LOCUS       5._pAAV_CAG_del_-FLEx_loxP_-mCherry_nuclear_-2A-N2cG 7507 bp    DNA     circular SYN 17-JAN-2026
DEFINITION  5._pAAV_CAG_del_-FLEx_loxP_-mCherry_nuclear_-2A-N2cG.
ACCESSION   5._pAAV_CAG_del_-FLEx_loxP_-mCherry_nuclear_-2A-N2cG
VERSION     5._pAAV_CAG_del_-FLEx_loxP_-mCherry_nuclear_-2A-N2cG
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     misc_feature    complement(1281..1869)
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
                     /description="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     rep_origin      5891..6319
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /description="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis "
     CDS             2168..2872
                     /note="pLannotate"
                     /label="mCherry"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
                     /description=""
     promoter        complement(5102..5379)
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="No description available"
     CDS             6477..7337
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="β-lactamase; bla; confers resistance to
                     ampicillin, carbenicillin, and related antibiotics"
     CDS             2945..4516
                     /note="pLannotate"
                     /label="GLYCO_RABVR"
                     /database="swissprot"
                     /identity="99.4"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. Attaches the virus to host
                     cellular receptor, inducing endocytosis of the virion by
                     using different host proteins including TFRC, GRM2 and
                     ITGB1 (By similarity). In the endosome, the acidic pH
                     induces conformational changes in the glycoprotein trimer,
                     which trigger fusion between virus and cell membrane. There
                     is convincing in vitro evidence that the muscular form of
                     the nicotinic acetylcholine receptor (nAChR), the neuronal
                     cell adhesion molecule (NCAM), and the p75 neurotrophin
                     receptor (p75NTR) bind glycoprotein and thereby facilitate
                     rabies virus entry into cells (By similarity). From Rabies
                     virus (strain China/MRV) (RABV)."
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /description="high-copy-number ColE1/pMB1/pBR322/pUC origin
                     of replication "
     repeat_region   5690..5819
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="itrs"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="repeat_region"
                     /description="AAV2 ITR Flip"
     repeat_region   complement(826..955)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="itrs"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="repeat_region"
                     /description="AAV2 ITR Flip"
     polyA_signal    1117..1238
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /description="SV40 polyadenylation signal"
     promoter        6372..6476
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="bla"
     enhancer        complement(5381..5629)
                     /note="pLannotate"
                     /label="CMV enhancer (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="81.9"
                     /fragment="True"
                     /other="enhancer"
                     /description="human cytomegalovirus immediate early
                     enhancer"
     protein_bind    4556..4589
                     /note="pLannotate"
                     /label="loxP"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="Cre-mediated recombination occurs in the 8-bp
                     core sequence (GCATACAT). Cre recombinase"
     protein_bind    complement(1911..1944)
                     /note="pLannotate"
                     /label="loxP"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="Cre-mediated recombination occurs in the 8-bp
                     core sequence (GCATACAT). Cre recombinase"
     protein_bind    4652..4685
                     /note="pLannotate"
                     /label="lox2272"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="Cre-mediated recombination occurs in the 8-bp
                     core sequence (GGATACTT). lox2272 sites are compatible with
                     each other, but incompatible with loxP or loxN sites (Lee
                     and Saito, 1988). Cre recombinase"
     protein_bind    complement(2015..2048)
                     /note="pLannotate"
                     /label="lox2272"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="Cre-mediated recombination occurs in the 8-bp
                     core sequence (GGATACTT). lox2272 sites are compatible with
                     each other, but incompatible with loxP or loxN sites (Lee
                     and Saito, 1988). Cre recombinase"
     intron          complement(4966..5101)
                     /note="pLannotate"
                     /label="hybrid intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="59.6"
                     /fragment="True"
                     /other="intron"
                     /description="hybrid between chicken β-actin (CBA) and
                     minute virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             2081..2101
                     /note="pLannotate"
                     /label="SV40 NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
     CDS             2129..2167
                     /note="pLannotate"
                     /label="V5 tag (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="92.9"
                     /fragment="True"
                     /other="CDS"
                     /description="epitope tag from simian virus 5"
     CDS             5817..5886
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="40.2"
                     /fragment="True"
                     /other="CDS"
                     /description="LacZα fragment of β-galactosidase; lacZ
                     fragment"
     intron          complement(4747..4877)
                     /note="pLannotate"
                     /label="chimeric intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="12.9"
                     /fragment="True"
                     /other="intron"
                     /description="chimera between introns from chicken β-actin
                     and rabbit β-globin"
     misc_feature    complement(1869..1893)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="23.1"
                     /fragment="True"
                     /other="misc_feature"
                     /description="pBluescript multiple cloning site"
     CDS             complement(4684..4749)
                     /note="pLannotate"
                     /label="HBB_LEPEU (fragment)"
                     /database="swissprot"
                     /identity="81.8"
                     /match_length="15.0"
                     /fragment="True"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. Involved in oxygen transport
                     from the lung to the various peripheral tissues. From Lepus
                     europaeus (European hare)."
