LOCUS       577_pCEFL_GPR161_HA.txt.xdna    7672 bp      DNA     circular   11/6/2025
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : pCEFL WLS HA.txt.xdna [6029  nt] : (NotI[3215]
             / BamHI[1541]) to GPR161 HA gene fragment.xdna [1635  nt] :
             (BamHI[10] / NotI[1648])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     promoter        218..1484
                     /label=EF-1a
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="29" - label=EF-1a - 218..1484 \ 
     CDS             4377..5171
                     /label=Neomycin\(G418\resistance)
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="4" - label=Neomycin(G418resistance) - 2766..3560 \ 
     polyA_signal    3268..3492
                     /label=BGH\pA\signal
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="25" - label=BGHpAsignal - 1657..1881 \ 
     primer_bind     complement(5511..5531)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(3225..3242)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5899..6527)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(5537..5559)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(6679..7338)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4380..5171
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(7578..7606)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5564..5593)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    4161..4238
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3265..3492
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1507..1519
                     /label=SfiI A
                     /SerialCloner_Color=&h80FF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3196..3208
                     /label=SfiI B
                     /SerialCloner_Color=&h80FF80
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             3149..3175
                     /label=HA tag
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1556..3142
                     /label=GPR161
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatcgatc tcccgatccc ctatggtcga ctctcagtac aatctgctct 
       61 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag 
      121 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat 
      181 ctgcttaggg ttaggcgttt tgcgctgctt cgagcttgca aagatggata aagttttaaa 
      241 cagagaggaa tctttgcagc taatggacct tgtaggtctt gaaaggagtg ggaattggct 
      301 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 
      361 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 
      421 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 
      481 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 
      541 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 
      601 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 
      661 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 
      721 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 
      781 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 
      841 caagatagtc ttgtaaatgc gggccaagat cgatctgcac actggtattt cggtttttgg 
      901 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct 
      961 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt 
     1021 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc 
     1081 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg 
     1141 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt 
     1201 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct 
     1261 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc 
     1321 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt gggcacttga 
     1381 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc 
     1441 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgagaattg gcgaagctca 
     1501 gttaacggcc attgtggcca tcaagcttgg taccagatct ggatcCGCCG CCACCATGAG 
     1561 CCTGAACAGC AGCTTGAGTT GTCGCAAAGA GCTTAGTAAT CTGACTGAAG AAGAGGGAGG 
     1621 TGAAGGGGGA GTAATCATTA CACAGTTCAT AGCTATTATA GTCATAACCA TTTTCGTTTG 
     1681 CCTCGGGAAT CTCGTCATAG TAGTTACACT GTACAAAAAA TCTTACTTGC TTACCCTGTC 
     1741 CAACAAGTTT GTATTTTCCC TGACCCTCTC TAACTTCCTT CTTTCTGTGC TTGTACTCCC 
     1801 CTTCGTAGTA ACGTCTTCAA TAAGGAGGGA GTGGATTTTC GGAGTAGTAT GGTGCAATTT 
     1861 TTCAGCGCTG CTGTATCTGC TTATAAGTAG CGCAAGTATG CTTACGCTCG GGGTTATCGC 
     1921 CATTGATCGA TATTACGCGG TATTGTACCC AATGGTATAC CCTATGAAAA TAACGGGAAA 
     1981 CCGAGCCGTC ATGGCACTCG TTTACATTTG GCTCCACTCA CTCATTGGCT GTCTCCCGCC 
     2041 GCTGTTCGGG TGGAGCAGTG TCGAGTTCGA CGAATTTAAG TGGATGTGCG TCGCGGCTTG 
     2101 GCACCGGGAG CCAGGCTATA CGGCCTTTTG GCAAATTTGG TGCGCTTTGT TCCCGTTTTT 
     2161 GGTAATGCTG GTGTGTTATG GCTTTATCTT CCGCGTGGCG CGAGTCAAAG CCCGAAAGGT 
     2221 TCATTGCGGA ACGGTTGTCA TTGTAGAGGA AGATGCTCAA CGCACGGGCC GGAAAAACTC 
     2281 TTCTACCAGC ACGTCATCTT CTGGCAGTCG ACGGAATGCA TTCCAAGGGG TAGTGTACTC 
     2341 CGCCAACCAA TGCAAGGCTC TGATCACGAT TCTGGTGGTA CTTGGCGCGT TCATGGTGAC 
     2401 CTGGGGGCCG TATATGGTCG TTATTGCGTC CGAGGCTCTC TGGGGAAAGT CTTCAGTATC 
     2461 TCCCAGCCTG GAAACTTGGG CAACGTGGCT TTCTTTCGCG TCCGCTGTCT GCCATCCATT 
     2521 GATTTATGGA TTGTGGAATA AAACCGTCCG AAAGGAATTG CTTGGTATGT GCTTCGGTGA 
     2581 TCGCTATTAC AGAGAACCCT TTGTGCAGCG ACAGCGGACC AGCCGATTGT TTTCTATAAG 
     2641 CAATCGGATT ACAGACCTTG GGCTGTCCCC ACACCTTACC GCGCTTATGG CTGGAGGGCA 
     2701 ACCCTTGGGG CATTCAAGTT CTACTGGTGA CACGGGCTTT TCATGTTCCC AGGATTCAGG 
     2761 TACTGATATG ATGCTTCTGG AAGACTACAC GTCCGACGAT AATCCCCCCA GTCATTGTAC 
