LOCUS       pETDuet::dddA-dddI_MGYFPDa829        6258 bp    DNA     circular SYN 01-JAN-2025
DEFINITION  pETDuet-1.
ACCESSION   urn.local...36-iq139he
KEYWORDS    .
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     misc_feature    2..29
                     /label="lacO"
     misc_feature    47..63
                     /label="T7_transl_en_RBS"
     ORF             71..>607
                     /created_by="heejin"
                     /modified_by="heejin"
                     /label="DddA_MGYFPDa829"
     primer_bind     95..112
                     /%GC="66.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="6.0"
                     /Tm="60.1"
                     /annotation_group="10467546728370090378100-5264-48be-bcbb-
                     75cc3bce8777"
                     /created_by="primer3"
                     /Sequence="CACCACAGCCAGGATCCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pETDuet::dddA-dddI_MGYFPDa829"
                     /label="gblock_F"
     misc_feature    113..607
                     /Original_Bases="AATTCGAGCTCGGCGCGCCTGCAGGTCGACAAGCTT"
                     /label="AATTCGAGCTCGGCGCGCCTGCAGGTCGACAAGCTT"
                     /note="Geneious type: Editing History Replacement"
     promoter        673..691
                     /label="T7_promoter"
     misc_feature    691..718
                     /label="lacO"
     ORF             759..1142
                     /created_by="heejin"
                     /modified_by="heejin"
                     /label="DddI_MGYFPDa829"
     misc_feature    762..1142
                     /Original_Bases="GC"
                     /label="GC"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     1140..1161
                     /%GC="31.8"
                     /Hairpin_Tm="35.0"
                     /Self_Dimer_Tm="5.4"
                     /Tm="51.3"
                     /annotation_group="1022984002301008f25a0aa-2519-41dd-993a-
                     74a201730516"
                     /created_by="primer3"
                     /Sequence="TAAAGATCTCAATTGGATATCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pETDuet::dddA-dddI_MGYFPDa829"
                     /label="gblock_R"
     misc_feature    complement(1286..1304)
                     /label="T7_Terminal_primer"
     gene            complement(1954..2814)
                     /gene="Ampicillin"
                     /label="Ampicillin"
     promoter        complement(2857..2885)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="AmpR_promoter"
     rep_origin      2972..3591
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="pBR322_origin"
     misc_feature    3990..4012
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="pGEX_3_primer"
     misc_feature    complement(4006..4197)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="ROP"
     misc_feature    complement(4769..5860)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="lacI"
     gene            complement(5952..6203)
                     /gene="tet (887 - 636)"
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
                     /label="tet (887 - 636)"
     misc_feature    6135..6154
                     /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                     LMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECETSNVIRCRRVCRCLLSD
                     RFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTVVADWRCHLQSGPARAV
                     ANCRGD*"
                     /label="pBRrevBam_primer"
ORIGIN      
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cggacttcca
      121 cacctattac gttggcaccg aatccgtact ggttcataac aacggtggta actgcatgaa
      181 aaccgcgagc gctctggcac agggtggttc caaaggcggt tctacggcaa tcaacctgcc
      241 ggagtacgat ggtaaaacca cgcacggcgt tctggttctg gacaatggta cccaggtgca
      301 gctggtgtct ggtaatggcg atcctcgcta cactaattat cgcaacaacg gccacgttga
      361 acagaaagct gcgatctaca tgcgcgaaaa caacatttcc aacgcaactg tttaccacaa
      421 caataccaac ggcacgtgtg gctactgtaa caccatgact gccacctttc tgccggaagg
      481 tgctactctg actgtggttc ctcctaaaaa tgccgtcgca aacaactccc gcgcgatcgc
      541 atacgttaaa acgtacaccg gcacctctaa cgacccgaaa atgtctagcc gttacaaagg
      601 caactgagcg gccgcataat gcttaagtcg aacagaaagt aatcgtattg tacacggccg
      661 cataatcgaa attaatacga ctcactatag gggaattgtg agcggataac aattccccat
      721 cttagtatat tagttaagta taagaaggag atatacatat gctggtcgaa cactttatgg
      781 gtcagaaaga atgcgattcc ctggaagagc tgcgtgaagt cctgagcgaa cgtactgaaa
      841 aaggtgttaa cgaatttatc atcagcactc acgaacagtt cccttacatg atcatgagcg
      901 ttaaagaaaa atacgcttgt ctgtcttact tccgtgaaga ggacgatccg ggttacagct
      961 ctgtgaacgc taacccggtt ctggacgctg acggtattag cattttctat accaacaccg
     1021 attctgaaga aattgaggtg gctaactaca gcatcgtcaa aatcgaagac gctgtgagcg
     1081 ccgttgagga attctttgaa actctgcagc tgccgaaatg tatcgagtgg gaagaactgt
     1141 aaagatctca attggatatc ggccggccac gcgatcgctg acgtcggtac cctcgagtct
     1201 ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca tggactcgtc tactagcgca
     1261 gcttaattaa cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     1321 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat
     1381 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     1441 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     1501 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     1561 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     1621 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     1681 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     1741 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     1801 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc tggcggcacg atggcatgag
     1861 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1921 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1981 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2041 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     2101 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     2161 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     2221 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     2281 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     2341 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2401 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2461 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2521 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2581 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2641 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2701 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2761 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2821 cctttttcaa tcatgattga agcatttatc agggttattg tctcatgagc ggatacatat
     2881 ttgaatgtat ttagaaaaat aaacaaatag gtcatgacca aaatccctta acgtgagttt
     2941 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3001 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3061 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     3121 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     3181 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3241 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3301 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3361 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3421 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3481 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3541 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3601 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3661 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3721 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     3781 cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct
     3841 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     3901 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     3961 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     4021 gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga
     4081 ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa
     4141 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     4201 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag
     4261 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg
     4321 taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca
     4381 gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca
     4441 gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg
     4501 gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct
     4561 tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc
     4621 tagccgggtc ctcaacgaca ggagcacgat catgctagtc atgccccgcg cccaccggaa
     4681 ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt
     4741 gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4801 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4861 ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct
     4921 ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct
     4981 gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca
     5041 ctaccgagat gtccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca
     5101 gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt
     5161 gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct
     5221 gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag
     5281 aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca
     5341 cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag
     5401 agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct
     5461 ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca
     5521 ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac
     5581 ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca
     5641 gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc
     5701 ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag
     5761 aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact
     5821 ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg
     5881 ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga
     5941 cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg
     6001 agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc
     6061 acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc
     6121 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
     6181 gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tcgatctcga tcccgcgaaa
     6241 ttaatacgac tcactata
//
