LOCUS       pJMP2656-              10843 bp    DNA     circular SYN 23-OCT-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   John Hawkins
  TITLE     Direct Submission
  JOURNAL   Exported Oct 23, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10843
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     CDS             complement(6..105)
                     /codon_start=1
                     /label=3xMyc
                     /note="color: #993366"
                     /translation="GRGAETHL*RRSGTKVDFRRRSGTEAHL*GRSV"
     CDS             complement(join(108..1691,1692..1694,1695..4181,4182..4184,
                     4185..4211))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /note="This feature has 5 segments:
                        1:  108 .. 1691 / #993366
                        2: 1692 .. 1694 / #cc99b2
                        3: 1695 .. 4181 / #993366
                        4: 4182 .. 4184 / #cc99b2
                        5: 4185 .. 4211 / #993366"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    4212..4231
                     /label=RBS + SpeI
                     /note="strongest RBS from: Yang et al. Biotechnol Biofuels 
                     (2019) 12:52 https://doi.org/10.1186/s13068-019-1399-6 
                     modified by replacing the last 6 bases with a SpeI site."
                     /note="color: #a6acb3"
     misc_feature    complement(4232..4288)
                     /label=T7A1_O3O4
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    4234..4250
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     misc_feature    4260..4265
                     /label=-10
                     /note="color: #ffffff"
     protein_bind    4267..4283
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     misc_feature    4283..4288
                     /label=-35
                     /note="color: #ffffff"
     terminator      complement(4316..4364)
                     /label=pdhR-aceEF-lpd terminator
                     /note="color: #000000; direction: LEFT"
     terminator      complement(4365..4417)
                     /label=hisLGDCBHAFI terminator
                     /note="color: #000000; direction: LEFT"
     promoter        4484..4561
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
                     /note="color: #ffffff"
     CDS             4562..5644
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #993366"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     terminator      5736..5789
                     /label=garPLRK-rnpB terminator
                     /note="color: #000000; direction: RIGHT"
     terminator      5790..5846
                     /label=thrLABC terminator
                     /note="color: #000000; direction: RIGHT"
     misc_feature    5893..5914
                     /label=consensus UP
                     /note="from Estrem et al. 1998 PNAS; Identification of an
                     UP element consensus sequence forbacterial promoters"
                     /note="color: #a6acb3"
     protein_bind    5920..5939
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
                     /note="color: #31849b"
     ncRNA           5990..6082
                     /label=optimized sgRNA handle
                     /note="color: #8fbc8f; direction: RIGHT"
                     /ncRNA_class="other"
     terminator      6131..6181
                     /label=L3S1P13 terminator
                     /note="color: #000000; direction: RIGHT"
     terminator      6270..6359
                     /label=spy terminator
                     /note="color: #000000; direction: RIGHT"
     protein_bind    complement(6360..6407)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
                     /note="color: #31849b; direction: LEFT"
     promoter        join(6484..6489,6490..6506,6507..6512)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
                     /note="This forward directional feature has 3 segments:
                        1: 6484 .. 6489 / #ffffff / -35
                        2: 6490 .. 6506 / #ffffff
                        3: 6507 .. 6512 / #ffffff / -10"
     CDS             6595..7224
                     /codon_start=1
                     /label=cat (CmR)
                     /note="cat (Chloramphenicol resistance determinant)"
                     /note="color: #993366"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQ"
     protein_bind    complement(7292..7339)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
                     /note="color: #31849b; direction: LEFT"
     terminator      7390..7476
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
                     /note="color: #333333"
