LOCUS       Exported                7717 bp ds-DNA     circular SYN 29-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Doudna Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(40..94)
                     /label=oBA1714_p15_cmR_part4_gib_R
     primer_bind     complement(69..91)
                     /label=oBA1740_pt4_commonGib_R
     primer_bind     complement(73..94)
                     /label=oBA1651_pBFC0924_Gib_R
     misc_feature    88..120
                     /label=gbBA079
     misc_feature    88..91
                     /label=4>5
     misc_feature    92..116
                     /label=ConS scar
     primer_bind     92..116
                     /label=oBA1097_conS_Scar_check_F
     protein_bind    92..95
                     /label=BsmBI
     primer_bind     complement(99..135)
                     /label=HS_690
     primer_bind     106..142
                     /label=HS_689
     misc_feature    117..120
                     /label=Pt2/2a BsaI Junction Scar
     terminator      121..487
                     /label=TrrnB terminator (BBa_K1893035)
                     /label=TrrnB
     misc_feature    121..124
                     /label=DN
     misc_feature    122
                     /label=a>t removes HindIII
     misc_feature    join(208,221)
                     /label=covariant muts to remove BsaI
     misc_feature    join(390,407)
                     /label=covariant muts to remove BsaI
     terminator      421..466
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(460..487)
                     /label=oBFC2037
     misc_feature    484..487
                     /label=BsmBI_OH
     primer_bind     487..515
                     /label=oBFC1697
     primer_bind     487..515
                     /label=PTet_F
     misc_feature    488..491
                     /label=1>2
     misc_feature    492..1231
                     /label=PCR from pba369
     primer_bind     492..507
                     /label=oBA1632_EJC_pTET_p2_F
     primer_bind     492..506
                     /label=oBFC2038
     primer_bind     492..506
                     /label=oBFC0336
     CDS             complement(499..1125)
                     /label=tetR
     primer_bind     1015..1041
                     /label=KEW.A01
     primer_bind     1066..1088
                     /label=oBFC0419
     primer_bind     complement(1085..1122)
                     /label=oBFC0604
     promoter        1126..1198
                     /label=tet promoter region
     primer_bind     1126..1169
                     /label=oBFC1701
     protein_bind    1147..1165
                     /label=Q1
     primer_bind     complement(1162..1196)
                     /label=oBFC0337
     primer_bind     complement(1162..1196)
                     /label=oBFC0338
     primer_bind     complement(1163..1198)
                     /label=OTT345
     primer_bind     complement(1169..1203)
                     /label=HS_672
     primer_bind     complement(1174..1198)
                     /label=oBFC1704
     primer_bind     1176..1194
                     /label=oBFC1899
     protein_bind    1177..1193
                     /label=Q2
     primer_bind     complement(1178..1195)
                     /label=oBFC1708
     primer_bind     complement(1179..1213)
                     /label=oBFC2046
     primer_bind     complement(1185..1231)
                     /label=oBA1681_oBA1120_GG_pBA368_Gib_F
     primer_bind     complement(1191..1231)
                     /label=oBA1833_taCas13_specialGGBB_R
     primer_bind     complement(1192..1235)
                     /label=oBFC1698
     primer_bind     complement(1192..1235)
                     /label=PTet_R
