LOCUS       679_pLenti_X1_Puro_8xGLI_Luc-ren.txt.xdna    11592 bp      DNA     circular   11/6/2025
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=GateWay(tm) LR reaction between 675 pENTR1A PCR 8xGLI_v2_min
            Pro_Luc2p_Ren sequenced.txt.xdna as an Entry vector and 644 
            pLenti X1 Puro DEST addgene 17297.txt.xdna as a Destination 
            vector.
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     promoter        44..148
                     /label=AmpR promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=gene="bla" - label=AmpR promoter - 42..146 \ 
     CDS             149..1009
                     /label=confers resistance to ampicillin, carbenicillin, and_AmpR
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=codon_start=1 - gene="bla" - product="beta-lactamase" - label=AmpR - note="confers resistance to ampicillin, carbenicillin, and - related antibiotics" - translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI - ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQ \ 
     primer_bind     complement(367..386)
                     /label=Ampicillin resistance gene, reverse primer_Amp-R
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=Amp-R - note="Ampicillin resistance gene, reverse primer" - complement(365..384) \ 
     rep_origin      1180..1768
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of _ori
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=direction=RIGHT - label=ori - note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  - replication" - 1178..1766 \ 
     primer_bind     1669..1688
                     /label=pBR322 origin, forward primer_pBR322ori-F
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=pBR322ori-F - note="pBR322 origin, forward primer" - 1667..1686 \ 
     primer_bind     1922..1939
                     /label=L4440 vector, forward primer_L4440
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=L4440 - note="L4440 vector, forward primer" - 1920..1937 \ 
     protein_bind    2056..2077
                     /label=CAP binding activates transcription in the presence _CAP binding site
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=CAP binding site - bound_moiety="E. coli catabolite activator protein" - note="CAP binding activates transcription in the presence  - of cAMP." - 2054..2075 \ 
     promoter        2092..2122
                     /label=promoter for the E. coli lac operon_lac promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=lac promoter - note="promoter for the E. coli lac operon" - 2090..2120 \ 
     protein_bind    2130..2146
                     /label=The lac repressor binds to the lac operator to _lac operator
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=lac operator - bound_moiety="lac repressor encoded by lacI" - note="The lac repressor binds to the lac operator to  - inhibit transcription in E. coli. This inhibition can be  - relieved by adding lactose or  - isopropyl-beta-D-thiogalactopyranoside \ 
     primer_bind     2135..2157
                     /label=In lacZ gene_M13/pUC Reverse
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13/pUC Reverse - note="In lacZ gene" - 2133..2155 \ 
     primer_bind     2154..2170
                     /label=common sequencing primer, one of multiple similar _M13 rev
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13 rev - note="common sequencing primer, one of multiple similar  - variants" - 2152..2168 \ 
     primer_bind     2154..2170
                     /label=In lacZ gene. Also called M13-rev_M13 Reverse
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13 Reverse - note="In lacZ gene. Also called M13-rev" - 2152..2168 \ 
     primer_bind     2189..2209
                     /label=T3 promoter, forward primer_T3
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=T3 - note="T3 promoter, forward primer" - 2187..2207 \ 
     promoter        2191..2209
                     /label=promoter for bacteriophage T3 RNA polymerase_T3 promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=T3 promoter - note="promoter for bacteriophage T3 RNA polymerase" - 2189..2207 \ 
     promoter        2237..2463
                     /label=Rous sarcoma virus enhancer/promoter_RSV promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=RSV promoter - note="Rous sarcoma virus enhancer/promoter" - 2235..2461 \ 
     LTR             2464..2644
                     /label=truncated 5' long terminal repeat (LTR) from HIV-1_5' LTR (truncated)
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=5' LTR (truncated) - note="truncated 5' long terminal repeat (LTR) from HIV-1" - 2462..2642 \ 
     LTR             10400..10580
                     /label=truncated 5' long terminal repeat (LTR) from HIV-1_5' LTR (truncated)
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=5' LTR (truncated) - note="truncated 5' long terminal repeat (LTR) from HIV-1" - 2462..2642 \ 
     misc_feature    2691..2816
                     /label=packaging signal of human immunodeficiency virus _HIV-1 Psi
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=HIV-1 Psi - note="packaging signal of human immunodeficiency virus  - type 1" - 2689..2814 \ 
     misc_feature    3309..3542
                     /label=The Rev response element (RRE) of HIV-1 allows for _RRE
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=RRE - note="The Rev response element (RRE) of HIV-1 allows for  - Rev-dependent mRNA export from the nucleus to the  - cytoplasm." - 3307..3540 \ 
     CDS             3727..3771
                     /label=recognized by the 2H10 single-chain llama nanobody_gp41 peptide
