LOCUS       Exported               11901 bp ds-DNA     circular SYN 17-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11901)
  AUTHORS   giselle
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 24, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11901
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..120
                     /label=Promoter P_BfP1E6
     RBS             125..206
                     /label=RBS1P-LP
     CDS             207..896
                     /codon_start=1
                     /label=mScarlet-I3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYWKQSFPEGFKWERVMIFEDGGT
                     VSVTQDTSLEDGTLIYKVKLRGGNFPPDGPVMQKRTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     terminator      919..946
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    1023..1041
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1070..1088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1119..1137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1151..1169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1193..1211
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1239..1257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1281..1299
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1329..1347
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1378..1396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1420..1438
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1483..1501
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1530..1548
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1579..1597
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1611..1629
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1653..1671
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1699..1717
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1741..1759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1789..1807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1838..1856
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1880..1898
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1943..1961
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1990..2008
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2039..2057
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2071..2089
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2113..2131
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2159..2177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2201..2219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2249..2267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2298..2316
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2340..2358
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2403..2421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2450..2468
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2499..2517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2531..2549
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2573..2591
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2619..2637
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2661..2679
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2709..2727
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2758..2776
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2800..2818
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2863..2881
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2910..2928
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2959..2977
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2991..3009
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3033..3051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3079..3097
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3121..3139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3169..3187
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3218..3236
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3260..3278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3323..3341
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3370..3388
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3419..3437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3451..3469
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3493..3511
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3539..3557
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3581..3599
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3629..3647
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3678..3696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3720..3738
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3783..3801
