LOCUS       Exported                9851 bp ds-DNA     circular SYN 17-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   giselle
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 24, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9851
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    86..104
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    135..153
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    167..185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    209..227
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    255..273
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    297..315
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    345..363
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    394..412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    436..454
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    499..517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    546..564
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    595..613
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    627..645
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    669..687
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    715..733
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    757..775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    805..823
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    854..872
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    896..914
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    959..977
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1006..1024
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1055..1073
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1087..1105
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1129..1147
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1175..1193
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1217..1235
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1265..1283
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1314..1332
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1356..1374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1419..1437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1466..1484
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1515..1533
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1547..1565
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1589..1607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1635..1653
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1677..1695
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1725..1743
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1774..1792
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1816..1834
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1879..1897
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1926..1944
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1975..1993
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2007..2025
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2049..2067
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2095..2113
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2137..2155
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2185..2203
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2234..2252
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2276..2294
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2339..2357
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2386..2404
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2435..2453
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2467..2485
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2509..2527
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2555..2573
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2597..2615
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2645..2663
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2694..2712
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2736..2754
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2799..2817
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2846..2864
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2895..2913
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2927..2945
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2969..2987
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3015..3033
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3057..3075
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3105..3123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3154..3172
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3196..3214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3259..3277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3306..3324
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3355..3373
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3387..3405
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3429..3447
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3475..3493
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3517..3535
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3565..3583
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3614..3632
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3656..3674
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3719..3737
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3766..3784
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3815..3833
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3847..3865
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3889..3907
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3935..3953