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=codon_start=1 - product="antigenic peptide corresponding to amino acids  - 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik  - et al., 2013)" - label=gp41 peptide - note="recognized by the 2H10 single-chain llama nanobody" - translation="KNEQELLE \ 
     misc_feature    4038..4154
                     /label=central polypurine tract and central termination _cPPT/CTS
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=cPPT/CTS - note="central polypurine tract and central termination  - sequence of HIV-1" - 4036..4152 \ 
     misc_feature    8488..9076
                     /label=woodchuck hepatitis virus posttranscriptional _WPRE
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=WPRE - note="woodchuck hepatitis virus posttranscriptional  - regulatory element" - 5921..6509 \ 
     primer_bind     complement(8541..8561)
                     /label=WPRE, reverse primer_WPRE-R
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=WPRE-R - note="WPRE, reverse primer" - complement(5974..5994) \ 
     CDS             complement(8959..8970)
                     /label=Factor Xa site
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=codon_start=1 - product="Factor Xa recognition and cleavage site" - label=Factor Xa site - translation="IEGR" - complement(6392..6403) \ 
     promoter        9090..9589
                     /label=mouse phosphoglycerate kinase 1 promoter_PGK promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=PGK promoter - note="mouse phosphoglycerate kinase 1 promoter" - 6523..7022 \ 
     primer_bind     complement(9125..9148)
                     /label=Murine stem cell virus, reverse primer_MSCV-rev
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=MSCV-rev - note="Murine stem cell virus, reverse primer" - complement(6558..6581) \ 
     primer_bind     9522..9541
                     /label=Mouse PGK promoter, forward primer_mPGK-F
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=mPGK-F - note="Mouse PGK promoter, forward primer" - 6955..6974 \ 
     CDS             9610..10209
                     /label=confers resistance to puromycin_PuroR
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=codon_start=1 - gene="pac from Streptomyces alboniger" - product="puromycin N-acetyltransferase" - label=PuroR - note="confers resistance to puromycin" - translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER - VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAG \ 
     primer_bind     complement(9610..9629)
                     /label=Puromycin resistance gene, reverse primer. Also _Puro-R
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=Puro-R - note="Puromycin resistance gene, reverse primer. Also  - called puro-variant-R" - complement(7043..7062) \ 
     primer_bind     10106..10126
                     /label=Puromycin resistance gene, forward primer_Puro-F
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=Puro-F - note="Puromycin resistance gene, forward primer" - 7539..7559 \ 
     LTR             10347..10580
                     /label=self-inactivating 3' long terminal repeat (LTR) from_3' LTR (Delta-U3)
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=3' LTR (Delta-U3) - note="self-inactivating 3' long terminal repeat (LTR) from - HIV-1" - 7780..8013 \ 
     polyA_signal    10652..10773
                     /label=SV40 polyadenylation signal_SV40 poly(A) signal
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=SV40 poly(A) signal - note="SV40 polyadenylation signal" - 8085..8206 \ 
     primer_bind     6757..6776
                     /label=SV40 polyA, reverse primer_SV40pA-R
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=SV40pA-R - note="SV40 polyA, reverse primer" - complement(8122..8141) \ 
     primer_bind     10743..10762
                     /label=SV40 polyA terminator, reverse primer_EBV-rev
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=EBV-rev - note="SV40 polyA terminator, reverse primer" - 8176..8195 \ 
     rep_origin      10813..10948
                     /label=SV40 origin of replication_SV40 ori
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=SV40 ori - note="SV40 origin of replication" - 8246..8381 \ 
     primer_bind     complement(10968..10987)
                     /label=T7 promoter, forward primer_T7
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=T7 - note="T7 promoter, forward primer" - complement(8401..8420) \ 
     promoter        complement(10969..10987)
                     /label=promoter for bacteriophage T7 RNA polymerase_T7 promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=T7 promoter - note="promoter for bacteriophage T7 RNA polymerase" - complement(8402..8420) \ 
     primer_bind     complement(10997..11014)
                     /label=In lacZ gene. Also called M13-F20 or M13 (-21) _M13 Forward
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13 Forward - note="In lacZ gene. Also called M13-F20 or M13 (-21)  - Forward" - complement(8430..8447) \ 
     primer_bind     complement(10997..11013)
                     /label=common sequencing primer, one of multiple similar _M13 fwd
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13 fwd - note="common sequencing primer, one of multiple similar  - variants" - complement(8430..8446) \ 
     primer_bind     complement(11006..11028)
                     /label=In lacZ gene_M13/pUC Forward
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=M13/pUC Forward - note="In lacZ gene" - complement(8439..8461) \ 
     rep_origin      11155..11583