     primer_bind     complement(1192..1235)
                     /label=oBA2200_pBA1169_BB_Gib_R
     primer_bind     complement(1192..1213)
                     /label=oBA1595_Cas13_BB_CommonGib_R
     primer_bind     complement(1192..1213)
                     /label=oBA1596_Cas13_BB_CommonGib_R
     primer_bind     complement(1194..1236)
                     /label=oBA2304_pTet_PCR_Gib_prep_R
     primer_bind     complement(1194..1236)
                     /label=oBA2487_Ham31DELhamA_Mut_Gib_R
     primer_bind     1204..1258
                     /label=oBA2805_Pfl_HamAB_Fus3_1_F
     RBS             1214..1231
                     /label=RBS
     misc_feature    1232..1233
                     /label=2>3
     misc_feature    1234..2960
                     /label=MA_swap_chunk
     CDS             1234..2358
                     /codon_start=1
                     /label=PfHamM
                     /translation="MKISMHDAAYGDSILISGASTKILVDGGTAKSFADWSPSLEGVDT
                     LDALIVTHIDNDHIGGAIKLLTSKHATKVKEVYFNGINQILDVEDCDIGDRSDDLELSA
                     IIANTTSRDEDQLKGYSEGTSLSLILQQQNIPLNKATEGRQIHRTSLNKNIIIGEFEIE
                     LIGPSRKSLLKIQQKWLELLESKEIKRRLISPRYSVAFESFISSIDTSVKHDEEISSLS
                     ENIEHLANQPFTNDTSPPNKSSISFLLKTENKSILMLGDSDAETIEAWLNSKSADSITV
                     DAVKLPHHGSRNNFSANLVRRIRTANYLISTNGDKFSHPNIETLARIIKFSPDREAKIL
                     LNHEIPHLTNSFLSNANSYEGNISIHQGIKEFEL"
     misc_feature    1234..2349
                     /label=M_swap_chunk
     primer_bind     1234..1257
                     /label=OTT328
     primer_bind     1234..1257
                     /label=OTT336
     primer_bind     1234..1257
                     /label=OTT362
     misc_feature    1235..2833
                     /standard_name="gbBA273"
                     /label=gbBA273
                     /note="Geneious type: note"
     misc_feature    1235..2310
                     /standard_name="HamM"
                     /label=HamM
                     /note="Geneious type: note"
     gene            1235..2310
                     /pseudo
                     /locus_tag="B723_06625"
                     /standard_name="gene"
                     /note="hypothetical protein; disrupted; Derived by
                     automated computational analysis using gene prediction 
                     method: Protein Homology."
     primer_bind     complement(2325..2349)
                     /label=OTT363
     misc_feature    2355..3809
                     /locus_tag="B723_06620"
                     /standard_name="hypothetical protein highlight"
                     /product="hypothetical protein"
                     /label=hypothetical protein highlight
                     /note="Geneious type: highlight"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
     CDS             2355..3809
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="B723_06620"
                     /standard_name="hypothetical protein CDS"
                     /product="hypothetical protein"
                     /label=hypothetical protein CDS
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AKV06087.1"
                     /translation="MSNALTDEYKLDNVFILEAYDDLGEPISTGSCFSVGASIFFTSWH
                     VVKNAYTLRIFQSRDDYFRQEFHAAKCVFYDKETDFAILSSDSLTIKSKIEIGIYKAEL
                     NDEVKAIGYAAEKSRLHFPITSKISNVFDFSTTYKYDFEFGKPAELDKANGLSGGPVFF
                     KGYAIGLLALQTVGSALQAVSFHTIFERPSALEAIESQGLVITGGDEISYDRPDHPESP
                     FVVSIDCAASDPNIKGLDIGFDLGEWRSQNLIDQTLNWIEDYALTPKQKKALGQHNFKR
                     IQSAKRNFQIGNPAAMADLFLHIAIRQNYKTIPIISNVITVDGESVFSCSHIVIYRGKV
                     ELWLGISAMEVSMEQTVKEAIHRVDSIIRSGGIQDRLVVILSELDPTWPYIKKLEKLSN
