LOCUS       Exported               10101 bp ds-DNA     circular SYN 17-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   giselle
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 24, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..120
                     /label=Promoter P_BfP1E6
     RBS             125..206
                     /label=RBS1P-LP
     CDS             207..896
                     /codon_start=1
                     /label=mScarlet-I3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    1023..1041
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1070..1088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1119..1137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1151..1169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1193..1211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1239..1257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1281..1299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1329..1347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1378..1396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1420..1438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1483..1501
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1530..1548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1579..1597
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1611..1629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1653..1671
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1699..1717
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1741..1759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1789..1807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1838..1856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1880..1898
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1943..1961
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1990..2008
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2039..2057
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2071..2089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2113..2131
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2159..2177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2201..2219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2249..2267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2298..2316
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2340..2358
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2403..2421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2450..2468
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2499..2517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2531..2549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2573..2591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2619..2637
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2661..2679
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2709..2727
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2758..2776
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2800..2818
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      2853..2924
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2940..2967
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        2976..3504
                     /label=PopuA
     CDS             3505..4128
                     /codon_start=1
                     /label=TetR
                     /note="TetR"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     terminator      4149..4177
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     misc_feature    4208..4315
                     /label=pTetR56
     protein_bind    4293..4311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             4316..4397
                     /label=RBS1P-LP
     CDS             4398..5117
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     terminator      5138..5165
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(5415..6698)
                     /codon_start=1
                     /transl_table=11
                     /product="NBU integrase"
                     /label=NBU integrase
                     /protein_id="ARI71345.1"
                     /translation="MNIKRNIIFALESRKKNGVPIVENVPIRMRVIFASQRIEFTTGYR
