LOCUS       Exported                5570 bp ds-DNA     circular SYN 22-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Assmann Group
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Oct 22, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        183..211
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    219..235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             258..1490
                     /codon_start=1
                     /label=StrepII-GNAO1
                     /translation="MSASSAWSHPQFEKSGGSGTSGGSAWSHPQFEKVGSLVPRGSAGE
                     NLYFQGGSGSMMGCTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGAGESGKST
                     IVKQMKIIHEDGFSGEDVKQYKPVVYSNTIQSLAAIVRAMDTLGIEYGDKERKADAKMV
                     CDVVSRMEDTEPFSAELLSAMMRLWGDSGIQECFNRSREYQLNDSAKYYLDSLDRIGAA
                     DYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIFC
                     VALSGYDQVLHEDETTNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKKSPL
                     TICFPEYTGPNTYEDAAAYIQAQFESKNRSPNKEIYCHMTCATDTNNIQVVFDAVTDII
                     IANNLRGCGLY"
     CDS             258..260
                     /codon_start=1
                     /label=ATG
                     /translation="M"
     CDS             276..299
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             333..356
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             366..383
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             390..410
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     gene            426..1490
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /label=GNAO1
                     /note="guanine nucleotide binding protein (G protein), 
                     alpha activating activity polypeptide O"
                     /db_xref="GeneID:2775"
                     /db_xref="HGNC:HGNC:4389"
                     /db_xref="HPRD:00757"
                     /db_xref="MIM:139311"
     CDS             426..1490
                     /codon_start=1
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /product="guanine nucleotide-binding protein G(o) subunit 
                     alpha isoform a"
                     /label=GNAO1
                     /note="isoform a is encoded by transcript variant 1; 
                     guanine nucleotide-binding protein G(o) subunit alpha"
                     /db_xref="GI:10567816"
                     /db_xref="CCDS:CCDS10756.1"
                     /db_xref="GeneID:2775"
                     /db_xref="HGNC:HGNC:4389"
                     /db_xref="HPRD:00757"
                     /db_xref="MIM:139311"
                     /protein_id="NP_066268.1"
                     /translation="MGCTLSAEERAALERSKAIEKNLKEDGISAAKDVKLLLLGAGESG
                     KSTIVKQMKIIHEDGFSGEDVKQYKPVVYSNTIQSLAAIVRAMDTLGIEYGDKERKADA
                     KMVCDVVSRMEDTEPFSAELLSAMMRLWGDSGIQECFNRSREYQLNDSAKYYLDSLDRI
                     GAADYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAI
                     IFCVALSGYDQVLHEDETTNRMHESLMLFDSICNNKFFIDTSIILFLNKKDLFGEKIKK
                     SPLTICFPEYTGPNTYEDAAAYIQAQFESKNRSPNKEIYCHMTCATDTNNIQVVFDAVT
                     DIIIANNLRGCGLY"
     exon            426..543
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /inference="alignment:same species:1.39.8"
     misc_feature    465..1481
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="G protein alpha subunit; Region: G_alpha; 
                     smart00275"
                     /db_xref="CDD:214595"
     misc_feature    525..1469
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="Alpha subunit of G proteins (guanine nucleotide 
                     binding); Region: G-alpha; cd00066"
                     /db_xref="CDD:206639"
     misc_feature    543..566
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /label=G1
                     /note="G1 box; other site"
                     /db_xref="CDD:206639"
     misc_feature    543..545
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GTP/Mg2+ binding site [chemical binding]; other 
                     site"
                     /db_xref="CDD:206639"
     exon            544..586
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /inference="alignment:same species:1.39.8"
     misc_feature    546..554
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GoLoco binding site; other site"
                     /db_xref="CDD:206639"
     misc_feature    558..566
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GTP/Mg2+ binding site [chemical binding]; other 
                     site"
                     /db_xref="CDD:206639"
     exon            587..728
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /inference="alignment:same species:1.39.8"
     misc_feature    636..638
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GoLoco binding site; other site"
                     /db_xref="CDD:206639"
     misc_feature    648..650
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GoLoco binding site; other site"
                     /db_xref="CDD:206639"
     misc_feature    660..662
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GoLoco binding site; other site"
                     /db_xref="CDD:206639"
     misc_feature    669..671
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GoLoco binding site; other site"
                     /db_xref="CDD:206639"
     misc_feature    678..683
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GoLoco binding site; other site"
                     /db_xref="CDD:206639"
     exon            729..889
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /inference="alignment:same species:1.39.8"
     misc_feature    735..737
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GoLoco binding site; other site"
                     /db_xref="CDD:206639"
     misc_feature    744..746
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GoLoco binding site; other site"
                     /db_xref="CDD:206639"
     misc_feature    864..866
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="Phosphotyrosine. {ECO:0000250|UniProtKB:P11488}; 
                     propagated from UniProtKB/Swiss-Prot (P09471.4); 
                     phosphorylation site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    873..875
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GoLoco binding site; other site"
                     /db_xref="CDD:206639"
     exon            890..1018
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /inference="alignment:same species:1.39.8"
     STS             896..1126
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /standard_name="PMC19731P1"
                     /label=PMC19731P1
                     /db_xref="UniSTS:271857"
     misc_feature    954..980
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /label=Switch I
                     /note="Switch I region; other site"
                     /db_xref="CDD:206639"
     misc_feature    960..962
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="ADP-ribosylarginine, by cholera toxin. 
