LOCUS       Exported                6874 bp ds-DNA     circular SYN 29-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Doudna Lab
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(40..94)
                     /label=oBA1714_p15_cmR_part4_gib_R
     primer_bind     complement(69..91)
                     /label=oBA1740_pt4_commonGib_R
     primer_bind     complement(73..94)
                     /label=oBA1651_pBFC0924_Gib_R
     misc_feature    88..120
                     /label=gbBA079
     misc_feature    88..91
                     /label=4>5
     misc_feature    92..116
                     /label=ConS scar
     primer_bind     92..116
                     /label=oBA1097_conS_Scar_check_F
     protein_bind    92..95
                     /label=BsmBI
     primer_bind     complement(99..135)
                     /label=HS_690
     primer_bind     106..142
                     /label=HS_689
     misc_feature    117..120
                     /label=Pt2/2a BsaI Junction Scar
     terminator      121..487
                     /label=TrrnB terminator (BBa_K1893035)
                     /label=TrrnB
     misc_feature    121..124
                     /label=DN
     misc_feature    122
                     /label=a>t removes HindIII
     misc_feature    join(208,221)
                     /label=covariant muts to remove BsaI
     misc_feature    join(390,407)
                     /label=covariant muts to remove BsaI
     terminator      421..466
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(460..487)
                     /label=oBFC2037
     misc_feature    484..487
                     /label=BsmBI_OH
     primer_bind     487..515
                     /label=oBFC1697
     primer_bind     487..515
                     /label=PTet_F
     misc_feature    488..491
                     /label=1>2
     misc_feature    492..1231
                     /label=PCR from pba369
     primer_bind     492..507
                     /label=oBA1632_EJC_pTET_p2_F
     primer_bind     492..506
                     /label=oBFC2038
     primer_bind     492..506
                     /label=oBFC0336
     CDS             complement(499..1125)
                     /label=tetR
     primer_bind     1015..1041
                     /label=KEW.A01
     primer_bind     1066..1088
                     /label=oBFC0419
     primer_bind     complement(1085..1122)
                     /label=oBFC0604
     promoter        1126..1198
                     /label=tet promoter region
     primer_bind     1126..1169
                     /label=oBFC1701
     protein_bind    1147..1165
                     /label=Q1
     primer_bind     complement(1162..1196)
                     /label=oBFC0337
     primer_bind     complement(1162..1196)
                     /label=oBFC0338
     primer_bind     complement(1163..1198)
                     /label=OTT345
     primer_bind     complement(1169..1203)
                     /label=HS_672
     primer_bind     complement(1174..1198)
                     /label=oBFC1704
     primer_bind     1176..1194
                     /label=oBFC1899
     protein_bind    1177..1193
                     /label=Q2
     primer_bind     complement(1178..1195)
                     /label=oBFC1708
     primer_bind     complement(1179..1213)
                     /label=oBFC2046
     primer_bind     complement(1185..1231)
                     /label=oBA1681_oBA1120_GG_pBA368_Gib_F
     primer_bind     complement(1191..1231)
                     /label=oBA1833_taCas13_specialGGBB_R
     primer_bind     complement(1192..1235)
                     /label=oBFC1698
     primer_bind     complement(1192..1235)
                     /label=PTet_R
     primer_bind     complement(1192..1235)
                     /label=oBA2200_pBA1169_BB_Gib_R
     primer_bind     complement(1192..1213)
                     /label=oBA1595_Cas13_BB_CommonGib_R
     primer_bind     complement(1192..1213)
                     /label=oBA1596_Cas13_BB_CommonGib_R
     primer_bind     complement(1194..1236)
                     /label=oBA2304_pTet_PCR_Gib_prep_R
     primer_bind     complement(1194..1236)
                     /label=oBA2487_Ham31DELhamA_Mut_Gib_R
     primer_bind     1204..1258
                     /label=oBA2805_Pfl_HamAB_Fus3_1_F
     RBS             1214..1231
                     /label=RBS
     misc_feature    1232..1233
                     /label=2>3
     misc_feature    1234..2960
                     /label=MA_swap_chunk
     CDS             1234..2358
                     /codon_start=1
                     /label=PfHamM
                     /translation="MKISMHDAAYGDSILISGASTKILVDGGTAKSFADWSPSLEGVDT
                     LDALIVTHIDNDHIGGAIKLLTSKHATKVKEVYFNGINQILDVEDCDIGDRSDDLELSA
                     IIANTTSRDEDQLIGYSEGTSLSLILQQQNIPLNKATEGRQIHRTSLNKNIIIGEFEIE
                     LIGPSRKSLLKIQQKWLELLESKEIKRRLISPRYSVAFESFISSIDTSVKHDEEISSLS
                     ENIEHLANQPFTNDTSPPNKSSISFLLKTENKSILMLGDSDAETIEAWLNSKSADSITV
                     DAVKLPHHGSRNNFSANLVRRIRTANYLISTNGDKFSHPNIETLARIIKFSPDREAKIL
                     LNHEIPHLTNSFLSNANSYEGNISIHQGIKEFEL"
     misc_feature    1234..2349
                     /label=M_swap_chunk
     primer_bind     1234..1257
                     /label=OTT328
     primer_bind     1234..1257
                     /label=OTT336
     primer_bind     1234..1257
                     /label=OTT362
     misc_feature    1235..2833
                     /standard_name="gbBA273"
                     /label=gbBA273
                     /note="Geneious type: note"
     misc_feature    1235..2310
                     /standard_name="HamM"
                     /label=HamM
                     /note="Geneious type: note"
     gene            1235..2310
                     /pseudo
                     /locus_tag="B723_06625"
                     /standard_name="gene"
                     /note="hypothetical protein; disrupted; Derived by
                     automated computational analysis using gene prediction 
                     method: Protein Homology."
