LOCUS       Exported                8057 bp ds-DNA     circular SYN 17-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   giselle
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 24, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..120
                     /label=PBfP1E6
     regulatory      125..206
                     /regulatory_class="ribosome_binding_site"
                     /label=RBS1p-LP
     CDS             207..896
                     /codon_start=1
                     /label=mScarlet-I3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        989..1460
                     /label=PopuA
     CDS             1461..2084
                     /codon_start=1
                     /label=TetR
                     /note="TetR"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     terminator      2105..2133
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     misc_feature    2164..2271
                     /label=pTetR56
     protein_bind    2249..2267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2272..2353
                     /label=RBS1P-LP
     CDS             2354..3073
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     terminator      3094..3121
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(3371..4654)
                     /codon_start=1
                     /transl_table=11
                     /product="NBU integrase"
                     /label=NBU integrase
                     /protein_id="ARI71345.1"
                     /translation="MNIKRNIIFALESRKKNGVPIVENVPIRMRVIFASQRIEFTTGYR
                     IDVAKWDADKQRVKNGCTNKLKQSAAEINTDLLKYYAEIQNIFKEFEVQEVMPTTQQLK
                     EAFNMRMKDTSEEQPEEAPVSFWEVFDEFVKECGNQNNWTASTYEKFAAVRNHLKEFKE
                     DATFNYFNEFGLNEYVNFLRDTKDMRNSTIGKQMGFLKWFLRWSFKKGHHQNIAYDTFK
                     PKLKTTSKKVIFLTWDELNKLKDYQIPKDKQYLERVRDVFLFCCFTSLRYSDVRNLKRS
                     DVKSDHIEITTVKTADSLTIELNKYSKAILDKYKDIHFENYMALPVISNQKMNDYLKEL
                     GELAEINEPVRETYYKGNERIDEVTHKYALLSTHAGKRTFICNALALGIPAQVVMKWTG
                     HSDYKAMKPYIDIADDIKANAMNKFNQL"
     CDS             complement(5100..5960)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /label=AmpR
                     /protein_id="ARI71346.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             6448..7182
                     /codon_start=1
                     /transl_table=11
                     /product="ErmG"
                     /label=ErmG
                     /protein_id="ARI71348.1"
                     /translation="MNKVNIKDSQNFITSKYHIEKIMNCISLDEKDNIFEIGAGKGHFT
                     AGLVKRCNFVTAIEIDSKLCEVTRNKLLNYPNYQIVNDDILKFTFPSHNPYKIFGNIPY
                     NISTNIIRKIVFESSATISYLIVEYGFAKMLLDTNRSLALLLMAEVDISILAKIPRYYF
                     HPKPKVDSTLIVLKRKPAKMAFKERKKYETFVMKWVNKEYEKLFTKNQFNKALKHARIY
                     DINNISFEQFVSLFNSYKIFNG"
     rep_origin      7252..7640
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            7811..7920
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgaat aaatctagtt tagttattga cgccaaatat
     1021 acggtgttta agtgaaatat tcaaacttat tttatattta tctgccggtt tgttctattt
     1081 tgctgagtga taggggaata ttacccggaa actttccata tcattacaaa taaagccctc
     1141 tgtatgcccc ggattttgaa tataatttgt atgtttaatc cgttttttgt atctttgtgc
     1201 cagcaagata ctggaacagg ctgtttttat acataaaaat gctatttttt gctattcgtt
     1261 gaaaggatta tcttatcaaa ttctattcct tttttaatta ctcagtctgt caataatcat
     1321 tttattaatt atttcgggat tcattgttat ttgtccctta tttaaatcag aactttatac
     1381 gatataaaga aagattcctg ttatcgtttg ttcttgtctt gctgtgtata ttattttaat
     1441 aacctaaacc attttattgt atgtccagat tagataaaag taaagtgatt aacagcgcat
     1501 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
     1561 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
     1621 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
