LOCUS       Exported                8001 bp ds-DNA     circular SYN 24-JUN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Oct 30, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..8001
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             303..770
                     /label=B (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=29.3"
                     /note="/other=CDS"
     protein_bind    complement(808..824)
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        complement(832..862)
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(877..898)
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(911..1003)
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
     rep_origin      complement(1186..1774)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(1945..2805)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(2806..2910)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     rep_origin      complement(2963..3391)
                     /direction=LEFT
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(3396..3551)
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.7"
                     /note="/other=CDS"
     CDS             3553..3672
                     /label=B (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.5"
                     /note="/other=CDS"
     promoter        4186..4214
                     /label=J23119 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=96.6"
                     /note="/match_length=82.9"
                     /note="/other=promoter"
     CDS             4233..4949
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     terminator      complement(4950..4989)
                     /label=bacterial terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=90.9"
                     /note="/other=terminator"
     promoter        5087..5298
                     /label=EF-1-alpha core promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     intron          5299..6237
                     /label=EF-1-alpha intron A
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=intron"
     CDS             6256..7277
                     /label=HygR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=95.8"
                     /note="/match_length=99.6"
                     /note="/other=CDS"
     misc_feature    7294..7400
                     /label=WPRE (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=96.3"
                     /note="/match_length=26.5"
                     /note="/other=misc_feature"
     polyA_signal    7409..7530
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     ncRNA           7548..7640
                     /label=G-CSF SLDE
                     /note="pLannotate"
                     /note="/database=Rfam"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=96.9"
                     /note="/other=ncRNA"
ORIGIN
        1 aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt
       61 atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt
      121 cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc
      181 aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag
      241 agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaatct
      301 agctgcatca ggatcatatc gtcgggtctt ttttccggct cagtcatcgc ccaagctggc
      361 gctatctggg catcggggag gaagaagccc gtgccttttc ccgcgaggtt gaagcggcat
      421 ggaaagagtt tgccgaggat gactgctgct gcattgacgt tgagcgaaaa cgcacgttta
      481 ccatgatgat tcgggaaggt gtggccatgc acgcctttaa cggtgaactg ttcgttcagg
      541 ccacctggga taccagttcg tcgcggcttt tccggacaca gttccggatg gtcagcccga
      601 agcgcatcag caacccgaac aataccggcg acagccggaa ctgccgtgcc ggtgtgcaga
      661 ttaatgacag cggtgcggcg ctgggatatt acgtcagcga ggacgggtat cctggctgga
      721 tgccgcagaa atggacatgg ataccccgtg agttacccgg cgggcgcgct tggcgtaatc
      781 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      841 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      901 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      961 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     1021 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     1081 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1141 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1201 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1261 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1321 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1381 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1441 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1501 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1561 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1621 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1681 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1741 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1801 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1861 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1921 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     1981 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2041 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2101 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2161 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2221 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     2281 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     2341 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     2401 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     2461 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     2521 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     2581 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     2641 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     2701 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     2761 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     2821 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     2881 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt
     2941 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     3001 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     3061 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     3121 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     3181 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     3241 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     3301 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     3361 gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt
     3421 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     3481 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     3541 acggccagtg agcgcgcctc gttcattcac gtttttgaac ccgtggagga cgggcagact
     3601 cgcggtgcaa atgtgtttta cagcgtgatg gagcagatga agatgctcga cacgctgcag
     3661 aacacgcagc tagattaacc ctagaaagat aatcatattg tgacgtacgt taaagataat
     3721 catgtgtaaa attgacgcat gtgttttatc ggtctgtata tcgaggttta tttattaatt
     3781 tgaatagata ttaagtttta ttatatttac acttacatac taataataaa ttcaacaaac
     3841 aatttattta tgtttattta tttattaaaa aaaacaaaaa ctcaaaattt cttctataaa
     3901 gtaacaaaac ttttatgagg gacagccccc ccccaaagcc cccagggatg taattacgtc
     3961 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
     4021 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
     4081 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
     4141 ggggaaaagg cctccacggc cactagtaaa cgagagacgt ttacggctag ctcagtccta
     4201 ggtatagtgc tagcaaagag gagaaatact agatggtgag caagggcgag gagctgttca
     4261 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     4321 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     4381 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     4441 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     4501 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     4561 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     4621 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     4681 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     4741 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     4801 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     4861 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     4921 tcactctcgg catggacgag tagtacaagc agataaaaaa aatccttagc tttcgctaag
     4981 gatgatttct cgtctcagct gggatccgat ttaaattcga attcgggccc gcggccgcac
     5041 tcaattctct agaaatattc tcgaggtttc tccggtgccc gtcagtgggc agagcgcaca
     5101 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
     5161 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     5221 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     5281 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
     5341 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
     5401 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
     5461 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
     5521 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
     5581 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
     5641 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
     5701 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
     5761 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     5821 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     5881 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     5941 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     6001 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     6061 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     6121 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     6181 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     6241 tcgtgaggcg ccaccatgaa aaagcctgaa ctcaccgcta cctctgtcga gaagtttctg
     6301 atcgaaaagt tcgacagcgt gtccgacctg atgcagctct ccgagggcga agaatctcgg
     6361 gctttcagct tcgatgtggg agggcgtgga tatgtcctgc gggtgaatag ctgcgccgat
     6421 ggtttctaca aagatcgcta tgtttatcgg cactttgcat ccgccgctct ccctattccc
     6481 gaagtgcttg acattgggga gttcagcgag agcctgacct attgcatctc ccgccgtgca
     6541 cagggtgtca ccttgcaaga cctgcctgaa accgaactgc ccgctgttct ccagcccgtc
     6601 gccgaggcca tggatgccat cgctgccgcc gatcttagcc agaccagcgg gttcggccca
     6661 ttcggacctc aaggaatcgg tcaatacact acatggcgcg atttcatctg cgctattgct
     6721 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc ctccgtcgcc
     6781 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg
     6841 cacgccgatt tcggctccaa caatgtcctg accgacaatg gccgcataac agccgtcatt
     6901 gactggagcg aggccatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg
     6961 aggccctggt tggcttgtat ggagcagcag acccgctact tcgagcggag gcatcccgag
     7021 cttgcaggat ctcctcggct ccgggcttat atgctccgca ttggtcttga ccaactctat
     7081 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg ctcagggtcg ctgcgacgca
     7141 atcgtccggt ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgctgcc
     7201 gtctggaccg atggctgtgt ggaagtgctc gccgatagtg gaaacagacg ccccagcact
     7261 cgtcctaggg caaaggatct gcagtaagtc gacaatcaac ctctggatta caaaatttgt
     7321 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     7381 ttaatgcctt tgtatcatgc gttaactaaa cttgtttatt gcagcttata atggttacaa
     7441 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7501 tggtttgtcc aaactcatca atgtatctta tcatgtctgg aattgactca aatgatgtca
     7561 attagtctat cagaagctca tctggtctcc cttccggggg acaagacatc cctgtttaat
     7621 atttaaacag cagtgttccc aaactgggtt cttatatccc ttgctctggt caaccaggtt
     7681 gcagggtttc ctgtcctcac aggaacgaag tccctaaaga aacagtggca gccaggttta
     7741 gccccggaat tgactggatt ccttttttag ggcccattgg tatggctttt tccccgtatc
     7801 cccccaggtg tctgcaggct caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt
     7861 gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg gctcggggat gcggggggag
     7921 cgccggaccg gagcggagcc ccgggcggct cgctgctgcc ccctagcggg ggagggacgt
     7981 aattacatcc ctgggggctt t
//
