LOCUS       THWPLZ_2_cyriB-m        6348 bp DNA     circular SYN 31-OCT-2025
DEFINITION  cyriB-mCherry.
ACCESSION   cyriB-mCherry
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 31, 2025 from SnapGene Viewer 7.2.1
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
COMMENT     Sequence Label: p17-mCherry-CYRI-B
FEATURES             Location/Qualifiers
     source          1..6348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             772..864
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    877..898
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        913..943
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    951..967
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     enhancer        1020..1399
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        1400..1603
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     intron          1739..1782
                     /label=CMV intron (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.2"
                     /note="/other=intron"
     promoter        1834..1852
                     /label=SP6 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1856..1893
                     /codon_start=1
                     /label=IgH signal sequence (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=27.3"
                     /note="/other=CDS"
                     /translation="TSTLPFSPQVST"
     misc_feature    1931..1981
                     /label=CYRI-B
     CDS             1988..2692
                     /codon_start=1
                     /label=mCherry
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.6"
                     /note="/other=CDS"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     misc_feature    2699..3619
                     /label=CYRI-B
     misc_feature    3623..3646
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     polyA_signal    3667..3801
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     promoter        3870..4227
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             4246..4400
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.1"
                     /note="/other=CDS"
                     /translation="HWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPNS
                     CAA*MA"
     misc_feature    complement(4406..4457)
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      4476..4856
                     /label=M13 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    complement(4932..4981)
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=35.5"
                     /note="/other=misc_feature"
     CDS             complement(5083..5118)
                     /codon_start=1
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.8"
                     /note="/other=CDS"
                     /translation="LARFGDDGENL"
     promoter        5213..5317
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             5318..6178
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgctacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga catgattacg aattaattcg agctcgcccg
     1021 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     1081 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1141 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1201 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1261 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     1321 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     1381 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     1441 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     1501 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     1561 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     1621 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
     1681 cagcctccgc ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca agagtgacgt
     1741 aagtaccgcc tatagagtct ataggcccac ccccttggct tcgttagaac gcggctacaa
     1801 ttaatacata accttatgta tcatacacat acgatttagg tgacactata gaataacatc
     1861 cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcacct cggttctatc
     1921 gattgaattc atggggaatc ttcttaaagt tttgacatgc acagaccttg agcaggggcc
     1981 actcgaggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     2041 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     2101 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     2161 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     2221 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     2281 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     2341 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     2401 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     2461 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     2521 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     2581 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     2641 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca aggctagcaa
     2701 ttttttcctt gattttgaaa atgcccagcc tacagagtct gagaaggaaa tttataatca
     2761 ggtgaatgta gtattaaaag atgcagaagg catcttggag gacttgcagt catacagagg
     2821 agctggccac gaaatacgag aggcaatcca gcatccagca gatgagaagt tgcaagagaa
     2881 ggcatggggt gcagttgttc cactagtagg caaattaaag aaattttacg aattttctca
     2941 gaggttagaa gcagcattaa gaggtcttct gggagcctta acaagtaccc catattctcc
     3001 cacccagcat ctagagcgag agcaggctct tgctaaacag tttgcagaaa ttcttcattt
     3061 cacactccgg tttgatgaac tcaagatgac aaatcctgcc atacagaatg atttcagcta
     3121 ttatagaaga acattgagtc gtatgaggat taacaatgta ccggcagaag gagaaaatga
     3181 agtaaataat gaattggcaa atcgaatgtc tttgttttat gctgaggcaa ctccaatgct
     3241 gaaaaccttg agtgatgcca caacaaaatt tgtatcagag aataaaaatt taccaataga
     3301 aaataccaca gattgtttaa gcacaatggc tagtgtatgc agagtcatgc tggaaacacc
     3361 ggaatacaga agcagattta caaatgaaga gacagtgtca ttctgcttga gggtaatggt
     3421 gggtgtcata atactctatg accacgtaca tccagtggga gcatttgcta aaacttccaa
     3481 aattgatatg aaaggttgta tcaaagttct taaggaccaa cctcctaata gtgtggaagg
     3541 tcttctaaat gctctcaggt acacaacaaa acatttgaat gatgagacta cctccaagca
     3601 aattaaatcc atgctgcaat aaggatcctc tagagtcgac ctgcagaagc ttggccgcca
     3661 tggcccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3721 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3781 gtatcttatc atgtctggat cgggaattaa ttcggcgcag caccatggcc tgaaataacc
     3841 tctgaaagag gaacttggtt aggtaccttc tgaggcggaa agaaccagct gtggaatgtg
     3901 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3961 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4021 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4081 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4141 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4201 ttttttggag gcctaggctt ttgcaaaaag ctgttaacag cttggcactg gccgtcgttt
     4261 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     4321 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     4381 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     4441 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     4501 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4561 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4621 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4681 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4741 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4801 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
     4861 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4921 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     4981 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     5041 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     5101 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     5161 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5221 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5281 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5341 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5401 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5461 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5521 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5581 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5641 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5701 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5761 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5821 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     5881 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5941 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6001 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6061 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6121 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6181 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6241 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6301 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
