LOCUS       Exported                9181 bp ds-DNA     circular SYN 17-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9181)
  AUTHORS   giselle
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 24, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9181
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..120
                     /label=Promoter P_BfP1E6
     RBS             125..206
                     /label=RBS1P-LP
     CDS             207..896
                     /codon_start=1
                     /label=mScarlet-I3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    1023..1041
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1070..1088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1119..1137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1151..1169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1193..1211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1239..1257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1281..1299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1329..1347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1378..1396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1420..1438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1483..1501
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1530..1548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1579..1597
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1611..1629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1653..1671
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1699..1717
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1741..1759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1789..1807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1838..1856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1880..1898
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      1933..2004
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2020..2047
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        2056..2584
                     /label=PopuA
     CDS             2585..3208
                     /codon_start=1
                     /label=TetR
                     /note="TetR"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     terminator      3229..3257
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     misc_feature    3288..3395
                     /label=pTetR56
     protein_bind    3373..3391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             3396..3477
                     /label=RBS1P-LP
     CDS             3478..4197
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     terminator      4218..4245
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(4495..5778)
                     /codon_start=1
                     /transl_table=11
                     /product="NBU integrase"
                     /label=NBU integrase
                     /protein_id="ARI71345.1"
                     /translation="MNIKRNIIFALESRKKNGVPIVENVPIRMRVIFASQRIEFTTGYR
                     IDVAKWDADKQRVKNGCTNKLKQSAAEINTDLLKYYAEIQNIFKEFEVQEVMPTTQQLK
                     EAFNMRMKDTSEEQPEEAPVSFWEVFDEFVKECGNQNNWTASTYEKFAAVRNHLKEFKE
                     DATFNYFNEFGLNEYVNFLRDTKDMRNSTIGKQMGFLKWFLRWSFKKGHHQNIAYDTFK
                     PKLKTTSKKVIFLTWDELNKLKDYQIPKDKQYLERVRDVFLFCCFTSLRYSDVRNLKRS
                     DVKSDHIEITTVKTADSLTIELNKYSKAILDKYKDIHFENYMALPVISNQKMNDYLKEL
                     GELAEINEPVRETYYKGNERIDEVTHKYALLSTHAGKRTFICNALALGIPAQVVMKWTG
                     HSDYKAMKPYIDIADDIKANAMNKFNQL"
     CDS             complement(6224..7084)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /label=AmpR
                     /protein_id="ARI71346.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             7572..8306
                     /codon_start=1
                     /transl_table=11
                     /product="ErmG"
                     /label=ErmG
                     /protein_id="ARI71348.1"
                     /translation="MNKVNIKDSQNFITSKYHIEKIMNCISLDEKDNIFEIGAGKGHFT
                     AGLVKRCNFVTAIEIDSKLCEVTRNKLLNYPNYQIVNDDILKFTFPSHNPYKIFGNIPY
                     NISTNIIRKIVFESSATISYLIVEYGFAKMLLDTNRSLALLLMAEVDISILAKIPRYYF
                     HPKPKVDSTLIVLKRKPAKMAFKERKKYETFVMKWVNKEYEKLFTKNQFNKALKHARIY
                     DINNISFEQFVSLFNSYKIFNG"
     rep_origin      8376..8764
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            8935..9044
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgagc ggccgcttct agagatcgat tttagagctg
     1021 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     1081 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     1141 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     1201 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     1261 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     1321 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     1381 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     1441 taggtgtccc tagaattact agagatcgat tttagagctg cctccctatc agtgatagag
