LOCUS       633_pLV-mCherryT2ABsd-EF1A-HA3xGShGLI1.xdna    12149 bp      DNA     circular   11/6/2025
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]:  - [LOCUS]: VB230426-1412cvy - [ACCESSION]: VB
            230426-1412cvy - [SOURCE]: 1..12149 - [CDS]: 3168..6152
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     source          1..12149
                     /label=source
                     /SerialCloner_Color=&h2F4F4F
                     /SerialCloner_Show=False
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc= \ 
                     /SerialCloner_Desc=1..12149 \ 
     Promoter        1..229
                     /label=RSV  promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=application_notes="Strong promoter; drives transcription - of viral RNA in packaging cells." - description="Rous sarcoma virus enhancer/promoter" - full_name="Rous sarcoma virus (RSV) enhancer/promoter" - label="RSV  promoter" - note="color: #7a00b5; dire \ 
     LTR             230..410
                     /label=5' LTR-ΔU3
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=application_notes="Allows transcription of viral RNA and - its packaging into virus." - description="Truncated HIV-1 5' long terminal repeat" - full_name="HIV-1 truncated 5' LTR" - label="5' LTR-ΔU3" - note="color: #05696d; direction: RIGHT" - official_d \ 
     misc_feature    521..565
                     /label=Ψ
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=application_notes="Allows packaging of viral RNA into - virus." - description="HIV-1 packaging signal" - full_name="HIV-1 psi packaging signal" - label="Ψ" - note="color: #f54c6b; direction: RIGHT" - official_designation="Psi" - uuid="284899413ff943a99f8 \ 
     misc_feature    1075..1308
                     /label=RRE
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=application_notes="Rev protein binding site that allows - Rev-dependent nuclear export of viral RNA during viral - packaging." - description="HIV-1 Rev response element" - full_name="HIV-1 Rev response element" - label="RRE" - note="color: #944603; direct \ 
     misc_feature    1803..1920
                     /label=cPPT
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=application_notes="Facilitates the nuclear import of HIV-1 - cDNA through a central DNA flap." - description="Central polypurine tract" - full_name="Central polypurine tract" - label="cPPT" - note="color: #ce0084; direction: RIGHT" - official_designation= \ 
     Promoter        1959..3137
                     /label=EF1A
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=application_notes="Strong promoter." - description="Human eukaryotic translation elongation - factor 1 alpha1 promoter" - full_name="Human eukaryotic translation elongation factor - 1 alpha1 promoter" - label="EF1A" - note="color: #6d3ed8; direction: RIGH \ 
     misc_feature    3162..3167
                     /label=Kozak
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=application_notes="Facilitates translation initiation of - ATG start codon downstream of the Kozak sequence." - description="Kozak translation initiation sequence" - full_name="Kozak" - label="Kozak" - note="color: #0375bb; direction: RIGHT" - official_de \ 
     CDS             3168..6152
                     /label=HA/3xGS/hGLI1[NM_001160045.2]
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=application_notes="" - label="HA/3xGS/hGLI1[NM_001160045.2]" - note="color: #0ed8aa; direction: RIGHT" - official_designation="hGLI1[NM_001160045.2]" - uuid="fb1c1c475c474bb0b0ff1e9d397050b6" -  \ 
                     /SerialCloner_Desc=3168..6152 \ 
     misc_feature    6191..6788
                     /label=WPRE
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=application_notes="Enhances virus stability in packaging - cells, leading to higher titer of packaged virus; enhances - higher expression of transgenes." - description="Woodchuck hepatitis virus posttranscriptional - regulatory element" - full_name="Woodc \ 
     Promoter        6810..7397
                     /label=CMV promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=application_notes="Strong promoter; may have variable - strength in some cell types." - description="Human cytomegalovirus immediate early - promoter" - full_name="Human cytomegalovirus immediate early promoter" - label="CMV promoter" - note="color: #946a \ 
     misc_feature    7429..8598
                     /label=mCherry:T2A:Bsd
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=application_notes="Allows cells to be visualized by red - fluorescence and resistant to blasticidin." - description="mCherry and Bsd linked by T2A" - full_name="mCherry and blasticidin dual reporter gene" - label="mCherry:T2A:Bsd" - note="color: #512bbd;  \ 
     LTR             8669..8903
                     /label=3' LTR-ΔU3
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=application_notes="Allows packaging of viral RNA into - virus; self-inactivates the 5' LTR by a copying mechanism - during viral genome integration; contains polyadenylation - signal for transcription termination." - description="Truncated HIV-1 3' long t \ 
     PolyA_signal    8976..9110
                     /label=SV40 early pA
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=application_notes="Allows transcription termination and - polyadenylation of mRNA transcribed by Pol II RNA - polymerase." - description="Simian virus 40 early polyadenylation signal" - full_name="SV40 early polyadenation signal" - label="SV40 early pA" - \ 
     misc_feature    10064..10924
                     /label=AmpiciIIin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=application_notes="Allows E. coli to be resistant to - ampiciIIin." - description="AmpiciIIin resistance gene" - full_name="Ampicillin resistance gene" - label="AmpiciIIin" - note="color: #db6901; direction: RIGHT" - official_designation="Ampicillin" - uu \ 
     Rep_origin      11095..11683
                     /label=pUC ori
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=application_notes="Facilitates plasmid replication in E. - coli; regulates high-copy plasmid number (500-700)." - description="pUC origin of replication" - full_name="pUC origin of replication" - label="pUC ori" - note="color: #800c0c; direction: RIGHT" - \ 
