LOCUS       mEmerald-C1-Myo1f#2                 7983 bp    DNA     circular SYN 02-MAY-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             complement(1120..4413)
                     /note="pLannotate"
                     /label="MYO1F"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(7068..7862)
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(4429..5145)
                     /note="pLannotate"
                     /label="mEmerald"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      404..832
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(join(7897..7983,1..271))
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(5394..5697)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(5190..5393)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(866..987)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(273..377)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      complement(5872..6460)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(6789..6836)
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="98.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(6895..7047)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="40.5"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1010..1094)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="4.0"
                     /fragment="True"
                     /other="CDS"
     polyA_signal    6682..6698
                     /note="pLannotate"
                     /label="bGH poly(A) signal (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.2"
                     /fragment="True"
                     /other="polyA_signal"
ORIGIN
        1 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
       61 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
      121 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
      181 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
      241 cacattccac agctggttct ttccgcctca ggactcttcc tttttcaata ttattgaagc
      301 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
      361 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc
      421 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      481 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      541 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      601 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      661 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      721 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
      781 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
      841 atattaacgc ttacaattta cgcgttaaga tacattgatg agtttggaca aaccacaact
      901 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
      961 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     1021 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     1081 gctgattatg atcagttatc tagatccggt ggatcctcag atcttctcca cgtagtttcc
     1141 tgggaaaagg ccctcctggc cgtgaagccg gcccttccac cagcccgagg gatcttccat
     1201 gaggatctca atgacctcgt tcacgttgaa gctcagctcg tccacatctt ggcccacgta
     1261 ctggtatagg gcccggcacc tgggaccatg tgtccgaggc tggggcttgg gtcggcccac
     1321 accaggcact ggccgttgcc ccacgctgcg cttcctctgc atgccggcca tgccctggtc
     1381 aggcacgttg aggaattctg tgttgtgctc tgagggcgga cgtgcccggg gtcgtctgct
     1441 ggctcccagg gatgtggacg gagggccccg gggaggcctg tgggtgcccc ctccagacat
     1501 gatctccagg ggcagggggc cccctctggc agaggggggc accccattgc gatccatgcc
     1561 tctggggggc gcaggggccg cccgggtagg ggcttgggac gacctccgag gttttccctt
     1621 ggccattccc ttccgcgtag gcttggagct cttgggcagc ccatcgccca cgctgaccgt
     1681 gagggtccga ccgccaacct tgagcactgc caagtcgccg aagccgcggg agaaggtgac
     1741 gctgcgggtg ccgccaccgc cccagccctc cttcttcacc cgaaactgta gtgtgtcgct
     1801 gaaggtgagg ggcaggggcc tccgcgtcgc ctcctcgaag cgcttgcaca gaaggctgac
     1861 aaactcggtc ttgaagacgc tctccaggaa gctgtcggcg gcatcctctt ggaggatgaa
     1921 gaagtcgtcc tgtcgcgtgc tgagggagac tccccgcaga gcctggatgt ccactttctt
     1981 cttcaagact tcacacacct ggcccttctc aggtcccttc ttcactttct ctcgcccaat
     2041 cacatacaca cacttgggcg tcaggatcaa gtcccgcttg atgggcttga agcggcggtc
     2101 gtacttggtg accgaatcgg cgaagtccac ccgctccctc ttgcccagga actgacgcag
     2161 ctcgggccgc tcctccagcc ccaggtagtc cccgacgaag ttccgattga tgctgttgcg
     2221 cctccgctcc ttcttgttca gcaggatgtt ggaagcttcc tcccgcatct cctcgtactt
     2281 ccggacagcc acgtggcgcc gccaggcctt ctggatggtt cgggcaaagc catcgaactt
     2341 tcgctctcgc acctcctcca ggaggaaaag cgactctggg ttcttgacaa agaccttggt
     2401 gctccccatc tggtactggt cgggctccat gttgaccgcc cgaagcaggt gctggacgcc
     2461 ctggcgttcg tccccacgcc accgcggcca cgtctcgggg gtcagaatgg catacctctg
     2521 caggaatttg gcgaactggc ggcggtaggc gaagccggct ctgcgcaccc tgatgttctc
     2581 cttcaggccc aggtattcca cctggtgctt gactctgttc tcctcccagt ctcggggcct
     2641 cttggtctcg ttgggtttga tgcagcggat gtagtggggt gtgcacctca tcagtgtggc
     2701 caccaggtcg ttggcttgtt tcttgatctt ggagccggcg gtgctggggc gccccttctt
     2761 gtctccatcc agcttctcgg ggaagagcat ccggaggaag gcctgctcac tggtctgcat
     2821 cagctctatg aggtcggaga agagaacgtc tcggttcctc tcgcagaagc cgctgacgtc
     2881 gtaggagacc ttgccagcgt agtggtggat gacgaagccg gcgctccagc tgttgaaatg
     2941 ctcgtgggtc cccacagccg cctgcagctt ctgcagcagt gtctggtctg ctcccccgcc
     3001 cgtggcgtgc atggtggcgc acacgtcgtc caagacgctc atgatgcctg gggggctcag
     3061 cttgttttcg atgaggtcac agacgacctt gttgttgaag tactggattg gagtccagcg
     3121 gatgccttcc tgcacatact cctcctgctc ggccttcagg gtaagttcga taaagatttg
