LOCUS       Exported                9583 bp DNA     circular SYN 24-OCT-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 24, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        41..72
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             142..465
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /note="This feature has 2 segments:
                        1: 142 .. 144 / #993366
                        2: 145 .. 465 / #993366"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             481..1326
                     /codon_start=1
                     /label=CASP9
                     /note="color: #993366"
                     /translation="GFGDVGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRT
                     RTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHG
                     CQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFE
                     VASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRD
                     PKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQMPGCFNFLRKKLFFKT
                     S"
     CDS             1336..1392
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 1389"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1393..1989
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     enhancer        2053..2356
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        2357..2560
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             2599..3309
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /note="color: #ff0000"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    3316..3904
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(3787..3798)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 3786"
                     /translation="IEGR"
     LTR             3991..4224
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    4296..4417
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      4457..4592
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        complement(4624..4642)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(4652..4668)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      4810..5265
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        5291..5395
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             5396..6256
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5396 .. 5464 / #ccffcc / signal sequence
                        2: 5465 .. 6256 / #ccffcc
                     Cleavage site after base 5464"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6427..7015
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    7303..7324
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        7339..7369
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 7339 .. 7344 / #ffffff / -35
                        2: 7345 .. 7362 / #ffffff
                        3: 7363 .. 7369 / #ffffff / -10"
     protein_bind    7377..7393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     7401..7417
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        7438..7456
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        7484..7710
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             7711..7891
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    7938..8063
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    8556..8789
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    9285..9402
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        9409..9583
                     /label=TCF7 RE
                     /note="color: #ffffff; direction: RIGHT"
ORIGIN
        1 ggcgcgcccg cgtgctagcc cgggctcgag atctagactc tagagggtat ataatggaag
       61 ctcgaattcc agcttggcat tccggtactg ttggtaaaaa gcttggcatt ccggtactgt
      121 tggtaaagcc accttaatta aatgggagtg caggtggaaa ccatctcccc aggagacggg
      181 cgcaccttcc ccaagcgcgg ccagacctgc gtggtgcact acaccgggat gcttgaagat
      241 ggaaagaaag ttgattcctc ccgggacaga aacaagccct ttaagtttat gctaggcaag
      301 caggaggtga tccgaggctg ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc
      361 aaactgacta tatctccaga ttatgcctat ggtgccactg ggcacccagg catcatccca
      421 ccacatgcca ctctcgtctt cgatgtggag cttctaaaac tggaatctgg cggtggatcc
      481 ggatttggtg atgtcggtgc tcttgagagt ttgaggggaa atgcagattt ggcttacatc
      541 ctgagcatgg agccctgtgg ccactgcctc attatcaaca atgtgaactt ctgccgtgag
      601 tccgggctcc gcacccgcac tggctccaac atcgactgtg agaagttgcg gcgtcgcttc
      661 tcctcgctgc atttcatggt ggaggtgaag ggcgacctga ctgccaagaa aatggtgctg
      721 gctttgctgg agctggcgca gcaggaccac ggtgctctgg actgctgcgt ggtggtcatt
      781 ctctctcacg gctgtcaggc cagccacctg cagttcccag gggctgtcta cggcacagat
      841 ggatgccctg tgtcggtcga gaagattgtg aacatcttca atgggaccag ctgccccagc
      901 ctgggaggga agcccaagct ctttttcatc caggcctgtg gtggggagca gaaagaccat
      961 gggtttgagg tggcctccac ttcccctgaa gacgagtccc ctggcagtaa ccccgagcca
     1021 gatgccaccc cgttccagga aggtttgagg accttcgacc agctggacgc catatctagt
     1081 ttgcccacac ccagtgacat ctttgtgtcc tactctactt tcccaggttt tgtttcctgg
     1141 agggacccca agagtggctc ctggtacgtt gagaccctgg acgacatctt tgagcagtgg
     1201 gctcactctg aagacctgca gtccctcctg cttagggtcg ctaatgctgt ttcggtgaaa
     1261 gggatttata aacagatgcc tggttgcttt aatttcctcc ggaaaaaact tttctttaaa
     1321 acatcaggat ccggcgcaac aaacttctct ctgctgaaac aagccggaga tgtcgaagag
     1381 aatcctggac cgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     1441 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     1501 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     1561 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     1621 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     1681 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     1741 cccaaggagc ccgcgtggtt cctggccacc gtcggagtct cgcccgacca ccagggcaag
     1801 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     1861 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     1921 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     1981 ggtgcctgat gatcattcga aatagtaatc aattacgggg tcattagttc atagcccata
     2041 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     2101 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     2161 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     2221 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2281 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2341 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     2401 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     2461 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     2521 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
     2581 ccgctagcgc taccggtcat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     2641 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     2701 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     2761 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     2821 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     2881 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     2941 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     3001 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     3061 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     3121 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     3181 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     3241 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     3301 tacaagtaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3361 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3421 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     3481 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     3541 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     3601 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3661 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     3721 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3781 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3841 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3901 ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     3961 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     4021 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     4081 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     4141 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     4201 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     4261 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     4321 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4381 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     4441 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4501 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     4561 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattg acgtacccaa
     4621 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     4681 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     4741 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     4801 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4861 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4921 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4981 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5041 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5101 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5161 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5221 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     5281 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5341 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5401 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5461 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5521 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5581 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5641 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5701 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5761 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5821 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5881 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5941 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6001 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6061 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6121 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6181 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6241 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6301 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6361 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6421 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6481 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6541 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     6601 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6661 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6721 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6781 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6841 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6901 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6961 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7021 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7081 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7141 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     7201 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     7261 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     7321 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     7381 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     7441 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     7501 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     7561 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     7621 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     7681 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
     7741 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7801 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7861 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     7921 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     7981 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     8041 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     8101 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     8161 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     8221 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     8281 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     8341 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     8401 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     8461 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     8521 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     8581 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     8641 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     8701 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     8761 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     8821 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     8881 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     8941 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     9001 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     9061 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     9121 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     9181 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     9241 agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg
     9301 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     9361 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca cgagactagc
     9421 ctcctagccc gggctcgagc agctgaagct tgcatgcctg cagggtaccg agctcttacg
     9481 cgagatcaaa gggggtaaga tcaaaggggg taagatcaaa ggggcgcgag atcaaagggg
     9541 gtaagatcaa agggggtaag atcaaagggg gtaagatcaa agg
//