                     /label=f1 bacteriophage origin of replication; arrow _f1 ori
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=direction=RIGHT - label=f1 ori - note="f1 bacteriophage origin of replication; arrow  - indicates direction of (+) strand synthesis" - 8588..9016 \ 
     primer_bind     complement(11242..11261)
                     /label=F1 origin, reverse primer_F1ori-R
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=F1ori-R - note="F1 origin, reverse primer" - complement(8675..8694) \ 
     primer_bind     11452..11473
                     /label=F1 origin, forward primer_F1ori-F
                     /SerialCloner_Color=&h808000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=label=F1ori-F - note="F1 origin, forward primer" - 8885..8906 \ 
     CDS             7395..8327
                     /label=LUCI_RENRE
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="LUCI_RENRE" - database="swissprot" - identity="100.0" - match_length="100.0" - fragment="False" - other="CDS" - 4291..5223 \ 
     CDS             4868..6517
                     /label=luciferase
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="luciferase" - database="snapgene" - identity="100.0" - match_length="99.8" - fragment="False" - other="CDS" - 1764..3413 \ 
     polyA_signal    10652..10773
                     /label=SV40 poly(A) signal
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="SV40 poly(A) signal" - database="snapgene" - identity="100.0" - match_length="100.0" - fragment="False" - other="polyA_signal" - complement(3588..3709) \ 
     CDS             6521..6640
                     /label=hPEST
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="hPEST" - database="snapgene" - identity="100.0" - match_length="100.0" - fragment="False" - other="CDS" - 3417..3536 \ 
     misc_feature    4339..4430
                     /label=pause site
                     /SerialCloner_Color=&hDC143C
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="pause site" - database="snapgene" - identity="100.0" - match_length="100.0" - fragment="False" - other="misc_feature" - 1235..1326 \ 
     polyA_signal    4277..4325
                     /label=poly(A) signal
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="poly(A) signal" - database="snapgene" - identity="100.0" - match_length="100.0" - fragment="False" - other="polyA_signal" - 5250..5298 \ 
     polyA_signal    8354..8402
                     /label=poly(A) signal
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="poly(A) signal" - database="snapgene" - identity="100.0" - match_length="100.0" - fragment="False" - other="polyA_signal" - 5250..5298 \ 
     promoter        7007..7364
                     /label=SV40 promoter
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="SV40 promoter" - database="snapgene" - identity="99.7" - match_length="100.0" - fragment="False" - other="promoter" - 3903..4260 \ 
     promoter        4804..4835
                     /label=minP
                     /SerialCloner_Color=&hF08080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="minP" - database="snapgene" - identity="100.0" - match_length="100.0" - fragment="False" - other="promoter" - 1700..1731 \ 
     CDS             complement(6839..6970)
                     /label=LT_SV40 (fragment)
                     /SerialCloner_Color=&hFF4500
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=note="pLannotate" - label="LT_SV40 (fragment)" - database="swissprot" - identity="59.6" - match_length="6.2" - fragment="True" - other="CDS" - complement(3735..3866) \ 
ORIGIN
        1 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 
       61 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 
      121 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 
      181 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 
      241 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 
      301 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 
      361 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 
      421 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 
      481 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 
      541 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 
      601 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 
      661 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 
      721 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 
      781 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 
      841 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 
      901 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 
      961 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 
     1021 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 
     1081 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 
     1141 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 
     1201 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 
     1261 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 
     1321 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 
     1381 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 
     1441 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 
     1501 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 
     1561 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 
     1621 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 
     1681 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 
     1741 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 
     1801 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 
