LOCUS       Exported               10061 bp ds-DNA     circular SYN 17-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   giselle
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 24, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..120
                     /label=Promoter P_BfP1E6
     RBS             125..206
                     /label=RBS1P-LP
     CDS             207..896
                     /codon_start=1
                     /label=mScarlet-I3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    1023..1041
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1070..1088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1119..1137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1151..1169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1193..1211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1239..1257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1281..1299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1329..1347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1378..1396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1420..1438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1483..1501
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1530..1548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1579..1597
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1611..1629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1653..1671
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1699..1717
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1741..1759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1789..1807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1838..1856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1880..1898
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1943..1961
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1990..2008
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2039..2057
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2071..2089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2113..2131
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2159..2177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2201..2219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2249..2267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2298..2316
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2340..2358
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2403..2421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2450..2468
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2499..2517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2531..2549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2573..2591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2619..2637
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2661..2679
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2709..2727
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2758..2776
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2800..2818
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      2853..2924
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2940..2967
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        2980..3464
                     /label=PBT0779
     CDS             3465..4088
                     /codon_start=1
                     /label=TetR
                     /note="TetR"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     terminator      4109..4137
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     misc_feature    4168..4275
                     /label=pTetR56
     protein_bind    4253..4271
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             4276..4357
                     /label=RBS1P-LP
     CDS             4358..5077
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     terminator      5098..5125
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(5375..6658)
                     /codon_start=1
                     /transl_table=11
                     /product="NBU integrase"
                     /label=NBU integrase
                     /protein_id="ARI71345.1"
