LOCUS       pMX_Bmal1Pro_mVe        7675 bp    DNA        circular     07-NOV-2025
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     >pMXs, 5822 bp
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    5155..5706
                     /locus_tag="5'LTR2"
     misc_feature    1890..2389
                     /locus_tag="Bmal1promoter"
     misc_feature    2402..2416
                     /locus_tag="vec_inv"
     misc_feature    52..600
                     /locus_tag="5' LTR2"
     misc_feature    3104..3118
                     /locus_tag="Vec_Fwd"
     misc_feature    2402..3118
                     /locus_tag="Venus"
     misc_feature    1072..1566
                     /locus_tag="gag"
     rep_origin      complement(5925..6607)
                     /locus_tag="ColE1 origin"
     CDS             complement(6705..7364)
                     /locus_tag="AmpR"
     misc_feature    3161..5041
                     /locus_tag="BMAL1"
ORIGIN
        1 ctgcataatg aaagacccca cctgtaggtt tggcaagcta gcttaagtnn naacgccatt
       61 ttgcaaggca tgggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa
      121 cagagaaaca ggagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgc
      181 tcagggccaa gaacagttgg aacaggagaa ttgggccaaa caggatatct gtggtaagca
      241 gttcctgccc cgctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
      301 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
      361 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
      421 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
      481 cgcccgggta cccgtgtatc caataaagcc tcttgctgtt tgcatccgac ttgtggtctc
      541 gctgttcctt gggagggtct cctctgagtg attgactacc cacgacgggg gtctttcatt
      601 tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag
      661 gtaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat gtttgatgtt
      721 atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga
      781 actgacgagt tctgaacacc cggccgcaac cctgggagac gtcccaggtc gggggccgtt
      841 tttgtggccc gacctgagtc caaaaatccc gatcgttttg gactctttgg tgcacccccc
      901 ttagaggagg gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg
      961 tctgaatttt tgctttcggt ttgggaccga agccgcgccg cgcgtcttgt ctgctgcagc
     1021 atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa tatgggcccg
     1081 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
     1141 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
     1201 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
     1261 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
     1321 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
     1381 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
     1441 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccccc
     1501 atatggccat atgagatctt atatggggca cccccgcccc ttgtaaactt ccctgaccct
     1561 gacatgacaa gagttactaa cagcccctct ctccaagctc acttacaggc tctctactta
     1621 gtccagcacg aagtctggag acctctggcg gcagcctacc aagaacaact ggaccgaccg
     1681 gtggtacctc acccttaccg agtcggcgac acagtgtggg tccgccgaca ccagactaag
     1741 aacctagaac ctcgctggaa aggaccttac acagtcctgc tgaccacccc caccgccctc
     1801 aaagtagacg gcatcgcagc ttggatacac gccgcccacg tgaaggctgc cgaccccggg
     1861 ggtggaccat cctctagact gccggatccg ggacgacggc gagctcgcag agtccgcaac
     1921 gcagtggcct cagcgagctt tagacctgag ggggaaaacc gagggctggg gcaagaaatc
     1981 cacagagcgt gccaattggt ccactcctcg gggcgtgtgc ttctgtgcgc caaatgattg
     2041 gtgaagggaa agtagcaggt aaaccagccc tgccgtcttg ccattggtca gaggctttcc
     2101 tatcggtcac tcgattggct agcctaacgc agagcagaac gcgaattggt ttgggttgtc
     2161 cgccaagaca actccgttcg ctctctctga ttggctaacg ggaagaggca ggtatccggg
     2221 ctgcgcggct cctccattgg tgggcgggga aggggggttg ggcacagcga ttggtgggcg
     2281 gggggccggg cctgggccgg cggggagcgg attggtcgga aagtaggtta gtggtgcgac
     2341 atttagggaa ggcagaaagt aggtcaggga cggaggtgcc tgtttaccca gatctgccac
     2401 cATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
     2461 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA
     2521 CGGCAAGCTG ACCCTGAAGC TGATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
     2581 CCTCGTGACC ACCCTGGGCT ACGGCCTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA
     2641 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT
     2701 CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT
     2761 GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA
     2821 CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA
     2881 CGGCATCAAG GCCAACTTCA AGATCCGCCA CAACATCGAG GACGGCGGCG TGCAGCTCGC
     2941 CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA
     3001 CTACCTGAGC TACCAGTCCa aaCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT
     3061 CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGgg
     3121 ctccgctggc tccgctgctg gttctggcga atttgaattc ATGGCGGACC AGAGAATGGA
     3181 CATTTCCTCA ACCATCAGCG ACTTCATGTC TCCGGGCCCC ACCGACCTAC TCTCCGGTTC