     1501 attgctacaa ctgcaaggta gtcccggtat ccctatcagt gatagagact gatagctacg
     1561 agtctgagat cctacttgtc cctatcagtg atagagacag taccgagttt tccctatcag
     1621 tgatagagag ctcatactgc gacagagtct tttccctatc agtgatagag ataggctcct
     1681 ttgtcaaaag tgatcgcttc cctatcagtg atagagactt cacgggataa tgtgttctac
     1741 tccctatcag tgatagagag cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg
     1801 atagagaaac tccgtcatcg tggatgtcaa tactgactcc ctatcagtga tagagattga
     1861 tttcaccgct ggcgtcaatt ccctatcagt gatagagact taggtgtccc tagaattact
     1921 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1981 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     2041 ttctgcgttt atatatgatg tgtaatgaca attatccttc cccaggaaaa ttgtaatagc
     2101 cctgttgaac atataaatct agtttagtta ttgacgccaa atatacggtg tttaagtgaa
     2161 atattcaaac ttattttata tttatctgcc ggtttgttct attttgctga gtgatagggg
     2221 aatattaccc ggaaactttc catatcatta caaataaagc cctctgtatg ccccggattt
     2281 tgaatataat ttgtatgttt aatccgtttt ttgtatcttt gtgccagcaa gatactggaa
     2341 caggctgttt ttatacataa aaatgctatt ttttgctatt cgttgaaagg attatcttat
     2401 caaattctat tcctttttta attactcagt ctgtcaataa tcattttatt aattatttcg
     2461 ggattcattg ttatttgtcc cttatttaaa tcagaacttt atacgatata aagaaagatt
     2521 cctgttatcg tttgttcttg tcttgctgtg tatattattt taataaccta aaccatttta
     2581 ttgtatgtcc agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga
     2641 ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc
     2701 tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat
     2761 gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt
     2821 acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt
     2881 acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt
     2941 tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca
     3001 ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga
     3061 aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca
     3121 ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa
     3181 acaacttaaa tgtgaaagtg ggtcttaatg gccacatcgg ttcctgctag aaggcccatc
     3241 ctgacggatg gccttttggt attcgagcat tacctcatac tttggatttt tgttttgttt
     3301 gcaatggtta atctattgtt aaaatttaaa gtttcacttg aactttcaaa taatgttctt
     3361 atatttgcag tgtccctatc agtgatagag agtaggatca cacaacattt aaaaaataac
     3421 attatgaaag cactcgaaaa gagattcaaa ggtaatatta acaataattt attttcaatg
     3481 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     3541 gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg agggcgatgc caccaacggc
     3601 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     3661 gtgaccacct taggctacgg cgtggcctgc ttcgcccgct accccgacca catgaagcag
     3721 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catctctttc
     3781 aaggacgacg gtacctacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     3841 aaccgcatcg tgctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     3901 ctggagtaca acttcaacag ccacaaggtc tatatcacgg ccgacaagca gaagaacggc
     3961 atcaaggcta acttcaagac ccgccacaac gttgaggacg gcggcgtgca gctcgccgac
     4021 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     4081 ctgagccatc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     4141 aaggagaggg tgaccgccgc cgggattaca catgacatgg acgagctgta caagtaatag
     4201 cgttcagctc gactgtgaga aggccatcct gacggatggc cttttttttc gttataacta
     4261 gttgcgttcc tagccgctac tagtgtaatt gcctatcttc cagtgatgga acagcatttg
     4321 tgcattggct gcaacaatca gccttacttg tgcctgttct atttccgaac cgaccgcttg
     4381 tatgaatgca tcaaaattcg ttttctctac gttggattcc ttgttgctca tattgtgatg
     4441 ataatttcta caaatatagt cattggtaac tatctatgaa actgtttgat acttttatag
     4501 ttgattaaac ttgttcatgg catttgcctt aatatcatcc gctatgtcaa tgtagggttt
     4561 catagctttg tagtcgctgt gtcccgtcca tttcatgacc acctgtgccg ggattccgag
     4621 agccagcgca ttgcagatga atgtcctttt tcctgcatgg gtactgagca aagcgtattt
     4681 gtgtgtgact tcatcaatac gttcatttcc cttgtagtag gtttcccgta caggctcgtt
     4741 gatttctgcc agttcgccca gctctttcag gtaatcgttc atcttctggt tgctgatgac
     4801 gggcagagcc atgtaattct cgaaatggat gtccttgtat ttgtccagta tggctttgct
     4861 gtatttgttc agttcaatcg tcaggctgtc ggcagtcttg actgtggtta tttcgatgtg
     4921 gtcggacttc acatcgcttc ttttcagatt gcgaacatcc gaataccgca aactcgtaaa
     4981 gcagcagaac aggaaaacat cacgcacacg ttccaggtat tgcttatcct tgggtatctg
     5041 gtagtctttc agcttgttca gttcatccca agtcaggaag attacttttt tcgaggtggt
     5101 tttcagtttc ggtttgaacg tatcgtatgc aatgttctga tgatgtcctt tcttgaagct
     5161 ccagcgcagg aaccatttga ggaatcccat ttgcttgccg atggtgctgt ttctcatatc