                     /translation="MNIKRNIIFALESRKKNGVPIVENVPIRMRVIFASQRIEFTTGYR
                     IDVAKWDADKQRVKNGCTNKLKQSAAEINTDLLKYYAEIQNIFKEFEVQEVMPTTQQLK
                     EAFNMRMKDTSEEQPEEAPVSFWEVFDEFVKECGNQNNWTASTYEKFAAVRNHLKEFKE
                     DATFNYFNEFGLNEYVNFLRDTKDMRNSTIGKQMGFLKWFLRWSFKKGHHQNIAYDTFK
                     PKLKTTSKKVIFLTWDELNKLKDYQIPKDKQYLERVRDVFLFCCFTSLRYSDVRNLKRS
                     DVKSDHIEITTVKTADSLTIELNKYSKAILDKYKDIHFENYMALPVISNQKMNDYLKEL
                     GELAEINEPVRETYYKGNERIDEVTHKYALLSTHAGKRTFICNALALGIPAQVVMKWTG
                     HSDYKAMKPYIDIADDIKANAMNKFNQL"
     CDS             complement(7104..7964)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /label=AmpR
                     /protein_id="ARI71346.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             8452..9186
                     /codon_start=1
                     /transl_table=11
                     /product="ErmG"
                     /label=ErmG
                     /protein_id="ARI71348.1"
                     /translation="MNKVNIKDSQNFITSKYHIEKIMNCISLDEKDNIFEIGAGKGHFT
                     AGLVKRCNFVTAIEIDSKLCEVTRNKLLNYPNYQIVNDDILKFTFPSHNPYKIFGNIPY
                     NISTNIIRKIVFESSATISYLIVEYGFAKMLLDTNRSLALLLMAEVDISILAKIPRYYF
                     HPKPKVDSTLIVLKRKPAKMAFKERKKYETFVMKWVNKEYEKLFTKNQFNKALKHARIY
                     DINNISFEQFVSLFNSYKIFNG"
     rep_origin      9256..9644
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            9815..9924
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgagc ggccgcttct agagatcgat tttagagctg
     1021 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     1081 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     1141 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     1201 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     1261 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     1321 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     1381 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     1441 taggtgtccc tagaattact agagatcgat tttagagctg cctccctatc agtgatagag
     1501 attgctacaa ctgcaaggta gtcccggtat ccctatcagt gatagagact gatagctacg
     1561 agtctgagat cctacttgtc cctatcagtg atagagacag taccgagttt tccctatcag
     1621 tgatagagag ctcatactgc gacagagtct tttccctatc agtgatagag ataggctcct
     1681 ttgtcaaaag tgatcgcttc cctatcagtg atagagactt cacgggataa tgtgttctac
     1741 tccctatcag tgatagagag cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg
     1801 atagagaaac tccgtcatcg tggatgtcaa tactgactcc ctatcagtga tagagattga
     1861 tttcaccgct ggcgtcaatt ccctatcagt gatagagact taggtgtccc tagaattact
     1921 agagatcgat tttagagctg cctccctatc agtgatagag attgctacaa ctgcaaggta
     1981 gtcccggtat ccctatcagt gatagagact gatagctacg agtctgagat cctacttgtc
     2041 cctatcagtg atagagacag taccgagttt tccctatcag tgatagagag ctcatactgc
     2101 gacagagtct tttccctatc agtgatagag ataggctcct ttgtcaaaag tgatcgcttc
     2161 cctatcagtg atagagactt cacgggataa tgtgttctac tccctatcag tgatagagag
     2221 cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg atagagaaac tccgtcatcg
     2281 tggatgtcaa tactgactcc ctatcagtga tagagattga tttcaccgct ggcgtcaatt
     2341 ccctatcagt gatagagact taggtgtccc tagaattact agagatcgat tttagagctg
     2401 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     2461 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     2521 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     2581 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     2641 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     2701 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     2761 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     2821 taggtgtccc tagaattact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     2881 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     2941 gctcaccttc gggtgggcct ttctgcgttt atatatgatg gcagcatcca ttgcctgaaa
     3001 aataaccaga accgagtagc ggtcttgtgc ataaagcata ctttgcagtt ctgacagctt
     3061 ttcgatattt tgggctaact gttctttggc atcctgtttg gacatatcag cagtaaatga
     3121 gggattgaaa tcggatacat gatgttcctt ccccggtttg gctgtaagct cttttaagac