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3977..3995
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4025..4043
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4074..4092
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4116..4134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4179..4197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4226..4244
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4275..4293
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4307..4325
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4349..4367
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4395..4413
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4437..4455
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4485..4503
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4534..4552
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4576..4594
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4639..4657
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4686..4704
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4735..4753
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4767..4785
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4809..4827
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4855..4873
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4897..4915
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4945..4963
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4994..5012
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5036..5054
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5099..5117
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5146..5164
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5195..5213
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5227..5245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5269..5287
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5315..5333
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5357..5375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5405..5423
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5454..5472
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5496..5514
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5559..5577
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5606..5624
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5655..5673
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5687..5705
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5729..5747
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5775..5793
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5817..5835
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5865..5883
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5914..5932
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5956..5974
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6019..6037
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6066..6084
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6115..6133
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6147..6165
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6189..6207
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6235..6253
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6277..6295
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6325..6343
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6374..6392
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6416..6434
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6479..6497
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6526..6544
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6575..6593
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6607..6625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6649..6667
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6695..6713
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6737..6755
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6785..6803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6834..6852
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6876..6894
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6939..6957
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6986..7004
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7035..7053
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7067..7085
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7109..7127
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7155..7173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7197..7215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7245..7263
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7294..7312
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7336..7354
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      7389..7460
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      7476..7503
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(7591..8406)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      complement(9001..9545)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
ORIGIN
        1 ccgagcggcc gcttctagag atcgatttta gagctgcctc cctatcagtg atagagattg
       61 ctacaactgc aaggtagtcc cggtatccct atcagtgata gagactgata gctacgagtc
      121 tgagatccta cttgtcccta tcagtgatag agacagtacc gagttttccc tatcagtgat
      181 agagagctca tactgcgaca gagtcttttc cctatcagtg atagagatag gctcctttgt
      241 caaaagtgat cgcttcccta tcagtgatag agacttcacg ggataatgtg ttctactccc
      301 tatcagtgat agagagctta agtcaatcca atgcgttcag gtgctcccta tcagtgatag
      361 agaaactccg tcatcgtgga tgtcaatact gactccctat cagtgataga gattgatttc
      421 accgctggcg tcaattccct atcagtgata gagacttagg tgtccctaga attactagag
      481 atcgatttta gagctgcctc cctatcagtg atagagattg ctacaactgc aaggtagtcc