     1861 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 
     1921 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc 
     1981 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 
     2041 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt 
     2101 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 
     2161 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct 
     2221 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg 
     2281 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa 
     2341 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga 
     2401 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata 
     2461 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 
     2521 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 
     2581 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 
     2641 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 
     2701 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 
     2761 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 
     2821 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 
     2881 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 
     2941 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 
     3001 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 
     3061 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 
     3121 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga 
     3181 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa 
     3241 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca 
     3301 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca 
     3361 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag 
     3421 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc 
     3481 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc 
     3541 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 
     3601 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac 
     3661 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag 
     3721 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg 
     3781 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg 
     3841 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat 
     3901 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga 
     3961 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct 
     4021 cgacggtatc ggttaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa 
     4081 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca 
     4141 aaattcaaaa ttttatcgtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca 
     4201 aaaaagcagg ctttaaagga accaattcag tcgactggat ccggtaccga attcgcggcc 
     4261 gcattggaca ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt 
     4321 gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact 
     4381 agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct ggcctaactg 
     4441 gccggtacac gcgtagattc gcgatcgacc tcgacaagca gggaacaccc aagtagaagt 
     4501 tccgacaagc agggaacacc caagtagaag ctctcgacct cgacaagcag ggaacaccca 
     4561 agtagaagct ccgacaagca gggaacaccc aagtagaagc tctcgacctc gacaagcagg 
     4621 gaacacccaa gtagaagctc cgacaagcag ggaacaccca agtagaagct ctcgacctcg 
     4681 acaagcaggg aacacccaag tagaagctcc gacaagcagg gaacacccaa gtagaagctc 
     4741 tcgatcgcga atctgtggta ccgaggatat caagatctgg cctcggcggc caagcttaga 
     4801 cactagaggg tatataatgg aagctcgact tccagcttgg caatccggta ctgttggtaa 
     4861 agccaccatg gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga 
     4921 agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg 
     4981 caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga 
     5041 gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat 
     5101 cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat 
     5161 cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat 
     5221 gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa 
     5281 cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta 
     5341 ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga 
     5401 gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag 
     5461 tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg 
     5521 attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct 
     5581 cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg 
     5641 cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca 
     5701 agactataag attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag 
     5761 cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc 
     5821 gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca 
     5881 gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa 
     5941 gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac 
     6001 cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat 
     6061 gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct 
     6121 gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct 
     6181 gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct 
     6241 gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg 
     6301 cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat 
     6361 cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt 
     6421 cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat 
     6481 tctcattaag gccaagaagg gcggcaagat cgccgtgaat tctcacggct tccctcccga 
     6541 ggtggaggag caggccgccg gcaccctgcc catgagctgc gcccaggaga gcggcatgga 
     6601 tagacaccct gctgcttgcg ccagcgccag gatcaacgtc taaggccgcg actctagagt 
     6661 cggggcggcc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc 