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3830..3848
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3879..3897
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3911..3929
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3953..3971
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3999..4017
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4041..4059
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4089..4107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4138..4156
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4180..4198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4243..4261
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4290..4308
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4339..4357
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4371..4389
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4413..4431
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4459..4477
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4501..4519
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4549..4567
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4598..4616
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4640..4658
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      4693..4764
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4780..4807
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        4820..5304
                     /label=PBT0779
     CDS             5305..5928
                     /codon_start=1
                     /label=TetR
                     /note="TetR"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     terminator      5949..5977
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     misc_feature    6008..6115
                     /label=pTetR56
     protein_bind    6093..6111
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      6116..6197
                     /regulatory_class="ribosome_binding_site"
                     /label=RBS1p-LP
     CDS             6198..6917
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     terminator      6938..6965
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(7215..8498)
                     /codon_start=1
                     /transl_table=11
                     /product="NBU integrase"
                     /label=NBU integrase
                     /protein_id="ARI71345.1"
                     /translation="MNIKRNIIFALESRKKNGVPIVENVPIRMRVIFASQRIEFTTGYR
                     IDVAKWDADKQRVKNGCTNKLKQSAAEINTDLLKYYAEIQNIFKEFEVQEVMPTTQQLK
                     EAFNMRMKDTSEEQPEEAPVSFWEVFDEFVKECGNQNNWTASTYEKFAAVRNHLKEFKE
                     DATFNYFNEFGLNEYVNFLRDTKDMRNSTIGKQMGFLKWFLRWSFKKGHHQNIAYDTFK
                     PKLKTTSKKVIFLTWDELNKLKDYQIPKDKQYLERVRDVFLFCCFTSLRYSDVRNLKRS
                     DVKSDHIEITTVKTADSLTIELNKYSKAILDKYKDIHFENYMALPVISNQKMNDYLKEL
                     GELAEINEPVRETYYKGNERIDEVTHKYALLSTHAGKRTFICNALALGIPAQVVMKWTG
                     HSDYKAMKPYIDIADDIKANAMNKFNQL"
     CDS             complement(8944..9804)
                     /codon_start=1
                     /transl_table=11
                     /product="AmpR"
                     /label=AmpR
                     /protein_id="ARI71346.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             10292..11026
                     /codon_start=1
                     /transl_table=11
                     /product="ErmG"
                     /label=ErmG
                     /protein_id="ARI71348.1"
                     /translation="MNKVNIKDSQNFITSKYHIEKIMNCISLDEKDNIFEIGAGKGHFT
                     AGLVKRCNFVTAIEIDSKLCEVTRNKLLNYPNYQIVNDDILKFTFPSHNPYKIFGNIPY
                     NISTNIIRKIVFESSATISYLIVEYGFAKMLLDTNRSLALLLMAEVDISILAKIPRYYF
                     HPKPKVDSTLIVLKRKPAKMAFKERKKYETFVMKWVNKEYEKLFTKNQFNKALKHARIY
                     DINNISFEQFVSLFNSYKIFNG"
     rep_origin      11096..11484
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            11655..11764
                     /note="incP origin of transfer"
ORIGIN
        1 caattgggct accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa
       61 gtttcacttg aactttcaaa taatgttctt atatttgcag tgtcgaaaga aacaaagtag
      121 gactgatcac acaacattta aaaaataaca ttatgaaagc actcgaaaag agattcaaag
      181 gtaatattaa caataattta ttttcaatgg atagcaccga ggcagtgatc aaggagttca
      241 tgcggttcaa ggtgcacatg gagggctcca tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      361 gccccctgcc cttctcctgg gacatcctgt cccctcagtt catgtacggc tccagggcct
      421 tcatcaagca ccccgccgac atccccgact actggaagca gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgatc ttcgaggacg gcggcaccgt gtccgtgacc caggacacct
