LOCUS       Exported                9658 bp ds-DNA     circular UNA 04-NOV-2025
DEFINITION  Gibson/Homology Cloning ligation product.
ACCESSION   urn.local.hongyue.1c-juw4zr4
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 5, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9658
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     gene            882..2028
                     /label=Ac_5_flank
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=Ac_5_flank"
                     /note="/Transferred_Similarity=100.00%"
     gene            2039..2072
                     /label=loxP
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=loxP"
                     /note="/Transferred_Similarity=100.00%"
     promoter        3228..3328
                     /label=PH prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=PH prom"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     3258..3292
                     /label=oHoY104_pAC8_F
     misc_feature    3341..3370
                     /label=StrepII tag
                     /note="/created_by=User"
     misc_feature    3380..3424
                     /label=BirA
                     /note="/created_by=User"
     misc_feature    3443..6331
                     /label=IDT_DCAF5-FL-no tag (concatenated sequence 2)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument:
                     urn:local:hongyue:8j-d09vx3v"
     misc_feature    3443..3470
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    3443..3463
                     /label=TEV
                     /note="/created_by=User"
     misc_feature    3443..3454
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    3473..6298
                     /label=DCAF5-FL
                     /note="/created_by=hongyue"
     misc_feature    3473..6298
                     /label=ORF 1 (frame 3)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=3"
                     /note="/created_by=ORF Finder"
                     /note="/modified_by=hongyue"
     misc_feature    complement(3602..3736)
                     /label=ORF 29 (frame 1)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=1"
                     /note="/created_by=ORF Finder"
     misc_feature    3678..3848
                     /label=ORF 2 (frame 1)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=1"
                     /note="/created_by=ORF Finder"
     misc_feature    complement(3822..3956)
                     /label=ORF 28 (frame 3)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=3"
                     /note="/created_by=ORF Finder"
     misc_feature    complement(3953..4135)
                     /label=ORF 27 (frame 1)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=1"
                     /note="/created_by=ORF Finder"
     misc_feature    complement(4042..4203)
                     /label=ORF 26 (frame 2)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=2"
                     /note="/created_by=ORF Finder"
     misc_feature    complement(5320..5430)
                     /label=ORF 19 (frame 2)
                     /label=nonstandard type:  ORF
                     /note="/Genetic_code=Standard"
                     /note="/Reading_frame=2"
                     /note="/created_by=ORF Finder"
     misc_feature    6299..6301
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     misc_feature    join(6303..9658,1..3470)
                     /label=pHoY105_pMH394_pAC8RedNK_Strep_BirA_short-linke
                     /label=pHoY105_pMH394_pAC8RedNK_Strep_BirA_short-linker-T
                     EV (digested by NotI, KpnI) Fragment 2 (concatenated 
                     sequence 1)
                     /note="Geneious type: Concatenated sequence"
                     /note="/Source=referenceToDocument:
                     urn:local:hongyue:7d-d09vd1p"
     misc_feature    6303..6331
                     /label=Ligation
                     /label=nonstandard type:  ligation
     misc_feature    6303..6330
                     /label=AAAATCTGTATTTTCAGGGCGGTAGCGGTGGTGGTCTGAATGATATA
                     /label=AAAATCTGTATTTTCAGGGCGGTAGCGGTGGTGGTCTGAATGATATATTC 
                     GAGGCCCAGAAAATAGAATGGCATGAATCCATGGTCTCTATGGATTGGAGCCATCCAC 
