LOCUS       JL605_px601fix\C        5377 bp    DNA     circular SYN 08-FEB-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Ye L Wang J Tan Y Beyer AI Xie F Muench MO Kan YW
  TITLE     Genome editing using CRISPR-Cas9 to create the HPFH genotype in 
            HSPCs: An approach for treating sickle cell disease and 
            beta-thalassemia.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10661-5. doi: 
            10.1073/pnas.1612075113. Epub 2016 Sep 6.
  PUBMED    27601644
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jun 20, 2017 from SnapGene Console 4.0.0a8 
            http://www.snapgene.com
REFERENCE   3  (bases 1 to 5377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 26, 2025 from SnapGene 8.2.0
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1612075113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-09-20- 20"; volume: "113"; issue: "38"; pages: "10661-5"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Exported 
            Tuesday, Jun 20, 2017 from SnapGene Console 4.0.0a8 
            http://www.snapgene.com"
COMMENT     Imported From
            Path: C:\Users\jonlevy\Downloads
            Filename: addgene-plasmid-84040-sequence-164139.gbk
FEATURES             Location/Qualifiers
     source          1..5377
                     /lab_host="synthetic DNA construct"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          4869..5117
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:1476495"
                     /organism="Cloning vector pAAV-CW3SL-EGFP"
     repeat_region   complement(23..106)
                     /label=3'-ITR pSub201(+)
                     /note="/modified by=jonlevy"
                     /note="/vntifkey=34"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     complement(23..34)
                     /label=QB0039 L4440
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     primer_bind     84..95
                     /label=QB0039 L4440
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     misc_feature    122..282
                     /label=lacZ_a reporter
                     /note="Geneious type: reporter"
                     /note="/Imported from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Plas
                     Mapper</a>"
                     /note="/Transferred From=lacZ_a reporter"
                     /note="/Transferred Similarity=100.00%"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(125..142)
                     /label=Eton M13F(-21)
                     /note="color: black; sequence: TGTAAAACGACGGCCAGT"
     primer_bind     complement(125..142)
                     /label=Genewiz M13F(-21)
                     /note="color: black; sequence: TGTAAAACGACGGCCAGT"
     primer_bind     complement(125..142)
                     /label=QB0046 M13 (-21) Forward
                     /note="color: black; sequence: TGTAAAACGACGGCCAGT"
     primer_bind     complement(125..141)
                     /label=M13-F (-20)
                     /note="color: black; sequence: GTAAAACGACGGCCAGT"
     primer_bind     complement(134..156)
                     /label=QB0049 M13/pUC Forward
                     /note="color: black; sequence: CCCAGTCACGACGTTGTAAAACG"
     primer_bind     complement(145..168)
                     /label=Genewiz M13F(-47)
                     /note="color: black; sequence: CGCCAGGGTTTTCCCAGTCACGAC"
     primer_bind     complement(145..162)
                     /label=Genewiz M13F(-41)
                     /note="color: black; sequence: GGTTTTCCCAGTCACGAC"
     primer_bind     complement(145..161)
                     /label=Eton M13F(-40)
                     /note="color: black; sequence: GTTTTCCCAGTCACGAC"
     primer_bind     complement(145..161)
                     /label=M13-F (-40)
                     /note="color: black; sequence: GTTTTCCCAGTCACGAC"
     primer_bind     complement(145..161)
                     /label=QB0047 M13 (-40)
                     /note="color: black; sequence: GTTTTCCCAGTCACGAC"
     primer_bind     complement(146..167)
                     /label=M13-F (-46)
                     /note="color: black; sequence: GCCAGGGTTTTCCCAGTCACGA"
     primer_bind     complement(209..228)
                     /label=LacZ-R (EtonSeq)
                     /note="color: black; sequence: CTTCGCTATTACGCCAGCTG"
     primer_bind     209..228
                     /label=834_LacZ-fwd1
                     /note="color: black; sequence: CAGCTGGCGTAATAGCGAAG"
     primer_bind     complement(247..268)
                     /label=NMG-236
                     /note="color: black; sequence: AGGCTGCGCAACTGTTGGGAAG"
     misc_feature    300..606
                     /label=f1 origin
                     /note="Geneious type: origin of replication"
                     /note="/Imported from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Plas
                     Mapper</a>"
                     /note="/Transferred From=f1 origin"
                     /note="/Transferred Similarity=99.67%"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     309..341
                     /label=NMG-306
                     /note="color: black; sequence: 
                     GCGGCGGGTGTGGTGGTTACGCGCAGCGTGACC"
     primer_bind     complement(420..439)
                     /label=835_LacZ-rev1
                     /note="color: black; sequence: CCCCGATTTAGAGCTTGACG"
     primer_bind     complement(789..815)
                     /label=NMG-1103
                     /note="color: black; sequence: CGGATACATATTTGAATGTATTTAGAA"
     promoter        800..828
                     /label=amp prom
                     /note="/Imported from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Plas
                     Mapper</a>"
