LOCUS       Exported                8721 bp ds-DNA     circular SYN 17-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   giselle
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 24, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..120
                     /label=Promoter P_BfP1E6
     regulatory      125..206
                     /regulatory_class="ribosome_binding_site"
                     /label=RBS1p-LP
     CDS             207..896
                     /codon_start=1
                     /label=mScarlet-I3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    1023..1041
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1070..1088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1119..1137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1151..1169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1193..1211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1239..1257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1281..1299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1329..1347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1378..1396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1420..1438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      1473..1544
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1560..1587
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        1596..2124
                     /label=PopuA
     CDS             2125..2748
                     /codon_start=1
                     /label=TetR
                     /note="TetR"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     terminator      2769..2797
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     misc_feature    2828..2935
                     /label=pTetR56
     protein_bind    2913..2931
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2936..3017
                     /label=RBS1P-LP
     CDS             3018..3737
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     terminator      3758..3785
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(4035..5318)
                     /codon_start=1
                     /transl_table=11
                     /product="NBU integrase"
                     /label=NBU integrase
                     /protein_id="ARI71345.1"
                     /translation="MNIKRNIIFALESRKKNGVPIVENVPIRMRVIFASQRIEFTTGYR
                     IDVAKWDADKQRVKNGCTNKLKQSAAEINTDLLKYYAEIQNIFKEFEVQEVMPTTQQLK
                     EAFNMRMKDTSEEQPEEAPVSFWEVFDEFVKECGNQNNWTASTYEKFAAVRNHLKEFKE
                     DATFNYFNEFGLNEYVNFLRDTKDMRNSTIGKQMGFLKWFLRWSFKKGHHQNIAYDTFK
                     PKLKTTSKKVIFLTWDELNKLKDYQIPKDKQYLERVRDVFLFCCFTSLRYSDVRNLKRS
                     DVKSDHIEITTVKTADSLTIELNKYSKAILDKYKDIHFENYMALPVISNQKMNDYLKEL
                     GELAEINEPVRETYYKGNERIDEVTHKYALLSTHAGKRTFICNALALGIPAQVVMKWTG
                     HSDYKAMKPYIDIADDIKANAMNKFNQL"
     CDS             complement(5764..6624)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /label=AmpR
                     /protein_id="ARI71346.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             7112..7846
                     /codon_start=1
                     /transl_table=11
                     /product="ErmG"
                     /label=ErmG
                     /protein_id="ARI71348.1"
                     /translation="MNKVNIKDSQNFITSKYHIEKIMNCISLDEKDNIFEIGAGKGHFT
                     AGLVKRCNFVTAIEIDSKLCEVTRNKLLNYPNYQIVNDDILKFTFPSHNPYKIFGNIPY
                     NISTNIIRKIVFESSATISYLIVEYGFAKMLLDTNRSLALLLMAEVDISILAKIPRYYF
                     HPKPKVDSTLIVLKRKPAKMAFKERKKYETFVMKWVNKEYEKLFTKNQFNKALKHARIY
                     DINNISFEQFVSLFNSYKIFNG"
     rep_origin      7916..8304
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            8475..8584
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgagc ggccgcttct agagatcgat tttagagctg
     1021 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     1081 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     1141 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     1201 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     1261 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     1321 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     1381 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     1441 taggtgtccc tagaattact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1501 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1561 gctcaccttc gggtgggcct ttctgcgttt atatatgatg tgtaatgaca attatccttc
