LOCUS       653_4B_pCEFL_SfiI__PRKACA_HA_E128K.xdna    7137 bp      DNA     circular   11/6/2025
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: . - [LOCUS]: 06_RIB_06_4B_PKA_E128K - [ACCESSI
            ON]: urn.local...18-ig17g7o
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     primer_bind     1888..1908
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     4176..4193
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      892..1520
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    1860..1882
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             81..740
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(2248..3039)
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        6950..6978
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        1826..1855
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    complement(3181..3258)
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      complement(3926..4153)
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(4246..4272)
                     /label=HA tag
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    4279..5331
                     /label=PKA E128K
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 
       61 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 
      121 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 
      181 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 
      241 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 
      301 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 
      361 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 
      421 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 
      481 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 
      541 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 
      601 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 
      661 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 
      721 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 
      781 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 
      841 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 
      901 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 
      961 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 
     1021 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 
     1081 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 
     1141 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 
     1201 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 
     1261 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 
     1321 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 
     1381 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 
     1441 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 
     1501 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 
     1561 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 
     1621 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 
     1681 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 
     1741 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 
     1801 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 
     1861 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc 
     1921 tctagctaga ggtcgacggt atacagacat gataagatac attgatgagt ttggacaaac 
     1981 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt 
     2041 atttgtaacc attataagct gcaataaaca agttggggtg ggcgaagaac tccagcatga 
     2101 gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc 
     2161 tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt 
     2221 cggtcatttc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc 
     2281 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc 
     2341 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc 
     2401 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt 
     2461 cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt 
     2521 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg 
     2581 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg 
     2641 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat 
     2701 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc 
     2761 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac 
     2821 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc 
     2881 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc 
     2941 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca 
     3001 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc 
     3061 tgtctcttga tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc 
     3121 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc 
     3181 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg 
     3241 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag 
     3301 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc 
     3361 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc 
     3421 acaccctaac tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc 
     3481 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc 
     3541 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 
     3601 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca 
     3661 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg 
     3721 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 
     3781 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 
     3841 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc 
     3901 cagctggttc tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta 
     3961 ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga 
     4021 cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg 
     4081 gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta 
     4141 gaaggcacag tcgaggctga tcagcgagct ctagcattta ggtgacacta tagaataggg 
     4201 ccctctagag gccctggagg ccgatatctc gagtgcggcc gcctaggcgt aatctggcac 
     4261 gtcgtatggg tagctagcaa actcagaaaa ctccttgcca cacttctcat tgatggagac 
     4321 ccggatttct tcttcctcat agtcgtcaaa gttactcgta tccccagggc ctttaaactt 
     4381 tggtatgaag ggagcttcca ccttcctctg gtagatggca atccagtcag ttgtggcaaa 
     4441 ccacttgtgg ttcttgatat cgttgacccc attcttgagg ttcccaaagc gcttggtgag 
     4501 atctacctgc aggaggttcc gcagcaggtc cttcaagtca gagctgaagt gggaagggaa 
     4561 gcgcaccttc ccagagacga tcttctcata gatctggatg ggctggtctg cgaagaaggg 
     4621 cgggtagcca gcggccattt catagataag aacccccagg gcccaccagt ccacggcctt 
     4681 gttgtagcct ttgctcagga taatctcagg ggccaggtac tcaggggtgc cgcacaaggt 
     4741 ccaagtgcgg cccttcacgc gcttggcgaa accgaagtct gtcacctgaa tgtagccctg 
     4801 ctggtcaatg agcagattct ccggcttcag gtccctgtag atgagatcca gcgagtgcag 
     4861 atactcaaag gtcaggacga tctgggccgc gtagaaacgg gcatggggct cactgaacct 
     4921 tccgatccgc cgtaggtgtg agaacatctt cccgccgggc acgtactcca tgaccatgta 
     4981 taagtttgag ttgtccttga aggagaactc gagtttgacg aggaacggaa agttgacagc 
     5041 ttgcaggatg cgcttttcat tcagggtgtg ttcgatctgt ttcagtttca ccaccttctg 
     5101 tttgtcgagg atcttcatgg catagtggtt cccggtctcc ttgtgtttca ccagcatcac 
     5161 ccgcccgaag gagcccgtgc cgagggtctt gattcgttca aactgatcca agtgggctgt 
     5221 gttctgagcg ggactttccc attttttaag aaaatcttct ttggctttgg ctaagaattc 
     5281 tttcacgctc tcctgctcgc tgcccttctt ggcggcggcg gcgttgccca tggtggcggc 
     5341 ggatccagat ctggtaccaa gcttgatggc cacaatggcc gttaactgag cttcgccaat 
     5401 tcctcacgac acctgaaatg gaagaaaaaa actttgaacc actgtctgag gcttgagaat 
     5461 gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag aattacatca agtgccaagc 
     5521 tggcctaact tcagtctcca cccactcagt gtggggaaac tccatcgcat aaaacccctc 
     5581 cccccaacct aaagacgacg tactccaaaa gctcgagaac taatcgaggt gcctggacgg 
     5641 cgcccggtac tccgtggagt cacatgaagc gacggctgag gacggaaagg cccttttcct 
     5701 ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc gtcctccatt ttgagctccc 
     5761 tgcagcaggg ccgggaagcg gccatctttc cgctcacgca actggtgccg accgggccag 
     5821 ccttgccgcc cagggcgggg cgatacacgg cggcgcgagg ccaggcacca gagcaggccg 
     5881 gccagcttga gactaccccc gtccgattct cggtggccgc gctcgcaggc cccgcctcgc 
     5941 cgaacatgtg cgctgggacg cacgggcccc gtcgccgccc gcggccccaa aaaccgaaat 
     6001 accagtgtgc agatcgatct tggcccgcat ttacaagact atcttgccag aaaaaaagcg 
     6061 tcgcagcagg tcatcaaaaa ttttaaatgg ctagagactt atcgaaagca gcgagacagg 
     6121 cgcgaaggtg ccaccagatt cgcacgcggc ggccccagcg cccaggccag gcctcaactc 
     6181 aagcacgagg cgaaggggct ccttaagcgc aaggcctcga actctcccac ccacttccaa 
     6241 cccgaagctc gggatcaaga atcacgtact gcagccaggt ggaagtaatt caaggcacgc 
     6301 aagggccata acccgtaaag aggccaggcc cgcgggaacc acacacggca cttacctgtg 
     6361 ttctggcggc aaacccgttg cgaaaaagaa cgttcacggc gactactgca cttatatacg 
     6421 gttctccccc accctcggga aaaaggcgga gccagtacac gacatcactt tcccagttta 
     6481 ccccgcgcca ccttctctag gcaccggttc aattgccgac ccctcccccc aacttctcgg 
     6541 ggactgtggg cgatgtgcgc tctgcccact gacgggcacc ggagccaatt cccactcctt 
     6601 tcaagaccta gaaggtccat tagctgcaaa gattcctctc tgtttaaaac tttatccatc 
     6661 tttgcaaagc tcgaagcagc gcaaaacgcc taaccctaag cagattcttc atgcaattgt 
     6721 cggtcaagcc ttgccttgtt gtagcttaaa ttttgctcgc gcactactca gcgacctcca 
     6781 acacacaagc agggagcaga tactggctta actatgcggc atcagagcag attgtactga 
     6841 gagtgcacca taggggatcg ggagatcgat ctcccgatcc gtcgacgtca ggtggcactt 
     6901 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 
     6961 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 
     7021 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 
     7081 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtg
//