                     /note="/Transferred From=amp prom"
                     /note="/Transferred Similarity=100.00%"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(809..831)
                     /label=NMG-1469
                     /note="color: black; sequence: GTTATTGTCTCATGAGCGGATAC"
     primer_bind     complement(859..888)
                     /label=NMG-396
                     /note="color: black; sequence: 
                     GGAAATGTTGAATACTCATACTCTTCCTTT"
     misc_feature    870..1730
                     /label=amp marker
                     /note="Geneious type: marker"
                     /note="/Imported from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Plas
                     Mapper</a>"
                     /note="/Transferred From=amp marker"
                     /note="/Transferred Similarity=99.42%"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             870..1730
                     /label=Amp-R
                     /note="/vntifkey=4"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(965..989)
                     /label=ACK-13
                     /note="color: black; sequence: TGCACCCAACTGATCTTCAGCATCT"
     primer_bind     complement(995..1022)
                     /label=563_Amp_Rev
                     /note="color: black; sequence: 
                     GCTGTTGAGATCCAGTTCGATGTAACCC"
     primer_bind     995..1022
                     /label=562_Amp_Fwd
                     /note="color: black; sequence: 
                     GGGTTACATCGAACTGGATCTCAACAGC"
     primer_bind     complement(1017..1046)
                     /label=NMG-585
                     /note="color: black; sequence: 
                     GCGAAAACTCTCAAGGATCTTACCGCTGTT"
     primer_bind     complement(1088..1107)
                     /label=QB0005 Amp-R
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     primer_bind     complement(1178..1206)
                     /label=NMG-854
                     /note="color: black; sequence: 
                     CCGTAAGATGCTTTTCTGTGACTGGTGAG"
     primer_bind     complement(1305..1324)
                     /label=ACK563
                     /note="color: black; sequence: TTGTGCAAAAAAGCGGTTAG"
     primer_bind     complement(1355..1381)
                     /label=NMG-1102
                     /note="color: black; sequence: ATGGCTTCATTCAGCTCCGGTTCCCAA"
     primer_bind     complement(1465..1489)
                     /label=ACK-12
                     /note="color: black; sequence: TCTATTAATTGTTGCCGGGAAGCTA"
     primer_bind     complement(1551..1580)
                     /label=NMG-584
                     /note="color: black; sequence: 
                     CTCACCGGCTCCAGATTTATCAGCAATAAA"
     primer_bind     complement(1710..1738)
                     /label=NMG-853
                     /note="color: black; sequence: 
                     TCTGACAGTTACCAATGCTTAATCAGTGA"
     misc_feature    1885..2504
                     /label=pBR322 origin
                     /note="Geneious type: origin of replication"
                     /note="/Imported from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Plas
                     Mapper</a>"
                     /note="/Transferred From=pBR322 origin"
                     /note="/Transferred Similarity=99.68%"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(1964..1988)
                     /label=ACK-11
                     /note="color: black; sequence: CTCTTGATCCGGCAAACAAACCACC"
     primer_bind     1969..1988
                     /label=832_BR322-fwd1
                     /note="color: black; sequence: TTTGTTTGCCGGATCAAGAG"
     primer_bind     2122..2141
                     /label=QB0767 RepO-R
                     /note="color: black; sequence: TCCTGTTACCAGTGGCTGCT"
     primer_bind     complement(2150..2169)
                     /label=833_BR322-rev1
                     /note="color: black; sequence: CGGTAAGACACGACTTATCG"
     primer_bind     2165..2191
                     /label=NMG-309
                     /note="color: black; sequence: TACCGGGTTGGACTCAAGACGATAGTT"
     primer_bind     complement(2248..2266)
                     /label=QB0766 RepO-F
                     /note="color: black; sequence: GTGTAGGTCGTTCGCTCCA"
     primer_bind     complement(2254..2281)
                     /label=NMG-851
                     /note="color: black; sequence: 
                     AGGTATCTCAGTTCGGTGTAGGTCGTTC"
     primer_bind     2409..2428
                     /label=BR322-F
                     /note="color: black; sequence: TATAGTCCTGTCGGGTTTCG"
     primer_bind     complement(2465..2489)
                     /label=ACK-10
                     /note="color: black; sequence: TTTCCATAGGCTCCGCCCCCCTGAC"
     primer_bind     2498..2519
                     /label=11_pFYF_Seq_Rev
                     /note="color: black; sequence: CAACGCGGCCTTTTTACGGTTC"
     primer_bind     2589..2608
                     /label=VR
                     /note="color: black; sequence: ATTACCGCCTTTGAGTGAGC"
     primer_bind     2643..2660
                     /label=QB0039 L4440
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     primer_bind     complement(2714..2740)
                     /label=NMG-1101
                     /note="color: black; sequence: AACCTGTCGTGCCAGCTGCATTAATGA"
     primer_bind     2774..2798
                     /label=ACK-32
                     /note="color: black; sequence: AATTAATGTGAGTTAGCTCACTCAT"
     primer_bind     complement(2774..2798)
                     /label=ACK-21
                     /note="color: black; sequence: ATGAGTGAGCTAACTCACATTAATT"
     primer_bind     2787..2806
                     /label=Lac-F (EtonSeq)
                     /note="color: black; sequence: TAGCTCACTCATTAGGCACC"
