LOCUS       654_5A_pCEFL_SfiI__PRKACA_HA_R135W.xdna    7137 bp      DNA     circular   11/6/2025
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: . - [LOCUS]: 12_RIB_05 - [ACCESSION]: urn.loca
            l...2d-i40uquf
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     primer_bind     complement(5216..5236)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(2931..2948)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5604..6232)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(5242..5264)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(6384..7043)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4085..4876
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(146..174)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5269..5298)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3866..3943
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      2971..3198
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2852..2878
                     /label=HA tag
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 
       61 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 
      121 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
      181 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 
      241 gacggatcgg gagatcgatc tcccgatccc ctatggtgca ctctcagtac aatctgctct 
      301 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag 
      361 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat 
      421 ctgcttaggg ttaggcgttt tgcgctgctt cgagctttgc aaagatggat aaagttttaa 
      481 acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc 
      541 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 
      601 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 
      661 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 
      721 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg 
      781 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact 
      841 tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag 
      901 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct 
      961 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc 
     1021 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa 
     1081 gatagtcttg taaatgcggg ccaagatcga tctgcacact ggtatttcgg tttttggggc 
     1141 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg 
     1201 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc 
     1261 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc 
     1321 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag 
     1381 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc 
     1441 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga 
     1501 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat 
     1561 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta 
     1621 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac 
     1681 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggaattggcg aagctcagtt 
     1741 aacggccatt gtggccatca agcttggtac cagatctgga tccgccgcca ccatgggcaa 
     1801 cgccgccgcc gccaagaagg gcagcgagca ggagagcgtg aaagaattct tagccaaagc 
     1861 caaagaagat tttcttaaaa aatgggaaag tcccgctcag aacacagccc acttggatca 
     1921 gtttgaacga atcaagaccc tcggcacggg ctccttcggg cgggtgatgc tggtgaaaca 
     1981 caaggagacc gggaaccact atgccatgaa gatcctcgac aaacagaagg tggtgaaact 
     2041 gaaacagatc gaacacaccc tgaatgaaaa gcgcatcctg caagctgtca actttccgtt 
     2101 cctcgtcaaa ctcgagttct ccttcaagga caactcaaac ttatacatgg tcatggagta 
     2161 cgtgcccggc ggggagatgt tctcacacct acggtggatc ggaaggttca gtgagcccca 
     2221 tgcccgtttc tacgcggccc agatcgtcct gacctttgag tatctgcact cgctggatct 
     2281 catctacagg gacctgaagc cggagaatct gctcattgac cagcagggct acattcaggt 
     2341 gacagacttc ggtttcgcca agcgcgtgaa gggccgcact tggaccttgt gcggcacccc 
     2401 tgagtacctg gcccctgaga ttatcctgag caaaggctac aacaaggccg tggactggtg 
     2461 ggccctgggg gttcttatct atgaaatggc cgctggctac ccgcccttct tcgcagacca 
     2521 gcccatccag atctatgaga agatcgtctc tgggaaggtg cgcttccctt cccacttcag 
     2581 ctctgacttg aaggacctgc tgcggaacct cctgcaggta gatctcacca agcgctttgg 
     2641 gaacctcaag aatggggtca acgatatcaa gaaccacaag tggtttgcca caactgactg 
     2701 gattgccatc taccagagga aggtggaagc tcccttcata ccaaagttta aaggccctgg 
     2761 ggatacgagt aactttgacg actatgagga agaagaaatc cgggtctcca tcaatgagaa 
     2821 gtgtggcaag gagttttctg agtttgctag ctacccatac gacgtgccag attacgccta 
     2881 ggcggccgca ctcgagatat cggcctccag ggcctctaga gggccctatt ctatagtgtc 
     2941 acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 
     3001 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 
     3061 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 
     3121 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 
     3181 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc 
     3241 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
     3301 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
     3361 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
     3421 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
     3481 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
     3541 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
     3601 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
     3661 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc 
     3721 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 
     3781 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 
     3841 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 
     3901 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 
     3961 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 
     4021 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg 
     4081 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 
     4141 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 
     4201 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 
     4261 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 
     4321 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 
     4381 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 
     4441 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 
     4501 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 
     4561 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 
     4621 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 
     4681 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 
     4741 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 
     4801 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 
     4861 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 
     4921 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 
     4981 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 
     5041 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 
     5101 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 
     5161 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 
     5221 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 
     5281 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 
     5341 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 
     5401 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 
     5461 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     5521 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     5581 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     5641 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     5701 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     5761 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 
     5821 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     5881 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     5941 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     6001 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
     6061 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     6121 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     6181 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     6241 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 
     6301 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 
     6361 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 
     6421 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 
     6481 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 
     6541 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 
     6601 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 
     6661 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 
     6721 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 
     6781 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 
     6841 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 
     6901 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 
     6961 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 
     7021 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 
     7081 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatct
//