                     {ECO:0000250}; propagated from UniProtKB/Swiss-Prot 
                     (P09471.4); modified site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     misc_feature    960..962
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GoLoco binding site; other site"
                     /db_xref="CDD:206639"
     misc_feature    969..971
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GTP/Mg2+ binding site [chemical binding]; other 
                     site"
                     /db_xref="CDD:206639"
     misc_feature    969..971
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /label=G2
                     /note="G2 box; other site"
                     /db_xref="CDD:206639"
     misc_feature    972..974
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="beta - gamma complex interaction site [polypeptide 
                     binding]; other site"
                     /db_xref="CDD:206639"
     misc_feature    978..980
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="adenylyl cyclase interaction site [polypeptide 
                     binding]; other site"
                     /db_xref="CDD:206639"
     misc_feature    978..980
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="beta - gamma complex interaction site [polypeptide 
                     binding]; other site"
                     /db_xref="CDD:206639"
     misc_feature    984..986
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="beta - gamma complex interaction site [polypeptide 
                     binding]; other site"
                     /db_xref="CDD:206639"
     misc_feature    1017..1019
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="beta - gamma complex interaction site [polypeptide 
                     binding]; other site"
                     /db_xref="CDD:206639"
     exon            1019..1148
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /inference="alignment:same species:1.39.8"
     misc_feature    1023..1025
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="beta - gamma complex interaction site [polypeptide 
                     binding]; other site"
                     /db_xref="CDD:206639"
     misc_feature    1026..1037
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /label=G3
                     /note="G3 box; other site"
                     /db_xref="CDD:206639"
     misc_feature    1026..1028
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GTP/Mg2+ binding site [chemical binding]; other 
                     site"
                     /db_xref="CDD:206639"
     misc_feature    1029..1079
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /label=Switch II
                     /note="Switch II region; other site"
                     /db_xref="CDD:206639"
     misc_feature    1035..1043
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="beta - gamma complex interaction site [polypeptide 
                     binding]; other site"
                     /db_xref="CDD:206639"
     misc_feature    1035..1037
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GTP/Mg2+ binding site [chemical binding]; other 
                     site"
                     /db_xref="CDD:206639"
     misc_feature    1035..1037
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GoLoco binding site; other site"
                     /db_xref="CDD:206639"
     misc_feature    1047..1049
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="beta - gamma complex interaction site [polypeptide 
                     binding]; other site"
                     /db_xref="CDD:206639"
     misc_feature    1053..1055
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="adenylyl cyclase interaction site [polypeptide 
                     binding]; other site"
                     /db_xref="CDD:206639"
     misc_feature    1056..1061
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="beta - gamma complex interaction site [polypeptide 
                     binding]; other site"
                     /db_xref="CDD:206639"
     misc_feature    1059..1061
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GoLoco binding site; other site"
                     /db_xref="CDD:206639"
     misc_feature    1062..1067
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="adenylyl cyclase interaction site [polypeptide 
                     binding]; other site"
                     /db_xref="CDD:206639"
     misc_feature    1065..1076
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="beta - gamma complex interaction site [polypeptide 
                     binding]; other site"
                     /db_xref="CDD:206639"
     misc_feature    1071..1073
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GoLoco binding site; other site"
                     /db_xref="CDD:206639"
     misc_feature    1074..1076
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="adenylyl cyclase interaction site [polypeptide 
                     binding]; other site"
                     /db_xref="CDD:206639"
     exon            1149..1302
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /inference="alignment:same species:1.39.8"
     misc_feature    1233..1244
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /label=G4
                     /note="G4 box; other site"
                     /db_xref="CDD:206639"
     misc_feature    1233..1238
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GTP/Mg2+ binding site [chemical binding]; other 
                     site"
                     /db_xref="CDD:206639"
     misc_feature    1242..1244
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GTP/Mg2+ binding site [chemical binding]; other 
                     site"
                     /db_xref="CDD:206639"
     exon            1303..1490
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /inference="alignment:same species:1.39.8"
     misc_feature    1374..1409
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="putative receptor binding site; other site"
                     /db_xref="CDD:206639"
     misc_feature    1398..1406
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /label=G5
                     /note="G5 box; other site"
                     /db_xref="CDD:206639"
     misc_feature    1398..1403
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="GTP/Mg2+ binding site [chemical binding]; other 
                     site"
                     /db_xref="CDD:206639"
     misc_feature    1413..1424
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="putative receptor binding site; other site"
                     /db_xref="CDD:206639"
     misc_feature    1476..1478
                     /gene="GNAO1"
                     /gene_synonym="EIEE17; G-ALPHA-o; GNAO"
                     /note="ADP-ribosylcysteine, by pertussis toxin. 
