LOCUS       mEmerald-C1-Myo1e                 8013 bp    DNA     circular SYN 13-MAR-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(7098..7892)
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(4459..5175)
                     /note="pLannotate"
                     /label="mEmerald"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      404..832
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(join(7927..8013,1..271))
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(5424..5727)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(5220..5423)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1120..4443)
                     /note="pLannotate"
                     /label="MYO1E"
                     /database="swissprot"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    complement(866..987)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(273..377)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      complement(5902..6490)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(6819..6866)
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(6925..7077)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1010..1094)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.0"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
       61 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
      121 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
      181 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
      241 cacattccac agctggttct ttccgcctca ggactcttcc tttttcaata ttattgaagc
      301 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
      361 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc
      421 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      481 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      541 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      601 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      661 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      721 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
      781 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
      841 atattaacgc ttacaattta cgcgttaaga tacattgatg agtttggaca aaccacaact
      901 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
      961 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     1021 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     1081 gctgattatg atcagttatc tagatccggt ggatcctcag atcttggtca catagttgtt
     1141 ggggaacagg ccctgcttgc ctcgtagtcg acccgtccac cagccagaag gatcttcttt
     1201 gataatatca ataatgtcat tggcattaaa gctgagttcg tctgtgtcct gagcgtcata
     1261 ggcatacaaa gccttgcact gtggcacctg aggcttgggc ttgggctggg gcttgggtct
     1321 gccccctgct gggggaggcc gactggttgt ttgtctcctg acccctgcag ctccctggtc
     1381 cgggaccttg aggaaatcca ggctctctgg cgtctgtgac actcggtctg aactggtaga
     1441 ctgctgccga ggcaagggcg ggcgggccat ggaggtgtac aggcttttct gattggacct
     1501 ctggcttcca ggagcatggg gatatggcac atactggttt ctgatgactc cgttctgatg
     1561 gtatcctggg ggaggagggg cagctctcac tgggtagttg gcattttgag tcccactgga
     1621 ataacctgta ttttgggtag tgttccttct ggtaggacgg gagttcttgg gcagtccagg
     1681 tccgatgctg acctgcagca ctttgttact gggcttgagg acagccaggt ccccaaaccc
     1741 ttggtggaac tgcacttgcc gggagccccc cgcactccag gggccccagt tttccttttt
     1801 caacttcagt tcaagcgtat tgctgaattt cagaggtagt tgcttctggg tcttctcctc
     1861 gtaacgcttt gctaagaggc ttaggaattc agttttgaag acagattcaa gcaaactgtc
     1921 atactcttgc tcatggagaa taaaaatgtc atcctgcata gtactgaggg acacagacaa
     1981 gatccgttct atctcgattt tccgcttcag gacttctttc accaggccct tgtctgggcc
     2041 ctgtttgact ttttctcgtc cgattaagta caagcacttt ggggtaagga gcaggtctcg
     2101 ctttacaccc ttgaacctcc tgtcatactt ggtgactgtg tctgcgaaat caatcctctc
     2161 cctcttgccc acgaactgct ggagttctgg gtgctcttcc atcccaatat aatcccctat
     2221 aaagttcctg ttaatactgt ttctccttct ctccttcttg ttcaataaga ggtctgaggc
     2281 ttcttctctc atttgaacgt atttcttccg ggccacgaat ttcctccatg atttctgtat
     2341 cactcgagca tacccatcat actttctctc tctcatctct tctaaaagaa atagagactc
     2401 gggggctttg atgaacactt tactcctccc cagctggaac tggtcgctgt ccatgttgac
     2461 cgactgcagc aggtgcagga cgccttgctt ctcctctccc tgccaagaag gccaggtggc
     2521 tttggtcaga atggcgtacc tctgtaggaa tttttggaag atgcgccgat aggcatagcc
     2581 agctcttctc actcgaatgt tctctttcag acccaaatat tcgacttgat gctttaccct
     2641 gctttcctcc cagtctctgg gcttcttggt ttcgtttggc ttgatgcagc gaatgtagtg
     2701 gggcgtacat ttcatcaggg tgctcacaag gtcattggct tgtttcttta ttttgcttcc
     2761 ggcagtagtt gggcgccctt tcttgtcagc ctgcagattt tccggaaata aagactttat
     2821 gaaaggcagc tcgctgctct gcataagctc gatgagatcc ataaaaagca catcccggtt
     2881 cctttcacaa aagccatcca tgtcatagga taccttccca gcataatgat gaatgatgaa
     2941 gccttggttc caactgttga agtgctcatg actcccaatc tgcatctgaa gtttctggag
     3001 cagcgtctga tctgccccct cacccaccgc atgcatcgtg gcgcacacgt catccaggat
     3061 gctcatgatg ccaggagggt tcactttgtt ctctatgagg tcacatacga ttttattatt
     3121 aaagtactca atgggtgtcc atcttattcc ctcttgaaca tattcttcct gttctgcctt
     3181 taatgtcagt tcaataaaaa tctgctgcag tttttcatta acaaaattga tacaaaactg
     3241 ttcaaagcca tttttctgga atatttcaaa gccatagatg tctaggacgc caatgttgta
     3301 ttcttcatgg tctttctcca tggctttatt gatggaatct accaagaaat caaagacccg
