LOCUS       Exported                6350 bp ds-DNA     circular SYN 29-OCT-2025
DEFINITION  Generated by Golden Gate/Type IIS Cloning 
            (pBA742_GFP_dropout_p15a_Chlor_CIRCLE, NZ_JASOKN010000009 - Chy3_Kae
            - Chy3_Kae - gbBA366__KaeChy3_p1, NZ_JASOKN010000009 - Chy3_Kae - 
            Chy3_Kae - gbBA367__KaeChy3_p2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    7..633
                     /label=tetR
     misc_feature    634..706
                     /label=tet promoter region
     misc_feature    655..673
                     /label=Q1 operator (tetR)
     misc_feature    655..673
                     /label=Q2 operator (TetR )
     misc_feature    722..741
                     /label=RBS(JBEI biobricks)
     misc_feature    738..741
                     /label=Editing History Insertion
     misc_feature    738..741
                     /label=Ligation
     misc_feature    738..741
                     /label=BsaI UP
     primer_bind     1041..1067
                     /label=OTT398
     CDS             1042..2211
                     /locus_tag="QP353_RS13850"
                     /old_locus_tag="QP353_13845"
                     /product="caspase family protein"
                     /label=caspase family protein CDS
                     /db_xref="GI:823326486"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /protein_id="WP_047080305.1"
     misc_feature    1171..1173
                     /label=L > L silent
     misc_feature    1173
                     /label=C
     primer_bind     complement(1570..1589)
                     /label=OTT385
     primer_bind     1590..1613
                     /label=OTT384
     misc_feature    1600..1602
                     /label=catalytic C
     primer_bind     complement(2186..2211)
                     /label=OTT399
     CDS             2208..3248
                     /codon_start=1
                     /label=ChyB
                     /translation="MNEVTPKNINLSIRVKSKFNFGGRDTKIDIRDSELILVEKLTTSR
                     EFSLLPGLYEVSTFLGDGKRASQIVEVFESQLTEISLEPDIDYQPAPPKDDSQFKKIEE
                     LNQDNRFGLTILDEDTDFTIYPAGSYWIVQHKDAMQKMPIITLKKNGSYIQASLPISQG
                     HRYSVTCHVKQVLNSKYGHVEINISQSRAMAFGLECMLKSGQIMNAAEVASNALEALIE
                     KYTDPTGATLAALILYKTCRLDESSKRHLINLVTHHDWLPDAKIILALYLARTGNLDES
                     MQLACEASKKRILLTESFSLLLDLLRYWPETEHDEKRSEALARLAQIAPFVIWKSMYLT
                     TSMTGD"
     primer_bind     2208..2237
                     /label=OTT400
     misc_feature    2484..2487
                     /label=Ligation
     primer_bind     complement(3226..3248)
                     /label=OTT401
     primer_bind     3249..3275
                     /label=OTT402
     CDS             3265..4332
                     /codon_start=1
                     /label=ChyC
                     /translation="MLPAREGDAILVRWGEEHSPYQMLVDMGVEETGKEFNDKISGLPD
                     TQRTFEAVVVTHIDADHIGGFLSCFVDCDEPCAIVKDFWFNGYMHLDGRRSSSSLEGLG
                     AVQAERLTTWLSTQPWNDCYDGGSVCRSEGEPLKVIELAGGMKVTVLGPTMQRLEKLKP
                     EWDKEINNALQKKQKKLSVPNLESMGASLPLEIPDAQSLKFLAKQRPLRDTKAANGSSI
                     VLLLEYDGKKVLLAGDAFAEDLVDAILVLSSEFPLKLEAFKVPAHCSRGNISKQLIEAV
                     DCTNWLISTDGSRHKHPDDEGIASILLHRTTDFVNLVFNVTSDYNSKWSNTEWKQCFRY
                     SANYGSKEDGYLLNL"
     primer_bind     complement(3541..3563)
                     /label=OTT393
     primer_bind     3564..3587
                     /label=OTT392
     misc_feature    3574..3576
                     /label=cut site G
     primer_bind     complement(3784..3818)
                     /label=OTT395
     primer_bind     3819..3846
                     /label=OTT394
     misc_feature    3829..3831
                     /label=cut site G
     primer_bind     complement(4030..4050)
                     /label=OTT391
     primer_bind     4051..4072
                     /label=OTT390
     misc_feature    4062
                     /label=catalytic H
     primer_bind     complement(4301..4333)
                     /label=OTT403
     misc_feature    4483..4486
                     /label=Editing History Insertion
     misc_feature    4483..4486
                     /label=Ligation
     misc_feature    4483..4486
                     /label=BsaI DN
     misc_feature    4507..4635
                     /label=dbI terminator
     misc_feature    4642..5353
                     /label=p15A
     misc_feature    5444..5549
                     /label=To (terminator ?)
