LOCUS       pMX_H2B_mKate2          5612 bp    DNA        circular     07-NOV-2025
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     >pMXs, 5822 bp
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    3092..3643
                     /locus_tag="5'LTR2"
     misc_feature    52..600
                     /locus_tag="5' LTR2"
     misc_feature    2280..2972
                     /locus_tag="mKate2"
     misc_feature    1072..1566
                     /locus_tag="gag"
     rep_origin      complement(3862..4544)
                     /locus_tag="ColE1 origin"
     CDS             complement(4642..5301)
                     /locus_tag="AmpR"
     misc_feature    1896..2273
                     /locus_tag="H2B"
ORIGIN
        1 ctgcataatg aaagacccca cctgtaggtt tggcaagcta gcttaagtnn naacgccatt
       61 ttgcaaggca tgggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa
      121 cagagaaaca ggagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgc
      181 tcagggccaa gaacagttgg aacaggagaa ttgggccaaa caggatatct gtggtaagca
      241 gttcctgccc cgctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
      301 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
      361 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
      421 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
      481 cgcccgggta cccgtgtatc caataaagcc tcttgctgtt tgcatccgac ttgtggtctc
      541 gctgttcctt gggagggtct cctctgagtg attgactacc cacgacgggg gtctttcatt
      601 tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag
      661 gtaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat gtttgatgtt
      721 atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga
      781 actgacgagt tctgaacacc cggccgcaac cctgggagac gtcccaggtc gggggccgtt
      841 tttgtggccc gacctgagtc caaaaatccc gatcgttttg gactctttgg tgcacccccc
      901 ttagaggagg gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg
      961 tctgaatttt tgctttcggt ttgggaccga agccgcgccg cgcgtcttgt ctgctgcagc
     1021 atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa tatgggcccg
     1081 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
     1141 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
     1201 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
     1261 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
     1321 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
     1381 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
     1441 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccccc
     1501 atatggccat atgagatctt atatggggca cccccgcccc ttgtaaactt ccctgaccct
     1561 gacatgacaa gagttactaa cagcccctct ctccaagctc acttacaggc tctctactta
     1621 gtccagcacg aagtctggag acctctggcg gcagcctacc aagaacaact ggaccgaccg
     1681 gtggtacctc acccttaccg agtcggcgac acagtgtggg tccgccgaca ccagactaag
     1741 aacctagaac ctcgctggaa aggaccttac acagtcctgc tgaccacccc caccgccctc
     1801 aaagtagacg gcatcgcagc ttggatacac gccgcccacg tgaaggctgc cgaccccggg
     1861 ggtggaccat cctctagact gccggatccg ccaccATGCC GGAGCCAGCG AAGTCTGCTC
     1921 CCGCGCCCAA GAAGGGCTCG AAGAAAGCCG TGACTAAGGC GCAGAAGAAG GACGGCAAGA
     1981 AGCGCAAGCG CAGCCGCAAG GAGAGCTACT CCGTATACGT GTACAAGGTG CTGAAGCAGG
     2041 TCCACCCCGA CACCGGCATC TCCTCTAAGG CCATGGGAAT CATGAACTCC TTCGTCAACG
     2101 ACATCTTCGA ACGCATCGCG GGTGAGGCTT CCCGCCTGGC GCATTACAAC AAGCGCTCGA
     2161 CCATCACCTC CAGGGAGATC CAGACGGCCG TGCGCCTGCT GCTGCCCGGG GAGTTGGCCA
     2221 AGCACGCCGT GTCCGAGGGC ACCAAGGCCG TCACCAAGTA CACCAGCGCT AAGgaattcG
     2281 TGAGCGAGCT GATTAAGGAG AACATGCACA TGAAGCTGTA CATGGAGGGC ACCGTGAACA
     2341 ACCACCACTT CAAGTGCACA TCCGAGGGCG AAGGCAAGCC CTACGAGGGC ACCCAGACCA
     2401 TGAGAATCAA GGCGGTCGAG GGCGaCCCTC TCCCCTTCGC CTTCGACATC CTGGCTACCA
     2461 GCTTCATGTA CGGCAGCAAA ACCTTCATCA ACCACACCCA GGGCATCCCC GACTTCTTTA
     2521 AGCAGTCCTT CCCCGAGGGC TTCACATGGG AGAGAGTCAC CACATACGAA GACGGGGGCG
     2581 TGCTGACCGC TACCCAGGAC ACCAGCCTCC AGGACGGCTG CCTCATCTAC AACGTCAAGA
     2641 TCAGAGGGGT GAACTTCCCA TCCAACGGCC CTGTGATGCA GAAGAAAACA CTCGGCTGGG
     2701 AGGCCTCCAC CGAGACCCTG TACCCCGCTG ACGGCGGCCT GGAAGGCAGA GCCGACATGG
     2761 CCCTGAAGCT CGTGGGCGGG GGCCACCTGA TCTGCAACTT GAAGACCACA TACAGATCCA
     2821 AGAAACCCGC TAAGAACCTC AAGATGCCCG GCGTCTACTA TGTGGACAGA AGACTGGAAA
     2881 GAATCAAGGA GGCCGACAAA GAGACCTACG TCGAGCAGCA CGAGGTGGCT GTGGCCAGAT
     2941 ACTGCGACCT CCCTAGCAAA CTGGGGCACA GAggcagcct cgagcggccg ccagcacagt
     3001 ggtcgacgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     3061 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttggaaggca tggaaaaata
     3121 cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg
     3181 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     3241 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     3301 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc
     3361 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
     3421 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
     3481 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
     3541 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
     3601 ctcctctgag tgattgacta cccgtcagcg ggggtctttc attctgcatt aatgaatcgg
     3661 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3721 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3781 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3841 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3901 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3961 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4021 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4081 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4141 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4201 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4261 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4321 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4381 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4441 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4501 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4561 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4621 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4681 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4741 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     4801 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4861 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     4921 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     4981 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5041 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5101 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5161 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5221 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     5281 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5341 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5401 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5461 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5521 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5581 aaataaacaa ataggggttc cgcgcacatt tc  
//