      541 ccctggagga cggcaccctg atctacaagg tgaagctccg cggcggcaac ttccctcctg
      601 acggccccgt aatgcagaag aggacaatgg gctgggaagc atccaccgag cggttgtacc
      661 ccgaggacgt cgtgctgaag ggcgacatta agatggccct gcgcctgaag gacggcggcc
      721 gctacctggc ggacttcaag accacctaca aggccaagaa gcccgtgcag atgcccggcg
      781 ccttcaacat cgaccgcaag ttggacatca cctcccacaa cgaggactac accgtggtgg
      841 aacagtacga acgctccgtg gcccgccact ccaccggcgg ctccggtggc tcctaataaa
      901 tcctagctga ttagctcgag aaggccatcc tgacggatgg cctttttttt gtatggggac
      961 tctgagaacc ctacctttca aaccccgagc ggccgcttct agagatcgat tttagagctg
     1021 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     1081 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     1141 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     1201 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     1261 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     1321 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     1381 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     1441 taggtgtccc tagaattact agagatcgat tttagagctg cctccctatc agtgatagag
     1501 attgctacaa ctgcaaggta gtcccggtat ccctatcagt gatagagact gatagctacg
     1561 agtctgagat cctacttgtc cctatcagtg atagagacag taccgagttt tccctatcag
     1621 tgatagagag ctcatactgc gacagagtct tttccctatc agtgatagag ataggctcct
     1681 ttgtcaaaag tgatcgcttc cctatcagtg atagagactt cacgggataa tgtgttctac
     1741 tccctatcag tgatagagag cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg
     1801 atagagaaac tccgtcatcg tggatgtcaa tactgactcc ctatcagtga tagagattga
     1861 tttcaccgct ggcgtcaatt ccctatcagt gatagagact taggtgtccc tagaattact
     1921 agagatcgat tttagagctg cctccctatc agtgatagag attgctacaa ctgcaaggta
     1981 gtcccggtat ccctatcagt gatagagact gatagctacg agtctgagat cctacttgtc
     2041 cctatcagtg atagagacag taccgagttt tccctatcag tgatagagag ctcatactgc
     2101 gacagagtct tttccctatc agtgatagag ataggctcct ttgtcaaaag tgatcgcttc
     2161 cctatcagtg atagagactt cacgggataa tgtgttctac tccctatcag tgatagagag
     2221 cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg atagagaaac tccgtcatcg
     2281 tggatgtcaa tactgactcc ctatcagtga tagagattga tttcaccgct ggcgtcaatt
     2341 ccctatcagt gatagagact taggtgtccc tagaattact agagatcgat tttagagctg
     2401 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     2461 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     2521 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     2581 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     2641 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     2701 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     2761 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     2821 taggtgtccc tagaattact agagatcgat tttagagctg cctccctatc agtgatagag
     2881 attgctacaa ctgcaaggta gtcccggtat ccctatcagt gatagagact gatagctacg
     2941 agtctgagat cctacttgtc cctatcagtg atagagacag taccgagttt tccctatcag
     3001 tgatagagag ctcatactgc gacagagtct tttccctatc agtgatagag ataggctcct
     3061 ttgtcaaaag tgatcgcttc cctatcagtg atagagactt cacgggataa tgtgttctac
     3121 tccctatcag tgatagagag cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg
     3181 atagagaaac tccgtcatcg tggatgtcaa tactgactcc ctatcagtga tagagattga
     3241 tttcaccgct ggcgtcaatt ccctatcagt gatagagact taggtgtccc tagaattact
     3301 agagatcgat tttagagctg cctccctatc agtgatagag attgctacaa ctgcaaggta
     3361 gtcccggtat ccctatcagt gatagagact gatagctacg agtctgagat cctacttgtc
     3421 cctatcagtg atagagacag taccgagttt tccctatcag tgatagagag ctcatactgc
     3481 gacagagtct tttccctatc agtgatagag ataggctcct ttgtcaaaag tgatcgcttc
     3541 cctatcagtg atagagactt cacgggataa tgtgttctac tccctatcag tgatagagag
     3601 cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg atagagaaac tccgtcatcg
     3661 tggatgtcaa tactgactcc ctatcagtga tagagattga tttcaccgct ggcgtcaatt
     3721 ccctatcagt gatagagact taggtgtccc tagaattact agagatcgat tttagagctg
     3781 cctccctatc agtgatagag attgctacaa ctgcaaggta gtcccggtat ccctatcagt
     3841 gatagagact gatagctacg agtctgagat cctacttgtc cctatcagtg atagagacag
     3901 taccgagttt tccctatcag tgatagagag ctcatactgc gacagagtct tttccctatc
     3961 agtgatagag ataggctcct ttgtcaaaag tgatcgcttc cctatcagtg atagagactt
     4021 cacgggataa tgtgttctac tccctatcag tgatagagag cttaagtcaa tccaatgcgt
     4081 tcaggtgctc cctatcagtg atagagaaac tccgtcatcg tggatgtcaa tactgactcc
     4141 ctatcagtga tagagattga tttcaccgct ggcgtcaatt ccctatcagt gatagagact
     4201 taggtgtccc tagaattact agagatcgat tttagagctg cctccctatc agtgatagag
     4261 attgctacaa ctgcaaggta gtcccggtat ccctatcagt gatagagact gatagctacg