                     SNLKLTDKIDKIIIPMFLSSNADLINNYDPSNFSNSFQKDIDECRERVAGAYNARIVPL
                     INLRIFLFPVDNIHELHTKFVKEFEF"
     gene            2355..3809
                     /locus_tag="B723_06620"
                     /standard_name="gene"
     misc_feature    2355..2960
                     /label=protease domain
     misc_feature    2361..2960
                     /label=Aprot_swap_chunk
     primer_bind     2361..2385
                     /label=OTT364
     misc_feature    2803..4602
                     /standard_name="gbBA274"
                     /label=gbBA274
                     /note="Geneious type: note"
     primer_bind     2803..2828
                     /label=oBA2807_Pfl_HamAB_Fus3_2_F
     primer_bind     complement(2814..2833)
                     /label=oBA2806_Pfl_HamAB_Fus3_1_R
     primer_bind     complement(2937..2960)
                     /label=OTT333
     primer_bind     complement(2937..2960)
                     /label=OTT337
     primer_bind     complement(2937..2960)
                     /label=OTT365
     misc_feature    3339..3341
                     /label=inactive S --> D
     misc_feature    3390..3392
                     
     misc_feature    3396..3398
                     /label=putative inactive A-->K
     misc_feature    3806..5977
                     /standard_name="HamB"
                     /label=HamB
                     /note="Geneious type: note"
     CDS             3806..5977
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="B723_06615"
                     /standard_name="hypothetical protein CDS"
                     /product="hypothetical protein"
                     /label=hypothetical protein CDS
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AKV06086.1"
                     /translation="MNTEIYNNLRKQIDNSAFTGWQRLFVELINLYNLHDDNQFLIQDL
                     TLRCLERRGEFKQLHGLLNILISELGLFPYLDPDLMTSKDSIRMNIFKSPIAGDITFHL
                     KQAEIYYRIVNGDNIVLSAPTSFGKSLVIDALISSEEYDNIVVVVPTIALMDELRKKFS
                     KYKENYKIITQSSQEPATKNLYIYTQERVLECKNLERVDFFIIDEFYKLAPLNTSDYRS
                     DRLNLAFHKLFKMCSRFYMLGPNINGLAEGIEDKLNCNFVKYDNYKTVVANEYRYIIRE
                     DGNDKEKDIDRDAHLVNILQKIGQNEQTLIYCKTPKRAASLVKKIMKLRENQEIRKGEF
                     SDWLRETYHDDWSMADAVERGIACHHGRLPRVVSGYVVEAFNEKLINILVCTSTLIEGV
                     NTNARSVIIYDDCIRGTTQLDSFTFNNISGRSGRMFEHFVGDIHVIGRKPSAELPWIDV
                     PIVTQDDSASRPMLLSIIDELSTEQRASLSRYTTQQYVPITLLNKHLGVPPERIIKFAE
                     ALMEKCGAWNSIMAWTGKFPTSEQLRHLCNIMFDHFAISRMGGGCVKSKKQLFYRVLNI
                     VNRKEDKDLIQSEYDFWSPRNAEYEIDDAVQSVFDFKRNLIGYNLPKIILAINDVQRLI
                     FSRFNYRCGDYAAFAHSLEAMFLPPSIVALEEFGIPTRVAQKIYQISNVGEDATVDLIL
                     EQVRSHPGLYISKFDDFEKTLLNRAIQYL"
     gene            3806..5977
                     /locus_tag="B723_06615"
                     /standard_name="gene"
     primer_bind     complement(4561..4602)
                     /label=oBA2808_Pfl_HamAB_Fus3_2_R
     misc_feature    4573..5977
                     /label=gbBA275
     primer_bind     4573..4613
                     /label=oBA2809_Pfl_HamAB_Fus3_3_F
     primer_bind     complement(5948..6007)
                     /label=oBA2810_Pfl_HamAB_Fus3_3_R
     primer_bind     5977..6017
                     /label=oBA1713_p15_cmR_part4_gib_F
     primer_bind     5977..5996
                     /label=oBA1739_pt4_commonGib_F
     primer_bind     5977..5996
                     /label=oBA2199_pBA1169_BB_Gib_F
     primer_bind     5977..5993
                     /label=oBA1650_pBFC0924_Gib_F
     misc_feature    join(5978..7717,1..66)
                     /label=PCR from pBFC0335 or similar