     primer_bind     2787..2806
                     /label=836_Lac-F
                     /note="color: black; sequence: TAGCTCACTCATTAGGCACC"
     promoter        2813..2842
                     /label=lac prom
                     /note="/Imported from=<a 
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Plas
                     Mapper</a>"
                     /note="/Transferred From=lac prom"
                     /note="/Transferred Similarity=100.00%"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     2855..2878
                     /label=M13-R (-46)
                     /note="color: black; sequence: GAGCGGATAACAATTTCACACAGG"
     primer_bind     2855..2878
                     /label=QB0108 Tac promoter
                     /note="color: black; sequence: GAGCGGATAACAATTTCACACAGG"
     primer_bind     2856..2878
                     /label=QB0050 M13/pUC Reverse
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     primer_bind     2875..2891
                     /label=M13-R (-26)
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     2875..2891
                     /label=Genewiz M13R
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     2875..2891
                     /label=Eton M13R Reverse
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     2875..2891
                     /label=QB0048 M13 Reverse
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     complement(2877..2903)
                     /label=654_CAG_fwd
                     /note="color: black; sequence: GGCGTAATCATGGTCATAGCTGTTTCC"
     primer_bind     complement(2877..2895)
                     /label=NMG-1452
                     /note="color: black; sequence: CATGGTCATAGCTGTTTCC"
     repeat_region   2918..3047
                     /label=5'-ITR pSub201(+)
                     /note="/modified by=jonlevy"
                     /note="/vntifkey=34"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(2929..2940)
                     /label=QB0039 L4440
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     primer_bind     2990..3001
                     /label=QB0039 L4440
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     primer_bind     3030..3053
                     /label=816_px601-fwd
                     /note="color: black; sequence: 
                     CCATCACTAGGGGTTCCTGCGGCCGCCCATAGAGCCCACCGCATCC"
     primer_bind     3035..3088
                     /label=911_px601_Xba/Kpn-U6-removal
                     /note="color: black; sequence: 
                     ACTAGGGGTTCCTGCGGCCTCTAGATCAGGGTACCCGTTACATAACTTACGGTA"
     promoter        3064..3868
                     /label=CBh promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        3072..3357
                     /label=CMV Enhancer
                     /note="/NC_006273.2=175569-175266"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     3236..3268
                     /label=NMG-839
                     /note="color: black; sequence: 
                     ACGCCCCCTATTGACGTCAATGACGGTAAATGG"
     promoter        3370..3631
                     /label=Chicken B-actin promoter
                     /note="/X00182.1=280-540, +2"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     3400..3411
                     /label=ACK64 (end)
                     /note="color: black; sequence: CCTTGCTGTCCTGCCCCACCCCACC"
     primer_bind     3406..3425
                     /label=pCAGGS-F3-Seq
                     /note="color: black; sequence: CCCACCCCCAATTTTGTATT"
     primer_bind     complement(3558..3577)
                     /label=pCAGGS-R1-Seq
                     /note="color: black; sequence: AGGAAACTTTCGGAGCGCGC"
     promoter        3608..3613
                     /label=CBA TATA Box
                     /note="/created by=User"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        3632..3771
                     /label=Chicken B-actin intron
                     /note="/X00182.1=541-677"
                     /note="/created by=User"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        3638
                     /label=putative cba +1
                     /note="/created by=User"
                     /note="color: #ffffff"
     promoter        3726..3734
                     /label=b-actin SD (MaxEnt)
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        3777..3833
                     /label=MVM Intron
                     /note="/NC_001510.1=2312-2368"
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        3779..3787
                     /label=MVM SD
                     /note="/created by=User"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     3792..3851
                     /label=830_MVM-fwd-bridge2
                     /note="color: black; sequence: 
                     AAGGGATGGTTGGTTGGTGGGGTATTAATGTTTAATTACCTGGAGCACCTGCCTGAAAT
                     C"
     primer_bind     3802..3821
                     /label=743_Cbh_f_seq
                     /note="color: black; sequence: TGGTTGGTGGGGTATTAATG"
     primer_bind     3832..3891
                     /label=831_MVM-fwd-bridge3
                     /note="color: black; sequence: 
                     TGGAGCACCTGCCTGAAATCACTTTTTTTCAGGTTGGACCGGTGCCACCATGGTGAGCA
                     A"
     promoter        3834..3868
                     /label=MVM Intron 2
                     /note="/From=methods of 
                     http://dx.doi.org/10.1038/sj.mt.6300355"
                     /note="/created by=User"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     3850..3895
                     /label=342_CBh_F
                     /note="color: black; sequence: 
                     TCACTTTTTTTCAGGTTGGACCGGTGCCACCATGGTGAGCAAGGGC"
