LOCUS       Exported                3168 bp ds-DNA     circular SYN 17-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   giselle
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 24, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        34..197
                     /label=PBfP2E5
     RBS             202..283
                     /label=RBS1P-LP
     CDS             284..907
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESET"
     terminator      1029..1115
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1361..1949)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      2132..2226
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2247..2906)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      3090..3133
                     /note="bacterial terminator"
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gttcctcgat tcagatctaa aggatccaat ctagataaaa cgaaaggctc agtcgaaaga
       61 ctgggccttt cgttttacaa ttgggctacc ttttttttgt tttgtttgca atggttaatc
      121 tattgttaaa atttaaagtt tcacttgaac tttcaaataa tgttcttata tttccagtgt
      181 cgaaagaaac aaagtaggac tgatcacaca acatttaaaa aataacatta tgaaagcact
      241 cgaaaagaga ttcaaaggta atattaacaa taatttattt tcaatgtcca gattagataa
      301 aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac
      361 aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa
      421 aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc accatactca
      481 cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt
      541 tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac
      601 agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc
      661 actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt
      721 ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat
      781 gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt
      841 cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtga
      901 gacgtaagtg gagtgacctt aaatcaagga cgatattaat cggaaggagt attcaacgtg
      961 atgaagtcgc agggttgacg tgggacgtct ctcggtataa cgccggaacc gtcccccgct
     1021 agaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1081 gtttgtcggt gaacgctctc ctgagtagga caaatgcgga caataaagga tcgtcagaag
     1141 gtaacctgtt atacaacggt gagaccttac ttcgcgttat gcaggcttcc tcgctcactg
     1201 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     1261 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     1321 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc
     1381 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1441 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1501 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1561 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1621 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1681 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1741 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1801 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1861 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1921 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1981 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2041 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2101 agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac gcccggcggc
     2161 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     2221 ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt
     2281 tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga
     2341 atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg
     2401 ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag
     2461 ggattggctg acacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt
     2521 tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg
     2581 tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg
     2641 tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga
     2701 gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc
     2761 tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga
     2821 gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg
     2881 gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac
     2941 tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg
     3001 tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     3061 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
     3121 taaggatgat ttctggaatt cgcggccgct tctagaatct aggtctcc
//
