LOCUS       659_12A_pCEFL_SfiI__GNAS_EE_P116L.xdna    7242 bp      DNA     circular   11/6/2025
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: . - [LOCUS]: 12_RIB_12_12A_GNASEE_P116L_ - [AC
            CESSION]: urn.local...1e-ig17g7s
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     primer_bind     1893..1913
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     4181..4198
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      897..1525
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    1865..1887
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             86..745
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(2253..3044)
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        7060..7088
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        1831..1860
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    complement(3186..3263)
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      complement(3931..4158)
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    4251..5435
                     /label=GNASEE P116L
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 
       61 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 
      121 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 
      181 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 
      241 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 
      301 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 
      361 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 
      421 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 
      481 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 
      541 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 
      601 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 
      661 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 
      721 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 
      781 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 
      841 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 
      901 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 
      961 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 
     1021 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc 
     1081 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 
     1141 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 
     1201 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 
     1261 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 
     1321 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 
     1381 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 
     1441 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 
     1501 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 
     1561 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 
     1621 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 
     1681 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 
     1741 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 
     1801 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt 
     1861 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc 
     1921 caagctctag ctagaggtcg acggtataca gacatgataa gatacattga tgagtttgga 
     1981 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt 
     2041 gctttatttg taaccattat aagctgcaat aaacaagttg gggtgggcga agaactccag 
     2101 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc 
     2161 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc 
     2221 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag 
     2281 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc 
     2341 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg 
     2401 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg 
     2461 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc 
     2521 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca 
     2581 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct 
     2641 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc 
     2701 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact 
     2761 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa 
     2821 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg 
     2881 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac 
     2941 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc 
     3001 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct 
     3061 catcctgtct cttgatcaga tccgaaaatg gatatacaag ctcccgggag ctttttgcaa 
     3121 aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggcagaggcg 
     3181 gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa 
     3241 ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa 
     3301 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg 
     3361 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac 