     misc_feature    5565..6224
                     /label=cmR2
ORIGIN
        1 gacgtcttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
       61 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      121 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      181 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      241 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      301 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      361 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      421 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      481 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      541 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      601 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
      661 tcgttgatag agttatttta ccactcccta tcagtgatag agaaaagaat tcaaaagatc
      721 ttttaagaag gagatataca ttagatagat cgcactcgaa tctgacagtc tggtttgggc
      781 caggagcaga ccttcgatgt atctggaaac taggggacat tcagctgagc atagagattt
      841 aaaaatacac ttgctaagct caatgcattg aaataaagat agttgaggta aatcttcaga
      901 caatttaccg catgtaccaa aaatagccct accgaagata aacttgagga atatcagtta
      961 gcccggataa ctgatatcaa gtaaaatgcc aaaactcaag gcttaactga gccggatatc
     1021 aatcaaggag tggctcgtcg aatgactgca gttatagatg atagctcaaa ctcacccgga
     1081 acccatgcgc ttatcattgg tgtaagcgcg tatgaccatc tgatgggctc tagtgaaggg
     1141 gaatgcagtg agcaaggctc agattctggt ctgggacagc taagtgcggc cgcaagatct
     1201 gcttcccagt ttgcgacgtg gttaatgaaa gattatcgac gcaaggactt tccgcttcgg
     1261 agtttgcggg tacttttatc gccagcgact gaagacgaag tgggtgaaaa cctcaagggt
     1321 tatcttaaag aagactcaat cccaacgagg gaaaatgtat caaccgcatt tagaaaattt
     1381 aaacaggcag ccgcaagaaa tccggataat atcgcaatcg tttatgtggc aggacatgga
     1441 gttcaattta ctaatactgg agcagtactc ctattaaagg attttgccca tcctgatcat
     1501 gaggaagaac gatataaggg agctgtagat attagagcca tgcatgaaag catgacgtat
     1561 acaaacgggg cgcggcgtca gttttggttt gtggatatat gcaggcaaag agctccggat
     1621 gctaaagagt tcgaggaatt agctggtgta ttaaaattca acaaagttat caatacaggt
     1681 agttctggag cgaccatatg ctccccgcta tttctggcgg catcatcggg cgagcaagcg
     1741 tttgccaaac ctggaaagct aactcttttc tgtgaggcct tgatgtcagc ccttgttgaa
     1801 ggtcatgctg cttcctccga tggggagcgc agttcaccat ggctagtatc aactagtgga
     1861 ttacagatgt atcttagaga cactgtagaa ttgttagctg cctgtaatga tgaaaaacaa
     1921 ttaatcgaat gctcagggat ttttagcaat gaagtctttc atgaatgcga aaaagctccg
     1981 aatgtagatt taacaataca gatcaaagac ggcttgcctg gggcccacta cagtgcaaaa
     2041 ttgtctcatc tcggcagagt tctttatgat aatcatacaa catggccgct tcgcgacagc
     2101 atacctgcag ggatctataa gatagaagtt acaactctaa ccactaatga gaaagactca
     2161 gataatctca aactttcgcc tccaagtagg ataaaggaac ttccactatg aatgaggtca
     2221 cacctaaaaa cataaacctt agtattagag tgaagtctaa attcaatttc ggcggacgag
     2281 acacgaaaat cgacatccga gatagtgaac tcattcttgt tgaaaagctc actacaagca
     2341 gagagttcag tctgctcccc ggactgtatg aagttagcac atttctgggc gatggaaaga
     2401 gagcatcgca gattgttgaa gtattcgaat cacaattaac tgagataagt ttagagccag
     2461 acattgatta tcagcctgcg cctccaaaag atgacagtca gtttaagaaa atcgaggagt
     2521 tgaatcagga taatcgattt ggtttgacta ttcttgatga agatactgac tttactattt
     2581 atccggctgg aagctattgg atcgtgcaac acaaagacgc aatgcaaaag atgcctataa
     2641 taacactcaa aaaaaatggg tcgtatattc aagctagcct ccctattagc caaggccacc
     2701 gttattccgt tacttgtcat gtgaaacagg tcttaaattc gaagtatggc cacgtagaaa
     2761 taaacatctc acagtctaga gctatggctt ttggcttgga gtgtatgctc aaaagtgggc
     2821 aaattatgaa tgcagccgag gtggcttcta atgcactaga agccttgata gaaaaataca
     2881 cggaccctac aggagcaaca cttgcagcac tgattcttta caagacatgc cgacttgatg
     2941 agtcgagcaa aagacattta atcaatttgg taacccatca tgattggtta cctgatgcaa
     3001 aaataatatt agctttatac ttagcacgca ctggcaacct ggacgaatca atgcaacttg
