LOCUS       pMX_Bmal1Pro_mVe        7693 bp    DNA        circular     07-NOV-2025
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     >pMXs, 5822 bp
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    5173..5724
                     /locus_tag="5'LTR2"
     misc_feature    1890..2389
                     /locus_tag="Bmal1promoter"
     misc_feature    2402..2416
                     /locus_tag="vec_inv"
     misc_feature    52..600
                     /locus_tag="5' LTR2"
     misc_feature    3104..3118
                     /locus_tag="Vec_Fwd"
     misc_feature    2402..3118
                     /locus_tag="Venus"
     misc_feature    1072..1566
                     /locus_tag="gag"
     rep_origin      complement(5943..6625)
                     /locus_tag="ColE1 origin"
     CDS             complement(6723..7382)
                     /locus_tag="AmpR"
     misc_feature    3179..5059
                     /locus_tag="BMAL1"
ORIGIN
        1 ctgcataatg aaagacccca cctgtaggtt tggcaagcta gcttaagtnn naacgccatt
       61 ttgcaaggca tgggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa
      121 cagagaaaca ggagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgc
      181 tcagggccaa gaacagttgg aacaggagaa ttgggccaaa caggatatct gtggtaagca
      241 gttcctgccc cgctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
      301 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
      361 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
      421 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
      481 cgcccgggta cccgtgtatc caataaagcc tcttgctgtt tgcatccgac ttgtggtctc
      541 gctgttcctt gggagggtct cctctgagtg attgactacc cacgacgggg gtctttcatt
      601 tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag
      661 gtaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat gtttgatgtt
      721 atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga
      781 actgacgagt tctgaacacc cggccgcaac cctgggagac gtcccaggtc gggggccgtt
      841 tttgtggccc gacctgagtc caaaaatccc gatcgttttg gactctttgg tgcacccccc
      901 ttagaggagg gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg
      961 tctgaatttt tgctttcggt ttgggaccga agccgcgccg cgcgtcttgt ctgctgcagc
     1021 atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa tatgggcccg
     1081 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
     1141 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
     1201 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
     1261 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
     1321 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
     1381 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
     1441 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccccc
     1501 atatggccat atgagatctt atatggggca cccccgcccc ttgtaaactt ccctgaccct
     1561 gacatgacaa gagttactaa cagcccctct ctccaagctc acttacaggc tctctactta
     1621 gtccagcacg aagtctggag acctctggcg gcagcctacc aagaacaact ggaccgaccg
     1681 gtggtacctc acccttaccg agtcggcgac acagtgtggg tccgccgaca ccagactaag
     1741 aacctagaac ctcgctggaa aggaccttac acagtcctgc tgaccacccc caccgccctc
     1801 aaagtagacg gcatcgcagc ttggatacac gccgcccacg tgaaggctgc cgaccccggg
     1861 ggtggaccat cctctagact gccggatccg ggacgacggc gagctcgcag agtccgcaac
     1921 gcagtggcct cagcgagctt tagacctgag ggggaaaacc gagggctggg gcaagaaatc