     3361 ggcgtgcagg gccttggcga gcgcatcccg ggtgtaacag gcctgctcta cgttgagggt
     3421 cacgtggatg gattcagatt tgcctcccca cttgctatcc atctgccggc ttgttagctt
     3481 ttctttcaac cggtcctggt ttatccctag cagatatgca ggaaaagcta aaaactcttc
     3541 actctccaca gccgcgtagt tgccaacttc tttgaagctg atgtttccca ggtggagaat
     3601 acccgccact atctgcaaca ccagcgtttg ctcttctgca aagatcccaa tcacattcat
     3661 ggcgtgcaga gtttcctgaa actcccgcct gtcgtcaatg tcatcaacct tgtatgagcc
     3721 cgagaggctc aggtagtaat aatagtccat gctggtgatg ccaaggctgt gtttctgctc
     3781 tgcagaggcg ccctcgatga gctggtaaaa tatgtgaaaa ctccgctctc ctgggttcct
     3841 catcaccacc ctagattttt ccagaaggaa gttggagatc tttccaccat ctggttcccc
     3901 acctggactg aactggattt caaagtattt tccaaatcgg ctggagttgt tgttccggac
     3961 ggtcttggcg ttcccgaagg cctccagcag cgggttggac tgcaggataa tgtccttcac
     4021 gtgctggact ttggtccctc ctccagacac tctggagatg tagctcatga tatatttggc
     4081 agccactgtt tttccagcac cactttcacc actgataatg acgcactggt tctctctgtc
     4141 aatgatcatg tttctgtaca tattatctgc aagggcatag atatgtggtg ggttttcata
     4201 ctgtgccgct ccttggtaca tttcaatttc cttttcccca aaatatggca tctgcttgaa
     4261 agggttgact gagattaata cagatcctat atatgtaaaa atgtagtcat ccatgtatct
     4321 cttcttcaga ttctccacga tggagttctc tgtgatcttg gacagtagca ccatgtcgtc
     4381 cacaccactg tgcttgacat tgtggctttg ccagtggtac tggtagacac ctttgcttcc
     4441 catagatctg agtccggact tgtacagctc gtccatgccg agagtgatcc cggcggcggt
     4501 cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga
     4561 ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt
     4621 ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc gggtcttgaa
     4681 gttcaccttg atgccgttct tctgcttgtc ggcggtgata tagaccttgt ggctgttgta
     4741 gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag
     4801 ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt
     4861 gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt
     4921 gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg aagcactgca cgccgtaggt
     4981 caaggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt
     5041 cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg
     5101 gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc
     5161 gcccttgctc accatggtgg cgaccggtag cgctagcgga tctgacggtt cactaaacca
     5221 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
     5281 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
     5341 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
     5401 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
     5461 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     5521 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     5581 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     5641 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     5701 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     5761 cccgtaattg attactatta ataactaatg catggcggta atacggttat ccacagaatc
     5821 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     5881 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     5941 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6001 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6061 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6121 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6181 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6241 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6301 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     6361 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6421 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6481 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6541 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6601 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaacctg aggctatggc
     6661 agggcctgcc gccccgacgt tggctgcgag ccctgggcct tcacccgaac ttggggggtg
     6721 gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa tggggtctcg gtggggtatc
     6781 gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc caacaccgtg
     6841 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt
     6901 ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac tccagcatga gatccccgcg
     6961 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
     7021 ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc
     7081 gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     7141 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     7201 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
     7261 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     7321 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg
     7381 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     7441 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     7501 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     7561 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     7621 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     7681 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg
     7741 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     7801 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     7861 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
     7921 tcgatctttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     7981 agaggccgag gcggcctcgg cctctgcata aat
//