      541 cggtatccct atcagtgata gagactgata gctacgagtc tgagatccta cttgtcccta
      601 tcagtgatag agacagtacc gagttttccc tatcagtgat agagagctca tactgcgaca
      661 gagtcttttc cctatcagtg atagagatag gctcctttgt caaaagtgat cgcttcccta
      721 tcagtgatag agacttcacg ggataatgtg ttctactccc tatcagtgat agagagctta
      781 agtcaatcca atgcgttcag gtgctcccta tcagtgatag agaaactccg tcatcgtgga
      841 tgtcaatact gactccctat cagtgataga gattgatttc accgctggcg tcaattccct
      901 atcagtgata gagacttagg tgtccctaga attactagag atcgatttta gagctgcctc
      961 cctatcagtg atagagattg ctacaactgc aaggtagtcc cggtatccct atcagtgata
     1021 gagactgata gctacgagtc tgagatccta cttgtcccta tcagtgatag agacagtacc
     1081 gagttttccc tatcagtgat agagagctca tactgcgaca gagtcttttc cctatcagtg
     1141 atagagatag gctcctttgt caaaagtgat cgcttcccta tcagtgatag agacttcacg
     1201 ggataatgtg ttctactccc tatcagtgat agagagctta agtcaatcca atgcgttcag
     1261 gtgctcccta tcagtgatag agaaactccg tcatcgtgga tgtcaatact gactccctat
     1321 cagtgataga gattgatttc accgctggcg tcaattccct atcagtgata gagacttagg
     1381 tgtccctaga attactagag atcgatttta gagctgcctc cctatcagtg atagagattg
     1441 ctacaactgc aaggtagtcc cggtatccct atcagtgata gagactgata gctacgagtc
     1501 tgagatccta cttgtcccta tcagtgatag agacagtacc gagttttccc tatcagtgat
     1561 agagagctca tactgcgaca gagtcttttc cctatcagtg atagagatag gctcctttgt
     1621 caaaagtgat cgcttcccta tcagtgatag agacttcacg ggataatgtg ttctactccc
     1681 tatcagtgat agagagctta agtcaatcca atgcgttcag gtgctcccta tcagtgatag
     1741 agaaactccg tcatcgtgga tgtcaatact gactccctat cagtgataga gattgatttc
     1801 accgctggcg tcaattccct atcagtgata gagacttagg tgtccctaga attactagag
     1861 atcgatttta gagctgcctc cctatcagtg atagagattg ctacaactgc aaggtagtcc
     1921 cggtatccct atcagtgata gagactgata gctacgagtc tgagatccta cttgtcccta
     1981 tcagtgatag agacagtacc gagttttccc tatcagtgat agagagctca tactgcgaca
     2041 gagtcttttc cctatcagtg atagagatag gctcctttgt caaaagtgat cgcttcccta
     2101 tcagtgatag agacttcacg ggataatgtg ttctactccc tatcagtgat agagagctta
     2161 agtcaatcca atgcgttcag gtgctcccta tcagtgatag agaaactccg tcatcgtgga
     2221 tgtcaatact gactccctat cagtgataga gattgatttc accgctggcg tcaattccct
     2281 atcagtgata gagacttagg tgtccctaga attactagag atcgatttta gagctgcctc
     2341 cctatcagtg atagagattg ctacaactgc aaggtagtcc cggtatccct atcagtgata
     2401 gagactgata gctacgagtc tgagatccta cttgtcccta tcagtgatag agacagtacc
     2461 gagttttccc tatcagtgat agagagctca tactgcgaca gagtcttttc cctatcagtg
     2521 atagagatag gctcctttgt caaaagtgat cgcttcccta tcagtgatag agacttcacg
     2581 ggataatgtg ttctactccc tatcagtgat agagagctta agtcaatcca atgcgttcag
     2641 gtgctcccta tcagtgatag agaaactccg tcatcgtgga tgtcaatact gactccctat
     2701 cagtgataga gattgatttc accgctggcg tcaattccct atcagtgata gagacttagg
     2761 tgtccctaga attactagag atcgatttta gagctgcctc cctatcagtg atagagattg
     2821 ctacaactgc aaggtagtcc cggtatccct atcagtgata gagactgata gctacgagtc
     2881 tgagatccta cttgtcccta tcagtgatag agacagtacc gagttttccc tatcagtgat
     2941 agagagctca tactgcgaca gagtcttttc cctatcagtg atagagatag gctcctttgt
     3001 caaaagtgat cgcttcccta tcagtgatag agacttcacg ggataatgtg ttctactccc
     3061 tatcagtgat agagagctta agtcaatcca atgcgttcag gtgctcccta tcagtgatag
     3121 agaaactccg tcatcgtgga tgtcaatact gactccctat cagtgataga gattgatttc
     3181 accgctggcg tcaattccct atcagtgata gagacttagg tgtccctaga attactagag
     3241 atcgatttta gagctgcctc cctatcagtg atagagattg ctacaactgc aaggtagtcc
     3301 cggtatccct atcagtgata gagactgata gctacgagtc tgagatccta cttgtcccta
     3361 tcagtgatag agacagtacc gagttttccc tatcagtgat agagagctca tactgcgaca
     3421 gagtcttttc cctatcagtg atagagatag gctcctttgt caaaagtgat cgcttcccta
     3481 tcagtgatag agacttcacg ggataatgtg ttctactccc tatcagtgat agagagctta
     3541 agtcaatcca atgcgttcag gtgctcccta tcagtgatag agaaactccg tcatcgtgga
     3601 tgtcaatact gactccctat cagtgataga gattgatttc accgctggcg tcaattccct
     3661 atcagtgata gagacttagg tgtccctaga attactagag atcgatttta gagctgcctc
     3721 cctatcagtg atagagattg ctacaactgc aaggtagtcc cggtatccct atcagtgata
     3781 gagactgata gctacgagtc tgagatccta cttgtcccta tcagtgatag agacagtacc
     3841 gagttttccc tatcagtgat agagagctca tactgcgaca gagtcttttc cctatcagtg
     3901 atagagatag gctcctttgt caaaagtgat cgcttcccta tcagtgatag agacttcacg
     3961 ggataatgtg ttctactccc tatcagtgat agagagctta agtcaatcca atgcgttcag
     4021 gtgctcccta tcagtgatag agaaactccg tcatcgtgga tgtcaatact gactccctat
     4081 cagtgataga gattgatttc accgctggcg tcaattccct atcagtgata gagacttagg
     4141 tgtccctaga attactagag atcgatttta gagctgcctc cctatcagtg atagagattg
     4201 ctacaactgc aaggtagtcc cggtatccct atcagtgata gagactgata gctacgagtc
     4261 tgagatccta cttgtcccta tcagtgatag agacagtacc gagttttccc tatcagtgat
     4321 agagagctca tactgcgaca gagtcttttc cctatcagtg atagagatag gctcctttgt
     4381 caaaagtgat cgcttcccta tcagtgatag agacttcacg ggataatgtg ttctactccc
     4441 tatcagtgat agagagctta agtcaatcca atgcgttcag gtgctcccta tcagtgatag
     4501 agaaactccg tcatcgtgga tgtcaatact gactccctat cagtgataga gattgatttc
     4561 accgctggcg tcaattccct atcagtgata gagacttagg tgtccctaga attactagag
     4621 atcgatttta gagctgcctc cctatcagtg atagagattg ctacaactgc aaggtagtcc
     4681 cggtatccct atcagtgata gagactgata gctacgagtc tgagatccta cttgtcccta
     4741 tcagtgatag agacagtacc gagttttccc tatcagtgat agagagctca tactgcgaca
     4801 gagtcttttc cctatcagtg atagagatag gctcctttgt caaaagtgat cgcttcccta
     4861 tcagtgatag agacttcacg ggataatgtg ttctactccc tatcagtgat agagagctta
     4921 agtcaatcca atgcgttcag gtgctcccta tcagtgatag agaaactccg tcatcgtgga
     4981 tgtcaatact gactccctat cagtgataga gattgatttc accgctggcg tcaattccct
     5041 atcagtgata gagacttagg tgtccctaga attactagag atcgatttta gagctgcctc
