LOCUS       661_pCEFL_SfiI__GNAS_EE_T350I.xdna    7242 bp      DNA     circular   11/6/2025
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=[DEFINITION]: . - [LOCUS]: cluster_001_consensus - [ACCESSIO
            N]: urn.local...w-ig14ywv
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    251..355
                     /label=AmpR promoter
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="-" - label="AmpR promoter" - 251..355 \ 
     misc_feature    418..470
                     /label=bom
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="-" - label="bom" - 418..470 \ 
     misc_feature    611..677
                     /label=small t antigen
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="+" - label="small t antigen" - 611..677 \ 
     misc_feature    915..1024
                     /label=EF-1? intron A
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="+" - label="EF-1? intron A" - 915..1024 \ 
     misc_feature    1654..1857
                     /label=EF-1? intron A
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="+" - label="EF-1? intron A" - 1654..1857 \ 
     misc_feature    3181..3199
                     /label=SP6 promoter
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="-" - label="SP6 promoter" - 3181..3199 \ 
     misc_feature    3225..3449
                     /label=bGH poly(A) signal
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="+" - label="bGH poly(A) signal" - 3225..3449 \ 
     misc_feature    3453..3474
                     /label=SV40 promoter
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="+" - label="SV40 promoter" - 3453..3474 \ 
     misc_feature    3495..3923
                     /label=f1 ori
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="+" - label="f1 ori" - 3495..3923 \ 
     misc_feature    3937..4266
                     /label=SV40 promoter
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="+" - label="SV40 promoter" - 3937..4266 \ 
     misc_feature    4117..4252
                     /label=SV40 ori
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="+" - label="SV40 ori" - 4117..4252 \ 
     misc_feature    4184..4252
                     /label=SELP_SV40
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="+" - label="SELP_SV40" - 4184..4252 \ 
     misc_feature    4333..5124
                     /label=neo
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="+" - label="neo" - 4333..5124 \ 
     misc_feature    5148..5306
                     /label=ble
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="+" - label="ble" - 5148..5306 \ 
     misc_feature    5301..5422
                     /label=SV40 poly(A) signal
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="+" - label="SV40 poly(A) signal" - 5301..5422 \ 
     misc_feature    5379..5429
                     /label=VP1_SV40
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="-" - label="VP1_SV40" - 5379..5429 \ 
     misc_feature    5493..5511
                     /label=T5 promoter
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="-" - label="T5 promoter" - 5493..5511 \ 
     misc_feature    5495..5511
                     /label=lac operator
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="-" - label="lac operator" - 5495..5511 \ 
     misc_feature    5504..5599
                     /label=penA
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="-" - label="penA" - 5504..5599 \ 
     misc_feature    5519..5549
                     /label=lac promoter
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="-" - label="lac promoter" - 5519..5549 \ 
     misc_feature    5564..5585
                     /label=CAP binding site
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="-" - label="CAP binding site" - 5564..5585 \ 
     misc_feature    5598..5690
                     /label=lacI
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="-" - label="lacI" - 5598..5690 \ 
     misc_feature    5873..6461
                     /label=ori
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="-" - label="ori" - 5873..6461 \ 
     misc_feature    6316..6420
                     /label=RNAI
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=Score="+" - label="RNAI" - 6316..6420 \ 
     misc_feature    1945..3129
                     /label=GNASEE T350I
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(5467..5487)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(3182..3199)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5855..6483)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(5493..5515)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(6635..52)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4336..5127
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(292..320)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5520..5549)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    4117..4194
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3222..3449
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 
       61 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 
      121 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 
      181 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 
      241 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 
      301 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 
      361 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctat 
      421 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg 
      481 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag 
      541 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgag 
      601 ctttgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct 
      661 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc 
      721 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt 
      781 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg 
      841 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg 
      901 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt 
      961 atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg 
     1021 agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc 
     1081 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc 
     1141 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac 
     1201 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatcgatctg 
     1261 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc 
     1321 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct 
     1381 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg 