     primer_bind     complement(2325..2349)
                     /label=OTT363
     misc_feature    2355..2966
                     /locus_tag="B723_06620"
                     /standard_name="hypothetical protein highlight"
                     /product="hypothetical protein"
                     /label=hypothetical protein highlight
                     /note="Geneious type: highlight"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
     CDS             2355..2966
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="B723_06620"
                     /standard_name="hypothetical protein CDS"
                     /product="hypothetical protein"
                     /label=hypothetical protein CDS
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AKV06087.1"
                     /translation="MSNALTDEYKLDNVFILEAYDDLGEPISTGSCFSVGASIFFTSWH
                     VVKNAYTLRIFQSRDDYFRQEFHAAKCVFYDKETDFAILSSDSLTIKSKIEIGIYKAEL
                     NDEVKAIGYAAEKSRLHFPITSKISNVFDFSTTYKYDFEFGKPAELDKANGLSGGPVFF
                     KGYAIGLLALQTVGSALQAVSFHTIFERPSALEAIESQGF"
     gene            2355..2966
                     /locus_tag="B723_06620"
                     /standard_name="gene"
     misc_feature    2355..2960
                     /label=protease domain
     misc_feature    2361..2960
                     /label=Aprot_swap_chunk
     primer_bind     2361..2385
                     /label=OTT364
     misc_feature    2803..3759
                     /standard_name="gbBA274"
                     /label=gbBA274
                     /note="Geneious type: note"
     primer_bind     2803..2828
                     /label=oBA2807_Pfl_HamAB_Fus3_2_F
     primer_bind     complement(2814..2833)
                     /label=oBA2806_Pfl_HamAB_Fus3_1_R
     primer_bind     complement(2937..2960)
                     /label=OTT333
     primer_bind     complement(2937..2960)
                     /label=OTT337
     primer_bind     complement(2937..2960)
                     /label=OTT365
     misc_feature    2963..5134
                     /standard_name="HamB"
                     /label=HamB
                     /note="Geneious type: note"
     CDS             2963..5134
                     /codon_start=1
                     /transl_table=11
                     /locus_tag="B723_06615"
                     /standard_name="hypothetical protein CDS"
                     /product="hypothetical protein"
                     /label=hypothetical protein CDS
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /inference="EXISTENCE:same species:RefSeq"
                     /protein_id="AKV06086.1"
                     /translation="MNTEIYNNLRKQIDNSAFTGWQRLFVELINLYNLHDDNQFLIQDL
                     TLRCLERRGEFKQLHGLLNILISELGLFPYLDPDLMTSKDSIRMNIFKSPIAGDITFHL
                     KQAEIYYRIVNGDNIVLSAPTSFGKSLVIDALISSEEYDNIVVVVPTIALMDELRKKFS
                     KYKENYKIITQSSQEPATKNLYIYTQERVLECKNLERVDFFIIDEFYKLAPLNTSDYRS
                     DRLNLAFHKLFKMCSRFYMLGPNINGLAEGIEDKLNCNFVKYDNYKTVVANEYRYIIRE
                     DGNDKEKDIDRDAHLVNILQKIGQNEQTLIYCKTPKRAASLVKKIMKLRENQEIRKGEF
                     SDWLRETYHDDWSMADAVERGIACHHGRLPRVVSGYVVEAFNEKLINILVCTSTLIEGV
                     NTNARSVIIYDDCIRGTTQLDSFTFNNISGRSGRMFEHFVGDIHVIGRKPSAELPWIDV
                     PIVTQDDSASRPMLLSIIDELSTEQRASLSRYTTQQYVPITLLNKHLGVPPERIIKFAE
                     ALMEKCGAWNSIMAWTGKFPTSEQLRHLCNIMFDHFAISRMGGGCVKSKKQLFYRVLNI
                     VNRKEDKDLIQSEYDFWSPRNAEYEIDDAVQSVFDFKRNLIGYNLPKIILAINDVQRLI
                     FSRFNYRCGDYAAFAHSLEAMFLPPSIVALEEFGIPTRVAQKIYQISNVGEDATVDLIL
                     EQVRSHPGLYISKFDDFEKTLLNRAIQYL"
