LOCUS       Exported                6937 bp ds-DNA     circular SYN 24-JUN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(37..248)
                     /label=EF-1-alpha core promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     terminator      331..370
                     /label=bacterial terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=90.9"
                     /note="/other=terminator"
     CDS             complement(371..1087)
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1106..1134)
                     /label=J23119 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=96.6"
                     /note="/match_length=82.9"
                     /note="/other=promoter"
     CDS             complement(1648..1767)
                     /label=B (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.5"
                     /note="/other=CDS"
     CDS             1769..1924
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.7"
                     /note="/other=CDS"
     rep_origin      1929..2357
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        2410..2514
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             2515..3375
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     rep_origin      3546..4134
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             4317..4409
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
     protein_bind    4422..4443
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        4458..4488
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    4496..4512
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(4550..5017)
                     /label=B (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=29.3"
                     /note="/other=CDS"
     ncRNA           complement(5712..5804)
                     /label=G-CSF SLDE
                     /note="pLannotate"
                     /note="/database=Rfam"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=96.9"
                     /note="/other=ncRNA"
     polyA_signal    complement(5824..5945)
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     misc_feature    complement(5954..6060)
                     /label=WPRE (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=96.3"
                     /note="/match_length=26.5"
                     /note="/other=misc_feature"
     CDS             complement(6067..6666)
                     /label=PuroR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.5"
                     /note="/match_length=100.2"
                     /note="/other=CDS"
     LTR             complement(join(6693..6937,1..24))
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
ORIGIN
        1 gcgtgaagga gagatgcgag cccctcgaag cttcagctgt gttctggcgg caaacccgtt
       61 gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc caccctcggg
      121 aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc accttctcta
      181 ggcacccgtt caattgccga cccctccccc caacttctcg gggactgtgg gcgatgtgcg
      241 ctctgcccac tgacgggcac cggagcgatc gcagatcctt cgcggccgcg ggcccgaatt
      301 cgaatttaaa tcggatccca gctgagacga gaaatcatcc ttagcgaaag ctaaggattt
      361 tttttatctg cttgtactac tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
      421 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc
      481 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
      541 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
      601 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
      661 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
      721 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
      781 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
      841 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
      901 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
      961 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     1021 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     1081 tcaccatcta gtatttctcc tctttgctag cactatacct aggactgagc tagccgtaaa
     1141 cgtctctcgt ttactagtgg ccgtggaggc cttttccccg tatcccccca ggtgtctgca
     1201 ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc
     1261 ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg
     1321 agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg
     1381 gctttggggg ggggctgtcc ctcataaaag ttttgttact ttatagaaga aattttgagt
     1441 ttttgttttt tttaataaat aaataaacat aaataaattg tttgttgaat ttattattag
     1501 tatgtaagtg taaatataat aaaacttaat atctattcaa attaataaat aaacctcgat
     1561 atacagaccg ataaaacaca tgcgtcaatt ttacacatga ttatctttaa cgtacgtcac
     1621 aatatgatta tctttctagg gttaatctag ctgcgtgttc tgcagcgtgt cgagcatctt
     1681 catctgctcc atcacgctgt aaaacacatt tgcaccgcga gtctgcccgt cctccacggg
     1741 ttcaaaaacg tgaatgaacg aggcgcgctc actggccgtc gttttacaac gtcgtgactg
     1801 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     1861 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     1921 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     1981 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     2041 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     2101 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     2161 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     2221 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     2281 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     2341 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     2401 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     2461 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     2521 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     2581 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2641 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2701 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     2761 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2821 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2881 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2941 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     3001 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     3061 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3121 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3181 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3241 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3301 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3361 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3421 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3481 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3541 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3601 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3661 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3721 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3781 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3841 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3901 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3961 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4021 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4081 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4141 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4201 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     4261 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     4321 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     4381 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     4441 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     4501 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcccgc
     4561 cgggtaactc acggggtatc catgtccatt tctgcggcat ccagccagga tacccgtcct
     4621 cgctgacgta atatcccagc gccgcaccgc tgtcattaat ctgcacaccg gcacggcagt
     4681 tccggctgtc gccggtattg ttcgggttgc tgatgcgctt cgggctgacc atccggaact
     4741 gtgtccggaa aagccgcgac gaactggtat cccaggtggc ctgaacgaac agttcaccgt
     4801 taaaggcgtg catggccaca ccttcccgaa tcatcatggt aaacgtgcgt tttcgctcaa
     4861 cgtcaatgca gcagcagtca tcctcggcaa actctttcca tgccgcttca acctcgcggg
     4921 aaaaggcacg ggcttcttcc tccccgatgc ccagatagcg ccagcttggg cgatgactga
     4981 gccggaaaaa agacccgacg atatgatcct gatgcagcta gattaaccct agaaagatag
     5041 tctgcgtaaa attgacgcat gcattcttga aatattgctc tctctttcta aatagcgcga
     5101 atccgtcgct gtgcatttag gacatctcag tcgccgcttg gagctcccgt gaggcgtgct
     5161 tgtcaatgcg gtaagtgtca ctgattttga actataacga ccgcgtgagt caaaatgacg
     5221 catgattatc ttttacgtga cttttaagat ttaactcata cgataattat attgttattt
     5281 catgttctac ttacgtgata acttattata tatatatttt cttgttatag atatcaggga
     5341 cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca
     5401 gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc
     5461 gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc
     5521 tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagcc ataccaatgg
     5581 gccctaaaaa aggaatccag tcaattccgg ggctaaacct ggctgccact gtttctttag
     5641 ggacttcgtt cctgtgagga caggaaaccc tgcaacctgg ttgaccagag caagggatat
     5701 aagaacccag tttgggaaca ctgctgttta aatattaaac agggatgtct tgtcccccgg
     5761 aagggagacc agatgagctt ctgatagact aattgacatc atttgagtca attccagaca
     5821 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     5881 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     5941 aagtttagtt aacgcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg
     6001 agcaacatag ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt
     6061 gtcgactcag gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc
     6121 gacgtcggcg gtgacggtga agccgagccg ctcgtagaag gggaggttgc ggggcgcgga
     6181 ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac
     6241 ggcgctgccc agacccttgc cctggtggtc gggcgacacg ccgacggtgg ccaggaacca
     6301 cgcgggctcc ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag
     6361 ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc
     6421 gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga cccacacctt
     6481 gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc agctcggtga cccgctcgat
     6541 gtggcggtcc ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag
     6601 ggtgcgtacg gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc
     6661 ggtcatctag cgtaggcgcc ggtcacagct tggatctgta acggcgcaga acagaaaacg
     6721 aaacaaagac gtagagttga gcaagcaggg tcaggcaaag cgtggagagc cggctgagtc
     6781 taggtaggct ccaagggagc gccggacaaa ggcccggtct cgacctgagc tttaaactta
     6841 cctagacggc ggacgcagtt caggaggcac cacaggcggg aggcggcaga acgcgactca
     6901 accggcgtgg atggcggcct caggtagggc ggcgggc
//