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca 
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga 
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt 
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg 
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg 
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt 
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga 
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct 
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg 
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa 
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta 
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata 
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt 
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg 
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg 
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc 
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt 
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg 
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg 
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt 
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg 
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt 
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg 
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg 
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac 
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg 
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc 
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg 
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg 
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta 
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg 
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt 
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc 
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg tacccatacg 
     3181 atgttccaga ttacgctggc agcggcagcg gcagcatgag cccatctctg ggattcccag 
     3241 cccagatgaa tcaccaaaaa gggccctcgc cttcctttgg ggtccagcct tgtggtcccc 
     3301 atgactctgc ccggggtggg atgatcccac atcctcagtc ccggggaccc ttcccaactt 
     3361 gccagctgaa gtctgagctg gacatgctgg ttggcaagtg ccgggaggaa cccttggaag 
     3421 gtgatatgtc cagccccaac tccacaggca tacaggatcc cctgttgggg atgctggatg 
     3481 ggcgggagga cctcgagaga gaggagaagc gtgagcctga atctgtgtat gaaactgact 
     3541 gccgttggga tggctgcagc caggaatttg actcccaaga gcagctggtg caccacatca 
     3601 acagcgagca catccacggg gagcggaagg agttcgtgtg ccactggggg ggctgctcca 
     3661 gggagctgag gcccttcaaa gcccagtaca tgctggtggt tcacatgcgc agacacactg 
     3721 gcgagaagcc acacaagtgc acgtttgaag ggtgccggaa gtcatactca cgcctcgaaa 
     3781 acctgaagac gcacctgcgg tcacacacgg gtgagaagcc atacatgtgt gagcacgagg 
     3841 gctgcagtaa agccttcagc aatgccagtg accgagccaa gcaccagaat cggacccatt 
     3901 ccaatgagaa gccgtatgta tgtaagctcc ctggctgcac caaacgctat acagatccta 
     3961 gctcgctgcg aaaacatgtc aagacagtgc atggtcctga cgcccatgtg accaaacggc 
     4021 accgtgggga tggccccctg cctcgggcac catccatttc tacagtggag cccaagaggg 
     4081 agcgggaagg aggtcccatc agggaggaaa gcagactgac tgtgccagag ggtgccatga 
     4141 agccacagcc aagccctggg gcccagtcat cctgcagcag tgaccactcc ccggcaggga 
     4201 gtgcagccaa tacagacagt ggtgtggaaa tgactggcaa tgcagggggc agcactgaag 
     4261 acctctccag cttggacgag ggaccttgca ttgctggcac tggtctgtcc actcttcgcc 
     4321 gccttgagaa cctcaggctg gaccagctac atcaactccg gccaataggg acccggggtc 
     4381 tcaaactgcc cagcttgtcc cacaccggta ccactgtgtc ccgccgcgtg ggccccccag 
     4441 tctctcttga acgccgcagc agcagctcca gcagcatcag ctctgcctat actgtcagcc 
     4501 gccgctcctc cctggcctct cctttccccc ctggctcccc accagagaat ggagcatcct 
     4561 ccctgcctgg ccttatgcct gcccagcact acctgcttcg ggcaagatat gcttcagcca 
     4621 gagggggtgg tacttcgccc actgcagcat ccagcctgga tcggataggt ggtcttccca 
     4681 tgcctccttg gagaagccga gccgagtatc caggatacaa ccccaatgca ggggtcaccc 
     4741 ggagggccag tgacccagcc caggctgctg accgtcctgc tccagctaga gtccagaggt 
     4801 tcaagagcct gggctgtgtc cataccccac ccactgtggc agggggagga cagaactttg 
     4861 atccttacct cccaacctct gtctactcac cacagccccc cagcatcact gagaatgctg 
     4921 ccatggatgc tagagggcta caggaagagc cagaagttgg gacctccatg gtgggcagtg 
     4981 gtctgaaccc ctatatggac ttcccaccta ctgatactct gggatatggg ggacctgaag 
     5041 gggcagcagc tgagccttat ggagcgaggg gtccaggctc tctgcctctt gggcctggtc 
     5101 cacccaccaa ctatggcccc aacccctgtc cccagcaggc ctcatatcct gaccccaccc 
     5161 aagaaacatg gggtgagttc ccttcccact ctgggctgta cccaggcccc aaggctctag 
     5221 gtggaaccta cagccagtgt cctcgacttg aacattatgg acaagtgcaa gtcaagccag 
     5281 aacaggggtg cccagtgggg tctgactcca caggactggc accctgcctc aatgcccacc 
     5341 ccagtgaggg gcccccacat ccacagcctc tcttttccca ttacccccag ccctctcctc 
     5401 cccaatatct ccagtcaggc ccctataccc agccaccccc tgattatctt ccttcagaac 
     5461 ccaggccttg cctggacttt gattccccca cccattccac agggcagctc aaggctcagc 
     5521 ttgtgtgtaa ttatgttcaa tctcaacagg agctactgtg ggagggtggg ggcagggaag 
     5581 atgcccccgc ccaggaacct tcctaccaga gtcccaagtt tctggggggt tcccaggtta 
     5641 gcccaagccg tgctaaagct ccagtgaaca catatggacc tggctttgga cccaacttgc 
     5701 ccaatcacaa gtcaggttcc tatcccaccc cttcaccatg ccatgaaaat tttgtagtgg 
     5761 gggcaaatag ggcttcacat agggcagcag caccacctcg acttctgccc ccattgccca 
     5821 cttgctatgg gcctctcaaa gtgggaggca caaaccccag ctgtggtcat cctgaggtgg 
     5881 gcaggctagg agggggtcct gccttgtacc ctcctcccga aggacaggta tgtaaccccc 
     5941 tggactctct tgatcttgac aacactcagc tggactttgt ggctattctg gatgagcccc 
     6001 aggggctgag tcctcctcct tcccatgatc agcggggcag ctctggacat accccacctc 
     6061 cctctgggcc ccccaacatg gctgtgggca acatgagtgt cttactgaga tccctacctg 
     6121 gggaaacaga attcctcaac tctagtgcct aaacccagct ttcttgtaca aagtggtgat 
     6181 aatcgaattc cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 
     6241 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 
     6301 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 
     6361 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 
     6421 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 
     6481 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 
