LOCUS       658_pCEFL_SfiI__GNAS_EE_K32R.xdna    7242 bp      DNA     circular   1/16/2026
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: sample_1. - [LOCUS]: sample_1 - [ACCESSION]: s
            ample_1 - [CDS]: complement(1338..2129)
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     rep_origin      3497..3925
                     /label=f1 ori
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="f1 ori" - database="snapgene" - identity="100.0" - match_length="100.0" - fragment="False" - other="rep_origin" - description="f1 bacteriophage origin of replication; arrow - indicates direction of (+) strand synthesis " -  \ 
                     /SerialCloner_Desc=com \ 
     promoter        687..1868
                     /label=EF-1Î± promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="EF-1Î± promoter" - database="snapgene" - identity="99.3" - match_length="100.0" - fragment="False" - other="promoter" - description="strong constitutive promoter for human - elongation factor EF-1Î±" -  \ 
                     /SerialCloner_Desc=complement(4596..5777) \ 
     CDS             4335..5126
                     /label=KKA2_KLEPN
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="KKA2_KLEPN" - database="swissprot" - identity="100.0" - match_length="100.0" - fragment="False" - other="CDS" - description="Protein evidence at protein level: Swiss-Prot - protein existence level 1. Resistance to kanamycin, - n \ 
     CDS             complement(6634..252)
                     /label=AmpR
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="AmpR" - database="snapgene" - identity="99.8" - match_length="100.0" - fragment="False" - other="CDS" - description="Î=-lactamase; bla; confers resistance to - ampicillin, carbenicillin, and related antibiotics" -  \ 
                     /SerialCloner_Desc=6212..7072 \ 
     polyA_signal    3227..3451
                     /label=bGH poly(A) signal
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="bGH poly(A) signal" - database="snapgene" - identity="100.0" - match_length="100.0" - fragment="False" - other="polyA_signal" - description="bovine growth hormone polyadenylation signal" -  \ 
                     /SerialCloner_Desc=complement(3013..3237) \ 
     rep_origin      complement(5875..6463)
                     /label=ori
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="ori" - database="snapgene" - identity="99.8" - match_length="100.0" - fragment="False" - other="rep_origin" - description="high-copy-number ColE1/pMB1/pBR322/pUC origin - of replication " -  \ 
                     /SerialCloner_Desc=1..589 \ 
     CDS             1947..3128
                     /label=GNAS EE K32R
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="GNAS2_HUMAN" - database="swissprot" - identity="98.7" - match_length="100.0" - fragment="False" - other="CDS" - description="Protein evidence at protein level: Swiss-Prot - protein existence level 1. Guanine nucleotide-binding - \ 
     polyA_signal    5303..5424
                     /label=SV40 poly(A) signal
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="SV40 poly(A) signal" - database="snapgene" - identity="100.0" - match_length="100.0" - fragment="False" - other="polyA_signal" - description="SV40 polyadenylation signal" -  \ 
                     /SerialCloner_Desc=complement(1040..1161) \ 
     promoter        complement(253..357)
                     /label=AmpR promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="AmpR promoter" - database="snapgene" - identity="100.0" - match_length="100.0" - fragment="False" - other="promoter" - description="bla" -  \ 
                     /SerialCloner_Desc=6107..6211 \ 
     promoter        3939..4268
                     /label=SV40 promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="SV40 promoter" - database="snapgene" - identity="99.7" - match_length="100.0" - fragment="False" - other="promoter" - description="SV40 enhancer and early promoter" -  \ 
                     /SerialCloner_Desc=complement(2196..2525) \ 
     promoter        complement(5521..5551)
                     /label=lac promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="lac promoter" - database="snapgene" - identity="100.0" - match_length="100.0" - fragment="False" - other="promoter" - description="promoter for the E. coli lac operon" -  \ 
                     /SerialCloner_Desc=913..943 \ 
     protein_bind    complement(5566..5587)
                     /label=CAP binding site
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="CAP binding site" - database="snapgene" - identity="100.0" - match_length="100.0" - fragment="False" - other="protein_bind" - description="CAP binding activates transcription in the - presence of cAMP. E. coli catabolite activat \ 
     promoter        complement(3183..3201)
                     /label=SP6 promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="SP6 promoter" - database="snapgene" - identity="100.0" - match_length="100.0" - fragment="False" - other="promoter" - description="promoter for bacteriophage SP6 RNA - polymerase" -  \ 
                     /SerialCloner_Desc=3263..3281 \ 
     protein_bind    complement(5497..5513)
                     /label=lac operator
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="lac operator" - database="snapgene" - identity="100.0" - match_length="100.0" - fragment="False" - other="protein_bind" - description="The lac repressor binds to the lac operator - to inhibit transcription in E. coli. This inhib \ 
     CDS             5150..5308
                     /label=BLE_KLEPN (fragment)
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="BLE_KLEPN (fragment)" - database="swissprot" - identity="98.1" - match_length="42.1" - fragment="True" - other="CDS" - description="Protein evidence at protein level: Swiss-Prot - protein existence level 1. Binding protein with  \ 
     CDS             613..679
                     /label=small t antigen (fragment)
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="small t antigen (fragment)" - database="snapgene" - identity="100.0" - match_length="12.8" - fragment="True" - other="CDS" - description="SV40 (simian virus 40) small t antigen" -  \ 
                     /SerialCloner_Desc=complement(5785..5851) \ 
     CDS             complement(5600..5692)
                     /label=lacI (fragment)
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="lacI (fragment)" - database="snapgene" - identity="100.0" - match_length="8.6" - fragment="True" - other="CDS" - description="lac repressor; lacI; The lac repressor binds - to the lac operator to inhibit transcription in E. coli \ 
     repeat_region   5704..5715
                     /label=AAV2 ITR (fragment)
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="AAV2 ITR (fragment)" - database="itrs" - identity="100.0" - match_length="8.3" - fragment="True" - other="repeat_region" - description="AAV2 ITR Flip" -  \ 
                     /SerialCloner_Desc=complement(749..760) \ 
     promoter        3455..3476
                     /label=SV40 promoter (fragment)
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="SV40 promoter (fragment)" - database="snapgene" - identity="100.0" - match_length="6.1" - fragment="True" - other="promoter" - description="SV40 enhancer and early promoter" -  \ 
                     /SerialCloner_Desc=complement(2988..3009) \ 
ORIGIN
        1 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 
       61 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 
      121 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 
      181 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 
      241 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 
      301 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 