     primer_bind     3850..3880
                     /label=798_Cas9-FLAG-NLS-fwd
                     /note="color: black; sequence: 
                     TCACTTTTTTTCAGGTTGGACCGGTGCCACC"
     promoter        complement(3861..3865)
                     /label=MVM SA (MaxEnt)
                     /note="/created by=User"
                     /note="color: #ffffff; direction: LEFT"
     CDS             3881..4597
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="color: #993366"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     3922..3942
                     /label=NMG-1457
                     /note="color: black; sequence: CATCCTGGTCGAGCTGGACGG"
     primer_bind     complement(3922..3942)
                     /label=NMG-1456
                     /note="color: black; sequence: CCGTCCAGCTCGACCAGGATG"
     primer_bind     complement(3927..3948)
                     /label=EGFP-N
                     /note="color: black; sequence: ACGTCGCCGTCCAGCTCGACCA"
     primer_bind     complement(3927..3947)
                     /label=Genewiz EGFP-N
                     /note="color: black; sequence: CGTCGCCGTCCAGCTCGACCA"
     primer_bind     3978..3999
                     /label=EGFP_380
                     /note="color: black; sequence: AGGGCGAGGGCGATGCCACCTA"
     primer_bind     complement(4012..4031)
                     /label=Eton GFP Reverse
                     /note="color: black; sequence: TGGTGCAGATGAACTTCAGG"
     primer_bind     complement(4188..4207)
                     /label=EXFP-R
                     /note="color: black; sequence: GGTCTTGTAGTTGCCGTCGT"
     primer_bind     complement(4232..4252)
                     /label=NMG-1453
                     /note="color: black; sequence: GATGCGGTTCACCAGGGTGTC"
     primer_bind     4232..4252
                     /label=NMG-1454
                     /note="color: black; sequence: GACACCCTGGTGAACCGCATC"
     primer_bind     4483..4502
                     /label=NMG-1455
                     /note="color: black; sequence: CCTGAGCACCCAGTCCGCCC"
     primer_bind     4535..4556
                     /label=EGFP-C
                     /note="color: black; sequence: ATGGTCCTGCTGGAGTTCGTGA"
     primer_bind     4570..4589
                     /label=Eton GFP (C' terminal, CFP, YFP or BFP)
                     /note="color: black; sequence: CACTCTCGGCATGGACGAGC"
     primer_bind     complement(4576..4597)
                     /label=921_mCherry-rev
                     /note="color: black; sequence: CTTGTACAGCTCGTCCATGCCG"
     primer_bind     4578..4618
                     /label=922_KASH-fwd
                     /note="color: black; sequence: 
                     GCATGGACGAGCTGTACAAGTCCGGACTCAGATCTCGAGAG"
     CDS             4610..4864
                     /label=KASH domain
                     /note="/created by=jonlevy"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(4846..4887)
                     /label=918_KASH_W3-rev
                     /note="color: black; sequence: 
                     AATCCAGAGGTTGATTATCAGATCTAGGTGGGAGGTGGCCCG"
     terminator      4869..5029
                     /label=WPRE_Gamma
                     /note="/created by=User"
                     /note="/modified by=User"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(4925..4945)
                     /label=QB0112 WPRE-R
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
     primer_bind     complement(4947..4964)
                     /label=pWPTRev
                     /note="color: black; sequence: CATTAAAGCAGCGTATCC"
     primer_bind     complement(5097..5127)
                     /label=412_CW3/bGH_rev
                     /note="color: black; sequence: 
                     GGCACAGTCGCACCACGGAATTGTCAGTGCC"
     primer_bind     5108..5140
                     /label=411_CW3/bGH_fwd
                     /note="color: black; sequence: 
                     TTCCGTGGTGCGACTGTGCCTTCTAGTTGCCAG"
     polyA_signal    5118..5328
                     /label=BGH\pA
                     /note="/vntifkey=25"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     5118..5138
                     /label=435_bGH_QPCRF1
                     /note="color: black; sequence: CGACTGTGCCTTCTAGTTGCC"
     primer_bind     5144..5162
                     /label=436_bGH_QPCRF2
                     /note="color: black; sequence: TCTGTTGTTTGCCCCTCCC"
     primer_bind     complement(5267..5291)
                     /label=ACK64 (end)
                     /note="color: black; sequence: CCTTGCTGTCCTGCCCCACCCCACC"
     primer_bind     complement(5281..5300)
                     /label=438_bGH_QPCRR2
                     /note="color: black; sequence: AATCCTCCCCCTTGCTGTCC"
     primer_bind     complement(5286..5304)
                     /label=439_bGH_QPCRR3
                     /note="color: black; sequence: TCCCAATCCTCCCCCTTGC"
     primer_bind     complement(5306..5325)
                     /label=437_bGH_QPCRR1
                     /note="color: black; sequence: CCAGCATGCCTGCTATTGTC"
     primer_bind     complement(5326..5371)
                     /label=816_px601-fwd
                     /note="color: black; sequence: 
                     CCATCACTAGGGGTTCCTGCGGCCGCCCATAGAGCCCACCGCATCC"
     primer_bind     complement(5326..5345)
                     /label=920_bGH-BamHI-rev
                     /note="color: black; sequence: CCATAGAGCCCACCGCATCC"
     primer_bind     complement(5348..5366)
                     /label=911_px601_Xba/Kpn-U6-removal
                     /note="color: black; sequence: 
                     ACTAGGGGTTCCTGCGGCCTCTAGATCAGGGTACCCGTTACATAACTTACGGTA"
ORIGIN
        1 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
       61 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagcctt aattaaccta
