       Exported                5561 bp ds-DNA     circular SYN 22-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Assmann Group
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 22, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        183..211
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    219..235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             258..1487
                     /codon_start=1
                     /label=StrepII-GNAI1
                     /translation="MSASSAWSHPQFEKSGGSGTSGGSAWSHPQFEKVGSLVPRGSAGE
                     NLYFQGGSGSMGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTI
                     VKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLF
                     VLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNY
                     IPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVA
                     LSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTI
                     CYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVII
                     KNNLKDCGLF"
     CDS             258..260
                     /codon_start=1
                     /label=ATG
                     /translation="M"
     CDS             276..299
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             333..356
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             366..383
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             390..410
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     gene            422..1487
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /label=GNAI1
                     /note="G protein subunit alpha i1"
                     /db_xref="GeneID:2770"
                     /db_xref="HGNC:HGNC:4384"
                     /db_xref="MIM:139310"
     exon            422..540
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /inference="alignment:same species:2.1.0"
     CDS             423..1487
                     /codon_start=1
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /product="guanine nucleotide-binding protein G(i) subunit
                     alpha-1 isoform 1"
                     /label=GNAI1
                     /note="isoform 1 is encoded by transcript variant 1; Gi1
                     protein alpha subunit; guanine nucleotide-binding protein 
                     G(i) subunit alpha-1; adenylate cyclase-inhibiting G alpha 
                     protein; guanine nucleotide binding protein (G protein), 
                     alpha inhibiting activity polypeptide 1; heterotrimeric 
                     guanine nucleotide-binding protein 1B"
                     /db_xref="CCDS:CCDS5595.1"
                     /db_xref="GeneID:2770"
                     /db_xref="HGNC:HGNC:4384"
                     /db_xref="MIM:139310"
                     /protein_id="NP_002060.4"
                     /translation="MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESG
                     KSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDA
                     RQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIA
                     QPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAII
                     FCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKS
                     PLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVT
                     DVIIKNNLKDCGLF"
     misc_feature    522..1466
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="Alpha subunit of G proteins (guanine nucleotide
                     binding); Region: G-alpha; cd00066"
                     /db_xref="CDD:206639"
     misc_feature    525..566
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="propagated from UniProtKB/Swiss-Prot (P63096.2);
                     Region: G1 motif. 
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU01230"
     misc_feature    540..563
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="G1 box; other site"
                     /db_xref="CDD:206639"
     exon            541..583
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    546..566
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="GTP/Mg2+ binding site [chemical binding]; other
                     site"
                     /db_xref="CDD:206639"
     exon            584..725
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /inference="alignment:same species:2.1.0"
     exon            726..883
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /inference="alignment:same species:2.1.0"
     exon            884..1012
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    939..965
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="propagated from UniProtKB/Swiss-Prot (P63096.2);
                     Region: G2 motif. 
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU01230"
     misc_feature    963..965
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="G2 box; other site"
                     /db_xref="CDD:206639"
     misc_feature    975..983
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="Switch I region; other site"
                     /db_xref="CDD:206639"
     misc_feature    1008..1037
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="propagated from UniProtKB/Swiss-Prot (P63096.2);
                     Region: G3 motif. 
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU01230"
     exon            1013..1142
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1020..1031
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="G3 box; other site"
                     /db_xref="CDD:206639"
     misc_feature    1026..1031
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="Switch II region; other site"
                     /db_xref="CDD:206639"
     misc_feature    1029..1031
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="GTP/Mg2+ binding site [chemical binding]; other
                     site"
                     /db_xref="CDD:206639"
     misc_feature    1077..1082
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="Switch II region; other site"
                     /db_xref="CDD:206639"
     exon            1143..1296
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1215..1238
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="propagated from UniProtKB/Swiss-Prot (P63096.2);
                     Region: G4 motif. 
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU01230"
     misc_feature    1227..1238
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="G4 box; other site"
                     /db_xref="CDD:206639"
     misc_feature    1227..1232
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="GTP/Mg2+ binding site [chemical binding]; other
                     site"
                     /db_xref="CDD:206639"
     misc_feature    1236..1238
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="GTP/Mg2+ binding site [chemical binding]; other
                     site"
                     /db_xref="CDD:206639"
     exon            1297..1487
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1392..1409
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="propagated from UniProtKB/Swiss-Prot (P63096.2);
                     Region: G5 motif. 