     3181 ttcttttttc attgttgtat aattaggttg ttataggtat ataacagttg aataaagaaa
     3241 tcagttcttg ccaaattaag agcgttgaat agtaattccc caattatgta tcaacagatt
     3301 ctacataatt ccacactaat tccacaatta tatttatatt gtctgctgtt tatatgtttg
     3361 attatcagtt atatatggcg gagtgaaacg tttggcatct gctttgctat atattaagta
     3421 caagaaacaa tagttctatt attcacactt aaaaaattac gattatgtcc agattagata
     3481 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa
     3541 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa
     3601 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc
     3661 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt
     3721 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta
     3781 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
     3841 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat
     3901 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
     3961 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct
     4021 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg
     4081 ggtcttaatg gccacatcgg ttcctgctag aaggcccatc ctgacggatg gccttttggt
     4141 attcgagcat tacctcatac tttggatttt tgttttgttt gcaatggtta atctattgtt
     4201 aaaatttaaa gtttcacttg aactttcaaa taatgttctt atatttgcag tgtccctatc
     4261 agtgatagag agtaggatca cacaacattt aaaaaataac attatgaaag cactcgaaaa
     4321 gagattcaaa ggtaatatta acaataattt attttcaatg gtgagcaagg gcgaggagct
     4381 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     4441 cagcgtccgc ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat
     4501 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct taggctacgg
     4561 cgtggcctgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     4621 catgcccgaa ggctacgtcc aggagcgcac catctctttc aaggacgacg gtacctacaa
     4681 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg tgctgaaggg
     4741 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag
     4801 ccacaaggtc tatatcacgg ccgacaagca gaagaacggc atcaaggcta acttcaagac
     4861 ccgccacaac gttgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc
     4921 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagccatc agtccaagct
     4981 gagcaaagac cccaacgaga agcgcgatca catggtcctg aaggagaggg tgaccgccgc
     5041 cgggattaca catgacatgg acgagctgta caagtaatag cgttcagctc gactgtgaga
     5101 aggccatcct gacggatggc cttttttttc gttataacta gttgcgttcc tagccgctac
     5161 tagtgtaatt gcctatcttc cagtgatgga acagcatttg tgcattggct gcaacaatca
     5221 gccttacttg tgcctgttct atttccgaac cgaccgcttg tatgaatgca tcaaaattcg
     5281 ttttctctac gttggattcc ttgttgctca tattgtgatg ataatttcta caaatatagt
     5341 cattggtaac tatctatgaa actgtttgat acttttatag ttgattaaac ttgttcatgg
     5401 catttgcctt aatatcatcc gctatgtcaa tgtagggttt catagctttg tagtcgctgt
     5461 gtcccgtcca tttcatgacc acctgtgccg ggattccgag agccagcgca ttgcagatga
     5521 atgtcctttt tcctgcatgg gtactgagca aagcgtattt gtgtgtgact tcatcaatac
     5581 gttcatttcc cttgtagtag gtttcccgta caggctcgtt gatttctgcc agttcgccca
     5641 gctctttcag gtaatcgttc atcttctggt tgctgatgac gggcagagcc atgtaattct
     5701 cgaaatggat gtccttgtat ttgtccagta tggctttgct gtatttgttc agttcaatcg
     5761 tcaggctgtc ggcagtcttg actgtggtta tttcgatgtg gtcggacttc acatcgcttc
     5821 ttttcagatt gcgaacatcc gaataccgca aactcgtaaa gcagcagaac aggaaaacat
     5881 cacgcacacg ttccaggtat tgcttatcct tgggtatctg gtagtctttc agcttgttca
     5941 gttcatccca agtcaggaag attacttttt tcgaggtggt tttcagtttc ggtttgaacg
     6001 tatcgtatgc aatgttctga tgatgtcctt tcttgaagct ccagcgcagg aaccatttga
     6061 ggaatcccat ttgcttgccg atggtgctgt ttctcatatc cttggtgtca cgcaggaagt
     6121 tgacgtattc gttcaatcca aactcgttga aatagttgaa cgttgcatcc tccttgaact
     6181 ctttgaggtg gttcctcact gctgcaaatt tttcataggt ggatgccgtc cagttattct
     6241 ggttaccgca ctcttttaca aactcatcga acacctccca aaagctgaca ggggcttctt
     6301 ccggctgttc ttcactggtg tctttcattc tcatgttgaa agcttccttc aactgttggg
     6361 tcgttggcat gacctcctgc acctcaaatt ccttgaaaat attctggatt tcggcatagt
     6421 atttcagcaa gtccgtattg atttcggctg cactttgctt tagcttgttg gtacatccgt
     6481 tctttacccg ctgcttatct gcatcccatt tggctacgtc aatccggtag cccgttgtaa
