LOCUS       660_13B_pCEFL_SfiI_GNASEE_N218I.xdna    7242 bp      DNA     circular   11/6/2025
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: . - [LOCUS]: 19_RIB_13 - [ACCESSION]: urn.loca
            l...2y-i40uqur
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     primer_bind     complement(5315..5335)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(3030..3047)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5703..6331)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(5341..5363)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(6483..7142)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4184..4975
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(140..168)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5368..5397)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3965..4042
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3070..3297
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 
       61 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 
      121 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 
      181 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga 
      241 tcgggagatc gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc 
      301 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg 
      361 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt 
      421 agggttaggc gttttgcgct gcttcgagct ttgcaaagat ggataaagtt ttaaacagag 
      481 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt 
      541 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 
      601 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 
      661 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 
      721 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt 
      781 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc 
      841 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg 
      901 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc 
      961 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag 
     1021 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt 
     1081 cttgtaaatg cgggccaaga tcgatctgca cactggtatt tcggtttttg gggccgcggg 
     1141 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg 
     1201 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct 
     1261 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc 
     1321 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg 
     1381 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc 
     1441 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt 
     1501 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt 
     1561 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc 
     1621 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt 
     1681 tcaaagtttt tttcttccat ttcaggtgtc gtgaggaatt ggcgaagctc agttaacggc 
     1741 cattgtggcc atcaagcttg gtaccagatc tggatccgct agcgccgcca ccatgggctg 
     1801 cctcgggaac agtaagaccg aggaccagcg caacgaggag aaggcgcagc gtgaggccaa 
     1861 caaaaagatc gagaagcagc tgcagaagga caagcaggtc taccgggcca cgcaccgcct 
     1921 gctccttttg ggcgctggag aatctggtaa aagcaccatt gtgaagcaga tgaggatcct 
     1981 gcatgttaat gggtttaatg gagagggcgg cgaagaggac ccgcaggctg caaggagcaa 
     2041 cagcgatggt gagaaggcaa ccaaagtgca ggacatcaaa aacaacctga aagaggcgat 
     2101 tgaaaccatt gtggccgcca tgagcaacct ggtgcccccc gtggagctgg ccaaccccga 
     2161 gaaccagttc agagtggact acatcctgag tgtgatgaac gtgcctgact ttgacttccc 
     2221 tcccgaattc tatgagcatg ccaaggctct gtgggaggat gaaggagtgc gtgcctgcta 
     2281 cgaacgctcc aacgagtacc agctgattga ctgtgcccag tacttcctgg acaagatcga 
     2341 cgtgatcaag caggctgaat atatgccgac cgagcaggac ctgcttcgct gccgtgtcct 
     2401 gacttctgga atctttgaga ccaagttcca ggtggacaaa gtcatcttcc acatgtttga 
     2461 cgtgggtggc cagcgcgatg aacgccgcaa gtggatccag tgcttcaacg atgtgactgc 
     2521 catcatcttc gtggtggcca gcagcagcta caacatggtc atccgggagg acaaccagac 
     2581 caaccgcctg caggaggctc tgaacctctt caagagcatc tggaacaaca gatggctgcg 
     2641 caccatctct gtgatcctgt tcctcaacaa gcaagatctg ctcgctgaga aagtccttgc 
     2701 tgggaaatcg aagattgagg actactttcc agaatttgct cgctacacta ctcctgagga 
     2761 tgctactccc gagcccggag aggacccacg cgtgacccgg gccaagtact tcattcgaga 
     2821 tgagtttctg aggatcagca ctgccagtgg agatgggcgt cactactgct accctcattt 
     2881 cacctgcgct gtggacactg agaacatccg ccgtgtgttc aacgactgcc gtgacatcat 
     2941 tcagcgcatg caccttcgtc agtacgagct gctctaatag gcggccgcac tcgagatatc 
     3001 ggcctccagg gcctctagag ggccctattc tatagtgtca cctaaatgct agagctcgct 
     3061 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc 
     3121 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg 
     3181 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca 
     3241 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt 
     3301 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg 
     3361 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 
     3421 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 
     3481 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 
     3541 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 
     3601 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 
     3661 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 
     3721 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg 
     3781 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 
     3841 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 
     3901 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 
     3961 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 
     4021 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg 
     4081 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat 
     4141 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 
     4201 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 
     4261 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 
     4321 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 
     4381 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 
     4441 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 
     4501 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 
     4561 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 
     4621 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 
     4681 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 
     4741 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 
     4801 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 
     4861 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 
     4921 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 
     4981 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 
     5041 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 
     5101 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt 
     5161 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 
     5221 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt 
     5281 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 
     5341 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 
     5401 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 
     5461 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 
     5521 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 
     5581 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 
     5641 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 
     5701 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 
     5761 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 
     5821 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 
     5881 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 
     5941 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 
     6001 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 
     6061 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 
     6121 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 
     6181 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 
     6241 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 
     6301 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 
     6361 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 
     6421 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 
     6481 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 
     6541 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 
     6601 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 
     6661 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 
     6721 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 
     6781 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 
     6841 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 
     6901 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 
     6961 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 
     7021 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 
     7081 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 
     7141 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 
     7201 agttcgatgt aacccactcg tgcacccaac tgatcttcag ca
//