     1681 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
     1741 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
     1801 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
     1861 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
     1921 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
     1981 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
     2041 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc ttaatggcca catcggttcc
     2101 tgctagaagg cccatcctga cggatggcct tttggtattc gagcattacc tcatactttg
     2161 gatttttgtt ttgtttgcaa tggttaatct attgttaaaa tttaaagttt cacttgaact
     2221 ttcaaataat gttcttatat ttgcagtgtc cctatcagtg atagagagta ggatcacaca
     2281 acatttaaaa aataacatta tgaaagcact cgaaaagaga ttcaaaggta atattaacaa
     2341 taatttattt tcaatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2401 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtccgcggcg agggcgaggg
     2461 cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     2521 gccctggccc accctcgtga ccaccttagg ctacggcgtg gcctgcttcg cccgctaccc
     2581 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     2641 gcgcaccatc tctttcaagg acgacggtac ctacaagacc cgcgccgagg tgaagttcga
     2701 gggcgacacc ctggtgaacc gcatcgtgct gaagggcatc gacttcaagg aggacggcaa
     2761 catcctgggg cacaagctgg agtacaactt caacagccac aaggtctata tcacggccga
     2821 caagcagaag aacggcatca aggctaactt caagacccgc cacaacgttg aggacggcgg
     2881 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     2941 gcccgacaac cactacctga gccatcagtc caagctgagc aaagacccca acgagaagcg
     3001 cgatcacatg gtcctgaagg agagggtgac cgccgccggg attacacatg acatggacga
     3061 gctgtacaag taatagcgtt cagctcgact gtgagaaggc catcctgacg gatggccttt
     3121 tttttcgtta taactagttg cgttcctagc cgctactagt gtaattgcct atcttccagt
     3181 gatggaacag catttgtgca ttggctgcaa caatcagcct tacttgtgcc tgttctattt
     3241 ccgaaccgac cgcttgtatg aatgcatcaa aattcgtttt ctctacgttg gattccttgt
     3301 tgctcatatt gtgatgataa tttctacaaa tatagtcatt ggtaactatc tatgaaactg
     3361 tttgatactt ttatagttga ttaaacttgt tcatggcatt tgccttaata tcatccgcta
     3421 tgtcaatgta gggtttcata gctttgtagt cgctgtgtcc cgtccatttc atgaccacct
     3481 gtgccgggat tccgagagcc agcgcattgc agatgaatgt cctttttcct gcatgggtac
     3541 tgagcaaagc gtatttgtgt gtgacttcat caatacgttc atttcccttg tagtaggttt
     3601 cccgtacagg ctcgttgatt tctgccagtt cgcccagctc tttcaggtaa tcgttcatct
     3661 tctggttgct gatgacgggc agagccatgt aattctcgaa atggatgtcc ttgtatttgt
     3721 ccagtatggc tttgctgtat ttgttcagtt caatcgtcag gctgtcggca gtcttgactg
     3781 tggttatttc gatgtggtcg gacttcacat cgcttctttt cagattgcga acatccgaat
     3841 accgcaaact cgtaaagcag cagaacagga aaacatcacg cacacgttcc aggtattgct
     3901 tatccttggg tatctggtag tctttcagct tgttcagttc atcccaagtc aggaagatta
     3961 cttttttcga ggtggttttc agtttcggtt tgaacgtatc gtatgcaatg ttctgatgat
     4021 gtcctttctt gaagctccag cgcaggaacc atttgaggaa tcccatttgc ttgccgatgg
     4081 tgctgtttct catatccttg gtgtcacgca ggaagttgac gtattcgttc aatccaaact
     4141 cgttgaaata gttgaacgtt gcatcctcct tgaactcttt gaggtggttc ctcactgctg
     4201 caaatttttc ataggtggat gccgtccagt tattctggtt accgcactct tttacaaact
     4261 catcgaacac ctcccaaaag ctgacagggg cttcttccgg ctgttcttca ctggtgtctt
     4321 tcattctcat gttgaaagct tccttcaact gttgggtcgt tggcatgacc tcctgcacct
     4381 caaattcctt gaaaatattc tggatttcgg catagtattt cagcaagtcc gtattgattt
     4441 cggctgcact ttgctttagc ttgttggtac atccgttctt tacccgctgc ttatctgcat
     4501 cccatttggc tacgtcaatc cggtagcccg ttgtaaactc gatacgttgg ctggcaaaga
     4561 tgacacgcat acggatgggt acgttctcta cgattggcac accgttcttt ttccggctct
     4621 ccaatgcaaa aatgatgttg cgcttgatat tcataattgg gtgcgtttga aattctacac
     4681 ccaaatatac acccaattat tgagatagca aaagacattt agaaacattt acttttactc
     4741 tatattgtaa tttacacttg attatcagtc gtttgcagtt ttatgatatt ctgtgaaagt
     4801 ataagttcga gagcctgtct ctccgcaaaa aacgctgaaa atcagcagat tgcaaaacaa