     5101 cctatcagtg atagagattg ctacaactgc aaggtagtcc cggtatccct atcagtgata
     5161 gagactgata gctacgagtc tgagatccta cttgtcccta tcagtgatag agacagtacc
     5221 gagttttccc tatcagtgat agagagctca tactgcgaca gagtcttttc cctatcagtg
     5281 atagagatag gctcctttgt caaaagtgat cgcttcccta tcagtgatag agacttcacg
     5341 ggataatgtg ttctactccc tatcagtgat agagagctta agtcaatcca atgcgttcag
     5401 gtgctcccta tcagtgatag agaaactccg tcatcgtgga tgtcaatact gactccctat
     5461 cagtgataga gattgatttc accgctggcg tcaattccct atcagtgata gagacttagg
     5521 tgtccctaga attactagag atcgatttta gagctgcctc cctatcagtg atagagattg
     5581 ctacaactgc aaggtagtcc cggtatccct atcagtgata gagactgata gctacgagtc
     5641 tgagatccta cttgtcccta tcagtgatag agacagtacc gagttttccc tatcagtgat
     5701 agagagctca tactgcgaca gagtcttttc cctatcagtg atagagatag gctcctttgt
     5761 caaaagtgat cgcttcccta tcagtgatag agacttcacg ggataatgtg ttctactccc
     5821 tatcagtgat agagagctta agtcaatcca atgcgttcag gtgctcccta tcagtgatag
     5881 agaaactccg tcatcgtgga tgtcaatact gactccctat cagtgataga gattgatttc
     5941 accgctggcg tcaattccct atcagtgata gagacttagg tgtccctaga attactagag
     6001 atcgatttta gagctgcctc cctatcagtg atagagattg ctacaactgc aaggtagtcc
     6061 cggtatccct atcagtgata gagactgata gctacgagtc tgagatccta cttgtcccta
     6121 tcagtgatag agacagtacc gagttttccc tatcagtgat agagagctca tactgcgaca
     6181 gagtcttttc cctatcagtg atagagatag gctcctttgt caaaagtgat cgcttcccta
     6241 tcagtgatag agacttcacg ggataatgtg ttctactccc tatcagtgat agagagctta
     6301 agtcaatcca atgcgttcag gtgctcccta tcagtgatag agaaactccg tcatcgtgga
     6361 tgtcaatact gactccctat cagtgataga gattgatttc accgctggcg tcaattccct
     6421 atcagtgata gagacttagg tgtccctaga attactagag atcgatttta gagctgcctc
     6481 cctatcagtg atagagattg ctacaactgc aaggtagtcc cggtatccct atcagtgata
     6541 gagactgata gctacgagtc tgagatccta cttgtcccta tcagtgatag agacagtacc
     6601 gagttttccc tatcagtgat agagagctca tactgcgaca gagtcttttc cctatcagtg
     6661 atagagatag gctcctttgt caaaagtgat cgcttcccta tcagtgatag agacttcacg
     6721 ggataatgtg ttctactccc tatcagtgat agagagctta agtcaatcca atgcgttcag
     6781 gtgctcccta tcagtgatag agaaactccg tcatcgtgga tgtcaatact gactccctat
     6841 cagtgataga gattgatttc accgctggcg tcaattccct atcagtgata gagacttagg
     6901 tgtccctaga attactagag atcgatttta gagctgcctc cctatcagtg atagagattg
     6961 ctacaactgc aaggtagtcc cggtatccct atcagtgata gagactgata gctacgagtc
     7021 tgagatccta cttgtcccta tcagtgatag agacagtacc gagttttccc tatcagtgat
     7081 agagagctca tactgcgaca gagtcttttc cctatcagtg atagagatag gctcctttgt
     7141 caaaagtgat cgcttcccta tcagtgatag agacttcacg ggataatgtg ttctactccc
     7201 tatcagtgat agagagctta agtcaatcca atgcgttcag gtgctcccta tcagtgatag
     7261 agaaactccg tcatcgtgga tgtcaatact gactccctat cagtgataga gattgatttc
     7321 accgctggcg tcaattccct atcagtgata gagacttagg tgtccctaga attactagag
     7381 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     7441 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     7501 gcgtttatat atgatcgaga ccggtaatct cgagtcccgt caagtcagcg taatgctctg
     7561 ccagtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa
     7621 ctgcaattta ttcatatcag gattatcgat accatatttt tgaaaaagcc gtttctgtaa
     7681 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     7741 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     7801 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg
     7861 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     7921 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     7981 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     8041 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc
     8101 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     8161 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     8221 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     8281 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     8341 atcagcatcc atgttggaat ttaatcgcgg cctggagcaa gacgtttccc gttgaatatg
     8401 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga
     8461 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg
     8521 aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga
     8581 ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct
     8641 catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga
     8701 gtcagcaaca ccttcttcac gaggcagacc tcagcgctag cggagtgtat actggcttac
     8761 tatgttggca ctgatgaggg tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg
     8821 caccggtgcg tcagcagaat atgtgataca ggatatattc cgcttcctcg ctcactgact
     8881 cgctacgctc ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt
     8941 tcctggaaga tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt
     9001 tttccatagg ctccgccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg
     9061 cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc cctcgtgcgc
     9121 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct
     9181 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatgca
     9241 cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9301 cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat ttagaggagt
     9361 tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt ggtgactgcg
     9421 ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc ttcaaaaaac
     9481 cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga ccaaaacgat
     9541 ctcaagaaga tcatcttatt aaggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9601 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9661 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9721 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9781 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9841 tgatggtctc g
//