     6541 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 
     6601 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 
     6661 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 
     6721 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 
     6781 cgcatcggga attcccgcgg ttcgaacgcg ttgacattga ttattgacta gttattaata 
     6841 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 
     6901 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 
     6961 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 
     7021 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 
     7081 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 
     7141 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg 
     7201 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct 
     7261 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa 
     7321 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt 
     7381 ctatataagc agagctctct ggctaactag agaacccact gcgccaccat ggtgagcaag 
     7441 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag 
     7501 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag 
     7561 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac 
     7621 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc 
     7681 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc 
     7741 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc 
     7801 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag 
     7861 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc 
     7921 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc 
     7981 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg 
     8041 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc 
     8101 cgccactcca ccggcggcat ggacgagctg tacaagggct ccggagaggg caggggaagt 
     8161 cttctaacat gcggggacgt ggaggaaaat cccggcccca tggccaagcc tttgtctcaa 
     8221 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa 
     8281 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat 
     8341 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct 
     8401 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg 
     8461 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata 
     8521 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt 
     8581 tatgtgtggg agggctaagg tacctttaag accaatgact tacaaggcag ctgtagatct 
     8641 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca 
     8701 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 
     8761 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 
     8821 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 
     8881 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 
     8941 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 
     9001 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 
     9061 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg 
     9121 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 
     9181 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 
     9241 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg 
     9301 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 
     9361 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 
     9421 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg 
     9481 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 
     9541 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 
     9601 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 
     9661 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 
     9721 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 
     9781 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 
     9841 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 
     9901 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg 
     9961 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 
    10021 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 
    10081 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 
    10141 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 
    10201 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 
    10261 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 
    10321 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 
    10381 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 
    10441 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 
    10501 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 
    10561 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 
    10621 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 
    10681 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 
    10741 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 
    10801 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 
    10861 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 
    10921 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 
    10981 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 
    11041 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 
    11101 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 
    11161 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 
    11221 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 
    11281 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 
    11341 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 
    11401 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 
    11461 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagagagaaa 
    11521 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 
    11581 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 
    11641 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 
    11701 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 
    11761 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 
    11821 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa 
    11881 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga 
    11941 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc 
    12001 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca 
    12061 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta 
    12121 aagggaacaa aagctggagc tgcaagctt
//