LOCUS       1._pAAV_CAG_del_-FLEx_loxP_-ARch3.0_EYFP 6636 bp    DNA     circular SYN 17-JAN-2026
DEFINITION  1._pAAV_CAG_del_-FLEx_loxP_-ARch3.0_EYFP.
ACCESSION   1._pAAV_CAG_del_-FLEx_loxP_-ARch3.0_EYFP
VERSION     1._pAAV_CAG_del_-FLEx_loxP_-ARch3.0_EYFP
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     misc_feature    complement(1281..1869)
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
                     /description="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     rep_origin      5020..5448
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /description="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis "
     CDS             2909..3622
                     /note="pLannotate"
                     /label="EYFP"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="99.6"
                     /fragment="False"
                     /other="CDS"
                     /description=""
     CDS             5606..6466
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /description="β-lactamase; bla; confers resistance to
                     ampicillin, carbenicillin, and related antibiotics"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /description="high-copy-number ColE1/pMB1/pBR322/pUC origin
                     of replication "
     repeat_region   4819..4948
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="itrs"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="repeat_region"
                     /description="AAV2 ITR Flip"
     repeat_region   complement(826..955)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="itrs"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="repeat_region"
                     /description="AAV2 ITR Flip"
     polyA_signal    1117..1238
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
                     /description="SV40 polyadenylation signal"
     CDS             2075..2848
                     /note="pLannotate"
                     /label="BACR1_HALEZ"
                     /database="swissprot"
                     /identity="93.8"
                     /match_length="99.2"
                     /fragment="False"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. Light-driven proton pump. It may
                     interact with bacterioruberin in the claret membrane. From
                     Halorubrum ezzemoulense (Halorubrum chaoviator)."
     promoter        5501..5605
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /description="bla"
     promoter        complement(4230..4508)
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.4"
                     /fragment="False"
                     /other="promoter"
                     /description="No description available"
     enhancer        complement(4510..4758)
                     /note="pLannotate"
                     /label="CMV enhancer (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="81.9"
                     /fragment="True"
                     /other="enhancer"
                     /description="human cytomegalovirus immediate early
                     enhancer"
     protein_bind    3684..3717
                     /note="pLannotate"
                     /label="loxP"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="Cre-mediated recombination occurs in the 8-bp
                     core sequence (GCATACAT). Cre recombinase"
     protein_bind    complement(1911..1944)
                     /note="pLannotate"
                     /label="loxP"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="Cre-mediated recombination occurs in the 8-bp
                     core sequence (GCATACAT). Cre recombinase"
     protein_bind    3780..3813
                     /note="pLannotate"
                     /label="lox2272"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="Cre-mediated recombination occurs in the 8-bp
                     core sequence (GGATACTT). lox2272 sites are compatible with
                     each other, but incompatible with loxP or loxN sites (Lee
                     and Saito, 1988). Cre recombinase"
     protein_bind    complement(2015..2048)
                     /note="pLannotate"
                     /label="lox2272"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
                     /description="Cre-mediated recombination occurs in the 8-bp
                     core sequence (GGATACTT). lox2272 sites are compatible with
                     each other, but incompatible with loxP or loxN sites (Lee
                     and Saito, 1988). Cre recombinase"
     intron          complement(4094..4229)
                     /note="pLannotate"
                     /label="hybrid intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="59.6"
                     /fragment="True"
                     /other="intron"
                     /description="hybrid between chicken β-actin (CBA) and
                     minute virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             4946..5015
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="40.2"
                     /fragment="True"
                     /other="CDS"
                     /description="LacZα fragment of β-galactosidase; lacZ
                     fragment"
     intron          complement(3875..4005)
                     /note="pLannotate"
                     /label="chimeric intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="12.9"
                     /fragment="True"
                     /other="intron"
                     /description="chimera between introns from chicken β-actin
                     and rabbit β-globin"
     misc_feature    complement(1869..1893)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="23.1"
                     /fragment="True"
                     /other="misc_feature"
                     /description="pBluescript multiple cloning site"
     CDS             complement(3812..3877)
                     /note="pLannotate"
                     /label="HBB_LEPEU (fragment)"
                     /database="swissprot"
                     /identity="81.8"
                     /match_length="15.0"
                     /fragment="True"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. Involved in oxygen transport
                     from the lung to the various peripheral tissues. From Lepus
                     europaeus (European hare)."
     repeat_region   complement(749..760)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="itrs"
                     /identity="100.0"
                     /match_length="8.3"
                     /fragment="True"
                     /other="repeat_region"
                     /description="AAV2 ITR Flip"
     CDS             772..828
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.3"
                     /fragment="True"
                     /other="CDS"
                     /description="lac repressor; lacI; The lac repressor binds
                     to the lac operator to inhibit transcription in E. coli.