     1441 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc 
     1501 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca 
     1561 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag 
     1621 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta 
     1681 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa 
     1741 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga 
     1801 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg 
     1861 tcgtgaggaa ttggcgaagc tcagttaacg gccattgtgg ccatcaagct tggtaccaga 
     1921 tctggatccg ctagcgccgc caccatgggc tgcctcggga acagtaagac cgaggaccag 
     1981 cgcaacgagg agaaggcgca gcgtgaggcc aacaaaaaga tcgagaagca gctgcagaag 
     2041 gacaagcagg tctaccgggc cacgcaccgc ctgctccttt tgggcgctgg agaatctggt 
     2101 aaaagcacca ttgtgaagca gatgaggatc ctgcatgtta atgggtttaa tggagagggc 
     2161 ggcgaagagg acccgcaggc tgcaaggagc aacagcgatg gtgagaaggc aaccaaagtg 
     2221 caggacatca aaaacaacct gaaagaggcg attgaaacca ttgtggccgc catgagcaac 
     2281 ctggtgcccc ccgtggagct ggccaacccc gagaaccagt tcagagtgga ctacatcctg 
     2341 agtgtgatga acgtgcctga ctttgacttc cctcccgaat tctatgagca tgccaaggct 
     2401 ctgtgggagg atgaaggagt gcgtgcctgc tacgaacgct ccaacgagta ccagctgatt 
     2461 gactgtgccc agtacttcct ggacaagatc gacgtgatca agcaggctga atatatgccg 
     2521 accgagcagg acctgcttcg ctgccgtgtc ctgacttctg gaatctttga gaccaagttc 
     2581 caggtggaca aagtcaactt ccacatgttt gacgtgggtg gccagcgcga tgaacgccgc 
     2641 aagtggatcc agtgcttcaa cgatgtgact gccatcatct tcgtggtggc cagcagcagc 
     2701 tacaacatgg tcatccggga ggacaaccag accaaccgcc tgcaggaggc tctgaacctc 
     2761 ttcaagagca tctggaacaa cagatggctg cgcaccatct ctgtgatcct gttcctcaac 
     2821 aagcaagatc tgctcgctga gaaagtcctt gctgggaaat cgaagattga ggactacttt 
     2881 ccagaatttg ctcgctacac tactcctgag gatgctactc ccgagcccgg agaggaccca 
     2941 cgcgtgaccc gggccaagta cttcattcga gatgagtttc tgaggatcag cattgccagt 
     3001 ggagatgggc gtcactactg ctaccctcat ttcacctgcg ctgtggacac tgagaacatc 
     3061 cgccgtgtgt tcaacgactg ccgtgacatc attcagcgca tgcaccttcg tcagtacgag 
     3121 ctgctctaat aggcggccgc actcgagata tcggcctcca gggcctctag agggccctat 
     3181 tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg 
     3241 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 
     3301 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 
     3361 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 
     3421 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc 
     3481 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 
     3541 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 
     3601 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 
     3661 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg 
     3721 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 
     3781 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta 
     3841 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 
     3901 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag 
     3961 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 
     4021 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 
     4081 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 
     4141 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 
     4201 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 
     4261 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga 
     4321 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg 
     4381 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg 
     4441 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc 
     4501 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct 
     4561 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa 
     4621 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg 
     4681 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa 
     4741 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat 
     4801 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg 
     4861 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc 
     4921 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac 
     4981 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg 
     5041 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc 
     5101 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag 
     5161 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg 
     5221 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 
     5281 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca 
     5341 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 
     5401 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg 
     5461 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 
     5521 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 
     5581 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 
     5641 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 
     5701 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 
     5761 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 
     5821 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 
     5881 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 
     5941 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 
     6001 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 
     6061 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 
     6121 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 
     6181 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 
     6241 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 
     6301 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 
     6361 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 
     6421 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 
     6481 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 
     6541 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 
     6601 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 
     6661 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 
     6721 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 
     6781 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 
     6841 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 
     6901 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 
     6961 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 
     7021 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 
     7081 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 
     7141 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 
     7201 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc gg
//