     3061 cgtgtgaagc tagcaaaaaa cgtattcttt taactgagag cttttcatta ttattggatc
     3121 ttcttcgtta ttggccagaa acagaacacg atgagaaaag aagcgaagct ctcgcacgtt
     3181 tagcgcaaat cgcgccattc gtaatttgga agtctatgta tcttaccacc agtatgaccg
     3241 gagattaaat tgttctcaat acaaatgcta cctgcacgtg aaggcgatgc aattttggtg
     3301 cgctggggag aggaacactc tccttatcaa atgcttgtcg atatgggggt cgaggaaaca
     3361 gggaaagagt ttaatgataa aattagcggc ctgcccgata cgcagcggac ctttgaagca
     3421 gttgtagtta cacatatcga cgcagaccat attggaggat ttttgagttg ttttgttgac
     3481 tgcgatgagc cgtgcgccat agtgaaagat ttttggttta atggttacat gcatctggat
     3541 ggtcgcagaa gcagttcttc tttagaaggg ctaggtgcag ttcaagccga aaggctcact
     3601 acatggttga gcacacagcc ctggaatgat tgttatgacg gaggctcagt ctgtcgaagc
     3661 gagggagaac cgcttaaagt cattgaactc gcaggaggca tgaaggtaac tgtccttggg
     3721 cctacgatgc aaagacttga aaagcttaaa cctgaatggg ataaagaaat aaataacgct
     3781 ttacaaaaaa aacaaaaaaa actctcagta cctaacctgg aatccatggg agcaagttta
     3841 ccattggaga ttccagacgc tcagtccctc aaatttttag ctaagcaaag gcccttgcga
     3901 gacactaaag ctgcaaatgg gagcagtatt gtcttactac tagaatatga tggtaaaaaa
     3961 gttctactcg ccggcgatgc gttcgctgaa gacttggttg atgctatttt agttttatca
     4021 agtgaatttc ccctaaagct cgaagctttc aaagtccccg cccactgtag tcgtggaaat
     4081 atctcaaagc aactaattga agctgttgac tgcacaaatt ggcttatatc gacagatggc
     4141 agcagacata agcaccccga cgatgaaggg atagcgtcta tcctgcttca caggacaacc
     4201 gattttgtaa atttggtttt caatgtaact agcgattaca attcaaaatg gtcgaacacc
     4261 gagtggaaac aatgcttcag atattcagca aactatggct ctaaagaaga tggttattta
     4321 ctgaatcttt gattttataa atttatctta tttaaggtct gcgagtgctt gtagaattaa
     4381 cttccgcaac tcgctcttag cagaccacac agcccttcaa gcggtccgct actgccagaa
     4441 gcggaatttg ttaactttgt tatatgctct ttaaaaaatt atggatccaa actcgagtaa
     4501 ggatctccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     4561 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     4621 ctttctgcgt ttatacctag ggatatattc cgcttcctcg ctcactgact cgctacgctc
     4681 ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga
     4741 tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg
     4801 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg
     4861 acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt
     4921 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
     4981 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     5041 cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag
     5101 acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga
     5161 agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa
     5221 gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca
     5281 aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag
     5341 atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc tcttcaaatg
     5401 tagcacctga agtcagcccc atacgatata agttgttact agtgcttgga ttctcaccaa
     5461 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     5521 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
     5581 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
     5641 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
     5701 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     5761 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
     5821 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     5881 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     5941 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     6001 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     6061 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     6121 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     6181 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     6241 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     6301 aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccagatatc
//