     2821 ATGTCCGCCC AAACGCCGAT CCTCCGTGAC TTTTGAGGAT GAAGTGGAGC AAATAAAAGA 
     2881 AGCTGCTAAG AACAGCATTC TGCATGTTAA AGCTGAGGTC CACAAGTCAC TCGATAGCTA 
     2941 TGCGGCTAGC CTCGCCAAGG CGATCGAAGC AGAGGCCAAG ATCAACCTGT TCGGCGAAGA 
     3001 GGCGCTCCCA GGCGTCCTGG TAACAGCTCG GACCGTTCCA GGTGGGGGCT TCGGTGGTAG 
     3061 ACGCGGCAGC AGAACTCTGG TCTCCCAGAG GCTGCAGCTG CAAAGCATTG AAGAAGGCGA 
     3121 TGTGCTCGCA GCCGAACAAC GGGAATTCta cccatacgat gttcctgact atgcgTAGGC 
     3181 ggccgcactc gagatggccc tagcggcctc tagagggccc tattctatag tgtcacctaa 
     3241 atgctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt 
     3301 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat 
     3361 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg 
     3421 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg 
     3481 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg 
     3541 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 
     3601 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 
     3661 tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc cgatttagtg 
     3721 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 
     3781 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 
     3841 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 
     3901 ggattttggg gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 
     3961 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagg 
     4021 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 
     4081 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 
     4141 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     4201 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 
     4261 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 
     4321 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga 
     4381 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 
     4441 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 
     4501 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg 
     4561 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 
     4621 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 
     4681 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 
     4741 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 
     4801 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 
     4861 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg 
     4921 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 
     4981 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 
     5041 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 
     5101 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 
     5161 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc 
     5221 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 
     5281 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 
     5341 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 
     5401 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 
     5461 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata 
     5521 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 
     5581 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 
     5641 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 
     5701 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 
     5761 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 
     5821 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 
     5881 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 
     5941 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
     6001 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
     6061 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg 
     6121 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
     6181 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
     6241 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
     6301 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 
     6361 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 
     6421 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 
     6481 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 
     6541 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 
     6601 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 
     6661 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 
     6721 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 
     6781 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 
     6841 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 
     6901 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 
     6961 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 
     7021 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 
     7081 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 
     7141 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 
     7201 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 
     7261 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 
     7321 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 
     7381 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 
     7441 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 
     7501 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 
     7561 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 
     7621 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//