LOCUS       MultiCAST_miniTn        2466 bp    DNA        circular     30-OCT-2025
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     oriT            247..355
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="oriT"
                     /locus_tag="RP4 oriT"
                     /label="RP4 oriT"
                     /ApEinfo_label="RP4 oriT"
                     /ApEinfo_fwdcolor="#fb027f"
                     /ApEinfo_revcolor="#fb027f"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(401..788)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /locus_tag="R6K gamma ori"
                     /label="R6K gamma ori"
                     /ApEinfo_label="R6K gamma ori"
                     /ApEinfo_fwdcolor="#cc66fe"
                     /ApEinfo_revcolor="#cc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(878..1002)
                     /locus_tag="CAST Tn6677 right end"
                     /label="CAST Tn6677 right end"
                     /ApEinfo_label="CAST Tn6677 right end"
                     /ApEinfo_fwdcolor="#66ccfe"
                     /ApEinfo_revcolor="#66ccfe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      810..857
                     /locus_tag="T7 terminator"
                     /label="T7 terminator"
                     /ApEinfo_label="T7 terminator"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2295..2439
                     /locus_tag="CAST Tn6677 left end"
                     /label="CAST Tn6677 left end"
                     /ApEinfo_label="CAST Tn6677 left end"
                     /ApEinfo_fwdcolor="#66ccfe"
                     /ApEinfo_revcolor="#66ccfe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(1887..1977)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /locus_tag="Pbla"
                     /label="Pbla"
                     /ApEinfo_label="Pbla"
                     /ApEinfo_fwdcolor="#fdcc65"
                     /ApEinfo_revcolor="#fdcc65"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2104..2138
                     /locus_tag="J23119 Promoter"
                     /label="J23119 Promoter"
                     /ApEinfo_label="J23119 Promoter"
                     /ApEinfo_fwdcolor="#fdcc65"
                     /ApEinfo_revcolor="#fdcc65"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2169..2191
                     /locus_tag="Fwd primer binding site"
                     /label="Fwd primer binding site"
                     /ApEinfo_label="Fwd primer binding site"
                     /ApEinfo_fwdcolor="#ff2600"
                     /ApEinfo_revcolor="#ff2600"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2241..2268
                     /locus_tag="Atypical repeat 2"
                     /label="Atypical repeat 2"
                     /ApEinfo_label="Atypical repeat 2"
                     /ApEinfo_fwdcolor="#8df900"
                     /ApEinfo_revcolor="#8df900"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2233..2240
                     /locus_tag="PacI recognition site"
                     /label="PacI recognition site"
                     /ApEinfo_label="PacI recognition site"
                     /ApEinfo_fwdcolor="#66fffe"
                     /ApEinfo_revcolor="#66fffe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2220..2225
                     /locus_tag="SpeI recognition site"
                     /label="SpeI recognition site"
                     /ApEinfo_label="SpeI recognition site"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2192..2219
                     /locus_tag="Atypical repeat 1"
                     /label="Atypical repeat 1"
                     /ApEinfo_label="Atypical repeat 1"
                     /ApEinfo_fwdcolor="#8df900"
                     /ApEinfo_revcolor="#8df900"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    2275..2294
                     /locus_tag="Rev primer binding site"
                     /label="Rev primer binding site"
                     /ApEinfo_label="Rev primer binding site"
                     /ApEinfo_fwdcolor="#ff2600"
                     /ApEinfo_revcolor="#ff2600"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     CDS             complement(1026..1886)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /locus_tag="CarbR/AmpR"
                     /label="CarbR/AmpR"
                     /ApEinfo_label="CarbR/AmpR"
                     /ApEinfo_fwdcolor="#21fe80"
                     /ApEinfo_revcolor="#21fe80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 TCGGTGTAGC TCTTTGGCAT CGTCTCTCGC CTGTCCCCTC AGTTCAGTAA TTTCCTGCAT
       61 TTGCCTGTTT CCAGTCGGTA GATATTCCAC AAAACAGCAG GGAAGCAGCG CTTTTCCGCT
      121 GCATAACCCT GCTTCGGGGT CATTATAGCG ATTTTTTCGG TATATCCATC CTTTTTCGCA
      181 CGATATACAG GATTTTGCCA AAGGGTTCGT GTAGACTTTC CTTGGTGTAT CCAACGGCGT
      241 CAGCCGGGCA GGATAGGTGA AGTAGGCCCA CCCGCGAGCG GGTGTTCCTT CTTCACTGTC
      301 CCTTATTCGC ACCTGGCGGT GCTCAACGGG AATCCTGCTC TGCGAGGCTG GCCGGCTACC
      361 GCCGGCGTAA CAGATGAGGG CAAGCGGGGT ACAATTCCCA TGTCAGCCGT TAAGTGTTCC
      421 TGTGTCACTC AAAATTGCTT TGAGAGGCTC TAAGGGCTTC TCAGTGCGTT ACATCCCTGG
      481 CTTGTTGTCC ACAACCGTTA AACCTTAAAA GCTTTAAAAG CCTTATATAT TCTTTTTTTT
      541 CTTATAAAAC TTAAAACCTT AGAGGCTATT TAAGTTGCTG ATTTATATTA ATTTTATTGT
      601 TCAAACATGA GAGCTTAGTA CGTGAAACAT GAGAGCTTAG TACGTTAGCC ATGAGAGCTT
      661 AGTACGTTAG CCATGAGGGT TTAGTTCGTT AAACATGAGA GCTTAGTACG TTAAACATGA
      721 GAGCTTAGTA CGTGAAACAT GAGAGCTTAG TACGTACTAT CAACAGGTTG AACTGCTGAT
      781 CTTCAGATCC TCTACCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG
      841 TCTTGAGGGG TTTTTTGCTG AAACCTCAGG CATTTGTTGT TGATACAACC ATAAAATGAT
      901 AATTACACCC ATAAATTGAT AATTATCACA CCCATAAATT GATATTGCCT CTTCATGGTC
      961 TAAACTTCAG TAAGTTTACG ACATTTTCCT CGAGGTCATT TCCGGGGATC CTTACCAATG
     1021 CTTAATTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA
     1081 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
     1141 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA
     1201 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
     1261 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG
     1321 CGCAACGTTG TTGCCATTGC TGCAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT
     1381 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
     1441 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA
     1501 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC
     1561 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG
     1621 AGTTGCTCTT GCCCGGCGTC AACACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA
     1681 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
     1741 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC
     1801 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG
     1861 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
     1921 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAAAG
     1981 AGTTTGACGG ATGGCCTTTT TGCGTTTCTA TGGATAACCG TATTACCGCC TTTGAGTGAG
     2041 CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGACACaa
     2101 gctttgacag ctagctcagt cctaggtata atactagcgt cgacAGGATC CGAATTCGAG
     2161 CTCGGTATGA TGGGTTAAAA AGGATCGATC CTCATTACTA CTGCAAAGTA GCTGATAACa
     2221 ctagtCCGAC TAttaattaa CTTTACTGCT GAATAAGTAG ATAACTACCA ATTCTTTCTT
     2281 GCCGCCAAGG ATCTCTTACT GCAGTAGTTT TGCTGAAATA CTCGATTCAC AAAAATATCA
     2341 ACTTATGGTT GTTTTGTGAG ATATCAATAT ATGGTTGTTT TGTGGTTAAG TTGCTGATTA
     2401 TAAATAATTA TTAAATATCA CTTTATGGTT GCATCAACAG GTACCGAGCT CGAATTCACT
     2461 GGCCGT     
//
