LOCUS       650_2A_pCEFL_SfiI__PRKACA_HA_WT.xdna    7137 bp      DNA     circular   11/6/2025
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: . - [LOCUS]: 01_RIB_01_2A_PKACA_WT - [ACCESSIO
            N]: urn.local...12-ig17g7h
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     primer_bind     complement(5363..5383)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(3078..3095)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5751..6379)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(5389..5411)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(6531..53)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4232..5023
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(293..321)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5416..5445)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    4013..4090
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3118..3345
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2999..3025
                     /label=HA tag
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1940..2992
                     /label=PKA WT
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 
       61 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 
      121 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 
      181 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 
      241 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 
      301 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 
      361 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta 
      421 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct 
      481 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa 
      541 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcga 
      601 gctttgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta atggaccttc 
      661 taggtcttga aaggagtggg aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg 
      721 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg 
      781 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 
      841 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 
      901 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt 
      961 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc 
     1021 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg 
     1081 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg 
     1141 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga 
     1201 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatcgatct 
     1261 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg 
     1321 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc 
     1381 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg 
     1441 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg 
     1501 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc 
     1561 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga 
     1621 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt 
     1681 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga 
     1741 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg 
     1801 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt 
     1861 gtcgtgagga attggcgaag ctcagttaac ggccattgtg gccatcaagc ttggtaccag 
     1921 atctggatcc gccgccacca tgggcaacgc cgccgccgcc aagaagggca gcgagcagga 
     1981 gagcgtgaaa gaattcttag ccaaagccaa agaagatttt cttaaaaaat gggaaagtcc 
     2041 cgctcagaac acagcccact tggatcagtt tgaacgaatc aagaccctcg gcacgggctc 
     2101 cttcgggcgg gtgatgctgg tgaaacacaa ggagaccggg aaccactatg ccatgaagat 
     2161 cctcgacaaa cagaaggtgg tgaaactgaa acagatcgaa cacaccctga atgaaaagcg 
     2221 catcctgcaa gctgtcaact ttccgttcct cgtcaaactc gagttctcct tcaaggacaa 
     2281 ctcaaactta tacatggtca tggagtacgt gcccggcggg gagatgttct cacacctacg 
     2341 gcggatcgga aggttcagtg agccccatgc ccgtttctac gcggcccaga tcgtcctgac 
     2401 ctttgagtat ctgcactcgc tggatctcat ctacagggac ctgaagccgg agaatctgct 
     2461 cattgaccag cagggctaca ttcaggtgac agacttcggt ttcgccaagc gcgtgaaggg 
     2521 ccgcacttgg accttgtgcg gcacccctga gtacctggcc cctgagatta tcctgagcaa 
     2581 aggctacaac aaggccgtgg actggtgggc cctgggggtt cttatctatg aaatggccgc 
     2641 tggctacccg cccttcttcg cagaccagcc catccagatc tatgagaaga tcgtctctgg 
     2701 gaaggtgcgc ttcccttccc acttcagctc tgacttgaag gacctgctgc ggaacctcct 
     2761 gcaggtagat ctcaccaagc gctttgggaa cctcaagaat ggggtcaacg atatcaagaa 
     2821 ccacaagtgg tttgccacaa ctgactggat tgccatctac cagaggaagg tggaagctcc 
     2881 cttcatacca aagtttaaag gccctgggga tacgagtaac tttgacgact atgaggaaga 
     2941 agaaatccgg gtctccatca atgagaagtg tggcaaggag ttttctgagt ttgctagcta 
     3001 cccatacgac gtgccagatt acgcctaggc ggccgcactc gagatatcgg cctccagggc 
     3061 ctctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga 
     3121 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc 
     3181 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc 
     3241 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt 
     3301 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa 
     3361 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg 
     3421 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc 
     3481 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa 
     3541 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac 
     3601 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt 
     3661 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca 
     3721 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt 
     3781 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca 
     3841 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 
     3901 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 
     3961 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 
     4021 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 
     4081 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt 
     4141 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga 
     4201 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 
     4261 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 
     4321 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 
     4381 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc 
     4441 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 
     4501 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 
     4561 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 
     4621 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 
     4681 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 
     4741 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 
     4801 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 
     4861 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 
     4921 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 
     4981 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 
     5041 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc 
     5101 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag 
     5161 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat 
     5221 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 
     5281 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc 
     5341 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 
     5401 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 
     5461 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 
     5521 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 
     5581 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 
     5641 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 
     5701 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 
     5761 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 
     5821 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 
     5881 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 
     5941 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 
     6001 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 
     6061 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 
     6121 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 
     6181 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 
     6241 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 
     6301 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 
     6361 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     6421 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 
     6481 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 
     6541 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 
     6601 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 
     6661 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 
     6721 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 
     6781 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 
     6841 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 
     6901 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 
     6961 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 
     7021 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 
     7081 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccg
//