     5221 cttggtgtca cgcaggaagt tgacgtattc gttcaatcca aactcgttga aatagttgaa
     5281 cgttgcatcc tccttgaact ctttgaggtg gttcctcact gctgcaaatt tttcataggt
     5341 ggatgccgtc cagttattct ggttaccgca ctcttttaca aactcatcga acacctccca
     5401 aaagctgaca ggggcttctt ccggctgttc ttcactggtg tctttcattc tcatgttgaa
     5461 agcttccttc aactgttggg tcgttggcat gacctcctgc acctcaaatt ccttgaaaat
     5521 attctggatt tcggcatagt atttcagcaa gtccgtattg atttcggctg cactttgctt
     5581 tagcttgttg gtacatccgt tctttacccg ctgcttatct gcatcccatt tggctacgtc
     5641 aatccggtag cccgttgtaa actcgatacg ttggctggca aagatgacac gcatacggat
     5701 gggtacgttc tctacgattg gcacaccgtt ctttttccgg ctctccaatg caaaaatgat
     5761 gttgcgcttg atattcataa ttgggtgcgt ttgaaattct acacccaaat atacacccaa
     5821 ttattgagat agcaaaagac atttagaaac atttactttt actctatatt gtaatttaca
     5881 cttgattatc agtcgtttgc agttttatga tattctgtga aagtataagt tcgagagcct
     5941 gtctctccgc aaaaaacgct gaaaatcagc agattgcaaa acaaacaccc tgttttacac
     6001 ccaagaatgt aaagtcgggt gtttttgttt tatttaagat aatacaacca ctacataata
     6061 aaagagtagc gatattaaaa gaatccgatg agaaaagact aatatttatc tatccattca
     6121 gtttgatttc tcaggacttt acatcgtcct gaaagtattt gttggtaccg gtaccgagga
     6181 cgcgacagct gcagacgcgt tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6241 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6301 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6361 cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6421 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6481 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     6541 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6601 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6661 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6721 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6781 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6841 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6901 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6961 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7021 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7081 tcatactctt cctttttcaa tcatggccgc gggattaaaa gtcggggatt ggtgaacaaa
     7141 aaggtgtttc tctctttaag agaaatatcg ttttgctaaa cagttgatat tgaggtatca
     7201 ttttatcgta aaagacattt ttgctcaaca attgcttgac ggaaatcaac aaattttagc
     7261 attttgtaaa aaagtcgcta tataatttgg tgaattggag ttattttcat atttttgcat
     7321 cccgaagagt ttctcttaaa gagagaaaca tcttttgcat accttttccg accgaatttt
     7381 tatgtcgtaa agaggggctt tgcagggggt ggactcagaa agatgagaat agatgactat
     7441 tgtagttgaa acacatagaa agttgctgat atacagaccg atacgcatat cgggatgaac
     7501 catgagtacg ttcttttctc aaaaaacata aatattcgaa aagagatgca ataaattaag
     7561 gagaggttat aatgaacaaa gtaaatataa aagatagtca aaattttatt acttcaaaat
     7621 atcacataga aaaaataatg aattgcataa gtttagatga aaaagataac atctttgaaa
     7681 taggtgcagg gaaaggtcat tttactgctg gattggtaaa gagatgtaat tttgtaacgg
     7741 cgatagaaat tgattctaaa ttatgtgagg taactcgtaa taagctctta aattatccta
     7801 actatcaaat agtaaatgat gatatactga aatttacatt tcctagccac aatccatata
     7861 aaatatttgg caacatacct tacaacataa gcacaaatat aattcgaaaa attgtttttg
     7921 aaagttcagc cacaataagt tatttaatag tggaatatgg ttttgctaaa atgttattag
     7981 atacaaacag atcactagca ttgctgttaa tggcagaggt agatatttct atattagcaa
     8041 aaattcctag gtattatttc catccaaaac ctaaagtgga tagcacatta attgtattaa
     8101 aaagaaagcc agcaaaaatg gcatttaaag agagaaaaaa atatgaaact tttgtaatga
     8161 aatgggttaa caaagagtac gaaaaactgt ttacaaaaaa tcaatttaat aaagctttaa
     8221 aacatgcgag aatatatgat ataaacaata ttagtttcga acaatttgta tcgctattta
     8281 atagttataa aatatttaac ggctaaaaac aataggccac atgcaactgt aaatgtttac
     8341 gcgggtaccg acaccgcggt ggaggggaat tcccatgtca gccgttaagt gttcctgtgt
     8401 cactcaaaat tgctttgaga ggctctaagg gcttctcagt gcgttacatc cctggcttgt
     8461 tgtccacaac cgttaaacct taaaagcttt aaaagcctta tatattcttt tttttcttat
     8521 aaaacttaaa accttagagg ctatttaagt tgctgattta tattaatttt attgttcaaa
     8581 catgagagct tagtacgtga aacatgagag cttagtacgt tagccatgag agcttagtac
     8641 gttagccatg agggtttagt tcgttaaaca tgagagctta gtacgttaaa catgagagct
     8701 tagtacgtga aacatgagag cttagtacgt actatcaaca ggttgaactg ctgatcttca
     8761 gatcctctac gccggacgca tcgtggccgg atcaattccg ttttccgctg cataaccctg
     8821 cttcggggtc attatagcga ttttttcggt atatccatcc tttttcgcac gatatacagg
     8881 attttgccaa agggttcgtg tagactttcc ttggtgtatc caacggcgtc agccgggcag
     8941 gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca
     9001 cctggcggtg ctcaacggga atcctgctct gcgaggctgg ccggctaccg ccggcgtaac
     9061 agatgagggc aagcggatgg ctgatgaaac caagccaacc aggaagggca gcccacctat
     9121 cacggaattg atccccctcg aattggaatt ctagcctgga atagggaagc accacatcac
     9181 c
//