                     AGTTTGAGAAATAGGGTACCCCCGGACGGCC
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=AAAATCTGTATTTTCAGGGCGGTAGCGGTGGTGGTC
                     TGAAT 
                     GATATATTCGAGGCCCAGAAAATAGAATGGCATGAATCCATGGTCTCTATGGATTGGA 
                     GCCATCCACAGTTTGAGAAATAGGGTACCCCCGGACGGCC"
     gene            6338..7771
                     /label=Ac_3_flank
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=Ac_3_flank"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     complement(6364..6394)
                     /label=oHoY105_pAC8_R
     misc_feature    7859..8165
                     /label=f1 origin
                     /note="Geneious type: origin of replication"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=f1 origin"
                     /note="/Transferred_Similarity=100.00%"
     promoter        8614..8642
                     /label=amp prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=amp prom"
                     /note="/Transferred_Similarity=100.00%"
ORIGIN
        1 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
       61 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      121 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      181 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
      241 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      301 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      361 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      421 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      481 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      541 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      601 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      661 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      721 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
      781 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
      841 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gaacggctcc gcccactatt
      901 aatgaaatta aaaattccaa ttttaaaaaa cgcagcaaga gaaacatttg tatgaaagaa
      961 tgcgtagaag gaaagaaaaa tgtcgtcgac atgctgaaca acaagattaa tatgcctccg
     1021 tgtataaaaa aaatattgaa cgatttgaaa gaaaacaatg taccgcgcgg cggtatgtac
     1081 aggaagaggt ttatactaaa ctgttacatt gcaaacgtgg tttcgtgtgc caagtgtgaa
     1141 aaccgatgtt taatcaaggc tctgacgcat ttctacaacc acgactccaa gtgtgtgggt
     1201 gaagtcatgc atcttttaat caaatcccaa gatgtgtata aaccaccaaa ctgccaaaaa
     1261 atgaaaactg tcgacaagct ctgtccgttt gctggcaact gcaagggtct caatcctatt
     1321 tgtaattatt gaataataaa acaattataa atgctaaatt tgttttttat taacgataca
     1381 aaccaaacgc aacaagaaca tttgtagtat tatctataat tgaaaacgcg tagttataat
     1441 cgctgaggta atatttaaaa tcattttcaa atgattcaca gttaatttgc gacaatataa
     1501 ttttattttc acataaacta gacgccttgt cgtcttcttc ttcgtattcc ttctcttttt
     1561 catttttctc ctcataaaaa ttaacatagt tattatcgta tccatatatg tatctatcgt
     1621 atagagtaaa ttttttgttg tcataaatat atatgtcttt tttaatgggg tgtatagtac
     1681 cgctgcgcat agtttttctg taatttacaa cagtgctatt ttctggtagt tcttcggagt
     1741 gtgttgcttt aattattaaa tttatataat caatgaattt gggatcgtcg gttttgtaca
     1801 atatgttgcc ggcatagtac gcagcttctt ctagttcaat tacaccattt tttagcagca
     1861 ccggattaac ataactttcc aaaatgttgt acgaaccgtt aaacaaaaac agttcacctc
     1921 ccttttctat actattgtct gcgagcagtt gtttgttgtt aaaaataaca gccattgtaa
     1981 tgagacgcac aaactaatat cacaaactgg aaatgtctat caatatatag ttgctgatat
     2041 aacttcgtat agcatacatt atacgaagtt atctggttga aacgtacccg tagtggctat
     2101 ggcagggctt gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
     2161 ttggggtggg gaaaaggaag aaacgcgggc gtattggtcc caatggggtc tcggtggggt
     2221 atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     2281 cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct
     2341 ccttccgtgt ttcagttagc ctcccccatc tcccgctatt actcgagtca ctgggagccg
     2401 gagtggcggg cctcggcgtg ctcgtactgc tccaccacgg tgtagtcctc gttgtggttg
     2461 gtgatgtcca gcttggagtc cacgtagtgg ttgccgggca gctgcacggg cttcttggcc
     2521 ttgtacacgg tcttgaagtc gcaggtgtag tggccgccgt ccttcagctt cagggcgtgg
     2581 gagatctcgc ccttcagcac gccgtcctgg gggtacagct tctcggtgga gggctcccag