     terminator      complement(7579..7673)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
                     /note="color: #333333; direction: LEFT"
     mobile_element  7722..7919
                     /mobile_element_type="transposon:Tn7R"
                     /label=Tn7R
                     /note="color: #ffcc99"
     promoter        8397..8501
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             8502..9362
                     /codon_start=1
                     /transl_table=11
                     /gene="bla"
                     /product="Bla"
                     /label=bla
                     /note="color: #993366"
                     /db_xref="GI:403391486"
                     /protein_id="AFR43358.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9663..10051
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
                     /note="color: #ffff00"
     mobile_element  10532..10697
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
                     /note="color: #ffcc99"
     terminator      complement(10763..10817)
                     /label=L3S2P24 terminator
                     /note="L3S2P24 terminator"
                     /note="color: #000000; direction: LEFT"
ORIGIN
        1 ccttattaca gatcttcctc agagatgagc ttctgttcca gatcttcttc tgaaatcaac
       61 ttttgttcca gatcttcttc agagatgagt ttctgctccg cggccgcgtc acctcctagc
      121 tgactcaaat caatgcgtgt ttcataaaga ccagtgatgg attgatggat aagagtggca
      181 tctaaaactt cttttgtaga cgtatatcgt ttacgatcaa ttgttgtatc aaaatattta
      241 aaagcagcgg gagctccaag attcgtcaac gtaaataaat gaataatatt ttctgcttgt
      301 tcacgtattg gtttgtctct atgtttgtta tatgcactaa gaactttatc taaattggca
      361 tctgctaaaa taacacgctt agaaaattca ctgatttgct caataatctc atctaaataa
      421 tgcttatgct gctccacaaa caattgtttt tgttcgttat cttctggact acccttcaac
      481 ttttcataat gactagctaa atataaaaaa ttcacatatt tgcttggcag agccagctca
      541 tttccttttt gtaattctcc ggcactagcc agcatccgtt tacgaccgtt ttctaactca
      601 aaaagactat atttaggtag tttaatgatt aagtcttttt taacttcctt atatccttta
      661 gcttctaaaa agtcaatcgg atttttttca aaggaacttc tttccataat tgtgatccct
      721 agtaactctt taacggattt taacttcttc gatttccctt tttccacctt agcaaccact
      781 aggactgaat aagctaccgt tggactatca aaaccaccat atttttttgg atcccagtct
      841 tttttacgag caataagctt gtccgaattt ctttttggta aaattgactc cttggagaat
      901 ccgcctgtct gtacttctgt tttcttgaca atattgactt ggggcatgga caatactttg
      961 cgcactgtgg caaaatctcg ccctttatcc cagacaattt ctccagtttc cccattagtt
     1021 tcgattagag ggcgtttgcg aatctctcca tttgcaagtg taatttctgt tttgaagaag
     1081 ttcatgatat tagagtaaaa gaaatatttt gcggttgctt tgcctatttc ttgctcagac
     1141 ttagcaatca ttttacgaac atcataaact ttataatcac catagacaaa ctccgattca
     1201 agttttggat atttcttaat caaagcagtt ccaacgacgg catttagata cgcatcatgg
     1261 gcatgatggt aattgttaat ctcacgtact ttatagaatt ggaaatcttt tcggaagtca
     1321 gaaactaatt tagattttaa ggtaatcact ttaacctctc gaataagttt atcattttca
     1381 tcgtatttag tattcatgcg actatccaaa atttgtgcca catgcttagt gatttggcga
     1441 gtttcaacca attggcgttt gataaaacca gctttatcaa gttcactcaa acctccacgt
     1501 tcagctttcg ttaaattatc aaacttacgt tgagtgatta acttggcgtt tagaagttgt
     1561 ctccaatagt ttttcatctt tttgactact tcttcacttg gaacgttatc cgatttacca
     1621 cgatttttat cagaacgcgt taagacctta ttgtctattg aatcgtcttt aaggaaactt
     1681 tgtggaacaa tggcatcgac atcataatca cttaaacgat taatatctaa ttcttggtcc
     1741 acatacatgt ctcttccatt ttggagataa tagagataga gcttttcatt ttgcaattga
     1801 gtattttcaa caggatgctc tttaagaatc tgacttccta attctttgat accttcttcg
     1861 attcgtttca tacgctctcg cgaatttttc tggccctttt gagttgtctg attttcacgt
     1921 gccatttcaa taacgatatt ttctggctta tgccgcccca ttactttgac caattcatca
     1981 acaactttta cagtctgtaa aatacctttt ttaatagcag ggctaccagc taaatttgca
     2041 atatgttcat gtaaactatc gccttgtcca gacacttgtg ctttttgaat gtcttcttta
     2101 aatgtcaaac tatcatcatg gatcagctgc ataaaattgc gattggcaaa accatctgat
     2161 ttcaaaaaat ctaatattgt tttgccagat tgcttatccc taataccatt aatcaatttt
     2221 cgagacaaac gtccccaacc agtataacgg cgacgtttaa gctgtttcat caccttatca
     2281 tcaaagaggt gagcatatgt tttaagtctt tcctcaatca tctccctatc ttcaaataag
     2341 gtcaatgtta aaacaatatc ctctaagata tcttcatttt cttcattatc caaaaaatct
     2401 ttatctttaa taatttttag caaatcatgg taggtaccta atgaagcatt aaatctatct
     2461 tcaactcctg aaatttcaac actatcaaaa cattctattt ttttgaaata atcttctttt
     2521 aattgcttaa cggttacttt tcgatttgtt ttgaagagta aatcaacaat ggctttcttc
     2581 tgttcacctg aaagaaatgc tggttttcgc attccttcag taacatattt gacctttgtc
     2641 aattcgttat aaaccgtaaa atactcataa agcaaactat gttttggtag tactttttca
     2701 tttggaagat ttttatcaaa gtttgtcatg cgttcaataa atgattgagc tgaagcacct
     2761 ttatcgacaa cttcttcaaa attccatggg gtaattgttt cttcagactt ccgagtcatc
