LOCUS       Exported                8681 bp ds-DNA     circular SYN 17-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   giselle
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 24, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..120
                     /label=Promoter P_BfP1E6
     regulatory      125..206
                     /regulatory_class="ribosome_binding_site"
                     /label=RBS1p-LP
     CDS             207..896
                     /codon_start=1
                     /label=mScarlet-I3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    1023..1041
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1070..1088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1119..1137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1151..1169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1193..1211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1239..1257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1281..1299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1329..1347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1378..1396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1420..1438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      1473..1544
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1560..1587
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        1600..2084
                     /label=PBT0779
     CDS             2085..2708
                     /codon_start=1
                     /label=TetR
                     /note="TetR"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     terminator      2729..2757
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     misc_feature    2788..2895
                     /label=pTetR56
     protein_bind    2873..2891
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2896..2977
                     /label=RBS1P-LP
     CDS             2978..3697
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     terminator      3718..3745
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(3995..5278)
                     /codon_start=1
                     /transl_table=11
                     /product="NBU integrase"
                     /label=NBU integrase
                     /protein_id="ARI71345.1"
                     /translation="MNIKRNIIFALESRKKNGVPIVENVPIRMRVIFASQRIEFTTGYR
                     IDVAKWDADKQRVKNGCTNKLKQSAAEINTDLLKYYAEIQNIFKEFEVQEVMPTTQQLK
                     EAFNMRMKDTSEEQPEEAPVSFWEVFDEFVKECGNQNNWTASTYEKFAAVRNHLKEFKE
                     DATFNYFNEFGLNEYVNFLRDTKDMRNSTIGKQMGFLKWFLRWSFKKGHHQNIAYDTFK
                     PKLKTTSKKVIFLTWDELNKLKDYQIPKDKQYLERVRDVFLFCCFTSLRYSDVRNLKRS
                     DVKSDHIEITTVKTADSLTIELNKYSKAILDKYKDIHFENYMALPVISNQKMNDYLKEL
                     GELAEINEPVRETYYKGNERIDEVTHKYALLSTHAGKRTFICNALALGIPAQVVMKWTG
                     HSDYKAMKPYIDIADDIKANAMNKFNQL"
     CDS             complement(5724..6584)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /label=AmpR
                     /protein_id="ARI71346.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             7072..7806
                     /codon_start=1
                     /transl_table=11
                     /product="ErmG"
                     /label=ErmG
                     /protein_id="ARI71348.1"
                     /translation="MNKVNIKDSQNFITSKYHIEKIMNCISLDEKDNIFEIGAGKGHFT
                     AGLVKRCNFVTAIEIDSKLCEVTRNKLLNYPNYQIVNDDILKFTFPSHNPYKIFGNIPY
                     NISTNIIRKIVFESSATISYLIVEYGFAKMLLDTNRSLALLLMAEVDISILAKIPRYYF
                     HPKPKVDSTLIVLKRKPAKMAFKERKKYETFVMKWVNKEYEKLFTKNQFNKALKHARIY
                     DINNISFEQFVSLFNSYKIFNG"
     rep_origin      7876..8264
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            8435..8544
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgagc ggccgcttct agagatcgat tttagagctg
     1021 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     1081 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     1141 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     1201 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     1261 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     1321 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     1381 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     1441 taggtgtccc tagaattact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1501 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1561 gctcaccttc gggtgggcct ttctgcgttt atatatgatg gcagcatcca ttgcctgaaa