     3421 tttccacacc ctaactgaca cacattccac agaattaatt cgcgttaaat ttttgttaaa 
     3481 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat 
     3541 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg 
     3601 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac 
     3661 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta 
     3721 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag 
     3781 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg 
     3841 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtgggga taccccctag 
     3901 agccccagct ggttctttcc gcctcagaag ccatagagcc caccgcatcc ccagcatgcc 
     3961 tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag 
     4021 aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg 
     4081 gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc 
     4141 aactagaagg cacagtcgag gctgatcagc gagctctagc atttaggtga cactatagaa 
     4201 tagggccctc tagaggccct ggaggccgat atctcgagtg cggccgccta ttagagcagc 
     4261 tcgtactgac gaaggtgcat gcgctgaatg atgtcacggc agtcgttgaa cacacggcgg 
     4321 atgttctcag tgtccacagc gcaggtgaaa tgagggtagc agtagtgacg cccatctcca 
     4381 ctggcagtgc tgatcctcag aaactcatct cgaatgaagt acttggcccg ggtcacgcgt 
     4441 gggtcctctc cgggctcggg agtagcatcc tcaggagtag tgtagcgagc aaattctgga 
     4501 aagtagtcct caatcttcga tttcccagca aggactttct cagcgagcag atcttgcttg 
     4561 ttgaggaaca ggatcacaga gatggtgcgc agccatctgt tgttccagat gctcttgaag 
     4621 aggttcagag cctcctgcag gcggttggtc tggttgtcct cccggatgac catgttgtag 
     4681 ctgctgctgg ccaccacgaa gatgatggca gtcacatcgt tgaagcactg gatccacttg 
     4741 cggcgttcat cgcgctggcc acccacgtca aacatgtgga agttgacttt gtccacctgg 
     4801 aacttggtct caaagattcc agaagtcagg acacggcagc gaagcaggtc ctgctcggtc 
     4861 ggcatatatt cagcctgctt gatcacgtcg atcttgtcca ggaagtactg ggcacagtca 
     4921 atcagctggt actcgttgga gcgttcgtag caggcacgca ctccttcatc ctcccacaga 
     4981 gccttggcat gctcatagaa ttcgggaggg aagtcaaagt caggcacgtt catcacactc 
     5041 aggatgtagt ccactctgaa ctggttctcg gggttggcca gctccacgag gggcaccagg 
     5101 ttgctcatgg cggccacaat ggtttcaatc gcctctttca ggttgttttt gatgtcctgc 
     5161 actttggttg ccttctcacc atcgctgttg ctccttgcag cctgcgggtc ctcttcgccg 
     5221 ccctctccat taaacccatt aacatgcagg atcctcatct gcttcacaat ggtgctttta 
     5281 ccagattctc cagcgcccaa aaggagcagg cggtgcgtgg cccggtagac ctgcttgtcc 
     5341 ttctgcagct gcttctcgat ctttttgttg gcctcacgct gcgccttctc ctcgttgcgc 
     5401 tggtcctcgg tcttactgtt cccgaggcag cccatggtgg cggcgctagc ggatccagat 
     5461 ctggtaccaa gcttgatggc cacaatggcc gttaactgag cttcgccaat tcctcacgac 
     5521 acctgaaatg gaagaaaaaa actttgaacc actgtctgag gcttgagaat gaaccaagat 
     5581 ccaaactcaa aaagggcaaa ttccaaggag aattacatca agtgccaagc tggcctaact 
     5641 tcagtctcca cccactcagt gtggggaaac tccatcgcat aaaacccctc cccccaacct 
     5701 aaagacgacg tactccaaaa gctcgagaac taatcgaggt gcctggacgg cgcccggtac 
     5761 tccgtggagt cacatgaagc gacggctgag gacggaaagg cccttttcct ttgtgtgggt 
     5821 gactcacccg cccgctctcc cgagcgccgc gtcctccatt ttgagctccc tgcagcaggg 
     5881 ccgggaagcg gccatctttc cgctcacgca actggtgccg accgggccag ccttgccgcc 
     5941 cagggcgggg cgatacacgg cggcgcgagg ccaggcacca gagcaggccg gccagcttga 
     6001 gactaccccc gtccgattct cggtggccgc gctcgcaggc cccgcctcgc cgaacatgtg 
     6061 cgctgggacg cacgggcccc gtcgccgccc gcggccccaa aaaccgaaat accagtgtgc 
     6121 agatcgatct tggcccgcat ttacaagact atcttgccag aaaaaaagcg tcgcagcagg 
     6181 tcatcaaaaa ttttaaatgg ctagagactt atcgaaagca gcgagacagg cgcgaaggtg 
     6241 ccaccagatt cgcacgcggc ggccccagcg cccaggccag gcctcaactc aagcacgagg 
     6301 cgaaggggct ccttaagcgc aaggcctcga actctcccac ccacttccaa cccgaagctc 
     6361 gggatcaaga atcacgtact gcagccaggt ggaagtaatt caaggcacgc aagggccata 
     6421 acccgtaaag aggccaggcc cgcgggaacc acacacggca cttacctgtg ttctggcggc 
     6481 aaacccgttg cgaaaaagaa cgttcacggc gactactgca cttatatacg gttctccccc 
     6541 accctcggga aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca 
     6601 ccttctctag gcaccggttc aattgccgac ccctcccccc aacttctcgg ggactgtggg 
     6661 cgatgtgcgc tctgcccact gacgggcacc ggagccaatt cccactcctt tcaagaccta 
     6721 gaaggtccat tagctgcaaa gattcctctc tgtttaaaac tttatccatc tttgcaaagc 
     6781 tcgaagcagc gcaaaacgcc taaccctaag cagattcttc atgcaattgt cggtcaagcc 
     6841 ttgccttgtt gtagcttaaa ttttgctcgc gcactactca gcgacctcca acacacaagc 
     6901 agggagcaga tactggctta actatgcggc atcagagcag attgtactga gagtgcacca 
     6961 taggggatcg ggagatcgat ctcccgatcc gtcgacgtca ggtggcactt ttcggggaaa 
     7021 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat 
     7081 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca 
     7141 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 
     7201 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag tt
//