     gene            2963..5134
                     /locus_tag="B723_06615"
                     /standard_name="gene"
     primer_bind     complement(3718..3759)
                     /label=oBA2808_Pfl_HamAB_Fus3_2_R
     misc_feature    3730..5134
                     /label=gbBA275
     primer_bind     3730..3770
                     /label=oBA2809_Pfl_HamAB_Fus3_3_F
     primer_bind     complement(5105..5164)
                     /label=oBA2810_Pfl_HamAB_Fus3_3_R
     primer_bind     5134..5174
                     /label=oBA1713_p15_cmR_part4_gib_F
     primer_bind     5134..5153
                     /label=oBA1739_pt4_commonGib_F
     primer_bind     5134..5153
                     /label=oBA2199_pBA1169_BB_Gib_F
     primer_bind     5134..5150
                     /label=oBA1650_pBFC0924_Gib_F
     misc_feature    join(5135..6874,1..66)
                     /label=PCR from pBFC0335 or similar
     misc_feature    5135..5153
                     /label=Pt4_common_region_6>4
     misc_feature    5135..5153
                     /label=introduced
     primer_bind     5135..5153
                     /label=oBA2305_oBA1904_pt4ONLY_common_F
     misc_feature    5135..5138
                     /label=6>4 OH
     misc_feature    complement(5139..5142)
                     /label=SMRT Gate OH Right
     misc_feature    complement(5147..5153)
                     /label=AarI
     primer_bind     5153..5173
                     /label=oBFC1058_fixed
     primer_bind     5153..5173
                     /label=oBFC1060_fixed
     primer_bind     5153..5173
                     /label=oBFC1062_fixed
     primer_bind     5153..5173
                     /label=oBFC1447
     misc_feature    complement(5154..5262)
                     /label=CamR Terminator
     misc_feature    complement(5154..5262)
                     /label=CamR Terminator
     primer_bind     5154..5179
                     /label=oBA2031_WYL_p15a_CmR_Gib_F
     primer_bind     5154..5173
                     /label=OTT344
     CDS             complement(5263..5922)
                     /label=CamR
     misc_feature    5689..5692
                     /label=BbsI OH
     promoter        complement(5923..6027)
                     /label=CamR Promoter
     promoter        complement(5923..6027)
                     /label=CamR Promoter
     rep_origin      complement(6295..6840)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     rep_origin      complement(6295..6840)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
ORIGIN
        1 agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc atacgatata
       61 agttgtcacc tgcccgaacc agagacttat cctgaactgg ccgataattg cagacgaacg
      121 atgcttgggc ccgaacaaaa actcatctca gaagaggatc tgaatagcgc cgtcgaccat
      181 catcatcatc atcattgagt ttaaacgctc tccagcttgg gtgttttggc ggatgagaga
      241 agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt
      301 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
      361 gccgtagcgc cgatggtagt gtggggtctg cccatgcgag agtaggcaac tgccaggcat
      421 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      481 gtgaacttca gcgacgtctt aagacccact ttcacattta agttgttttt ctaatccgca
      541 tatgatcaat tcaaggccga ataagaaggc tggctctgca ccttggtgat caaataattc
      601 gatagcttgt cgtaataatg gcggcatact atcagtagta ggtgtttccc tttcttcttt
      661 agcgacttga tgctcttgat cttccaatac gcaacctaaa gtaaaatgcc ccacagcgct
      721 gagtgcatat aatgcattct ctagtgaaaa accttgttgg cataaaaagg ctaattgatt
      781 ttcgagagtt tcatactgtt tttctgtagg ccgtgtacct aaatgtactt ttgctccatc
      841 gcgatgactt agtaaagcac atctaaaact tttagcgtta ttacgtaaaa aatcttgcca