     6541 actcgatacg ttggctggca aagatgacac gcatacggat gggtacgttc tctacgattg
     6601 gcacaccgtt ctttttccgg ctctccaatg caaaaatgat gttgcgcttg atattcataa
     6661 ttgggtgcgt ttgaaattct acacccaaat atacacccaa ttattgagat agcaaaagac
     6721 atttagaaac atttactttt actctatatt gtaatttaca cttgattatc agtcgtttgc
     6781 agttttatga tattctgtga aagtataagt tcgagagcct gtctctccgc aaaaaacgct
     6841 gaaaatcagc agattgcaaa acaaacaccc tgttttacac ccaagaatgt aaagtcgggt
     6901 gtttttgttt tatttaagat aatacaacca ctacataata aaagagtagc gatattaaaa
     6961 gaatccgatg agaaaagact aatatttatc tatccattca gtttgatttc tcaggacttt
     7021 acatcgtcct gaaagtattt gttggtaccg gtaccgagga cgcgacagct gcagacgcgt
     7081 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7141 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7201 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgggaccc acgctcaccg
     7261 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7321 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7381 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7441 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7501 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7561 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7621 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7681 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7741 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7801 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7861 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7921 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7981 tcatggccgc gggattaaaa gtcggggatt ggtgaacaaa aaggtgtttc tctctttaag
     8041 agaaatatcg ttttgctaaa cagttgatat tgaggtatca ttttatcgta aaagacattt
     8101 ttgctcaaca attgcttgac ggaaatcaac aaattttagc attttgtaaa aaagtcgcta
     8161 tataatttgg tgaattggag ttattttcat atttttgcat cccgaagagt ttctcttaaa
     8221 gagagaaaca tcttttgcat accttttccg accgaatttt tatgtcgtaa agaggggctt
     8281 tgcagggggt ggactcagaa agatgagaat agatgactat tgtagttgaa acacatagaa
     8341 agttgctgat atacagaccg atacgcatat cgggatgaac catgagtacg ttcttttctc
     8401 aaaaaacata aatattcgaa aagagatgca ataaattaag gagaggttat aatgaacaaa
     8461 gtaaatataa aagatagtca aaattttatt acttcaaaat atcacataga aaaaataatg
     8521 aattgcataa gtttagatga aaaagataac atctttgaaa taggtgcagg gaaaggtcat
     8581 tttactgctg gattggtaaa gagatgtaat tttgtaacgg cgatagaaat tgattctaaa
     8641 ttatgtgagg taactcgtaa taagctctta aattatccta actatcaaat agtaaatgat
     8701 gatatactga aatttacatt tcctagccac aatccatata aaatatttgg caacatacct
     8761 tacaacataa gcacaaatat aattcgaaaa attgtttttg aaagttcagc cacaataagt
     8821 tatttaatag tggaatatgg ttttgctaaa atgttattag atacaaacag atcactagca
     8881 ttgctgttaa tggcagaggt agatatttct atattagcaa aaattcctag gtattatttc
     8941 catccaaaac ctaaagtgga tagcacatta attgtattaa aaagaaagcc agcaaaaatg
     9001 gcatttaaag agagaaaaaa atatgaaact tttgtaatga aatgggttaa caaagagtac
     9061 gaaaaactgt ttacaaaaaa tcaatttaat aaagctttaa aacatgcgag aatatatgat
     9121 ataaacaata ttagtttcga acaatttgta tcgctattta atagttataa aatatttaac
     9181 ggctaaaaac aataggccac atgcaactgt aaatgtttac gcgggtaccg acaccgcggt
     9241 ggaggggaat tcccatgtca gccgttaagt gttcctgtgt cactcaaaat tgctttgaga
     9301 ggctctaagg gcttctcagt gcgttacatc cctggcttgt tgtccacaac cgttaaacct
     9361 taaaagcttt aaaagcctta tatattcttt tttttcttat aaaacttaaa accttagagg
     9421 ctatttaagt tgctgattta tattaatttt attgttcaaa catgagagct tagtacgtga
     9481 aacatgagag cttagtacgt tagccatgag agcttagtac gttagccatg agggtttagt
     9541 tcgttaaaca tgagagctta gtacgttaaa catgagagct tagtacgtga aacatgagag
     9601 cttagtacgt actatcaaca ggttgaactg ctgatcttca gatcctctac gccggacgca
     9661 tcgtggccgg atcaattccg ttttccgctg cataaccctg cttcggggtc attatagcga
     9721 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg
     9781 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac
     9841 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga
     9901 atcctgctct gcgaggctgg ccggctaccg ccggcgtaac agatgagggc aagcggatgg
     9961 ctgatgaaac caagccaacc aggaagggca gcccacctat cacggaattg atccccctcg
    10021 aattggaatt ctagcctgga atagggaagc accacatcac c
//
