LOCUS       Exported                4567 bp ds-DNA     circular SYN 05-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 5, 2025 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          2194..2220
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          2197..2220
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          2215..2220
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          2215..2220
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(220..322)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene"
     rep_origin      complement(848..1410)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the colE1 origin."
     misc_feature    1415..1449
                     /label=PJ23100
     misc_feature    1450..1476
                     /label=RBS
     CDS             1477..2190
                     /codon_start=1
                     /label=dpGFP
                     /translation="MSKSNELFTGVVPILVELDGDVNGHKFSVSGEGVGDATYGKLTLK
                     FICTTGKLPVPWPTLVTSLTYGVQCFSHYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGNTLVNRIELKGIDFKEDGNILGHKLQYNYNSHNVYIMADKQKNGIKG
                     NFKIRHNMEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSILSKDPNEHRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    2215..2461
                     /label=rrnBT
     CDS             complement(join(4127..4567,1..219))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
       61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
      961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     1381 aaaacgatct caagaagatc atcttattaa tcagttgacg gctagctcag tcctaggtac
     1441 agtgctagcc aattcattaa agaggagaaa ggatccatga gcaagagcaa cgagctgttc
     1501 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     1561 gtgtccggcg agggcgtggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1621 accaccggca agctgcccgt gccctggccc accctcgtga ccagcctgac ctacggcgtg
     1681 cagtgcttca gccactaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1741 cccgagggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1801 cgcgccgagg tgaagttcga gggcaacaca ctagtgaacc gcatcgagct gaagggcatc
     1861 gacttcaagg aggacggcaa catcctgggg cacaagctgc agtacaacta caacagccac
     1921 aacgtctata tcatggccga caagcagaag aacggcatca agggcaactt caagatccgc
     1981 cacaacatgg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     2041 ggcgacggcc ccgtgttgct gcccgacaac cactacctga gcacccagtc catcctgagc
     2101 aaagacccca acgagcaccg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     2161 atcactcacg gcatggacga gctgtacaag tgataatcta gaaagcttct cgagaggcat
     2221 caaataaaac gaaaggctca gtcggaagac tgggcctttc gttttatctg ttgtttgtcg
     2281 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa
     2341 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag
     2401 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttcctgt cgtcatatct
     2461 aggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
     2521 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
     2581 caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
     2641 atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
     2701 actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc
     2761 gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt
     2821 atgattcttc tcacttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg
     2881 caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta
     2941 acttcgatca ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg
     3001 aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt
     3061 cgcggtgcat ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg
     3121 gattcaccac tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa
     3181 ccaacccttg gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca
     3241 tctcgggcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga
     3301 cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc
     3361 gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct
     3421 tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc ccctacgtgc tgctgaagtt
     3481 gcccgcaaca gagagtggaa ccaaccggtg ataccacgat actatgactg agagtcaacg
     3541 ccatgagcgg cctcatttct tattctgagt tacaacagtc cgcaccgctg ccggtagctc
     3601 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
     3661 gcaactcgta ggacaggtgc cggcagcgcc caacagtccc ccggccacgg ggcctgccac
     3721 catacccacg ccgaaacaag cgccctgcac cattatgttc cggatctgca tcgcaggatg
     3781 ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctaac cgtttttatc
     3841 aggctctggg aggcagaata aatgatcata tcgtcaatta ttacctccac ggggagagcc
     3901 tgagcaaact ggcctcaggc atttgagaag cacacggtca cactgcttcc ggtagtcaat
     3961 aaaccggtaa accagcaata gacataagcg gctatttaac gaccctgccc tgaaccgacg
     4021 accgggtcga atttgctttc gaatttctgc cattcatccg cttattatca cttattcagg
     4081 cgtagcaacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct
     4141 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac
     4201 aaacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat
     4261 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa
     4321 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt
     4381 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa
     4441 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat
     4501 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg
     4561 ccatacg
//
