LOCUS       652_3A_pCEFL_SfiI__PRKACA_HA_T49I.xdna    7137 bp      DNA     circular   11/6/2025
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: . - [LOCUS]: 04_RIB_04_3A_PKA_T49I - [ACCESSIO
            N]: urn.local...15-ig17g7j
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     primer_bind     complement(5360..5380)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(3075..3092)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5748..6376)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(5386..5408)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(6528..50)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4229..5020
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(290..318)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5413..5442)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    4010..4087
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3115..3342
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2996..3022
                     /label=HA tag
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1937..2989
                     /label=PKA T49I
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 
       61 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 
      121 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 
      181 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 
      241 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 
      301 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
      361 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc gatctcccga tcccctatgg 
      421 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct 
      481 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc 
      541 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgagct 
      601 ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag 
      661 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc 
      721 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg 
      781 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg 
      841 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc 
      901 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat 
      961 ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag 
     1021 cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct 
     1081 cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac 
     1141 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct 
     1201 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tcgatctgca 
     1261 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac 
     1321 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca 
     1381 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc 
     1441 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc 
     1501 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc 
     1561 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta 
     1621 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg 
     1681 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt 
     1741 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc 
     1801 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc 
     1861 gtgaggaatt ggcgaagctc agttaacggc cattgtggcc atcaagcttg gtaccagatc 
     1921 tggatccgcc gccaccatgg gcaacgccgc cgccgccaag aagggcagcg agcaggagag 
     1981 cgtgaaagaa ttcttagcca aagccaaaga agattttctt aaaaaatggg aaagtcccgc 
     2041 tcagaacaca gcccacttgg atcagtttga acgaatcaag atcctcggca cgggctcctt 
     2101 cgggcgggtg atgctggtga aacacaagga gaccgggaac cactatgcca tgaagatcct 
     2161 cgacaaacag aaggtggtga aactgaaaca gatcgaacac accctgaatg aaaagcgcat 
     2221 cctgcaagct gtcaactttc cgttcctcgt caaactcgag ttctccttca aggacaactc 
     2281 aaacttatac atggtcatgg agtacgtgcc cggcggggag atgttctcac acctacggcg 
     2341 gatcggaagg ttcagtgagc cccatgcccg tttctacgcg gcccagatcg tcctgacctt 
     2401 tgagtatctg cactcgctgg atctcatcta cagggacctg aagccggaga atctgctcat 
     2461 tgaccagcag ggctacattc aggtgacaga cttcggtttc gccaagcgcg tgaagggccg 
     2521 cacttggacc ttgtgcggca cccctgagta cctggcccct gagattatcc tgagcaaagg 
     2581 ctacaacaag gccgtggact ggtgggccct gggggttctt atctatgaaa tggccgctgg 
     2641 ctacccgccc ttcttcgcag accagcccat ccagatctat gagaagatcg tctctgggaa 
     2701 ggtgcgcttc ccttcccact tcagctctga cttgaaggac ctgctgcgga acctcctgca 
     2761 ggtagatctc accaagcgct ttgggaacct caagaatggg gtcaacgata tcaagaacca 
     2821 caagtggttt gccacaactg actggattgc catctaccag aggaaggtgg aagctccctt 
     2881 cataccaaag tttaaaggcc ctggggatac gagtaacttt gacgactatg aggaagaaga 
     2941 aatccgggtc tccatcaatg agaagtgtgg caaggagttt tctgagtttg ctagctaccc 
     3001 atacgacgtg ccagattacg cctaggcggc cgcactcgag atatcggcct ccagggcctc 
     3061 tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg 
     3121 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg 
     3181 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga 
     3241 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg 
     3301 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa 
     3361 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg 
     3421 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 
     3481 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 
     3541 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 
     3601 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 
     3661 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 
     3721 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 
     3781 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt 
     3841 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa 
     3901 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 
     3961 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct 
     4021 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 
     4081 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg 
     4141 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca 
     4201 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc 
     4261 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc 
     4321 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga 
     4381 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac 
     4441 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct 
     4501 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa 
     4561 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc 
     4621 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct 
     4681 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc 
     4741 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg 
     4801 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct 
     4861 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct 
     4921 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca 
     4981 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa 
     5041 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc 
     5101 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 
     5161 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt 
     5221 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 
     5281 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct 
     5341 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 
     5401 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 
     5461 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 
     5521 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 
     5581 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     5641 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 
     5701 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     5761 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     5821 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     5881 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     5941 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     6001 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     6061 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     6121 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     6181 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 
     6241 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     6301 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
     6361 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
     6421 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 
     6481 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 
     6541 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 
     6601 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 
     6661 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 
     6721 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 
     6781 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 
     6841 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 
     6901 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 
     6961 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 
     7021 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 
     7081 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcg
//