     misc_feature    5978..5996
                     /label=Pt4_common_region_6>4
     misc_feature    5978..5996
                     /label=introduced
     primer_bind     5978..5996
                     /label=oBA2305_oBA1904_pt4ONLY_common_F
     misc_feature    5978..5981
                     /label=6>4 OH
     misc_feature    complement(5982..5985)
                     /label=SMRT Gate OH Right
     misc_feature    complement(5990..5996)
                     /label=AarI
     primer_bind     5996..6016
                     /label=oBFC1058_fixed
     primer_bind     5996..6016
                     /label=oBFC1060_fixed
     primer_bind     5996..6016
                     /label=oBFC1062_fixed
     primer_bind     5996..6016
                     /label=oBFC1447
     misc_feature    complement(5997..6105)
                     /label=CamR Terminator
     misc_feature    complement(5997..6105)
                     /label=CamR Terminator
     primer_bind     5997..6022
                     /label=oBA2031_WYL_p15a_CmR_Gib_F
     primer_bind     5997..6016
                     /label=OTT344
     CDS             complement(6106..6765)
                     /label=CamR
     misc_feature    6532..6535
                     /label=BbsI OH
     promoter        complement(6766..6870)
                     /label=CamR Promoter
     promoter        complement(6766..6870)
                     /label=CamR Promoter
     rep_origin      complement(7138..7683)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     rep_origin      complement(7138..7683)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
ORIGIN
        1 agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata
       61 agttgtcacc tgcccgaacc agagacttat cctgaactgg ccgataattg cagacgaacg
      121 atgcttgggc ccgaacaaaa actcatctca gaagaggatc tgaatagcgc cgtcgaccat
      181 catcatcatc atcattgagt ttaaacgctc tccagcttgg gtgttttggc ggatgagaga
      241 agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt
      301 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
      361 gccgtagcgc cgatggtagt gtggggtctg cccatgcgag agtaggcaac tgccaggcat
      421 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      481 gtgaacttca gcgacgtctt aagacccact ttcacattta agttgttttt ctaatccgca
      541 tatgatcaat tcaaggccga ataagaaggc tggctctgca ccttggtgat caaataattc
      601 gatagcttgt cgtaataatg gcggcatact atcagtagta ggtgtttccc tttcttcttt
      661 agcgacttga tgctcttgat cttccaatac gcaacctaaa gtaaaatgcc ccacagcgct
      721 gagtgcatat aatgcattct ctagtgaaaa accttgttgg cataaaaagg ctaattgatt
      781 ttcgagagtt tcatactgtt tttctgtagg ccgtgtacct aaatgtactt ttgctccatc
      841 gcgatgactt agtaaagcac atctaaaact tttagcgtta ttacgtaaaa aatcttgcca
      901 gctttcccct tctaaagggc aaaagtgagt atggtgccta tctaacatct caatggctaa
      961 ggcgtcgagc aaagcccgct tattttttac atgccaatac aatgtaggct gctctacacc
     1021 tagcttctgg gcgagtttac gggttgttaa accttcgatt ccgacctcat taagcagctc
     1081 taatgcgctg ttaatcactt tacttttatc taatctagac atcattaatt cctaattttt
     1141 gttgacactc tatcgttgat agagttattt taccactccc tatcagtgat agagaaaaga
     1201 attcaaaaga tcttttaaga aggagatata ctgatgaaaa tttccatgca cgatgccgca
     1261 tacggtgatt caattctcat cagcggcgcc tcgaccaaaa ttttagtgga tggtggaact
     1321 gcgaagtcct tcgctgactg gtcgccttcg ttagagggtg tcgatacgct agatgccctg
     1381 atagttactc atattgataa cgaccatatt ggcggagcaa tcaagctgct cacatcaaag
     1441 cacgcaacta aagtaaaaga agtttatttc aatggcatta accaaatcct agatgtggag
     1501 gattgtgata ttggtgatcg tagtgacgat ctagaattat ccgcaatcat agcaaacacc
     1561 acgtctagag acgaagacca gttgaaaggg tattctgaag gcacatcgtt atccttaata