     6721 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta 
     6781 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg 
     6841 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt 
     6901 ggtaaaatcg ataaggatcc gtttgcgtat tgggcgctct tccgctgatc tgcgcagcac 
     6961 catggcctga aataacctct gaaagaggaa cttggttagc taccttctga ggcggaaaga 
     7021 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca 
     7081 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 
     7141 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 
     7201 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 
     7261 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc 
     7321 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc gattcttctg 
     7381 acactagcgc caccatgacc agcaaggtgt acgaccccga gcagaggaag aggatgatca 
     7441 ccggccccca gtggtgggcc aggtgcaagc agatgaacgt gctggacagc ttcatcaact 
     7501 actacgacag cgagaagcac gccgagaacg ccgtgatctt cctgcacggc aacgccgcta 
     7561 gcagctacct gtggaggcac gtggtgcccc acatcgagcc cgtggccagg tgcatcatcc 
     7621 ccgatctgat cggcatgggc aagagcggca agagcggcaa cggcagctac aggctgctgg 
     7681 accactacaa gtacctgacc gcctggttcg agctcctgaa cctgcccaag aagatcatct 
     7741 tcgtgggcca cgactggggc gcctgcctgg ccttccacta cagctacgag caccaggaca 
     7801 agatcaaggc catcgtgcac gccgagagcg tggtggacgt gatcgagagc tgggacgagt 
     7861 ggccagacat cgaggaggac atcgccctga tcaagagcga ggagggcgag aagatggtgc 
     7921 tggagaacaa cttcttcgtg gagaccatgc tgcccagcaa gatcatgaga aagctggagc 
     7981 ccgaggagtt cgccgcctac ctggagccct tcaaggagaa gggcgaggtg agaagaccca 
     8041 ccctgagctg gcccagagag atccccctgg tgaagggcgg caagcccgac gtggtgcaga 
     8101 tcgtgagaaa ctacaacgcc tacctgagag ccagcgacga cctgcccaag atgttcatcg 
     8161 agagcgaccc cggcttcttc agcaacgcca tcgtggaggg cgccaagaag ttccccaaca 
     8221 ccgagttcgt gaaggtgaag ggcctgcact tcagccagga ggacgccccc gacgagatgg 
     8281 gcaagtacat caagagcttc gtggagagag tgctgaagaa cgagcagtaa actctagaac 
     8341 cggtcatggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg 
     8401 tgttcgaact agatgctgct cgagatatct agacccagct ttcttgtaca aagtggttga 
     8461 tatccagcac agtggcggcc gctcgacaat caacctctgg attacaaaat ttgtgaaaga 
     8521 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 
     8581 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 
     8641 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 
     8701 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 
     8761 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 
     8821 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 
     8881 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 
     8941 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 
     9001 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 
     9061 tgggccgcct ccccgcctgg aattctaccg ggtaggggag gcgcttttcc caaggcagtc 
     9121 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc 
     9181 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt 
     9241 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc 
     9301 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac 
     9361 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt 
     9421 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct 
     9481 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa 
     9541 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc 
     9601 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 
     9661 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 
     9721 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 
     9781 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 
     9841 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 
     9901 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg 
     9961 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 
    10021 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 
    10081 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 
    10141 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 
    10201 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat 
    10261 gcatctcgag ggcccggtac ctttaagacc aatgacttac aaggcagctg tagatcttag 
    10321 ccacttttta aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga 
    10381 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc 
    10441 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt 
    10501 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc 
    10561 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact 
    10621 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta 
    10681 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 
    10741 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc 
    10801 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 
    10861 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 
    10921 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt 
    10981 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 
    11041 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 
    11101 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc 
    11161 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 
    11221 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 
    11281 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 
    11341 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 
    11401 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 
    11461 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 
    11521 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 
    11581 attttaacaa aa
//