     repeat_region   complement(749..760)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="itrs"
                     /identity="100.0"
                     /match_length="8.3"
                     /fragment="True"
                     /other="repeat_region"
                     /description="AAV2 ITR Flip"
     CDS             772..828
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.3"
                     /fragment="True"
                     /other="CDS"
                     /description="lac repressor; lacI; The lac repressor binds
                     to the lac operator to inhibit transcription in E. coli.
                     This inhibition can be relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     CDS             1026..1094
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="98.6"
                     /match_length="3.2"
                     /fragment="True"
                     /other="CDS"
                     /description="large T antigen from SV40 (simian virus 40)"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg cgctcgctcg
      841 ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca
      901 gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttccttgtag
      961 ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa gatccttatc
     1021 gattttacca catttgtaga ggttttactt gctttaaaaa acctcccaca tctccccctg
     1081 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     1141 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     1201 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgctc gaagcggccg
     1261 cccgggtcga cggtatcgat gcggggaggc ggcccaaagg gagatccgac tcgtctgagg
     1321 gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc
     1381 cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc
     1441 aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca cggaattgtc
     1501 agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc
     1561 cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat
     1621 gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac
     1681 aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat
     1741 gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag
     1801 cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag
     1861 aggttgatta tcgataagct tgatatcgaa ttcgaacctc ttcgagggac ataacttcgt
     1921 ataatgtatg ctatacgaag ttatgggtcg atggtgagat ctggactaga gggtcgatgg
     1981 tgatgcttgg atcaaaatag gaagaatccc aatgataact tcgtataaag tatcctatac
     2041 gaagttatac tagaattcgg taccgctagc caccatggat ccaaaaaaga agagaaaggt
     2101 agatccaaag aaaaagcgca aggtagggaa gcctatccct aaccctctcc tcggtctcga
     2161 ttctacggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     2221 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     2281 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     2341 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     2401 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     2461 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     2521 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     2581 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     2641 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     2701 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     2761 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     2821 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agactagtgg
     2881 atccggagct actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg
     2941 acccatggtt cctcaggttc ttttgtttgt actccttctg ggtttttcgt tgtgtttcgg
     3001 gaagttcccc atttacacga taccagacga acttggtccc tggagcccta ttgacataca
     3061 ccatctcagc tgtccaaata acctggttgt ggaggatgaa ggatgtacca acctgtccga
     3121 gttctcctac atggaactca aagtgggata catctcagcc atcaaagtga acgggttcac
     3181 ttgcacaggt gttgtgacag aggcagagac ctacaccaac tttgttggtt atgtcacaac
     3241 cacattcaag agaaagcatt tccgccccac cccagacgca tgtagagccg cgtataactg
     3301 gaagatggcc ggtgacccca gatatgaaga gtccctacac aatccatacc ccgactacca
     3361 ctggcttcga actgtaagaa ccaccaaaga gtccctcatt atcatatccc caagtgtgac
     3421 agatttggac ccatatgaca aatcccttca ctcaagggtc ttccctggcg gaaagtgctc
     3481 aggaataacg gtgtcctcta cctactgctc aactaaccat gattacacca tttggatgcc
     3541 cgagaatccg agaccaagga caccttgtga catttttacc aatagcagag ggaagagagc
     3601 atccaacggg aacaagactt gcggctttgt ggatgaaaga ggcctgtata agtctctaaa