     1621 ttacagcagc aaaatatccc cttaaataaa gcaaccgaag gcaggcaaat ccacagaacg
     1681 agccttaata aaaacataat aataggagaa tttgaaattg aactgatagg cccaagcaga
     1741 aagagcttgt taaaaatcca acaaaaatgg cttgaactat tagaaagtaa agaaatcaaa
     1801 agacgcctta tcagcccaag atatagcgtt gcattcgaaa gctttatcag ctcgattgat
     1861 acctcagtaa agcatgatga ggaaatttca agtctaagtg aaaacataga gcatttggca
     1921 aatcaaccat ttactaatga cacatcaccc ccaaacaaaa gcagcatctc tttccttcta
     1981 aaaactgaaa acaaaagcat tttaatgctc ggagattccg acgcagagac tatagaggca
     2041 tggttgaact cgaagtctgc agatagcatc acagttgatg cggtaaagct accccatcac
     2101 ggaagccgaa ataactttag tgctaactta gtacgcagaa tacgcactgc gaactattta
     2161 atttctacta atggcgataa attttcacac cctaacattg agaccttagc tagaatcatt
     2221 aagttttctc ctgaccgcga ggcaaaaata ctactaaacc acgaaatccc ccacctcaca
     2281 aactccttcc tgagcaacgc caacagttat gaaggaaata tcagcatcca ccagggcata
     2341 aaggaatttg agctttgagt aatgcactga ctgacgagta caaactcgat aacgtcttta
     2401 tcctagaagc ctatgacgac ctaggtgaac ccatttcaac aggcagttgc ttttcggtgg
     2461 gtgcgtctat tttttttaca tcttggcacg tcgtcaaaaa tgcctacacg cttcggattt
     2521 tccaaagtag agatgattat tttcgtcaag agttccatgc cgctaaatgt gttttttacg
     2581 ataaagaaac tgacttcgca atactgtcat cagacagcct tacgatcaaa tcaaaaattg
     2641 aaataggaat atacaaagct gagctgaatg atgaggtaaa agctataggg tacgcggccg
     2701 aaaaaagcag attgcatttc cctataacta gtaaaatatc aaacgtcttt gatttctcta
     2761 caacatataa atatgacttt gagtttggaa agccggctga gctagacaaa gcaaatggct
     2821 tatccggggg gccagtattc tttaaaggct acgctattgg cttgcttgca ctgcaaactg
     2881 tgggatcggc attgcaagca gtatctttcc atacaatatt tgaacggcca tccgctcttg
     2941 aagccattga aagccaaggg ttggttataa ccggtggtga cgaaatatcc tatgataggc
     3001 cagaccatcc tgaaagccca tttgttgtct caatagactg tgccgcttcg gatccaaaca
     3061 taaagggact agatatagga tttgatcttg gcgaatggcg cagtcagaac ctgattgatc
     3121 aaacgctcaa ctggatcgaa gactacgcgc tgaccccgaa gcaaaaaaaa gctttggggc
     3181 aacacaactt caaaagaatt caaagtgcaa aaagaaactt ccagattggc aacccagcag
     3241 caatggccga tttattctta cacatcgcga ttcgccaaaa ttataaaacc atccctatta
     3301 tttcaaatgt aataacggta gatggcgagt ctgtgttttc ctgctcgcat atcgttattt
     3361 atagaggcaa agttgagcta tggcttggaa tctctgctat ggaggtttcc atggagcaaa
     3421 cagtaaagga ggctattcac agagttgact ccatcatacg ttccggagga atccaagacc
     3481 ggttggttgt tatattatca gaactagacc ctacttggcc gtatataaaa aagctggaaa
     3541 aattatcaaa ctccaacctc aaattaactg acaaaattga taagataatt atacctatgt
     3601 ttttatcaag caatgcagac ttgataaata attatgaccc aagcaatttt tcaaactcat
     3661 ttcaaaaaga tatagatgag tgcagggaaa gggttgctgg cgcctacaat gctcgaatcg
     3721 ttcccttgat caacctccgc atctttctat ttccggtcga caacatacac gagttacata
     3781 cgaaatttgt taaggagttc gaattttgaa tacggaaatt tataacaacc ttcgtaagca
     3841 aatcgacaac tctgctttca ccggatggca aaggttattc gttgaactta taaatctata
     3901 caacttacat gatgacaatc aattcttgat tcaggacctg acgctgagat gcctagagag
     3961 gcgtggggaa ttcaaacagc ttcatggtct gttgaacata ttgatttccg agctaggttt
     4021 gttcccgtac ttagatccag atctgatgac gtcgaaagac agcataagga tgaatatttt
     4081 taagtcgcct atagctggcg atataacatt ccacctaaaa caggctgaga tttattacag
     4141 gattgtaaat ggagataaca tcgtactcag tgcacctaca agcttcggaa aaagcttagt
     4201 tatagatgct ctcatcagct ctgaagaata cgacaacatt gtggtagtag tcccaaccat
     4261 agctttgatg gatgagttac gaaagaaatt tagcaaatat aaagaaaact acaagataat
     4321 cactcagtcc tctcaagagc cagcgacaaa aaatctatat atctatactc aagaaagggt
     4381 cttggaatgc aagaaccttg aacgagtaga cttctttatt atcgatgagt tttacaaatt
     4441 agcacctctc aatacatctg actatcgaag cgacagacta aatctggcat ttcacaaact
     4501 ttttaaaatg tgcagtcgct tttacatgct cggccccaac attaatggcc tggccgaagg