                     {ECO:0000250}; propagated from UniProtKB/Swiss-Prot 
                     (P09471.4); modified site"
                     /experiment="experimental evidence, no additional details 
                     recorded"
     CDS             1518..1541
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        1873..1977
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1978..2838
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3009..3597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3841..3918
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3919..5001
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     protein_bind    5014..5035
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5050..5080
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5088..5104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5112..5128
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5124..5570
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGI"
     primer_bind     complement(5144..5160)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg agcgctagca gcgcgtggag ccacccgcag ttcgaaaaga
      301 gcggtggctc aggtaccagt ggcggttcag cctggagcca tccgcaattc gagaaggtcg
      361 gcagcctggt tccgcgtggt tctgctggtg aaaacctgta cttccagggt ggttcaggat
      421 ccatgatggg atgtactctg agcgcagagg agagagccgc cctcgagcgg agcaaggcga
      481 ttgagaaaaa cctcaaagag gatggcatca gcgccgccaa agacgtgaaa ttactcctgc
      541 tcggggctgg agaatcagga aaaagcacca ttgtgaagca gatgaagatc atccatgaag
      601 atggcttctc cggagaagac gtgaaacagt acaagcctgt tgtctacagc aacactatcc
      661 agtccctggc agccatcgtc cgggccatgg acactttggg catcgaatat ggtgataagg
      721 agagaaaggc tgacgccaag atggtgtgtg atgtggtgag tcggatggaa gacaccgagc
      781 ccttctctgc agagctgctt tctgccatga tgcggctctg gggcgactca ggaatccaag
      841 agtgcttcaa ccggtcccgg gagtatcagc tcaacgactc tgccaaatac tacctggaca
      901 gcctggatcg gattggggcc gccgactacc agcccaccga gcaggacatc ctccgaacca
      961 gggtcaaaac cactggcatc gtagaaaccc acttcacatt caagaacctc cacttcaggc
     1021 tgtttgacgt cggaggccag cgatctgaac gcaagaagtg gatccattgc ttcgaggacg
     1081 tcacggccat cattttctgt gtcgcgctca gcggctatga ccaggtgctc cacgaagacg
     1141 aaaccacgaa ccgcatgcac gagtctctca tgctcttcga ctccatctgt aacaacaagt
     1201 tcttcatcga tacctccatc attctcttcc tcaacaagaa agatctcttt ggcgagaaga
     1261 tcaagaagtc acctttgacc atctgctttc ctgaatacac aggccccaat acctatgaag
     1321 acgcagccgc ctacatccaa gcacaatttg aaagcaaaaa ccgctcaccc aacaaagaaa
     1381 tatattgtca catgacttgt gccacagaca cgaataacat ccaggtggtg ttcgacgccg
     1441 tcaccgacat catcattgcc aacaacctcc ggggctgcgg cttgtactga gtgacgtccg
     1501 gagcgagcac cggtgcagat tataaggatg atgatgataa gtaactgagt aagcgatcgc
     1561 agaattcatc gtgactgact gacgatctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     1621 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     1681 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     1741 cagtcacgta gcgatagcgg agtgtataat tcttgaagac gaaagggcct cgtgatacgc
     1801 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     1861 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     1921 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1981 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2041 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2101 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2161 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2221 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2281 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2341 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2401 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2461 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2521 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2581 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2641 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     2701 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2761 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2821 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     2881 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2941 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3001 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3061 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3121 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     3181 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3241 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3301 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3361 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3421 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3481 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3541 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3601 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3661 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3721 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3781 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataaattcc
     3841 gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt
     3901 caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt
     3961 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
     4021 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
     4081 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
     4141 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
     4201 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
     4261 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
     4321 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
     4381 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg
     4441 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
     4501 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
     4561 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
     4621 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
     4681 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
     4741 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
     4801 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
     4861 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
     4921 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     4981 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     5041 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     5101 acaatttcac acaggaaaca gctatgacca tgattacgga ttcactggcc gtcgttttac
     5161 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     5221 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     5281 gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa
     5341 gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga
     5401 tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc
     5461 cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa
     5521 gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttggaatt
//