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU01230"
     misc_feature    1395..1403
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="GTP/Mg2+ binding site [chemical binding]; other
                     site"
                     /db_xref="CDD:206639"
     misc_feature    1395..1403
                     /gene="GNAI1"
                     /gene_synonym="Gi; HG1B"
                     /note="G5 box; other site"
                     /db_xref="CDD:206639"
     CDS             1509..1532
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        1864..1968
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1969..2829
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3000..3588
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3832..3909
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3910..4992
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     protein_bind    5005..5026
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5041..5071
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5079..5095
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5103..5119
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5115..5561
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGI"
     primer_bind     complement(5135..5151)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg agcgctagca gcgcgtggag ccacccgcag ttcgaaaaga
      301 gcggtggctc aggtaccagt ggcggttcag cctggagcca tccgcaattc gagaaggtcg
      361 gcagcctggt tccgcgtggt tctgctggtg aaaacctgta cttccagggt ggttcaggat
      421 ccatgggctg cacgctgagc gccgaggaca aggcggcggt ggagcggagt aagatgatcg
      481 accgcaacct ccgtgaggac ggcgagaagg cggcgcgcga ggtcaagctg ctgctgctcg
      541 gtgctggtga atctggtaaa agtacaattg tgaagcagat gaaaattatc catgaagctg
      601 gttattcaga agaggagtgt aaacaataca aagcagtggt ctacagtaac accatccagt
      661 caattattgc tatcattagg gctatgggga ggttgaagat agactttggt gactcagccc
      721 gggcggatga tgcacgccaa ctctttgtgc tagctggagc tgctgaagaa ggctttatga
      781 ctgcagaact tgctggagtt ataaagagat tgtggaaaga tagtggtgta caagcctgtt
      841 tcaacagatc ccgagagtac cagcttaatg attctgcagc atactatttg aatgacttgg
      901 acagaatagc tcaaccaaat tacatcccga ctcaacaaga tgttctcaga actagagtga
      961 aaactacagg aattgttgaa acccatttta ctttcaaaga tcttcatttt aaaatgtttg
     1021 atgtgggagg tcagagatct gagcggaaga agtggattca ttgcttcgaa ggagtgacgg
     1081 cgatcatctt ctgtgtagca ctgagtgact acgacctggt tctagctgaa gatgaagaaa
     1141 tgaaccgaat gcatgaaagc atgaaattgt ttgacagcat atgtaacaac aagtggttta
     1201 cagatacatc cattatactt tttctaaaca agaaggatct ctttgaagaa aaaatcaaaa
     1261 agagccctct cactatatgc tatccagaat atgcaggatc aaacacatat gaagaggcag
     1321 ctgcatatat tcaatgtcag tttgaagacc tcaataaaag aaaggacaca aaggaaatat
     1381 acacccactt cacatgtgcc acagatacta agaatgtgca gtttgttttt gatgctgtaa
     1441 cagatgtcat cataaaaaat aatctaaaag attgtggtct cttttaatcc ggagcgagca
     1501 ccggtgcaga ttataaggat gatgatgata agtaactgag taagcgatcg cagaattcat
     1561 cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     1621 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     1681 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     1741 agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     1801 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     1861 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1921 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     1981 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     2041 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     2101 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     2161 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     2221 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     2281 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2341 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2401 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2461 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     2521 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2581 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2641 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2701 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2761 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2821 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2881 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2941 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3001 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3061 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3121 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     3181 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3241 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3301 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3361 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3421 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3481 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3541 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3601 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3661 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3721 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     3781 cggtattttc tccttacgca tctgtgcggt atttcacacc gcataaattc cgacaccatc
     3841 gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg
     3901 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
     3961 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
     4021 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
     4081 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
     4141 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
     4201 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
     4261 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
     4321 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
     4381 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
     4441 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
     4501 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
     4561 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
     4621 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
     4681 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
     4741 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
     4801 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
     4861 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     4921 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     4981 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     5041 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     5101 cacaggaaac agctatgacc atgattacgg attcactggc cgtcgtttta caacgtcgtg
     5161 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     5221 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     5281 atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg
     5341 agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt
     5401 acgatgcgcc catctacacc aacgtaacct atcccattac ggtcaatccg ccgtttgttc
     5461 ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac
     5521 aggaaggcca gacgcgaatt atttttgatg gcgttggaat t
//