     3181 ctgcagcttc tcattgacga agttgatgca aaactgctcg aagccatttt tctggaagat
     3241 ctcgaagccg taaatgtcca gcacaccgat gctgtactct tcctggggtt tctgcatagc
     3301 acggttgatg gcctccacga ggaagtcgaa gaggcgggca tagagcccct tggccagggc
     3361 atcacgggtg taggctgcct gctccacgtt gagggtcaca ttgatggact cgctgcgccc
     3421 gccccagcgg ctgtccatct tgcggctggt cagcttctcc tgcagtcgcc cgctgtcaat
     3481 gcccagcagg taggcgggaa aggccaggag gtccacactc tccactcggg cgtaattccc
     3541 gtcttcacag aaactgatgt tccccaggtg caagatcccc gccacgagct gcaggaccag
     3601 ctgctggatg ctgggcggga tcccaataac ctgcatagca ctcagagtct caccaaagtc
     3661 gcttctgtcg tccgtgccgt ccacctggta ggtgtccgat tggttgaggt agtaatagta
     3721 gtccggtgtc atgaggccca ggttctgcct ttgctcctgg gaggcccctt ccagcagctg
     3781 gtagtagatg tggaagttcc tctcattttc attttgcatg accacgcggg acttctccag
     3841 caagaagttg gagatcttgc ccccatctgg ctccccacct cggctgaact ggatctcaaa
     3901 gtacttgcca aagcggctgg aattgttgtt gcgcacagtc ttggcgttgc cgaaggcctc
     3961 gagcagcggg ttggactgca ggatgatatc tttgacgtgc tggaccttct cgcctccgcc
     4021 agacaccttg gagatgtagc ccatgatata tttggctgcc actgtcttcc cagctccact
     4081 ctctccacta atgatgacac actggttctc acagtcgata agcatgttcc ggtacatgtt
     4141 gtccgtgagg gcgtagatgt gcgggggatt ctcatactgg gccgcgccct gatagaggtc
     4201 gatctcacgg tcggtgaagt agggcatctg cttgaagggg tttacagaga tgagcacaga
     4261 gccgatgtag gtgaagatgt agtcgtccat gaagcgcttc cggaggttgg cggcaatggc
     4321 gtcttcggtg atctggggaa gaagcaccat gtcatccacg ccgctctgct tcacgttgtg
     4381 gctctgccag tggaagcgct ccttgctgcc catagatctg agtccggact tgtacagctc
     4441 gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg
     4501 cttctcgttg gggtctttgc tcagcttgga ctgggtgctc aggtagtggt tgtcgggcag
     4561 cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct
     4621 gccgtcctcg atgttgtggc gggtcttgaa gttcaccttg atgccgttct tctgcttgtc
     4681 ggcggtgata tagaccttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt
     4741 gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc
     4801 gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc
     4861 ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg
     4921 gtagcgggcg aagcactgca cgccgtaggt caaggtggtc acgagggtgg gccagggcac
     4981 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
     5041 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
     5101 gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg cgaccggtag
     5161 cgctagcgga tctgacggtt cactaaacca gctctgctta tatagacctc ccaccgtaca
     5221 cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt
     5281 tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc
     5341 cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta
     5401 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac
     5461 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc
     5521 atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga
     5581 cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat
     5641 gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg
     5701 aactccatat atgggctatg aactaatgac cccgtaattg attactatta ataactaatg
     5761 catggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     5821 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     5881 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     5941 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6001 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6061 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6121 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6181 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6241 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6301 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6361 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     6421 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     6481 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6541 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6601 aagtatatat gagtaacctg aggctatggc agggcctgcc gccccgacgt tggctgcgag
     6661 ccctgggcct tcacccgaac ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta
     6721 ttggccccaa tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc
     6781 gcgtttatga acaaacgacc caacaccgtg cgttttattc tgtcttttta ttgccgtcat
     6841 agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg
     6901 ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa
     6961 aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg
     7021 caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg
     7081 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
     7141 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
     7201 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
     7261 ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg
     7321 ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
     7381 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     7441 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     7501 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     7561 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     7621 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     7681 tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     7741 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     7801 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     7861 atgcgaaacg atcctcatcc tgtctcttga tcgatctttg caaaagccta ggcctccaaa
     7921 aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
     7981 aat
//