     1621 cccaggaaaa ttgtaatagc cctgttgaac atataaatct agtttagtta ttgacgccaa
     1681 atatacggtg tttaagtgaa atattcaaac ttattttata tttatctgcc ggtttgttct
     1741 attttgctga gtgatagggg aatattaccc ggaaactttc catatcatta caaataaagc
     1801 cctctgtatg ccccggattt tgaatataat ttgtatgttt aatccgtttt ttgtatcttt
     1861 gtgccagcaa gatactggaa caggctgttt ttatacataa aaatgctatt ttttgctatt
     1921 cgttgaaagg attatcttat caaattctat tcctttttta attactcagt ctgtcaataa
     1981 tcattttatt aattatttcg ggattcattg ttatttgtcc cttatttaaa tcagaacttt
     2041 atacgatata aagaaagatt cctgttatcg tttgttcttg tcttgctgtg tatattattt
     2101 taataaccta aaccatttta ttgtatgtcc agattagata aaagtaaagt gattaacagc
     2161 gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag
     2221 aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc
     2281 gacgccttag ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg
     2341 gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt
     2401 catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact
     2461 ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat
     2521 gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa
     2581 gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa
     2641 gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg
     2701 atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtcttaatg gccacatcgg
     2761 ttcctgctag aaggcccatc ctgacggatg gccttttggt attcgagcat tacctcatac
     2821 tttggatttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa gtttcacttg
     2881 aactttcaaa taatgttctt atatttgcag tgtccctatc agtgatagag agtaggatca
     2941 cacaacattt aaaaaataac attatgaaag cactcgaaaa gagattcaaa ggtaatatta
     3001 acaataattt attttcaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3061 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg
     3121 agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     3181 ccgtgccctg gcccaccctc gtgaccacct taggctacgg cgtggcctgc ttcgcccgct
     3241 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     3301 aggagcgcac catctctttc aaggacgacg gtacctacaa gacccgcgcc gaggtgaagt
     3361 tcgagggcga caccctggtg aaccgcatcg tgctgaaggg catcgacttc aaggaggacg
     3421 gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaaggtc tatatcacgg
     3481 ccgacaagca gaagaacggc atcaaggcta acttcaagac ccgccacaac gttgaggacg
     3541 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     3601 tgctgcccga caaccactac ctgagccatc agtccaagct gagcaaagac cccaacgaga
     3661 agcgcgatca catggtcctg aaggagaggg tgaccgccgc cgggattaca catgacatgg
     3721 acgagctgta caagtaatag cgttcagctc gactgtgaga aggccatcct gacggatggc
     3781 cttttttttc gttataacta gttgcgttcc tagccgctac tagtgtaatt gcctatcttc
     3841 cagtgatgga acagcatttg tgcattggct gcaacaatca gccttacttg tgcctgttct
     3901 atttccgaac cgaccgcttg tatgaatgca tcaaaattcg ttttctctac gttggattcc
     3961 ttgttgctca tattgtgatg ataatttcta caaatatagt cattggtaac tatctatgaa
     4021 actgtttgat acttttatag ttgattaaac ttgttcatgg catttgcctt aatatcatcc
     4081 gctatgtcaa tgtagggttt catagctttg tagtcgctgt gtcccgtcca tttcatgacc
     4141 acctgtgccg ggattccgag agccagcgca ttgcagatga atgtcctttt tcctgcatgg
     4201 gtactgagca aagcgtattt gtgtgtgact tcatcaatac gttcatttcc cttgtagtag
     4261 gtttcccgta caggctcgtt gatttctgcc agttcgccca gctctttcag gtaatcgttc
     4321 atcttctggt tgctgatgac gggcagagcc atgtaattct cgaaatggat gtccttgtat
     4381 ttgtccagta tggctttgct gtatttgttc agttcaatcg tcaggctgtc ggcagtcttg
     4441 actgtggtta tttcgatgtg gtcggacttc acatcgcttc ttttcagatt gcgaacatcc
     4501 gaataccgca aactcgtaaa gcagcagaac aggaaaacat cacgcacacg ttccaggtat
     4561 tgcttatcct tgggtatctg gtagtctttc agcttgttca gttcatccca agtcaggaag
     4621 attacttttt tcgaggtggt tttcagtttc ggtttgaacg tatcgtatgc aatgttctga
     4681 tgatgtcctt tcttgaagct ccagcgcagg aaccatttga ggaatcccat ttgcttgccg
     4741 atggtgctgt ttctcatatc cttggtgtca cgcaggaagt tgacgtattc gttcaatcca
     4801 aactcgttga aatagttgaa cgttgcatcc tccttgaact ctttgaggtg gttcctcact
     4861 gctgcaaatt tttcataggt ggatgccgtc cagttattct ggttaccgca ctcttttaca
     4921 aactcatcga acacctccca aaagctgaca ggggcttctt ccggctgttc ttcactggtg
     4981 tctttcattc tcatgttgaa agcttccttc aactgttggg tcgttggcat gacctcctgc