     2641 ccggcagtct tcttctgcat tacggggccg tcggcgggga agttcacgcc cttgaacttc
     2701 accttgtaga tgaaggtgcc gtcctgcagg gaggagtcct gctgcacctc caccacgccg
     2761 ccgtcctcga agttcatgga gcgctcccag gtgaagccct cggggaagga cagcttcatg
     2821 tagtcgggga tgtcggcggg gtgcttcacg taggccttgg agccgtactg gaactggggg
     2881 gacaggatgt cccaggcgaa gggcaggggg ccgcccttgg tcacctgcag cttggcggtc
     2941 tgggtgccct cgtagggctt gccctcgccc tcgccctcga tctcgaagta gtggccgttc
     3001 acggagccct ccatgcgcac cttgaactgc atgaactcct tgatgacgtc ctcggtgttg
     3061 tccatggtag agtgattgta aataaaatgt aatttacagt atagtatttt aattaatata
     3121 caaatgattt gataataatt cttatttaac tataatatat tgtgttgggt tgaattaaag
     3181 gtccgtatac tagtatcgat tcgcgaccta ctccggaata ttaatagagt tgctgatatc
     3241 atggagataa ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta
     3301 acagttttgt aataaaaaaa cctataaata cggatctacc atggactgga gccacccgca
     3361 gttcgaaaaa tccgcggtcg gcctgaacga catcttcgag gctcagaaaa tcgaatggca
     3421 cgaaggtgga ggaggttctg gagagaacct ctacttccag ggaggcggcc gcatgaagag
     3481 gcgagctggt ctgggcggtt ccatgagatc tgttgttggc tttctctctc aacgtggact
     3541 gcacggagat ccactgttga cgcaagactt ccaacgtcgt cgattgaggg gttgccgtaa
     3601 cctatataag aaggatttgc ttggtcattt cggctgcgtg aacgcaatcg aatttagcaa
     3661 taacggtggt caatggcttg tcagcggtgg agatgaccgc cgtgtgttgc tctggcacat
     3721 ggaacaggct atccattcac gcgtcaagcc cattcagctt aaaggcgaac accattcaaa
     3781 cattttctgc ctcgccttta acagcggaaa cacgaaggta tttagcggcg gcaacgatga
     3841 gcaagtgata ctacacgacg ttgaatcatc agaaactctt gacgtctttg ctcacgaaga
     3901 tgcagtttac ggtctgtcag tgtcgcccgt gaacgataac atctttgctt cgtcaagcga
     3961 cgacggtagg gttttgattt gggacatccg tgagtcgcca cacggcgagc ccttctgtct
     4021 cgctaactac ccatccgctt ttcacagtgt catgttcaat ccagtcgaac ctagattgct
     4081 ggctactgct aatagtaaag agggagtggg cctctgggat atccgtaaac cgcagagttc
     4141 cttgcttcga tacggtggca acctgagtct acaatccgct atgtctgtcc gttttaatag
     4201 caacggaact caattgctcg ctctccgaag acgccttcca ccggtccttt atgacatcca
     4261 ctcgcgcctg ccggtgttcc aattcgataa ccagggttac ttcaattcct gcaccatgaa
     4321 aagttgctgc tttgcgggcg accgtgatca atacattctg tctggatcgg acgattttaa
     4381 cctctatatg tggcgcatac cagccgaccc ggaagccggt ggtatcggac gcgtagttaa
     4441 cggagctttt atggtcctga aaggccaccg ttccattgtt aaccaagtgc gtttcaaccc
     4501 tcacacgtac atgatctgca gctctggagt cgagaaaatt atcaaaatat ggtcccccta
     4561 caagcaacca ggttgcaccg gtgacctgga tggcaggatc gaagacgatt cacgctgttt
     4621 gtacacccat gaagaataca tttcccttgt actcaactca ggctcaggcc tgtcccacga
     4681 ctacgcgaac cagtcggtgc aggaagaccc ccgcatgatg gccttcttcg acagcctagt
     4741 caggcgcgag atagagggtt ggtcatctga ttctgactcc gacctatctg agtcaaccat
     4801 tctccaactg cacgccggcg tgtcagagcg atctggttac acggattctg aatcctcggc
     4861 gtccctgcct aggtctccac cgccaacagt ggacgaatct gctgacaatg cgttccactt
     4921 gggtccactg cgcgtcacaa caactaacac tgtagcttcg actccaccta ctccgacgtg
     4981 cgaagacgct gcgtcccgtc aacaaaggtt gtccgcgctg agacgatacc aggacaagag
     5041 attgcttgcg ttgtcgaacg agagtgacag tgaagaaaac gtgtgtgaag tcgaactgga
     5101 cacagatctc ttcccccgcc cgaggtcccc ctcgccagaa gacgaatcat catcgtcctc
     5161 gtcgtcttcc agcagcgagg acgaggaaga actaaacgaa agaagggcca gcacatggca
     5221 gcgcaacgcc atgaggcgta ggcaaaagac aacgagggaa gacaaacctt cagcgccgat
     5281 aaaaccaacc aacacttaca tcggagaaga taactatgat tacccacaga tcaaagttga
     5341 tgacctgtca tcatcgccaa ccagctcacc cgaaagatcc actagtactc tagagatcca
     5401 acctagcaga gccagtccaa cttcggacat tgagtcggtt gaaaggaaga tctacaaagc
     5461 ctataagtgg ttgcgatata gttatatttc ctactctaac aacaaggacg gtgaaacctc
     5521 tttggtcaca ggcgaagcag acgagggtcg tgccggaacc tctcacaagg acaaccccgc
     5581 tccttcatcc tcgaaagagg cttgcttgaa catcgccatg gcccaacgta accaagatct
     5641 tccccctgag ggctgttcca aggacacgtt caaggaagaa acaccccgca cgccgtccaa
     5701 cggacctggt catgagcatt ccagtcacgc gtgggcggaa gttccagagg gaacatctca
     5761 agatacaggt aattcgggtt ccgtcgagca cccgttcgag actaagaagc taaacggtaa
     5821 ggctctctct tctcgcgcgg aagagccgcc gtcaccccca gtgcctaagg cgtcgggtag
     5881 cacactcaac tcaggctctg gaaattgccc taggacacaa agcgacgaca gcgaagagag
     5941 gtccctggag acaatctgcg cgaaccacaa caacggaaga ctgcaccccc gtccacccca