                     IDVAKWDADKQRVKNGCTNKLKQSAAEINTDLLKYYAEIQNIFKEFEVQEVMPTTQQLK
                     EAFNMRMKDTSEEQPEEAPVSFWEVFDEFVKECGNQNNWTASTYEKFAAVRNHLKEFKE
                     DATFNYFNEFGLNEYVNFLRDTKDMRNSTIGKQMGFLKWFLRWSFKKGHHQNIAYDTFK
                     PKLKTTSKKVIFLTWDELNKLKDYQIPKDKQYLERVRDVFLFCCFTSLRYSDVRNLKRS
                     DVKSDHIEITTVKTADSLTIELNKYSKAILDKYKDIHFENYMALPVISNQKMNDYLKEL
                     GELAEINEPVRETYYKGNERIDEVTHKYALLSTHAGKRTFICNALALGIPAQVVMKWTG
                     HSDYKAMKPYIDIADDIKANAMNKFNQL"
     CDS             complement(7144..8004)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /label=AmpR
                     /protein_id="ARI71346.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             8492..9226
                     /codon_start=1
                     /transl_table=11
                     /product="ErmG"
                     /label=ErmG
                     /protein_id="ARI71348.1"
                     /translation="MNKVNIKDSQNFITSKYHIEKIMNCISLDEKDNIFEIGAGKGHFT
                     AGLVKRCNFVTAIEIDSKLCEVTRNKLLNYPNYQIVNDDILKFTFPSHNPYKIFGNIPY
                     NISTNIIRKIVFESSATISYLIVEYGFAKMLLDTNRSLALLLMAEVDISILAKIPRYYF
                     HPKPKVDSTLIVLKRKPAKMAFKERKKYETFVMKWVNKEYEKLFTKNQFNKALKHARIY
                     DINNISFEQFVSLFNSYKIFNG"
     rep_origin      9296..9684
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            9855..9964
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgagc ggccgcttct agagatcgat tttagagctg
     1021 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     1081 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     1141 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     1201 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     1261 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     1321 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     1381 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     1441 taggtgtccc tagaattact agagatcgat tttagagctg cctccctatc agtgatagag
     1501 attgctacaa ctgcaaggta gtcccggtat ccctatcagt gatagagact gatagctacg
     1561 agtctgagat cctacttgtc cctatcagtg atagagacag taccgagttt tccctatcag
     1621 tgatagagag ctcatactgc gacagagtct tttccctatc agtgatagag ataggctcct
     1681 ttgtcaaaag tgatcgcttc cctatcagtg atagagactt cacgggataa tgtgttctac
     1741 tccctatcag tgatagagag cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg
     1801 atagagaaac tccgtcatcg tggatgtcaa tactgactcc ctatcagtga tagagattga
     1861 tttcaccgct ggcgtcaatt ccctatcagt gatagagact taggtgtccc tagaattact
     1921 agagatcgat tttagagctg cctccctatc agtgatagag attgctacaa ctgcaaggta
     1981 gtcccggtat ccctatcagt gatagagact gatagctacg agtctgagat cctacttgtc
     2041 cctatcagtg atagagacag taccgagttt tccctatcag tgatagagag ctcatactgc
     2101 gacagagtct tttccctatc agtgatagag ataggctcct ttgtcaaaag tgatcgcttc
     2161 cctatcagtg atagagactt cacgggataa tgtgttctac tccctatcag tgatagagag
     2221 cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg atagagaaac tccgtcatcg
     2281 tggatgtcaa tactgactcc ctatcagtga tagagattga tttcaccgct ggcgtcaatt
     2341 ccctatcagt gatagagact taggtgtccc tagaattact agagatcgat tttagagctg
     2401 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     2461 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     2521 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     2581 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     2641 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     2701 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     2761 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     2821 taggtgtccc tagaattact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     2881 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     2941 gctcaccttc gggtgggcct ttctgcgttt atatatgatg tgtaatgaca attatccttc
     3001 cccaggaaaa ttgtaatagc cctgttgaac atataaatct agtttagtta ttgacgccaa
     3061 atatacggtg tttaagtgaa atattcaaac ttattttata tttatctgcc ggtttgttct
     3121 attttgctga gtgatagggg aatattaccc ggaaactttc catatcatta caaataaagc
     3181 cctctgtatg ccccggattt tgaatataat ttgtatgttt aatccgtttt ttgtatcttt