     4321 agtctgagat cctacttgtc cctatcagtg atagagacag taccgagttt tccctatcag
     4381 tgatagagag ctcatactgc gacagagtct tttccctatc agtgatagag ataggctcct
     4441 ttgtcaaaag tgatcgcttc cctatcagtg atagagactt cacgggataa tgtgttctac
     4501 tccctatcag tgatagagag cttaagtcaa tccaatgcgt tcaggtgctc cctatcagtg
     4561 atagagaaac tccgtcatcg tggatgtcaa tactgactcc ctatcagtga tagagattga
     4621 tttcaccgct ggcgtcaatt ccctatcagt gatagagact taggtgtccc tagaattact
     4681 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     4741 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     4801 ttctgcgttt atatatgatg gcagcatcca ttgcctgaaa aataaccaga accgagtagc
     4861 ggtcttgtgc ataaagcata ctttgcagtt ctgacagctt ttcgatattt tgggctaact
     4921 gttctttggc atcctgtttg gacatatcag cagtaaatga gggattgaaa tcggatacat
     4981 gatgttcctt ccccggtttg gctgtaagct cttttaagac ttcttttttc attgttgtat
     5041 aattaggttg ttataggtat ataacagttg aataaagaaa tcagttcttg ccaaattaag
     5101 agcgttgaat agtaattccc caattatgta tcaacagatt ctacataatt ccacactaat
     5161 tccacaatta tatttatatt gtctgctgtt tatatgtttg attatcagtt atatatggcg
     5221 gagtgaaacg tttggcatct gctttgctat atattaagta caagaaacaa tagttctatt
     5281 attcacactt aaaaaattac gattatgtcc agattagata aaagtaaagt gattaacagc
     5341 gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag
     5401 aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc
     5461 gacgccttag ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg
     5521 gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt
     5581 catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact
     5641 ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat
     5701 gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa
     5761 gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa
     5821 gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg
     5881 atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtcttaatg gccacatcgg
     5941 ttcctgctag aaggcccatc ctgacggatg gccttttggt attcgagcat tacctcatac
     6001 tttggatttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa gtttcacttg
     6061 aactttcaaa taatgttctt atatttgcag tgtccctatc agtgatagag agtaggatca
     6121 cacaacattt aaaaaataac attatgaaag cactcgaaaa gagattcaaa ggtaatatta
     6181 acaataattt attttcaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     6241 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg
     6301 agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     6361 ccgtgccctg gcccaccctc gtgaccacct taggctacgg cgtggcctgc ttcgcccgct
     6421 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     6481 aggagcgcac catctctttc aaggacgacg gtacctacaa gacccgcgcc gaggtgaagt
     6541 tcgagggcga caccctggtg aaccgcatcg tgctgaaggg catcgacttc aaggaggacg
     6601 gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaaggtc tatatcacgg
     6661 ccgacaagca gaagaacggc atcaaggcta acttcaagac ccgccacaac gttgaggacg
     6721 gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     6781 tgctgcccga caaccactac ctgagccatc agtccaagct gagcaaagac cccaacgaga
     6841 agcgcgatca catggtcctg aaggagaggg tgaccgccgc cgggattaca catgacatgg
     6901 acgagctgta caagtaatag cgttcagctc gactgtgaga aggccatcct gacggatggc
     6961 cttttttttc gttataacta gttgcgttcc tagccgctac tagtgtaatt gcctatcttc
     7021 cagtgatgga acagcatttg tgcattggct gcaacaatca gccttacttg tgcctgttct
     7081 atttccgaac cgaccgcttg tatgaatgca tcaaaattcg ttttctctac gttggattcc
     7141 ttgttgctca tattgtgatg ataatttcta caaatatagt cattggtaac tatctatgaa
     7201 actgtttgat acttttatag ttgattaaac ttgttcatgg catttgcctt aatatcatcc
     7261 gctatgtcaa tgtagggttt catagctttg tagtcgctgt gtcccgtcca tttcatgacc
     7321 acctgtgccg ggattccgag agccagcgca ttgcagatga atgtcctttt tcctgcatgg
     7381 gtactgagca aagcgtattt gtgtgtgact tcatcaatac gttcatttcc cttgtagtag
     7441 gtttcccgta caggctcgtt gatttctgcc agttcgccca gctctttcag gtaatcgttc
     7501 atcttctggt tgctgatgac gggcagagcc atgtaattct cgaaatggat gtccttgtat
     7561 ttgtccagta tggctttgct gtatttgttc agttcaatcg tcaggctgtc ggcagtcttg
     7621 actgtggtta tttcgatgtg gtcggacttc acatcgcttc ttttcagatt gcgaacatcc
     7681 gaataccgca aactcgtaaa gcagcagaac aggaaaacat cacgcacacg ttccaggtat
     7741 tgcttatcct tgggtatctg gtagtctttc agcttgttca gttcatccca agtcaggaag
     7801 attacttttt tcgaggtggt tttcagtttc ggtttgaacg tatcgtatgc aatgttctga
     7861 tgatgtcctt tcttgaagct ccagcgcagg aaccatttga ggaatcccat ttgcttgccg
     7921 atggtgctgt ttctcatatc cttggtgtca cgcaggaagt tgacgtattc gttcaatcca
     7981 aactcgttga aatagttgaa cgttgcatcc tccttgaact ctttgaggtg gttcctcact