     3661 aggagcatgc aggctcaagt tatgtggagt tcttggactt agacttatgg atggaacatg
     3721 ggtcgcgatg caaacatcag atgagaccaa atggtgccct ccagatcagt tggtgaattt
     3781 gcacgacttt cgctcagacg agattgagca tctcgttgtg gaggagttag tcaagaaaag
     3841 agaggaatgt ctggatgcat tagagtccat catgaccacc aagtcagtaa gtttcagacg
     3901 tctcagtcac ctgagaaaac ttgtcccagg gtttggaaaa gcatatacca tattcaacaa
     3961 aaccttgatg gaggctgatg ctcactacaa gtcagtccgg acctggaatg agatcatccc
     4021 ctcaaaaggg tgtttgaaag ttggaggaag gtgccatcct catgtgaacg gggtgttttt
     4081 caatggtata atattagggc ctgacgacca tgtcctaatc ccagagatgc aatcatccct
     4141 cctccagcaa catatggagt tgttggaatc ttcagttatc cccctgatgc accccctggc
     4201 agacccttct acagttttca aagaaggtga tgaggctgag gattttgttg aagttcacct
     4261 ccccgatgtg tacaaacaga tctcaggggt tgacctgggt ctcccgaact ggggaaagta
     4321 tgtattgatg actgcagggg ccatgattgg cctggtgttg atattttccc taatgacatg
     4381 gtgcagaaga gccaatcgac cagaatcgaa acaacgcagt tttggaggga caggggggaa
     4441 tgtgtcagtc acttcccaaa gcggaaaagt cataccttca tgggaatcat ataagagtgg
     4501 aggtgagatc agactgtagg cgcgcctcga gtgtacaagt aaggggatcc ctagtataac
     4561 ttcgtatagc atacattata cgaagttatg ggtcgatggt gatgcttggc aattcgggtc
     4621 gatggtgaag cattggtctt cctattttga tataacttcg tataggatac tttatacgaa
     4681 gttatgggga tccaattctt tgccaaaatg atgagacagc acaataacca gcacgttgcc
     4741 caggagctgt aggaaaaaga agaaggcatg aacatggtta gcagaggctc tagagccgcc
     4801 ggtcacacgc cagaagccga accccgccct gccccgtccc ccccgaaggc agccgtcccc
     4861 ccgcggacag ccccgagccg ctccccccgc acaaagggcc ctcccggagc ccctcaaggc
     4921 tttcacgcag ccacagaaaa gaaacaagcc gtcattaaac caagcgctaa ttacagcccg
     4981 gaggagaagg gccgtcccgc ccgctcacct gtgggagtaa cgcggtcagt cagagccggg
     5041 gcgggcggcg cgaggcggcg gcggagcggg gcacggggcg aaggcagcgc gcagcgactc
     5101 ccgcccgccg cgcgcttcgc tttttatagg gccgccgccg ccgccgcctc gccataaaag
     5161 gaaactttcg gagcgcgccg ctctgattgg ctgccgccgc acctctccgc ctcgccccgc
     5221 cccgcccctc gccccgcccc gccccgcctg gcgcgcgccc cccccccccc cccgccccca
     5281 tcgctgcaca aaataattaa aaaataaata aatacaaaat tgggggtggg gagggggggg
     5341 agatggggag agtgaagcag aacgtggggc tcacctcgac catggtaata gcgatgacta
     5401 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
     5461 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
     5521 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
     5581 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggt actcttccta
     5641 gagcatggct acgtagataa gtagcatggc gggttaatca ttaactacaa ggaaccccta
     5701 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     5761 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     5821 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     5881 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5941 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6001 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6061 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6121 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     6181 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     6241 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     6301 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     6361 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6421 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6481 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6541 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6601 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6661 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6721 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6781 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6841 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6901 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6961 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7021 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7081 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7141 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7201 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7261 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7321 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7381 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7441 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7501 gatcttc
//