      121 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      181 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      241 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
      301 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      361 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      421 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
      481 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
      541 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
      601 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
      661 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
      721 tattaacgtt tataatttca ggtggcatct ttcggggaaa tgtgcgcgga acccctattt
      781 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      841 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      901 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      961 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     1021 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1081 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1141 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1201 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1261 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1321 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1381 taccaaacga cgagcgtgac accacgatgc ctgtagtaat ggtaacaacg ttgcgcaaac
     1441 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1501 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     1561 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     1621 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1681 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1741 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1801 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1861 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1921 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1981 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2041 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2101 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2161 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2221 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2281 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2341 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2401 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2461 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2521 tggccttttg ctgcggtttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2581 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2641 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     2701 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     2761 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     2821 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     2881 acagctatga ccatgattac gccagattta attaaggctg cgcgctcgct cgctcactga
     2941 ggccgcccgg gcaaagcccg ggcgtcgggc gacctttggt cgcccggcct cagtgagcga
     3001 gcgagcgcgc agagagggag tggccaactc catcactagg ggttcctgcg gcctctagat
     3061 cagggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     3121 ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc aatgggtgga
     3181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     3241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tgtgcccagt acatgacctt
     3301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga
     3361 ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt
     3421 gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcg
     3481 cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
     3541 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
     3601 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgacgc tgccttcgcc
     3661 ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg accgcgttac
     3721 tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag ctgagcaaga
     3781 ggtaagggtt taagggatgg ttggttggtg gggtattaat gtttaattac ctggagcacc
     3841 tgcctgaaat cacttttttt caggttggac cggtgccacc atggtgagca agggcgagga
     3901 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     3961 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     4021 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     4081 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     4141 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     4201 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     4261 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     4321 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     4381 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     4441 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     4501 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     4561 cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat ctcgagagga
     4621 ggaggaggag acagacagca ggatgcccca cctcgacagc cccggcagct cccagccgag
     4681 acgctccttc ctctcaaggg tgatcagggc agcgctaccg ttgcagctgc ttctgctgct
     4741 gctgctgctc ctggcctgcc tgctacctgc ctctgaagat gactacagct gcacccaggc
     4801 caacaacttt gcccgatcct tctaccccat gctgcggtac accaacgggc cacctcccac
     4861 ctagatctga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     4921 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4981 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca
     5041 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     5101 ctgacaattc cgtggtgcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     5161 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     5221 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     5281 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     5341 tatgggcggc cgcaggaacc cctagtgatg gagttgg
//