     2821 catgcaaaac gactattgcc acgcgccaat ggaccaacat aataagggat tcgaaaagtc
     2881 aagatttttt caatcttctc acgattgtct tttaaaaatg gataaaagtc ttcttgtctt
     2941 ctcaaaatag catgcagctc acccaagtga atttgatggg gaatagagcc gttgtcaaag
     3001 gtccgttgct tgcgcagcaa atcttcacga tttagtttca ccaataattc ctcagtacca
     3061 tccatttttt ctaaaattgg tttgataaat ttataaaatt cttcttggct agctccccca
     3121 tcaatataac ctgcatatcc gttttttgat tgatcaaaaa agatttcttt atacttttct
     3181 ggaagttgtt gtcgaactaa agcttttaaa agagtcaagt cttgatgatg ttcatcgtag
     3241 cgtttaatca ttgaagctga taggggagcc ttagttattt cagtatttac tcttaggata
     3301 tctgaaagta aaatagcatc tgataaattc ttagctgcca aaaacaaatc agcatattga
     3361 tctccaattt gcgccaataa attatctaaa tcatcatcgt aagtatcttt tgaaagctgt
     3421 aatttagcat cttctgccaa atcaaaattt gatttaaaat taggggtcaa acccaatgac
     3481 aaagcaatga gattcccaaa taagccattt ttcttctcac cggggagctg agcaatgaga
     3541 ttttctaatc gtcttgattt actcaatcgt gcagaaagaa tcgctttagc atctactcca
     3601 cttgcgttaa tagggttttc ttcaaataat tgattgtagg tttgtaccaa ctggataaat
     3661 agtttgtcca catcactatt atcaggattt aaatctccct caatcaaaaa atgaccacga
     3721 aacttaatca tatgcgctaa ggccaaatag attaagcgca aatccgcttt atcagtagaa
     3781 tctaccaatt tttttcgcag atgatagata gttggatatt tctcatgata agcaacttca
     3841 tctactatat ttccaaaaat aggatgacgt tcatgcttct tgtcttcttc caccaaaaaa
     3901 gactcttcaa gtcgatgaaa gaaactatca tctactttcg ccatctcatt tgaaaaaatc
     3961 tcctgtagat aacaaatacg attcttccga cgtgtatacc ttctacgagc tgtccgtttg
     4021 agacgagtcg cttccgctgt ctctccactg tcaaataaaa gagcccctat aagatttttt
     4081 ttgatactgt ggcggtctgt atttcccaga accttgaact ttttagacgg aaccttatat
     4141 tcatcagtga tcaccgccca tccgacgcta tttgtgccga tagctaagcc tattgagtat
     4201 ttcttatcca tactagtcct ccttctcgct caattgttat ccgctcacaa ttgaatctaa
     4261 gtatcattgt tatccgctca caagtcaaca ctctttttga taaattttac tagtctgcag
     4321 acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc cggagcgcaa taaaaaagcc
     4381 cccggaaggt gatcttccgg gggctttctc atgcgttgct tgtattgaaa atgggggcca
     4441 tgcctaccta tctgcctccc gggaagcggc atgcatttac gttgacacca tcgaatggtg
     4501 caaaaccttt cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa
     4561 tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt
     4621 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc
     4681 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca
     4741 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt
     4801 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga
     4861 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag
     4921 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg
     4981 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat
     5041 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca
     5101 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg
     5161 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
     5221 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac
     5281 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
     5341 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc
     5401 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
     5461 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
     5521 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
     5581 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     5641 gtgagcgcaa cgcaattaat gtaagttagc tcactcatta ggcaccccac ccgggtcctt
     5701 gagccccatt atcacctcca aagccttctc gtctggtcag tttcacctgt tttacgtaaa
     5761 aacccgcttc ggcgggtttt tacttttggg gaaacacaga aaaaagcccg cacctgacag
     5821 tgcgggcttt ttttttcgac caaaggtgcg actactcttg cctactacct atcgactgag
     5881 ctgaaagaat tcggaaaatt ttttttcaaa agtacttgaa attgtgagcg ctcacaatta
     5941 taattctagt agagaccaac tttggtctcc accatagcgg tcggtctctg tttaagagct
     6001 atgctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg
     6061 gcaccgagtc ggtgcttttt ttttgaattc atgtggctga ccgttctgtt gtctctcgct
     6121 cttccgagta gacgaacaat aaggcctccc taacgggggg ccttttttat tgataacaaa
     6181 agtcagtgct tccgctattt ccaaaatacc gggctaatac ggtttaaacg acctcctgga
     6241 tttgctcaga cagccttttc gtcattcgtt tcagccaaaa aacttaagac cgccggtctt
     6301 gtccactacc ttgcagtaat gcggtggaca ggatcggcgg ttttcttttc tcttctcaag
     6361 aagttcctat actttctaga gaataggaac ttcggaatag gaacttcctc ctgaacggcc
     6421 ataagaacga aggctgtctg ttgaactctc gagccgtgga aacggatgaa ggcacgaacc
     6481 cagttgacat aagcctgttc ggttcgtaaa ctgtaatgca agtagcgtat gcgctcatca
     6541 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag
     6601 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     6661 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