     4561 catagaagac aaactaaatt gcaattttgt aaaatatgac aattacaaaa ctgtagtagc
     4621 taacgaatat cgctacatta tccgtgaaga tgggaatgat aaagaaaaag acattgatcg
     4681 cgacgcgcac ttagtcaata ttttgcaaaa aatcggtcaa aacgaacaga ctttaattta
     4741 ctgcaagact ccaaaacgag cagcctcgct agtaaaaaag atcatgaaac tcagagagaa
     4801 ccaagagata agaaaaggag agttttctga ctggctgaga gagacatatc acgatgactg
     4861 gtcgatggca gacgcagtgg aacgcgggat tgcctgtcac catggcagac ttcctcgtgt
     4921 cgtctccggg tatgtggtgg aagcgttcaa tgaaaagctc ataaatattt tggtttgcac
     4981 ctcaactttg attgagggtg ttaacacaaa tgctagaagc gtcataattt acgatgactg
     5041 tattcgaggg accacccaac tggattcgtt cacttttaac aatatctctg gacgtagtgg
     5101 gcgtatgttt gaacactttg ttggagatat acatgttatt ggacgaaaac catcagcaga
     5161 gcttccttgg atcgacgtcc caatagtgac tcaagatgac tccgcctcta gacccatgct
     5221 gctaagcata atcgacgagc taagcaccga acaaagggct agtttatcga gatatacaac
     5281 tcaacaatat gtacctatca ctttacttaa taagcatctc ggtgttcctc cggaaaggat
     5341 aataaaattc gcagaagcct taatggagaa atgtggcgcc tggaactcaa ttatggcatg
     5401 gacgggaaaa ttcccaacta gcgagcaact aagacaccta tgcaacatca tgttcgatca
     5461 cttcgcaata tcacgaatgg gcggaggatg cgtcaaatca aaaaagcagc tgttttatag
     5521 agtgctcaac attgtaaata gaaaagaaga caaagatcta attcaaagcg aatatgattt
     5581 ttggtcccca cgaaacgcgg aatatgagat tgatgacgca gtgcaaagcg ttttcgattt
     5641 caaaagaaac ttaattggct acaacctccc taagattatt ttggcgataa acgacgtaca
     5701 gcgcttaata ttttctcgat tcaattaccg ttgtggcgac tacgcagctt ttgcccactc
     5761 tctagaagca atgtttttac caccttcaat tgtggctctt gaagagtttg gaataccaac
     5821 tcgtgtagca caaaaaatat accaaattag caacgtcggc gaggacgcca ccgtagattt
     5881 aatcctagag caggtaagat cgcacccggg tctctacata tcgaaatttg atgattttga
     5941 aaaaactctc ctgaaccgag caattcaata tctttaaagg aggactttgg caggtgacca
     6001 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     6061 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     6121 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     6181 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     6241 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     6301 actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt
     6361 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     6421 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     6481 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     6541 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     6601 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     6661 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     6721 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     6781 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     6841 aaagttggaa cctcttacgt gcccgatcaa gcgctagcgg agtgtatact ggcttactat
     6901 gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac
     6961 cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc
     7021 tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc
     7081 tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt
     7141 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
     7201 aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc
     7261 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca
     7321 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg
     7381 aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7441 cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta
     7501 gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct
     7561 cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg
     7621 ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct
     7681 caagaagatc atcttattaa tcagataaaa tatttct
//