     3241 gtgccagcaa gatactggaa caggctgttt ttatacataa aaatgctatt ttttgctatt
     3301 cgttgaaagg attatcttat caaattctat tcctttttta attactcagt ctgtcaataa
     3361 tcattttatt aattatttcg ggattcattg ttatttgtcc cttatttaaa tcagaacttt
     3421 atacgatata aagaaagatt cctgttatcg tttgttcttg tcttgctgtg tatattattt
     3481 taataaccta aaccatttta ttgtatgtcc agattagata aaagtaaagt gattaacagc
     3541 gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag
     3601 aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc
     3661 gacgccttag ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg
     3721 gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt
     3781 catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact
     3841 ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat
     3901 gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa
     3961 gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa
     4021 gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg
     4081 atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtcttaatg gccacatcgg
     4141 ttcctgctag aaggcccatc ctgacggatg gccttttggt attcgagcat tacctcatac
     4201 tttggatttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa gtttcacttg
     4261 aactttcaaa taatgttctt atatttgcag tgtccctatc agtgatagag agtaggatca
     4321 cacaacattt aaaaaataac attatgaaag cactcgaaaa gagattcaaa ggtaatatta
     4381 acaataattt attttcaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     4441 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg
     4501 agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     4561 ccgtgccctg gcccaccctc gtgaccacct taggctacgg cgtggcctgc ttcgcccgct
     4621 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4681 aggagcgcac catctctttc aaggacgacg gtacctacaa gacccgcgcc gaggtgaagt
     4741 tcgagggcga caccctggtg aaccgcatcg tgctgaaggg catcgacttc aaggaggacg
     4801 gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaaggtc tatatcacgg
     4861 ccgacaagca gaagaacggc atcaaggcta acttcaagac ccgccacaac gttgaggacg
     4921 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4981 tgctgcccga caaccactac ctgagccatc agtccaagct gagcaaagac cccaacgaga
     5041 agcgcgatca catggtcctg aaggagaggg tgaccgccgc cgggattaca catgacatgg
     5101 acgagctgta caagtaatag cgttcagctc gactgtgaga aggccatcct gacggatggc
     5161 cttttttttc gttataacta gttgcgttcc tagccgctac tagtgtaatt gcctatcttc
     5221 cagtgatgga acagcatttg tgcattggct gcaacaatca gccttacttg tgcctgttct
     5281 atttccgaac cgaccgcttg tatgaatgca tcaaaattcg ttttctctac gttggattcc
     5341 ttgttgctca tattgtgatg ataatttcta caaatatagt cattggtaac tatctatgaa
     5401 actgtttgat acttttatag ttgattaaac ttgttcatgg catttgcctt aatatcatcc
     5461 gctatgtcaa tgtagggttt catagctttg tagtcgctgt gtcccgtcca tttcatgacc
     5521 acctgtgccg ggattccgag agccagcgca ttgcagatga atgtcctttt tcctgcatgg
     5581 gtactgagca aagcgtattt gtgtgtgact tcatcaatac gttcatttcc cttgtagtag
     5641 gtttcccgta caggctcgtt gatttctgcc agttcgccca gctctttcag gtaatcgttc
     5701 atcttctggt tgctgatgac gggcagagcc atgtaattct cgaaatggat gtccttgtat
     5761 ttgtccagta tggctttgct gtatttgttc agttcaatcg tcaggctgtc ggcagtcttg
     5821 actgtggtta tttcgatgtg gtcggacttc acatcgcttc ttttcagatt gcgaacatcc
     5881 gaataccgca aactcgtaaa gcagcagaac aggaaaacat cacgcacacg ttccaggtat
     5941 tgcttatcct tgggtatctg gtagtctttc agcttgttca gttcatccca agtcaggaag
     6001 attacttttt tcgaggtggt tttcagtttc ggtttgaacg tatcgtatgc aatgttctga
     6061 tgatgtcctt tcttgaagct ccagcgcagg aaccatttga ggaatcccat ttgcttgccg
     6121 atggtgctgt ttctcatatc cttggtgtca cgcaggaagt tgacgtattc gttcaatcca
     6181 aactcgttga aatagttgaa cgttgcatcc tccttgaact ctttgaggtg gttcctcact
     6241 gctgcaaatt tttcataggt ggatgccgtc cagttattct ggttaccgca ctcttttaca
     6301 aactcatcga acacctccca aaagctgaca ggggcttctt ccggctgttc ttcactggtg
     6361 tctttcattc tcatgttgaa agcttccttc aactgttggg tcgttggcat gacctcctgc
     6421 acctcaaatt ccttgaaaat attctggatt tcggcatagt atttcagcaa gtccgtattg
     6481 atttcggctg cactttgctt tagcttgttg gtacatccgt tctttacccg ctgcttatct
     6541 gcatcccatt tggctacgtc aatccggtag cccgttgtaa actcgatacg ttggctggca