     3241 CCTGGGCACC AGTGGTGTGG ACTGCAATCG CAAGAGGAAA GGCAGTGCCA CTGACTACCA
     3301 AGAAAGTATG GACACAGACA AAGATGACCC TCATGGAAGG TTAGAATATG CAGAACACCA
     3361 AGGAAGGATC AAGAATGCAA GGGAGGCCCA CAGTCAGATT GAAAAGAGGC GTCGGGACAA
     3421 AATGAACAGT TTCATTGATG AATTGGCTTC TTTGGTACCA ACATGCAATG CAATGTCCAG
     3481 GAAGTTAGAT AAACTCACCG TGCTAAGGAT GGCTGTTCAG CACATGAAAA CTTTGAGAGG
     3541 TGCCACCAAC CCATACACAG AAGCAAACTA CAAGCCAACA TTTCTATCAG ATGACGAACT
     3601 GAAACACCTA ATTCTCAGGG CAGCAGATGG ATTTTTGTTT GTCGTAGGAT GTGACCGAGG
     3661 GAAGATCCTC TTTGTCTCCG AGTCTGTCTT CAAGATCCTC AATTATAGCC AGAATGACCT
     3721 TATTGGCCAG AGCTTGTTTG ACTACCTGCA TCCAAAAGAT ATTGCCAAAG TTAAGGAACA
     3781 GCTATCTTCC TCGGACACTG CGCCCCGGGA GCGACTCATT GATGCCAAGA CTGGACTTCC
     3841 GGTTAAAACG GATATAACCC CTGGGCCCTC CCGGCTATGC TCTGGAGCCC GCCGCTCTTT
     3901 CTTCTGTAGA ATGAAGTGCA ACAGGCCATC AGTAAAGGTG GAAGATAAGG ACTTCGCCTC
     3961 TACCTGTTCA AAGAAAAAAG CAGATCGAAA AAGCTTCTGC ACAATCCACA GCACAGGCTA
     4021 TTTGAAAAGC TGGCCACCCA CGAAGATGGG GCTGGACGAA GACAATGAGC CAGACAACGA
     4081 GGGCTGCAAC CTCAGCTGCC TCGTTGCAAT CGGGCGCCTG CACTCGCACA TGGTTCCACA
     4141 ACCAGCGAAC GGGGAAATAC GGGTGAAATC TATGGAGTAC GTTTCTCGAC ACGCAATAGA
     4201 TGGGAAATTT GTTTTTGTAG ATCAGAGGGC GACAGCTATT TTGGCGTATC TACCACAGGA
     4261 ACTTCTAGGT ACATCATGTT ATGAGTATTT TCATCAAGAC GACATAGGAC ACCTCGCAGA
     4321 ATGTCACAGG CAAGTTTTAC AGACAAGAGA AAAGATCACA ACTAATTGCT ATAAGTTTAA
     4381 GATCAAAGAT GGTTCTTTTA TCACGCTACG AAGTCGATGG TTCAGTTTCA TGAACCCGTG
     4441 GACCAAGGAA GTTGAATACA TTGTCTCAAC CAACACTGTT GTTTTAGCCA ATGTCCTGGA
     4501 AGGCGGGGAC CCAACCTTCC CGCAGCTAAC AGCACCCCCC CACAGCATGG ACAGCATGCT
     4561 GCCCTCTGGA GAAGGTGGCC CAAAGAGGAC TCATCCCACT GTTCCAGGCA TTCCAGGGGG
     4621 AACCAGAGCC GGAGCAGGAA AAATAGGTCG AATGATTGCC GAGGAAATCA TGGAAATCCA
     4681 CAGGATAAGA GGGTCATCGC CTTCCAGCTG TGGCTCCAGC CCGCTGAACA TCACAAGTAC
     4741 GCCTCCCCCT GATGCCCCTT CTCCAGGAGG CAAGAAGATT CTAAATGGAG GGACTCCAGA
     4801 CATTCCTTCC ACTGGACTAT TACCAGGGCA GGCTCAGGAG ACCCCAGGGT ATCCCTATTC
     4861 TGATAGTTCT TCTATTCTTG GTGAGAACCC CCACATAGGC ATCGATATGA TAGATAACGA
     4921 CCAAGGATCA AGTAGTCCCA GTAACGATGA GGCAGCAATG GCTGTCATCA TGAGCCTCTT
     4981 GGAAGCAGAT GCGGGGCTGG GTGGCCCCGT TGACTTTAGT GACTTGCCAT GGCCGCTGTA
     5041 Gctcgagcgg ccgccagcac agtggtcgac gataaaataa aagattttat ttagtctcca
     5101 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
     5161 attttggaag gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg
     5221 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     5281 cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
     5341 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc
     5401 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     5461 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     5521 tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga
     5581 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
     5641 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     5701 ttcattctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5761 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5821 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5881 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5941 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6001 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6061 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6121 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6181 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6241 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6301 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6361 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     6421 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6481 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6541 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6601 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6661 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6721 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6781 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6841 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6901 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6961 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7021 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7081 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7141 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7201 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7261 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
     7321 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7381 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7441 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7501 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7561 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7621 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttc
//