                     This inhibition can be relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     CDS             1026..1094
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="98.6"
                     /match_length="3.2"
                     /fragment="True"
                     /other="CDS"
                     /description="large T antigen from SV40 (simian virus 40)"
     CDS             2849..2908
                     /note="pLannotate"
                     /label="KCNJ2_HUMAN (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="4.7"
                     /fragment="True"
                     /other="CDS"
                     /description="Protein evidence at protein level: Swiss-Prot
                     protein existence level 1. Inward rectifier potassium
                     channels are characterized by a greater tendency to allow
                     potassium to flow into the cell rather than out of it
                     (PubMed:36149965, PubMed:7590287, PubMed:9490857). Their
                     voltage dependence is regulated by the concentration of
                     extracellular potassium; as external potassium is raised,
                     the voltage range of the channel opening shifts to more
                     positive voltages (PubMed:7590287, PubMed:7696590). The
                     inward rectification is mainly due to the blockage of
                     outward current by internal magnesium (PubMed:9490857). Can
                     be blocked by extracellular barium or cesium
                     (PubMed:7590287, PubMed:7696590). Probably participates in
                     establishing action potential waveform and excitability of
                     neuronal and muscle tissues (PubMed:7590287,
                     PubMed:7696590, PubMed:7840300). From Homo sapiens
                     (Human)."
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg cgctcgctcg
      841 ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca
      901 gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttccttgtag
      961 ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa gatccttatc
     1021 gattttacca catttgtaga ggttttactt gctttaaaaa acctcccaca tctccccctg
     1081 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     1141 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     1201 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgctc gaagcggccg
     1261 cccgggtcga cggtatcgat gcggggaggc ggcccaaagg gagatccgac tcgtctgagg
     1321 gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc
     1381 cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc
     1441 aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca cggaattgtc
     1501 agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc
     1561 cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat
     1621 gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac
     1681 aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat
     1741 gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag
     1801 cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag
     1861 aggttgatta tcgataagct tgatatcgaa ttcgaacctc ttcgagggac ataacttcgt
     1921 ataatgtatg ctatacgaag ttatgggtcg atggtgagat ctggactaga gggtcgatgg
     1981 tgatgcttgg atcaaaatag gaagaatccc aatgataact tcgtataaag tatcctatac
     2041 gaagttatac tagaattcgg taccgctagc caccatggat ccaatagctc tccaggctgg
     2101 atacgacctg cttggagacg gacgaccaga gacactctgg cttggaattg gaactctgct
     2161 tatgcttatc gggacattct attttatagt aaaaggatgg ggagtaacag ataaggaggc
     2221 tcgggagtat tattccatta ctattctggt ccctggcatc gcatccgcag cttacctctc
     2281 aatgttcttc ggcatcggcc tcacagaagt gaccgtggca ggcgaggtgc tcgacattta
     2341 ctacgccagg tatgcagact ggctgttcac cacgcccctc ttgttgttgg atttggcact
     2401 gctggcaaag gtagaccgag tctccatcgg gaccttggtt ggtgtggatg ccctgatgat
     2461 cgtaacaggc ctcattggtg ccctctccca cacaccactc gcaaggtaca gctggtggct
     2521 tttcagtaca atttgtatga ttgtggtctt gtactttctc gcaacaagtc tgagggcagc
     2581 cgcaaaagaa aggggacctg aggtggccag cacctttaat accctgacgg ccctggttct
     2641 tgttctttgg acggcctatc cgatactctg gattattggt acagaaggcg ccggcgtcgt
     2701 gggcctgggc atcgagaccc tgctttttat ggtgctcgat gttaccgcta aggttggctt
     2761 cggattcatt ttgctgaggt caagagccat tttgggcgac acagaagctc ctgaacctgg