     6601 aagatgacac gcatacggat gggtacgttc tctacgattg gcacaccgtt ctttttccgg
     6661 ctctccaatg caaaaatgat gttgcgcttg atattcataa ttgggtgcgt ttgaaattct
     6721 acacccaaat atacacccaa ttattgagat agcaaaagac atttagaaac atttactttt
     6781 actctatatt gtaatttaca cttgattatc agtcgtttgc agttttatga tattctgtga
     6841 aagtataagt tcgagagcct gtctctccgc aaaaaacgct gaaaatcagc agattgcaaa
     6901 acaaacaccc tgttttacac ccaagaatgt aaagtcgggt gtttttgttt tatttaagat
     6961 aatacaacca ctacataata aaagagtagc gatattaaaa gaatccgatg agaaaagact
     7021 aatatttatc tatccattca gtttgatttc tcaggacttt acatcgtcct gaaagtattt
     7081 gttggtaccg gtaccgagga cgcgacagct gcagacgcgt tatgagtaaa cttggtctga
     7141 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7201 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7261 ccccagtgct gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat
     7321 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7381 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7441 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     7501 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7561 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     7621 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     7681 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     7741 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     7801 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7861 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7921 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7981 gacacggaaa tgttgaatac tcatactctt cctttttcaa tcatggccgc gggattaaaa
     8041 gtcggggatt ggtgaacaaa aaggtgtttc tctctttaag agaaatatcg ttttgctaaa
     8101 cagttgatat tgaggtatca ttttatcgta aaagacattt ttgctcaaca attgcttgac
     8161 ggaaatcaac aaattttagc attttgtaaa aaagtcgcta tataatttgg tgaattggag
     8221 ttattttcat atttttgcat cccgaagagt ttctcttaaa gagagaaaca tcttttgcat
     8281 accttttccg accgaatttt tatgtcgtaa agaggggctt tgcagggggt ggactcagaa
     8341 agatgagaat agatgactat tgtagttgaa acacatagaa agttgctgat atacagaccg
     8401 atacgcatat cgggatgaac catgagtacg ttcttttctc aaaaaacata aatattcgaa
     8461 aagagatgca ataaattaag gagaggttat aatgaacaaa gtaaatataa aagatagtca
     8521 aaattttatt acttcaaaat atcacataga aaaaataatg aattgcataa gtttagatga
     8581 aaaagataac atctttgaaa taggtgcagg gaaaggtcat tttactgctg gattggtaaa
     8641 gagatgtaat tttgtaacgg cgatagaaat tgattctaaa ttatgtgagg taactcgtaa
     8701 taagctctta aattatccta actatcaaat agtaaatgat gatatactga aatttacatt
     8761 tcctagccac aatccatata aaatatttgg caacatacct tacaacataa gcacaaatat
     8821 aattcgaaaa attgtttttg aaagttcagc cacaataagt tatttaatag tggaatatgg
     8881 ttttgctaaa atgttattag atacaaacag atcactagca ttgctgttaa tggcagaggt
     8941 agatatttct atattagcaa aaattcctag gtattatttc catccaaaac ctaaagtgga
     9001 tagcacatta attgtattaa aaagaaagcc agcaaaaatg gcatttaaag agagaaaaaa
     9061 atatgaaact tttgtaatga aatgggttaa caaagagtac gaaaaactgt ttacaaaaaa
     9121 tcaatttaat aaagctttaa aacatgcgag aatatatgat ataaacaata ttagtttcga
     9181 acaatttgta tcgctattta atagttataa aatatttaac ggctaaaaac aataggccac
     9241 atgcaactgt aaatgtttac gcgggtaccg acaccgcggt ggaggggaat tcccatgtca
     9301 gccgttaagt gttcctgtgt cactcaaaat tgctttgaga ggctctaagg gcttctcagt
     9361 gcgttacatc cctggcttgt tgtccacaac cgttaaacct taaaagcttt aaaagcctta
     9421 tatattcttt tttttcttat aaaacttaaa accttagagg ctatttaagt tgctgattta
     9481 tattaatttt attgttcaaa catgagagct tagtacgtga aacatgagag cttagtacgt
     9541 tagccatgag agcttagtac gttagccatg agggtttagt tcgttaaaca tgagagctta
     9601 gtacgttaaa catgagagct tagtacgtga aacatgagag cttagtacgt actatcaaca
     9661 ggttgaactg ctgatcttca gatcctctac gccggacgca tcgtggccgg atcaattccg
     9721 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc
     9781 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc
     9841 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc
     9901 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg
     9961 ccggctaccg ccggcgtaac agatgagggc aagcggatgg ctgatgaaac caagccaacc
    10021 aggaagggca gcccacctat cacggaattg atccccctcg aattggaatt ctagcctgga
    10081 atagggaagc accacatcac c
//