     1621 aataaccaga accgagtagc ggtcttgtgc ataaagcata ctttgcagtt ctgacagctt
     1681 ttcgatattt tgggctaact gttctttggc atcctgtttg gacatatcag cagtaaatga
     1741 gggattgaaa tcggatacat gatgttcctt ccccggtttg gctgtaagct cttttaagac
     1801 ttcttttttc attgttgtat aattaggttg ttataggtat ataacagttg aataaagaaa
     1861 tcagttcttg ccaaattaag agcgttgaat agtaattccc caattatgta tcaacagatt
     1921 ctacataatt ccacactaat tccacaatta tatttatatt gtctgctgtt tatatgtttg
     1981 attatcagtt atatatggcg gagtgaaacg tttggcatct gctttgctat atattaagta
     2041 caagaaacaa tagttctatt attcacactt aaaaaattac gattatgtcc agattagata
     2101 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa
     2161 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa
     2221 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc
     2281 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt
     2341 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta
     2401 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
     2461 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat
     2521 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
     2581 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct
     2641 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg
     2701 ggtcttaatg gccacatcgg ttcctgctag aaggcccatc ctgacggatg gccttttggt
     2761 attcgagcat tacctcatac tttggatttt tgttttgttt gcaatggtta atctattgtt
     2821 aaaatttaaa gtttcacttg aactttcaaa taatgttctt atatttgcag tgtccctatc
     2881 agtgatagag agtaggatca cacaacattt aaaaaataac attatgaaag cactcgaaaa
     2941 gagattcaaa ggtaatatta acaataattt attttcaatg gtgagcaagg gcgaggagct
     3001 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3061 cagcgtccgc ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat
     3121 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct taggctacgg
     3181 cgtggcctgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     3241 catgcccgaa ggctacgtcc aggagcgcac catctctttc aaggacgacg gtacctacaa
     3301 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg tgctgaaggg
     3361 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag
     3421 ccacaaggtc tatatcacgg ccgacaagca gaagaacggc atcaaggcta acttcaagac
     3481 ccgccacaac gttgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
     3541 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagccatc agtccaagct
     3601 gagcaaagac cccaacgaga agcgcgatca catggtcctg aaggagaggg tgaccgccgc
     3661 cgggattaca catgacatgg acgagctgta caagtaatag cgttcagctc gactgtgaga
     3721 aggccatcct gacggatggc cttttttttc gttataacta gttgcgttcc tagccgctac
     3781 tagtgtaatt gcctatcttc cagtgatgga acagcatttg tgcattggct gcaacaatca
     3841 gccttacttg tgcctgttct atttccgaac cgaccgcttg tatgaatgca tcaaaattcg
     3901 ttttctctac gttggattcc ttgttgctca tattgtgatg ataatttcta caaatatagt
     3961 cattggtaac tatctatgaa actgtttgat acttttatag ttgattaaac ttgttcatgg
     4021 catttgcctt aatatcatcc gctatgtcaa tgtagggttt catagctttg tagtcgctgt
     4081 gtcccgtcca tttcatgacc acctgtgccg ggattccgag agccagcgca ttgcagatga
     4141 atgtcctttt tcctgcatgg gtactgagca aagcgtattt gtgtgtgact tcatcaatac
     4201 gttcatttcc cttgtagtag gtttcccgta caggctcgtt gatttctgcc agttcgccca
     4261 gctctttcag gtaatcgttc atcttctggt tgctgatgac gggcagagcc atgtaattct
     4321 cgaaatggat gtccttgtat ttgtccagta tggctttgct gtatttgttc agttcaatcg
     4381 tcaggctgtc ggcagtcttg actgtggtta tttcgatgtg gtcggacttc acatcgcttc
     4441 ttttcagatt gcgaacatcc gaataccgca aactcgtaaa gcagcagaac aggaaaacat
     4501 cacgcacacg ttccaggtat tgcttatcct tgggtatctg gtagtctttc agcttgttca
     4561 gttcatccca agtcaggaag attacttttt tcgaggtggt tttcagtttc ggtttgaacg
     4621 tatcgtatgc aatgttctga tgatgtcctt tcttgaagct ccagcgcagg aaccatttga
     4681 ggaatcccat ttgcttgccg atggtgctgt ttctcatatc cttggtgtca cgcaggaagt
     4741 tgacgtattc gttcaatcca aactcgttga aatagttgaa cgttgcatcc tccttgaact
     4801 ctttgaggtg gttcctcact gctgcaaatt tttcataggt ggatgccgtc cagttattct
     4861 ggttaccgca ctcttttaca aactcatcga acacctccca aaagctgaca ggggcttctt
     4921 ccggctgttc ttcactggtg tctttcattc tcatgttgaa agcttccttc aactgttggg
     4981 tcgttggcat gacctcctgc acctcaaatt ccttgaaaat attctggatt tcggcatagt