     8041 gctgcaaatt tttcataggt ggatgccgtc cagttattct ggttaccgca ctcttttaca
     8101 aactcatcga acacctccca aaagctgaca ggggcttctt ccggctgttc ttcactggtg
     8161 tctttcattc tcatgttgaa agcttccttc aactgttggg tcgttggcat gacctcctgc
     8221 acctcaaatt ccttgaaaat attctggatt tcggcatagt atttcagcaa gtccgtattg
     8281 atttcggctg cactttgctt tagcttgttg gtacatccgt tctttacccg ctgcttatct
     8341 gcatcccatt tggctacgtc aatccggtag cccgttgtaa actcgatacg ttggctggca
     8401 aagatgacac gcatacggat gggtacgttc tctacgattg gcacaccgtt ctttttccgg
     8461 ctctccaatg caaaaatgat gttgcgcttg atattcataa ttgggtgcgt ttgaaattct
     8521 acacccaaat atacacccaa ttattgagat agcaaaagac atttagaaac atttactttt
     8581 actctatatt gtaatttaca cttgattatc agtcgtttgc agttttatga tattctgtga
     8641 aagtataagt tcgagagcct gtctctccgc aaaaaacgct gaaaatcagc agattgcaaa
     8701 acaaacaccc tgttttacac ccaagaatgt aaagtcgggt gtttttgttt tatttaagat
     8761 aatacaacca ctacataata aaagagtagc gatattaaaa gaatccgatg agaaaagact
     8821 aatatttatc tatccattca gtttgatttc tcaggacttt acatcgtcct gaaagtattt
     8881 gttggtaccg gtaccgagga cgcgacagct gcagacgcgt tatgagtaaa cttggtctga
     8941 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9001 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9061 ccccagtgct gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat
     9121 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9181 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9241 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9301 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9361 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9421 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9481 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9541 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9601 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9661 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9721 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9781 gacacggaaa tgttgaatac tcatactctt cctttttcaa tcatggccgc gggattaaaa
     9841 gtcggggatt ggtgaacaaa aaggtgtttc tctctttaag agaaatatcg ttttgctaaa
     9901 cagttgatat tgaggtatca ttttatcgta aaagacattt ttgctcaaca attgcttgac
     9961 ggaaatcaac aaattttagc attttgtaaa aaagtcgcta tataatttgg tgaattggag
    10021 ttattttcat atttttgcat cccgaagagt ttctcttaaa gagagaaaca tcttttgcat
    10081 accttttccg accgaatttt tatgtcgtaa agaggggctt tgcagggggt ggactcagaa
    10141 agatgagaat agatgactat tgtagttgaa acacatagaa agttgctgat atacagaccg
    10201 atacgcatat cgggatgaac catgagtacg ttcttttctc aaaaaacata aatattcgaa
    10261 aagagatgca ataaattaag gagaggttat aatgaacaaa gtaaatataa aagatagtca
    10321 aaattttatt acttcaaaat atcacataga aaaaataatg aattgcataa gtttagatga
    10381 aaaagataac atctttgaaa taggtgcagg gaaaggtcat tttactgctg gattggtaaa
    10441 gagatgtaat tttgtaacgg cgatagaaat tgattctaaa ttatgtgagg taactcgtaa
    10501 taagctctta aattatccta actatcaaat agtaaatgat gatatactga aatttacatt
    10561 tcctagccac aatccatata aaatatttgg caacatacct tacaacataa gcacaaatat
    10621 aattcgaaaa attgtttttg aaagttcagc cacaataagt tatttaatag tggaatatgg
    10681 ttttgctaaa atgttattag atacaaacag atcactagca ttgctgttaa tggcagaggt
    10741 agatatttct atattagcaa aaattcctag gtattatttc catccaaaac ctaaagtgga
    10801 tagcacatta attgtattaa aaagaaagcc agcaaaaatg gcatttaaag agagaaaaaa
    10861 atatgaaact tttgtaatga aatgggttaa caaagagtac gaaaaactgt ttacaaaaaa
    10921 tcaatttaat aaagctttaa aacatgcgag aatatatgat ataaacaata ttagtttcga
    10981 acaatttgta tcgctattta atagttataa aatatttaac ggctaaaaac aataggccac
    11041 atgcaactgt aaatgtttac gcgggtaccg acaccgcggt ggaggggaat tcccatgtca
    11101 gccgttaagt gttcctgtgt cactcaaaat tgctttgaga ggctctaagg gcttctcagt
    11161 gcgttacatc cctggcttgt tgtccacaac cgttaaacct taaaagcttt aaaagcctta
    11221 tatattcttt tttttcttat aaaacttaaa accttagagg ctatttaagt tgctgattta
    11281 tattaatttt attgttcaaa catgagagct tagtacgtga aacatgagag cttagtacgt
    11341 tagccatgag agcttagtac gttagccatg agggtttagt tcgttaaaca tgagagctta
    11401 gtacgttaaa catgagagct tagtacgtga aacatgagag cttagtacgt actatcaaca
    11461 ggttgaactg ctgatcttca gatcctctac gccggacgca tcgtggccgg atcaattccg
    11521 ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt atatccatcc
    11581 tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc ttggtgtatc
    11641 caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg gtgttccttc
    11701 ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct gcgaggctgg
    11761 ccggctaccg ccggcgtaac agatgagggc aagcggatgg ctgatgaaac caagccaacc
    11821 aggaagggca gcccacctat cacggaattg atccccctcg aattggaatt ctagcctgga
    11881 atagggaagc accacatcac c
//