     6001 cccccacaat aacggtcaaa acctgggaga actagaggtt gtagcctatt caagcccagg
     6061 tcactctgac accgatcgcg acaatagctc tctgactggt actctcttgc ataaagattg
     6121 ctgtggctct gagatggcct gtgagactcc caacgccggt actagggaag atccaacaga
     6181 cactcccgcc acagactcat cacgtgcagt gcatggccac tcaggactta agagacagcg
     6241 tatagagctt gaggacaccg actccgaaaa ctcgtcctct gaaaagaaac tcaagacctg
     6301 aggtaccccc ggacggccgc ttaattaatt gatccgggtt attagtacat ttattaagcg
     6361 ctagattctg tgcgttgttg atttacagac aattgttgta cgtattttaa taattcatta
     6421 aatttataat ctttagggtg gtatgttaga gcgaaaatca aatgattttc agcgtcttta
     6481 tatctgaatt taaatattaa atcctcaata gatttgtaaa ataggtttcg attagtttca
     6541 aacaagggtt gtttttccga accgatggct ggactatcta atggattttc gctcaacgcc
     6601 acaaaacttg ccaaatcttg tagcagcaat ctagctttgt cgatattcgt ttgtgttttg
     6661 ttttgtaata aaggttcgac gtcgttcaaa atattatgcg cttttgtatt tctttcatca
     6721 ctgtcgttag tgtacaattg actcgacgta aacacgttaa ataaagcttg gacatattta
     6781 acatcgggcg tgttagcttt attaggccga ttatcgtcgt cgtcccaacc ctcgtcgtta
     6841 gaagttgctt ccgaagacga ttttgccata gccacacgac gcctattaat tgtgtcggct
     6901 aacacgtccg cgatcaaatt tgtagttgag ctttttggaa ttatttctga ttgcgggcgt
     6961 ttttgggcgg gtttcaatct aactgtgccc gattttaatt cagacaacac gttagaaagc
     7021 gatggtgcag gcggtggtaa catttcagac ggcaaatcta ctaatggcgg cggtggtgga
     7081 gctgatgata aatctaccat cggtggaggc gcaggcgggg ctggcggcgg aggcggaggc
     7141 ggaggtggtg gcggtgatgc agacggcggt ttaggctcaa atgtctcttt aggcaacaca
     7201 gtcggcacct caactattgt actggtttcg ggcgccgttt ttggtttgac cggtctgaga
     7261 cgagtgcgat ttttttcgtt tctaatagct tccaacaatt gttgtctgtc gtctaaaggt
     7321 gcagcgggtt gaggttccgt cggcattggt ggagcgggcg gcaattcaga catcgatggt
     7381 ggtggtggtg gtggaggcgc tggaatgtta ggcacgggag aaggtggtgg cggcggtgcc
     7441 gccggtataa tttgttctgg tttagtttgt tcgcgcacga ttgtgggcac cggcgcaggc
     7501 gccgctggct gcacaacgga aggtcgtctg cttcgaggca gcgcttgggg tggtggcaat
     7561 tcaatattat aattggaata caaatcgtaa aaatctgcta taagcattgt aatttcgcta
     7621 tcgtttaccg tgccgatatt taacaaccgc tcaatgtaag caattgtatt gtaaagagat
     7681 tgtctcaagc tcggatcgat cccgcacgcc gataacaagc cttttcattt ttactacagc
     7741 attgtagtgg cgagacactt cgctgtcgtc gcctgatgcg gtattttctc cttacgcatc
     7801 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     7861 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     7921 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     7981 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     8041 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     8101 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     8161 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
     8221 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     8281 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     8341 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     8401 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     8461 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     8521 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     8581 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     8641 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     8701 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8761 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8821 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8881 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8941 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     9001 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     9061 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     9121 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     9181 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     9241 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     9301 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     9361 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     9421 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     9481 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     9541 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     9601 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaa
//