      361 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcgatctc ccgatcccct 
      421 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc 
      481 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca 
      541 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg 
      601 agctttgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt 
      661 ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc 
      721 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag 
      781 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg 
      841 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt 
      901 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg 
      961 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc 
     1021 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc 
     1081 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg 
     1141 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg 
     1201 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatcgatc 
     1261 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc 
     1321 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt 
     1381 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct 
     1441 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg 
     1501 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt 
     1561 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg 
     1621 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt 
     1681 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg 
     1741 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg 
     1801 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg 
     1861 tgtcgtgagg aattggcgaa gctcagttaa cggccattgt ggccatcaag cttggtacca 
     1921 gatctggatc cgctagcgcc gccaccatgg gctgcctcgg gaacagtaag accgaggacc 
     1981 agcgcaacga ggagaaggcg cagcgtgagg ccaacaaaaa gatcgagaag cagctgcaga 
     2041 gggacaagca ggtctaccgg gccacgcacc gcctgctcct tttgggcgct ggagaatctg 
     2101 gtaaaagcac cattgtgaag cagatgagga tcctgcatgt taatgggttt aatggagagg 
     2161 gcggcgaaga ggacccgcag gctgcaagga gcaacagcga tggtgagaag gcaaccaaag 
     2221 tgcaggacat caaaaacaac ctgaaagagg cgattgaaac cattgtggcc gccatgagca 
     2281 acctggtgcc ccccgtggag ctggccaacc ccgagaacca gttcagagtg gactacatcc 
     2341 tgagtgtgat gaacgtgcct gactttgact tccctcccga attctatgag catgccaagg 
     2401 ctctgtggga ggatgaagga gtgcgtgcct gctacgaacg ctccaacgag taccagctga 
     2461 ttgactgtgc ccagtacttc ctggacaaga tcgacgtgat caagcaggct gaatatatgc 
     2521 cgaccgagca ggacctgctt cgctgccgtg tcctgacttc tggaatcttt gagaccaagt 
     2581 tccaggtgga caaagtcaac ttccacatgt ttgacgtggg tggccagcgc gatgaacgcc 
     2641 gcaagtggat ccagtgcttc aacgatgtga ctgccatcat cttcgtggtg gccagcagca 
     2701 gctacaacat ggtcatccgg gaggacaacc agaccaaccg cctgcaggag gctctgaacc 
     2761 tcttcaagag catctggaac aacagatggc tgcgcaccat ctctgtgatc ctgttcctca 
     2821 acaagcaaga tctgctcgct gagaaagtcc ttgctgggaa atcgaagatt gaggactact 
     2881 ttccagaatt tgctcgctac actactcctg aggatgctac tcccgagccc ggagaggacc 
     2941 cacgcgtgac ccgggccaag tacttcattc gagatgagtt tctgaggatc agcactgcca 
     3001 gtggagatgg gcgtcactac tgctaccctc atttcacctg cgctgtggac actgagaaca 
     3061 tccgccgtgt gttcaacgac tgccgtgaca tcattcagcg catgcacctt cgtcagtacg 
     3121 agctgctcta ataggcggcc gcactcgaga tatcggcctc cagggcctct agagggccct 
     3181 attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt 
     3241 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact 
     3301 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat 
     3361 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc 
     3421 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc 
     3481 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 
     3541 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 
     3601 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 
     3661 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 
     3721 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 
     3781 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 
     3841 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 
     3901 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa 
     3961 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 
     4021 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 
     4081 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 
     4141 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 
     4201 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 
     4261 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat 
     4321 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 
     4381 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 
     4441 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 
     4501 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 
     4561 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 
     4621 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 
     4681 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 
     4741 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 
     4801 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 
     4861 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 
     4921 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 
     4981 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 
     5041 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 
     5101 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 
     5161 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 
     5221 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 
     5281 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 
     5341 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 
     5401 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 
     5461 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 
     5521 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 
     5581 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 
     5641 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 
     5701 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 
     5761 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 
     5821 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 
     5881 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 
     5941 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 
     6001 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 
     6061 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 
     6121 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 
     6181 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 
     6241 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 
     6301 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 
     6361 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 
     6421 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 
     6481 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 
     6541 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 
     6601 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 
     6661 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 
     6721 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
     6781 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
     6841 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
     6901 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 
     6961 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
     7021 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
     7081 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
     7141 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
     7201 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc cc
//