LOCUS       GC3-MBP                 7386 bp    DNA     circular UNA 19-JUN-2022
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local...l-eym43th
VERSION     urn.local...l-eym43th
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    1..153
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="DddA_GC3 gibson extraction_bb - DddA_GC3
                     (concatenated sequence 1)"
     misc_feature    136..153
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    136..1311
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="pHJR003_pA-M.CviPI_TEV_MBP extraction_pcr1
                     (concatenated sequence 2)"
     CDS             154..174
                     /standard_name="TEV site"
     misc_feature    <154..>1293
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="M._CviPI_N-terminal_MBP_v2 - MBP for
                     pRHJ003 (concatenated sequence 2)"
     primer_bind     154..175
                     /standard_name="pHJR003_pA-M.CviPI_TEV_MBP extraction_pcr1
                     (1 F) - DddA_GC3 gibson extraction_bb"
     misc_feature    <154..>1293
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="M.CvIPI_nostartorGS (concatenated sequence
                     1)"
     misc_feature    <154..>1293
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="ProteinA_KingstonBB (concatenated sequence
                     2)"
     CDS             175..195
                     /note="/Transferred_From=TT-006_Entry_Plasmid_M., pBL82B
                     HT LaminB1"
                     /standard_name="GS Linker"
     misc_feature    175..195
                     /note="/Transferred_From=TT-006_Destination_Pla,
                     TT-006_Entry_Plasmid_M."
                     /standard_name="2x GGS Linker"
     CDS             196..1293
                     /standard_name="MBP"
     primer_bind     complement(1275..1293)
                     /standard_name="pHJR003_pA-M.CviPI_TEV_MBP extraction_pcr1
                     (1140 R) - DddA_GC3 gibson extraction_bb"
     misc_feature    1294..1296
                     /note="Geneious type: Editing History Insertion"
                     /standard_name
     misc_feature    1294..1311
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    1294..7386
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="DddA_GC3 gibson extraction_bb extraction
                     (concatenated sequence 3)"
     misc_feature    join(<1297..7278,7369..>153)
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="DddA_TC2 f2_bb (concatenated sequence 1)"
     misc_feature    1448..1819
                     /standard_name="DddI"
     misc_feature    7006..153
                     /standard_name="DddA_GC3"
     primer_bind     complement(7258..7284)
                     /standard_name="hjr061_HJR083_GC3"
     misc_feature    7261..7278
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
     misc_feature    7261..7386
                     /note="Geneious type: Concatenated sequence"
                     /standard_name="DddA_TC2 f1 (concatenated sequence 2)"
     primer_bind     7279..7302
                     /standard_name="hjr058_HJR083_GC3 "
     misc_feature    7279..7284
                     /standard_name="A1341V N1342S"
     primer_bind     complement(7345..7368)
                     /standard_name="hjr059_HJR083_GC3 "
     primer_bind     7366..7386
                     /standard_name="hjr060_HJR083_GC3 "
     misc_feature    7366..7368
                     /standard_name="E1370K"
     misc_feature    7369..7386
                     /note="Geneious type: ligation"
                     /standard_name="Ligation"
ORIGIN      
        1 gtgaacatga ttgagactct tctgcccgaa aacgccaaga tgaccgttgt tccacctgaa
       61 ggagcgatac cagtcaagcg tggcgcaact ggtgagacaa aagtgtttat tgggaatagc
      121 aattctccga aatcccccac gaaaggaggt tgtgagaacc tgtacttcca gggtggcggt
      181 tccggcggag ggtcgaaaat cgaagaaggt aaactggtaa tctggattaa cggcgataaa
      241 ggctataacg gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc
      301 accgttgagc atccggataa actggaagag aaattcccac aggttgcggc aactggcgat
      361 ggccctgaca ttatcttctg ggcacacgac cgctttggtg gctacgctca atctggcctg
      421 ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat
      481 gccgtacgtt acaacggcaa gctgattgct tacccgatcg ctgttgaagc gttatcgctg
      541 atttataaca aagatctgct gccgaacccg ccaaaaacct gggaagagat cccggcgctg
      601 gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccgtac
      661 ttcacctggc cgctgattgc tgctgacggg ggttatgcgt tcaagtatga aaacggcaag
      721 tacgacatta aagacgtggg cgtggataac gctggcgcga aagcgggtct gaccttcctg
      781 gttgacctga ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct
      841 gcctttaata aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg gtccaacatc
      901 gacaccagca aagtgaatta tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc
      961 aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa caaagagctg
     1021 gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag gtctggaagc ggttaataaa
     1081 gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg aagagttggc gaaagatcca
     1141 cgtattgccg ccaccatgga aaacgcccag aaaggtgaaa tcatgccgaa catcccgcag
     1201 atgtccgctt tctggtatgc cgtgcgtact gcggtgatca acgccgccag cggtcgtcag
     1261 actgtcgatg aagccctgaa agacgcgcag acttgagcgg ccgcataatg cttaagtcga
     1321 acagaaagta atcgtattgt acacggccgc ataatcgaaa ttaatacgac tcactatagg
     1381 ggaattgtga gcggataaca attccccatc ttagtatatt agttaagtat aagaaggaga
     1441 tatacatatg tacgcagacg atttcgacgg cgaaattgag atcgatgagg ttgatagctt
     1501 ggtcgagttc ctcagcagaa gacctgcatt tgacgcaaat aattttgttt tgacgtttga
     1561 ggaaagtggt tttcctcaat tgaatatttt tgcaaaaaac gatatcgccg ttgtttatta
     1621 tatggacatc ggcgagaatt tcgtttcgaa aggcaattct gcgtcaggtg gtacggagaa
     1681 gttttacgag aataagcttg gcggggaagt agacctgtcg aaagattgcg ttgtgagtaa
     1741 agagcaaatg attgaagcgg caaagcagtt ttttgctacg aagcaacggc ccgagcaact
     1801 gacatggagc gagttgtaaa gatctcaatt ggatatcggc cggccacgcg atcgctgacg