     5041 atttcagcaa gtccgtattg atttcggctg cactttgctt tagcttgttg gtacatccgt
     5101 tctttacccg ctgcttatct gcatcccatt tggctacgtc aatccggtag cccgttgtaa
     5161 actcgatacg ttggctggca aagatgacac gcatacggat gggtacgttc tctacgattg
     5221 gcacaccgtt ctttttccgg ctctccaatg caaaaatgat gttgcgcttg atattcataa
     5281 ttgggtgcgt ttgaaattct acacccaaat atacacccaa ttattgagat agcaaaagac
     5341 atttagaaac atttactttt actctatatt gtaatttaca cttgattatc agtcgtttgc
     5401 agttttatga tattctgtga aagtataagt tcgagagcct gtctctccgc aaaaaacgct
     5461 gaaaatcagc agattgcaaa acaaacaccc tgttttacac ccaagaatgt aaagtcgggt
     5521 gtttttgttt tatttaagat aatacaacca ctacataata aaagagtagc gatattaaaa
     5581 gaatccgatg agaaaagact aatatttatc tatccattca gtttgatttc tcaggacttt
     5641 acatcgtcct gaaagtattt gttggtaccg gtaccgagga cgcgacagct gcagacgcgt
     5701 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     5761 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     5821 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgggaccc acgctcaccg
     5881 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     5941 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6001 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6061 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6121 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6181 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6241 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6301 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6361 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     6421 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     6481 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     6541 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     6601 tcatggccgc gggattaaaa gtcggggatt ggtgaacaaa aaggtgtttc tctctttaag
     6661 agaaatatcg ttttgctaaa cagttgatat tgaggtatca ttttatcgta aaagacattt
     6721 ttgctcaaca attgcttgac ggaaatcaac aaattttagc attttgtaaa aaagtcgcta
     6781 tataatttgg tgaattggag ttattttcat atttttgcat cccgaagagt ttctcttaaa
     6841 gagagaaaca tcttttgcat accttttccg accgaatttt tatgtcgtaa agaggggctt
     6901 tgcagggggt ggactcagaa agatgagaat agatgactat tgtagttgaa acacatagaa
     6961 agttgctgat atacagaccg atacgcatat cgggatgaac catgagtacg ttcttttctc
     7021 aaaaaacata aatattcgaa aagagatgca ataaattaag gagaggttat aatgaacaaa
     7081 gtaaatataa aagatagtca aaattttatt acttcaaaat atcacataga aaaaataatg
     7141 aattgcataa gtttagatga aaaagataac atctttgaaa taggtgcagg gaaaggtcat
     7201 tttactgctg gattggtaaa gagatgtaat tttgtaacgg cgatagaaat tgattctaaa
     7261 ttatgtgagg taactcgtaa taagctctta aattatccta actatcaaat agtaaatgat
     7321 gatatactga aatttacatt tcctagccac aatccatata aaatatttgg caacatacct
     7381 tacaacataa gcacaaatat aattcgaaaa attgtttttg aaagttcagc cacaataagt
     7441 tatttaatag tggaatatgg ttttgctaaa atgttattag atacaaacag atcactagca
     7501 ttgctgttaa tggcagaggt agatatttct atattagcaa aaattcctag gtattatttc
     7561 catccaaaac ctaaagtgga tagcacatta attgtattaa aaagaaagcc agcaaaaatg
     7621 gcatttaaag agagaaaaaa atatgaaact tttgtaatga aatgggttaa caaagagtac
     7681 gaaaaactgt ttacaaaaaa tcaatttaat aaagctttaa aacatgcgag aatatatgat
     7741 ataaacaata ttagtttcga acaatttgta tcgctattta atagttataa aatatttaac
     7801 ggctaaaaac aataggccac atgcaactgt aaatgtttac gcgggtaccg acaccgcggt
     7861 ggaggggaat tcccatgtca gccgttaagt gttcctgtgt cactcaaaat tgctttgaga
     7921 ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac cgttaaacct
     7981 taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa accttagagg
     8041 ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct tagtacgtga
     8101 aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg agggtttagt
     8161 tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga aacatgagag
     8221 cttagtacgt actatcaaca ggttgaactg ctgatcttca gatcctctac gccggacgca
     8281 tcgtggccgg atcaattccg ttttccgctg cataaccctg cttcggggtc attatagcga
     8341 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg
     8401 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac
     8461 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga
     8521 atcctgctct gcgaggctgg ccggctaccg ccggcgtaac agatgagggc aagcggatgg
     8581 ctgatgaaac caagccaacc aggaagggca gcccacctat cacggaattg atccccctcg
     8641 aattggaatt ctagcctgga atagggaagc accacatcac c
//