     4861 acaccctgtt ttacacccaa gaatgtaaag tcgggtgttt ttgttttatt taagataata
     4921 caaccactac ataataaaag agtagcgata ttaaaagaat ccgatgagaa aagactaata
     4981 tttatctatc cattcagttt gatttctcag gactttacat cgtcctgaaa gtatttgttg
     5041 gtaccggtac cgaggacgcg acagctgcag acgcgttatg agtaaacttg gtctgacagt
     5101 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     5161 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     5221 agtgctgcaa tgataccgcg ggacccacgc tcaccggctc cagatttatc agcaataaac
     5281 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     5341 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     5401 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     5461 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     5521 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     5581 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     5641 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     5701 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     5761 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     5821 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     5881 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     5941 cggaaatgtt gaatactcat actcttcctt tttcaatcat ggccgcggga ttaaaagtcg
     6001 gggattggtg aacaaaaagg tgtttctctc tttaagagaa atatcgtttt gctaaacagt
     6061 tgatattgag gtatcatttt atcgtaaaag acatttttgc tcaacaattg cttgacggaa
     6121 atcaacaaat tttagcattt tgtaaaaaag tcgctatata atttggtgaa ttggagttat
     6181 tttcatattt ttgcatcccg aagagtttct cttaaagaga gaaacatctt ttgcatacct
     6241 tttccgaccg aatttttatg tcgtaaagag gggctttgca gggggtggac tcagaaagat
     6301 gagaatagat gactattgta gttgaaacac atagaaagtt gctgatatac agaccgatac
     6361 gcatatcggg atgaaccatg agtacgttct tttctcaaaa aacataaata ttcgaaaaga
     6421 gatgcaataa attaaggaga ggttataatg aacaaagtaa atataaaaga tagtcaaaat
     6481 tttattactt caaaatatca catagaaaaa ataatgaatt gcataagttt agatgaaaaa
     6541 gataacatct ttgaaatagg tgcagggaaa ggtcatttta ctgctggatt ggtaaagaga
     6601 tgtaattttg taacggcgat agaaattgat tctaaattat gtgaggtaac tcgtaataag
     6661 ctcttaaatt atcctaacta tcaaatagta aatgatgata tactgaaatt tacatttcct
     6721 agccacaatc catataaaat atttggcaac ataccttaca acataagcac aaatataatt
     6781 cgaaaaattg tttttgaaag ttcagccaca ataagttatt taatagtgga atatggtttt
     6841 gctaaaatgt tattagatac aaacagatca ctagcattgc tgttaatggc agaggtagat
     6901 atttctatat tagcaaaaat tcctaggtat tatttccatc caaaacctaa agtggatagc
     6961 acattaattg tattaaaaag aaagccagca aaaatggcat ttaaagagag aaaaaaatat
     7021 gaaacttttg taatgaaatg ggttaacaaa gagtacgaaa aactgtttac aaaaaatcaa
     7081 tttaataaag ctttaaaaca tgcgagaata tatgatataa acaatattag tttcgaacaa
     7141 tttgtatcgc tatttaatag ttataaaata tttaacggct aaaaacaata ggccacatgc
     7201 aactgtaaat gtttacgcgg gtaccgacac cgcggtggag gggaattccc atgtcagccg
     7261 ttaagtgttc ctgtgtcact caaaattgct ttgagaggct ctaagggctt ctcagtgcgt
     7321 tacatccctg gcttgttgtc cacaaccgtt aaaccttaaa agctttaaaa gccttatata
     7381 ttcttttttt tcttataaaa cttaaaacct tagaggctat ttaagttgct gatttatatt
     7441 aattttattg ttcaaacatg agagcttagt acgtgaaaca tgagagctta gtacgttagc
     7501 catgagagct tagtacgtta gccatgaggg tttagttcgt taaacatgag agcttagtac
     7561 gttaaacatg agagcttagt acgtgaaaca tgagagctta gtacgtacta tcaacaggtt
     7621 gaactgctga tcttcagatc ctctacgccg gacgcatcgt ggccggatca attccgtttt
     7681 ccgctgcata accctgcttc ggggtcatta tagcgatttt ttcggtatat ccatcctttt
     7741 tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga ctttccttgg tgtatccaac
     7801 ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc gagcgggtgt tccttcttca
     7861 ctgtccctta ttcgcacctg gcggtgctca acgggaatcc tgctctgcga ggctggccgg
     7921 ctaccgccgg cgtaacagat gagggcaagc ggatggctga tgaaaccaag ccaaccagga
     7981 agggcagccc acctatcacg gaattgatcc ccctcgaatt ggaattctag cctggaatag
     8041 ggaagcacca catcacc
//