     1861 tcggtaccct cgagtctggt aaagaaaccg ctgctgcgaa atttgaacgc cagcacatgg
     1921 actcgtctac tagcgcagct taattaacct aggctgctgc caccgctgag caataactag
     1981 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
     2041 tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     2101 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     2161 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     2221 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     2281 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     2341 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     2401 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     2461 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttctgg
     2521 cggcacgatg gcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2581 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     2641 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     2701 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     2761 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     2821 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     2881 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     2941 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3001 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3061 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3121 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3181 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3241 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3301 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3361 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3421 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3481 tgaatactca tactcttcct ttttcaatca tgattgaagc atttatcagg gttattgtct
     3541 catgagcgga tacatatttg aatgtattta gaaaaataaa caaataggtc atgaccaaaa
     3601 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3661 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3721 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3781 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3841 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3901 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3961 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     4021 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     4081 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     4141 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     4201 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     4261 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     4321 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     4381 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     4441 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac
     4501 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     4561 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4621 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     4681 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     4741 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     4801 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     4861 tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata
     4921 ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta
     4981 ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc
     5041 actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag
     5101 cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc
     5161 agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt
     5221 ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta
     5281 aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gctagtcatg
     5341 ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat
     5401 cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc
     5461 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     5521 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg
     5581 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
     5641 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc
     5701 ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat
     5761 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
     5821 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
     5881 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
     5941 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
     6001 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
     6061 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
     6121 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg
     6181 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
     6241 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
     6301 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
     6361 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
     6421 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
     6481 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct
     6541 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
     6601 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     6661 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
     6721 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     6781 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     6841 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatcg
     6901 atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg gataacaatt
     6961 cccctctaga aataattttg tttaacttta agaaggagat ataccatggg cagcagccat
     7021 caccatcatc accacagcca ggatccgata ggactcaacg gtggggcgaa cgtctatcat
     7081 tacgcaccta acccggttgg atgggtcgat ccgtggggtt tggccggcag ttacgcgctt
     7141 ggcccctatc agatttccgc tccgcagttg ccggcatata atggacagac ggtcggaacg
     7201 ttctactacg tgaatgacgc aggtggcctt gaatccaagg tgttcatttc tggcggacct
     7261 actccatacc ccaactacgt ctctgccggg cacgttgaag gtcagtccgc cttatttatg
     7321 cgcgataacg gaatttctga aggtttggta ttccacaata accctaaagg aacgtgcggg
     7381 ttctgc
//