     2821 aaatggcaac ctcgagggca acgcagccaa gagccgaatc acgagcgaag gggaatacat
     2881 tcctttggat cagattgata tcaatgtggt gagtaagggg gaggaacttt tcactggcgt
     2941 cgtgcccatc ctggtggagc ttgatggcga tgtcaacggt cacaagttct ctgtatccgg
     3001 cgaaggagag ggggatgcta cctacggaaa attgactctt aaattcatct gtacaacggg
     3061 caaattgccc gtgccttggc cgacgctggt cactaccttc ggatacggtc tccagtgttt
     3121 tgctcgatat cctgatcata tgaagcagca tgacttcttt aaatcagcca tgccagaggg
     3181 atatgtccaa gagaggacca ttttctttaa ggatgatggt aactacaaga ctcgggccga
     3241 agtgaagttc gagggcgaca cactggtgaa ccggatcgag ctgaagggta tcgatttcaa
     3301 agaagatggt aacatcctgg gacataagct ggaatacaat tataacagtc acaatgttta
     3361 tatcatggct gataaacaga agaacgggat caaggtgaac ttcaagatcc ggcataacat
     3421 cgaagacggc tccgttcagc tggccgatca ctatcaacaa aacacaccaa tcggagatgg
     3481 ccccgtgctg ctccctgaca atcactacct gagttaccaa tctgcccttt caaaggaccc
     3541 taacgaaaag agggaccaca tggtgctcct ggaatttgtg actgccgcag ggattactct
     3601 cggcatggat gaactctata aattttgtta cgagaacgaa gtgtagggcg cgcctcgagt
     3661 gtacaagtaa ggggatccct agtataactt cgtatagcat acattatacg aagttatggg
     3721 tcgatggtga tgcttggcaa ttcgggtcga tggtgaagca ttggtcttcc tattttgata
     3781 taacttcgta taggatactt tatacgaagt tatggggatc caattctttg ccaaaatgat
     3841 gagacagcac aataaccagc acgttgccca ggagctgtag gaaaaagaag aaggcatgaa
     3901 catggttagc agaggctcta gagccgccgg tcacacgcca gaagccgaac cccgccctgc
     3961 cccgtccccc ccgaaggcag ccgtcccccc gcggacagcc ccgagccgct ccccccgcac
     4021 aaagggccct cccggagccc ctcaaggctt tcacgcagcc acagaaaaga aacaagccgt
     4081 cattaaacca agcgctaatt acagcccgga ggagaagggc cgtcccgccc gctcacctgt
     4141 gggagtaacg cggtcagtca gagccggggc gggcggcgcg aggcggcggc ggagcggggc
     4201 acggggcgaa ggcagcgcgc agcgactccc gcccgccgcg cgcttcgctt tttatagggc
     4261 cgccgccgcc gccgcctcgc cataaaagga aactttcgga gcgcgccgct ctgattggct
     4321 gccgccgcac ctctccgcct cgccccgccc cgcccctcgc cccgccccgc cccgcctggc
     4381 gcgcgccccc cccccccccc ccgcccccat cgctgcacaa aataattaaa aaataaataa
     4441 atacaaaatt gggggtgggg agggggggga gatggggaga gtgaagcaga acgtggggct
     4501 cacctcgacc atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc
     4561 ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat
     4621 agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa
     4681 atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt
     4741 cattattgac gtcaatggta ctcttcctag agcatggcta cgtagataag tagcatggcg
     4801 ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     4861 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     4921 cggcctcagt gagcgagcga gcgcgcagct ggcgtaatag cgaagaggcc cgcaccgatc
     4981 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     5041 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5101 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5161 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5221 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     5281 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     5341 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     5401 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     5461 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     5521 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     5581 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     5641 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     5701 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     5761 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5821 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5881 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5941 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6001 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6061 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6121 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     6181 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     6241 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6301 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     6361 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6421 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6481 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6541 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6601 agcgtcagac cccgtagaaa agatcaaagg atcttc
//