      901 gctttcccct tctaaagggc aaaagtgagt atggtgccta tctaacatct caatggctaa
      961 ggcgtcgagc aaagcccgct tattttttac atgccaatac aatgtaggct gctctacacc
     1021 tagcttctgg gcgagtttac gggttgttaa accttcgatt ccgacctcat taagcagctc
     1081 taatgcgctg ttaatcactt tacttttatc taatctagac atcattaatt cctaattttt
     1141 gttgacactc tatcgttgat agagttattt taccactccc tatcagtgat agagaaaaga
     1201 attcaaaaga tcttttaaga aggagatata ctgatgaaaa tttccatgca cgatgccgca
     1261 tacggtgatt caattctcat cagcggcgcc tcgaccaaaa ttttagtgga tggtggaact
     1321 gcgaagtcct tcgctgactg gtcgccttcg ttagagggtg tcgatacgct agatgccctg
     1381 atagttactc atattgataa cgaccatatt ggcggagcaa tcaagctgct cacatcaaag
     1441 cacgcaacta aagtaaaaga agtttatttc aatggcatta accaaatcct agatgtggag
     1501 gattgtgata ttggtgatcg tagtgacgat ctagaattat ccgcaatcat agcaaacacc
     1561 acgtctagag acgaagacca gttgataggg tattctgaag gcacatcgtt atccttaata
     1621 ttacagcagc aaaatatccc cttaaataaa gcaaccgaag gcaggcaaat ccacagaacg
     1681 agccttaata aaaacataat aataggagaa tttgaaattg aactgatagg cccaagcaga
     1741 aagagcttgt taaaaatcca acaaaaatgg cttgaactat tagaaagtaa agaaatcaaa
     1801 agacgcctta tcagcccaag atatagcgtt gcattcgaaa gctttatcag ctcgattgat
     1861 acctcagtaa agcatgatga ggaaatttca agtctaagtg aaaacataga gcatttggca
     1921 aatcaaccat ttactaatga cacatcaccc ccaaacaaaa gcagcatctc tttccttcta
     1981 aaaactgaaa acaaaagcat tttaatgctc ggagattccg acgcagagac tatagaggca
     2041 tggttgaact cgaagtctgc agatagcatc acagttgatg cggtaaagct accccatcac
     2101 ggaagccgaa ataactttag tgctaactta gtacgcagaa tacgcactgc gaactattta
     2161 atttctacta atggcgataa attttcacac cctaacattg agaccttagc tagaatcatt
     2221 aagttttctc ctgaccgcga ggcaaaaata ctactaaacc acgaaatccc ccacctcaca
     2281 aactccttcc tgagcaacgc caacagttat gaaggaaata tcagcatcca ccagggcata
     2341 aaggaatttg agctttgagt aatgcactga ctgacgagta caaactcgat aacgtcttta
     2401 tcctagaagc ctatgacgac ctaggtgaac ccatttcaac aggcagttgc ttttcggtgg
     2461 gtgcgtctat tttttttaca tcttggcacg tcgtcaaaaa tgcctacacg cttcggattt
     2521 tccaaagtag agatgattat tttcgtcaag agttccatgc cgctaaatgt gttttttacg
     2581 ataaagaaac tgacttcgca atactgtcat cagacagcct tacgatcaaa tcaaaaattg
     2641 aaataggaat atacaaagct gagctgaatg atgaggtaaa agctataggg tacgcggccg
     2701 aaaaaagcag attgcatttc cctataacta gtaaaatatc aaacgtcttt gatttctcta
     2761 caacatataa atatgacttt gagtttggaa agccggctga gctagacaaa gcaaatggct
     2821 tatccggggg gccagtattc tttaaaggct acgctattgg cttgcttgca ctgcaaactg
     2881 tgggatcggc attgcaagca gtatctttcc atacaatatt tgaacggcca tccgctcttg
     2941 aagccattga aagccaaggg ttttgaatac ggaaatttat aacaaccttc gtaagcaaat
     3001 cgacaactct gctttcaccg gatggcaaag gttattcgtt gaacttataa atctatacaa
     3061 cttacatgat gacaatcaat tcttgattca ggacctgacg ctgagatgcc tagagaggcg
     3121 tggggaattc aaacagcttc atggtctgtt gaacatattg atttccgagc taggtttgtt
     3181 cccgtactta gatccagatc tgatgacgtc gaaagacagc ataaggatga atatttttaa
     3241 gtcgcctata gctggcgata taacattcca cctaaaacag gctgagattt attacaggat
     3301 tgtaaatgga gataacatcg tactcagtgc acctacaagc ttcggaaaaa gcttagttat
     3361 agatgctctc atcagctctg aagaatacga caacattgtg gtagtagtcc caaccatagc
     3421 tttgatggat gagttacgaa agaaatttag caaatataaa gaaaactaca agataatcac
     3481 tcagtcctct caagagccag cgacaaaaaa tctatatatc tatactcaag aaagggtctt
     3541 ggaatgcaag aaccttgaac gagtagactt ctttattatc gatgagtttt acaaattagc
     3601 acctctcaat acatctgact atcgaagcga cagactaaat ctggcatttc acaaactttt
     3661 taaaatgtgc agtcgctttt acatgctcgg ccccaacatt aatggcctgg ccgaaggcat
     3721 agaagacaaa ctaaattgca attttgtaaa atatgacaat tacaaaactg tagtagctaa
