LOCUS       655_6A_pCEFL_SfiI__PRKACA_HA_E171K.xdna    7137 bp      DNA     circular   11/6/2025
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: . - [LOCUS]: cluster_001_consensus - [ACCESSIO
            N]: urn.local...31-i80uhwv
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     primer_bind     complement(4976..4996)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(2691..2708)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5364..5992)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(5002..5024)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(6144..6803)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             3845..4636
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(7043..7071)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5029..5058)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3626..3703
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      2731..2958
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2612..2638
                     /label=HA tag
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1553..2641
                     /label=PKA E171K
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatcgatc tcccgatccc ctatggtgca ctctcagtac aatctgctct 
       61 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag 
      121 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat 
      181 ctgcttaggg ttaggcgttt tgcgctgctt cgagctttgc aaagatggat aaagttttaa 
      241 acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc 
      301 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga 
      361 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat 
      421 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta 
      481 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg 
      541 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact 
      601 tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag 
      661 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct 
      721 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc 
      781 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa 
      841 gatagtcttg taaatgcggg ccaagatcga tctgcacact ggtatttcgg tttttggggc 
      901 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg 
      961 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc 
     1021 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc 
     1081 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag 
     1141 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc 
     1201 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga 
     1261 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat 
     1321 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta 
     1381 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac 
     1441 agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggaattggcg aagctcagtt 
     1501 aacggccatt gtggccatca agcttggtac cagatctgga tccgccgcca ccatgggcaa 
     1561 cgccgccgcc gccaagaagg gcagcgagca ggagagcgtg aaagaattct tagccaaagc 
     1621 caaagaagat tttcttaaaa aatgggaaag tcccgctcag aacacagccc acttggatca 
     1681 gtttgaacga atcaagaccc tcggcacggg ctccttcggg cgggtgatgc tggtgaaaca 
     1741 caaggagacc gggaaccact atgccatgaa gatcctcgac aaacagaagg tggtgaaact 
     1801 gaaacagatc gaacacaccc tgaatgaaaa gcgcatcctg caagctgtca actttccgtt 
     1861 cctcgtcaaa ctcgagttct ccttcaagga caactcaaac ttatacatgg tcatggagta 
     1921 cgtgcccggc ggggagatgt tctcacacct acggcggatc ggaaggttca gtgagcccca 
     1981 tgcccgtttc tacgcggccc agatcgtcct gacctttgag tatctgcact cgctggatct 
     2041 catctacagg gacctgaagc cgaagaatct gctcattgac cagcagggct acattcaggt 
     2101 gacagacttc ggtttcgcca agcgcgtgaa gggccgcact tggaccttgt gcggcacccc 
     2161 tgagtacctg gcccctgaga ttatcctgag caaaggctac aacaaggccg tggactggtg 
     2221 ggccctgggg gttcttatct atgaaatggc cgctggctac ccgcccttct tcgcagacca 
     2281 gcccatccag atctatgaga agatcgtctc tgggaaggtg cgcttccctt cccacttcag 
     2341 ctctgacttg aaggacctgc tgcggaacct cctgcaggta gatctcacca agcgctttgg 
     2401 gaacctcaag aatggggtca acgatatcaa gaaccacaag tggtttgcca caactgactg 
     2461 gattgccatc taccagagga aggtggaagc tcccttcata ccaaagttta aaggccctgg 
     2521 ggatacgagt aactttgacg actatgagga agaagaaatc cgggtctcca tcaatgagaa 
     2581 gtgtggcaag gagttttctg agtttgctag ctacccatac gacgtgccag attacgccta 
     2641 ggcggccgca ctcgagatat cggcctccag ggcctctaga gggccctatt ctatagtgtc 
     2701 acctaaatgc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 
     2761 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 
     2821 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 
     2881 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 
     2941 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc 
     3001 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
     3061 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
     3121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
     3181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
     3241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
     3301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
     3361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
     3421 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc 
     3481 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 
     3541 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 
     3601 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 
     3661 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 
     3721 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 
     3781 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg 
     3841 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 
     3901 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 
     3961 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 
     4021 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 
     4081 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 
     4141 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 
     4201 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 
     4261 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 
     4321 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 
     4381 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 
     4441 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 
     4501 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 
     4561 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 
     4621 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 
     4681 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 
     4741 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 
     4801 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 
     4861 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 
     4921 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 
     4981 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 
     5041 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 
     5101 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 
     5161 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 
     5221 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     5281 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     5341 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     5401 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     5461 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     5521 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 
     5581 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     5641 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     5701 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     5761 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
     5821 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     5881 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     5941 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     6001 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 
     6061 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 
     6121 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 
     6181 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 
     6241 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 
     6301 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 
     6361 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 
     6421 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 
     6481 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 
     6541 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 
     6601 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 
     6661 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 
     6721 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 
     6781 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 
     6841 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 
     6901 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 
     6961 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 
     7021 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 
     7081 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//