     6721 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
     6781 cttgcccgcc tgatgaatgc tcatccggag ttccgtatgg caatgaaaga cggtgagctg
     6841 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     6901 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     6961 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     7021 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     7081 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     7141 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     7201 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaatcgact
     7261 ggaactcgag catcagcttc aaaagcgctc tgaagttcct atactttcta gagaatagga
     7321 acttcggaat aggtacttca agatccccaa ttcgagctca tcgtccgggc cgcaagctcc
     7381 tagcggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     7441 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctcaagctag agagtcatta
     7501 ccccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact
     7561 catcgcagtc tagcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     7621 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagctc
     7681 agctaattaa gctagcttat cgataccgtc gacctcgaac cccacgcccc tctttaatac
     7741 gacgggcaat ttgcacttca gaaaatgaag agtttgcttt agccataaca aaagtccagt
     7801 atgctttttc acagcataac tggactgatt tcagtttaca actattctgt ctagtttaag
     7861 actttattgt catagtttag atctattttg ttcagtttaa gactttattg tccgcccaca
     7921 cccgcttacg cagggcatcc atttattact caaccgtaac cgattttgcc aggttacgcg
     7981 gctggtcctc tagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     8041 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     8101 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     8161 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     8221 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     8281 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     8341 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     8401 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8461 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8521 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     8581 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     8641 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     8701 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     8761 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8821 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     8881 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     8941 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9001 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9061 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9121 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9181 ggtttattgc tgataaatct ggagccggtg agcgtgggtc ccgcggtatc attgcagcac
     9241 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9301 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9361 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9421 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9481 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggcagt tattggtgcc
     9541 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     9601 ttaaaacttc atttttaatt tttgcggccg caagatccgg ccacgatgcg tccggcgtag
     9661 aggatctgaa gatcagcagt tcaacctgtt gatagtacgt actaagctct catgtttcac
     9721 gtactaagct ctcatgttta acgtactaag ctctcatgtt taacgaacta aaccctcatg
     9781 gctaacgtac taagctctca tggctaacgt actaagctct catgtttcac gtactaagct
     9841 ctcatgtttg aacaataaaa ttaatataaa tcagcaactt aaatagcctc taaggtttta
     9901 agttttataa gaaaaaaaag aatatataag gcttttaaag cttttaaggt ttaacggttg
     9961 tggacaacaa gccagggatg taacgcactg agaagccctt agagcctctc aaagcaattt
    10021 tgagtgacac aggaacactt aacggctgac atgggaatta gccatggcat cacagtatcg
    10081 tgatgacaga ggcagggagt gcggcctttt tacggttcct ggccttttgc tggccttttg
    10141 ctcacatgtt ctttcctgcg ttatcagggg attccttaag gtatactttc cgctgcataa
    10201 ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat
    10261 acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg
    10321 ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat
    10381 tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccgat aagctctgat
    10441 accgctcgcc gcagccgaac gaccgagcgc agcgggtcag tgagcgagga agcggaagag
    10501 cccaaacata acaggaagaa aaatgccccg ctgtgggcgg acaaaatagt tgggaactgg
    10561 gaggggtgga aatggagttt ttaaggatta tttagggaag agtgacaaaa tagatgggaa
    10621 ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg acaaaatagt ttggaactag
    10681 atttcactta tctggttctt gaggcggtta aaagagccgt actcttctcc gatgtcgact
    10741 aggccatgat gctcattctg tgggaccaaa acgaaaaaac accctttcgg gtgtcttttc
    10801 tggaatttgg taccgaggct gcaagtccca ttaaggaggc gcg
//