     3781 cgaatatcgc tacattatcc gtgaagatgg gaatgataaa gaaaaagaca ttgatcgcga
     3841 cgcgcactta gtcaatattt tgcaaaaaat cggtcaaaac gaacagactt taatttactg
     3901 caagactcca aaacgagcag cctcgctagt aaaaaagatc atgaaactca gagagaacca
     3961 agagataaga aaaggagagt tttctgactg gctgagagag acatatcacg atgactggtc
     4021 gatggcagac gcagtggaac gcgggattgc ctgtcaccat ggcagacttc ctcgtgtcgt
     4081 ctccgggtat gtggtggaag cgttcaatga aaagctcata aatattttgg tttgcacctc
     4141 aactttgatt gagggtgtta acacaaatgc tagaagcgtc ataatttacg atgactgtat
     4201 tcgagggacc acccaactgg attcgttcac ttttaacaat atctctggac gtagtgggcg
     4261 tatgtttgaa cactttgttg gagatataca tgttattgga cgaaaaccat cagcagagct
     4321 tccttggatc gacgtcccaa tagtgactca agatgactcc gcctctagac ccatgctgct
     4381 aagcataatc gacgagctaa gcaccgaaca aagggctagt ttatcgagat atacaactca
     4441 acaatatgta cctatcactt tacttaataa gcatctcggt gttcctccgg aaaggataat
     4501 aaaattcgca gaagccttaa tggagaaatg tggcgcctgg aactcaatta tggcatggac
     4561 gggaaaattc ccaactagcg agcaactaag acacctatgc aacatcatgt tcgatcactt
     4621 cgcaatatca cgaatgggcg gaggatgcgt caaatcaaaa aagcagctgt tttatagagt
     4681 gctcaacatt gtaaatagaa aagaagacaa agatctaatt caaagcgaat atgatttttg
     4741 gtccccacga aacgcggaat atgagattga tgacgcagtg caaagcgttt tcgatttcaa
     4801 aagaaactta attggctaca acctccctaa gattattttg gcgataaacg acgtacagcg
     4861 cttaatattt tctcgattca attaccgttg tggcgactac gcagcttttg cccactctct
     4921 agaagcaatg tttttaccac cttcaattgt ggctcttgaa gagtttggaa taccaactcg
     4981 tgtagcacaa aaaatatacc aaattagcaa cgtcggcgag gacgccaccg tagatttaat
     5041 cctagagcag gtaagatcgc acccgggtct ctacatatcg aaatttgatg attttgaaaa
     5101 aactctcctg aaccgagcaa ttcaatatct ttaaaggagg actttggcag gtgaccaata
     5161 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
     5221 actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca
     5281 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     5341 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     5401 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     5461 ggtgaaactc acccagggat tggctgaaac gaaaaacata ttctcaataa accctttagg
     5521 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     5581 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     5641 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     5701 acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     5761 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     5821 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     5881 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     5941 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
     6001 gttggaacct cttacgtgcc cgatcaagcg ctagcggagt gtatactggc ttactatgtt
     6061 ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg
     6121 tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac
     6181 gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg
     6241 aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca
     6301 taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa
     6361 cccgacagga ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc
     6421 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
     6481 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
     6541 cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6601 aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga ggagttagtc
     6661 ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga ctgcgctcct
     6721 ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga aaaaccgccc
     6781 tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa acgatctcaa
     6841 gaagatcatc ttattaatca gataaaatat ttct
//