     5041 acctcaaatt ccttgaaaat attctggatt tcggcatagt atttcagcaa gtccgtattg
     5101 atttcggctg cactttgctt tagcttgttg gtacatccgt tctttacccg ctgcttatct
     5161 gcatcccatt tggctacgtc aatccggtag cccgttgtaa actcgatacg ttggctggca
     5221 aagatgacac gcatacggat gggtacgttc tctacgattg gcacaccgtt ctttttccgg
     5281 ctctccaatg caaaaatgat gttgcgcttg atattcataa ttgggtgcgt ttgaaattct
     5341 acacccaaat atacacccaa ttattgagat agcaaaagac atttagaaac atttactttt
     5401 actctatatt gtaatttaca cttgattatc agtcgtttgc agttttatga tattctgtga
     5461 aagtataagt tcgagagcct gtctctccgc aaaaaacgct gaaaatcagc agattgcaaa
     5521 acaaacaccc tgttttacac ccaagaatgt aaagtcgggt gtttttgttt tatttaagat
     5581 aatacaacca ctacataata aaagagtagc gatattaaaa gaatccgatg agaaaagact
     5641 aatatttatc tatccattca gtttgatttc tcaggacttt acatcgtcct gaaagtattt
     5701 gttggtaccg gtaccgagga cgcgacagct gcagacgcgt tatgagtaaa cttggtctga
     5761 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5821 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5881 ccccagtgct gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat
     5941 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6001 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6061 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6121 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6181 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6241 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6301 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6361 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6421 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6481 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6541 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6601 gacacggaaa tgttgaatac tcatactctt cctttttcaa tcatggccgc gggattaaaa
     6661 gtcggggatt ggtgaacaaa aaggtgtttc tctctttaag agaaatatcg ttttgctaaa
     6721 cagttgatat tgaggtatca ttttatcgta aaagacattt ttgctcaaca attgcttgac
     6781 ggaaatcaac aaattttagc attttgtaaa aaagtcgcta tataatttgg tgaattggag
     6841 ttattttcat atttttgcat cccgaagagt ttctcttaaa gagagaaaca tcttttgcat
     6901 accttttccg accgaatttt tatgtcgtaa agaggggctt tgcagggggt ggactcagaa
     6961 agatgagaat agatgactat tgtagttgaa acacatagaa agttgctgat atacagaccg
     7021 atacgcatat cgggatgaac catgagtacg ttcttttctc aaaaaacata aatattcgaa
     7081 aagagatgca ataaattaag gagaggttat aatgaacaaa gtaaatataa aagatagtca
     7141 aaattttatt acttcaaaat atcacataga aaaaataatg aattgcataa gtttagatga
     7201 aaaagataac atctttgaaa taggtgcagg gaaaggtcat tttactgctg gattggtaaa
     7261 gagatgtaat tttgtaacgg cgatagaaat tgattctaaa ttatgtgagg taactcgtaa
     7321 taagctctta aattatccta actatcaaat agtaaatgat gatatactga aatttacatt
     7381 tcctagccac aatccatata aaatatttgg caacatacct tacaacataa gcacaaatat
     7441 aattcgaaaa attgtttttg aaagttcagc cacaataagt tatttaatag tggaatatgg
     7501 ttttgctaaa atgttattag atacaaacag atcactagca ttgctgttaa tggcagaggt
     7561 agatatttct atattagcaa aaattcctag gtattatttc catccaaaac ctaaagtgga
     7621 tagcacatta attgtattaa aaagaaagcc agcaaaaatg gcatttaaag agagaaaaaa
     7681 atatgaaact tttgtaatga aatgggttaa caaagagtac gaaaaactgt ttacaaaaaa
     7741 tcaatttaat aaagctttaa aacatgcgag aatatatgat ataaacaata ttagtttcga
     7801 acaatttgta tcgctattta atagttataa aatatttaac ggctaaaaac aataggccac
     7861 atgcaactgt aaatgtttac gcgggtaccg acaccgcggt ggaggggaat tcccatgtca
     7921 gccgttaagt gttcctgtgt cactcaaaat tgctttgaga ggctctaagg gcttctcagt
     7981 gcgttacatc cctggcttgt tgtccacaac cgttaaacct taaaagcttt aaaagcctta
     8041 tatattcttt tttttcttat aaaacttaaa accttagagg ctatttaagt tgctgattta
     8101 tattaatttt attgttcaaa catgagagct tagtacgtga aacatgagag cttagtacgt
     8161 tagccatgag agcttagtac gttagccatg agggtttagt tcgttaaaca tgagagctta
     8221 gtacgttaaa catgagagct tagtacgtga aacatgagag cttagtacgt actatcaaca
     8281 ggttgaactg ctgatcttca gatcctctac gccggacgca tcgtggccgg atcaattccg
     8341 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc
     8401 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc
     8461 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc
     8521 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg
     8581 ccggctaccg ccggcgtaac agatgagggc aagcggatgg ctgatgaaac caagccaacc
     8641 aggaagggca gcccacctat cacggaattg atccccctcg aattggaatt ctagcctgga
     8701 atagggaagc accacatcac c
//