     1981 cacagagcgt gccaattggt ccactcctcg gggcgtgtgc ttctgtgcgc caaatgattg
     2041 gtgaagggaa agtagcaggt aaaccagccc tgccgtcttg ccattggtca gaggctttcc
     2101 tatcggtcac tcgattggct agcctaacgc agagcagaac gcgaattggt ttgggttgtc
     2161 cgccaagaca actccgttcg ctctctctga ttggctaacg ggaagaggca ggtatccggg
     2221 ctgcgcggct cctccattgg tgggcgggga aggggggttg ggcacagcga ttggtgggcg
     2281 gggggccggg cctgggccgg cggggagcgg attggtcgga aagtaggtta gtggtgcgac
     2341 atttagggaa ggcagaaagt aggtcaggga cggaggtgcc tgtttaccca gatctgccac
     2401 cATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA
     2461 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA
     2521 CGGCAAGCTG ACCCTGAAGC TGATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC
     2581 CCTCGTGACC ACCCTGGGCT ACGGCCTGCA GTGCTTCGCC CGCTACCCCG ACCACATGAA
     2641 GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT
     2701 CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT
     2761 GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA
     2821 CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ACCGCCGACA AGCAGAAGAA
     2881 CGGCATCAAG GCCAACTTCA AGATCCGCCA CAACATCGAG GACGGCGGCG TGCAGCTCGC
     2941 CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA
     3001 CTACCTGAGC TACCAGTCCa aaCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT
     3061 CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGgg
     3121 tggctctggc ggaagcggct ccgctggctc tgctgccgga tctggcgagt ttgaattcAT
     3181 GGCGGACCAG AGAATGGACA TTTCCTCAAC CATCAGCGAC TTCATGTCTC CGGGCCCCAC
     3241 CGACCTACTC TCCGGTTCCC TGGGCACCAG TGGTGTGGAC TGCAATCGCA AGAGGAAAGG
     3301 CAGTGCCACT GACTACCAAG AAAGTATGGA CACAGACAAA GATGACCCTC ATGGAAGGTT
     3361 AGAATATGCA GAACACCAAG GAAGGATCAA GAATGCAAGG GAGGCCCACA GTCAGATTGA
     3421 AAAGAGGCGT CGGGACAAAA TGAACAGTTT CATTGATGAA TTGGCTTCTT TGGTACCAAC
     3481 ATGCAATGCA ATGTCCAGGA AGTTAGATAA ACTCACCGTG CTAAGGATGG CTGTTCAGCA
     3541 CATGAAAACT TTGAGAGGTG CCACCAACCC ATACACAGAA GCAAACTACA AGCCAACATT
     3601 TCTATCAGAT GACGAACTGA AACACCTAAT TCTCAGGGCA GCAGATGGAT TTTTGTTTGT
     3661 CGTAGGATGT GACCGAGGGA AGATCCTCTT TGTCTCCGAG TCTGTCTTCA AGATCCTCAA
     3721 TTATAGCCAG AATGACCTTA TTGGCCAGAG CTTGTTTGAC TACCTGCATC CAAAAGATAT
     3781 TGCCAAAGTT AAGGAACAGC TATCTTCCTC GGACACTGCG CCCCGGGAGC GACTCATTGA
     3841 TGCCAAGACT GGACTTCCGG TTAAAACGGA TATAACCCCT GGGCCCTCCC GGCTATGCTC
     3901 TGGAGCCCGC CGCTCTTTCT TCTGTAGAAT GAAGTGCAAC AGGCCATCAG TAAAGGTGGA
     3961 AGATAAGGAC TTCGCCTCTA CCTGTTCAAA GAAAAAAGCA GATCGAAAAA GCTTCTGCAC
     4021 AATCCACAGC ACAGGCTATT TGAAAAGCTG GCCACCCACG AAGATGGGGC TGGACGAAGA
     4081 CAATGAGCCA GACAACGAGG GCTGCAACCT CAGCTGCCTC GTTGCAATCG GGCGCCTGCA
     4141 CTCGCACATG GTTCCACAAC CAGCGAACGG GGAAATACGG GTGAAATCTA TGGAGTACGT
     4201 TTCTCGACAC GCAATAGATG GGAAATTTGT TTTTGTAGAT CAGAGGGCGA CAGCTATTTT
     4261 GGCGTATCTA CCACAGGAAC TTCTAGGTAC ATCATGTTAT GAGTATTTTC ATCAAGACGA
     4321 CATAGGACAC CTCGCAGAAT GTCACAGGCA AGTTTTACAG ACAAGAGAAA AGATCACAAC
     4381 TAATTGCTAT AAGTTTAAGA TCAAAGATGG TTCTTTTATC ACGCTACGAA GTCGATGGTT
     4441 CAGTTTCATG AACCCGTGGA CCAAGGAAGT TGAATACATT GTCTCAACCA ACACTGTTGT
     4501 TTTAGCCAAT GTCCTGGAAG GCGGGGACCC AACCTTCCCG CAGCTAACAG CACCCCCCCA
     4561 CAGCATGGAC AGCATGCTGC CCTCTGGAGA AGGTGGCCCA AAGAGGACTC ATCCCACTGT
     4621 TCCAGGCATT CCAGGGGGAA CCAGAGCCGG AGCAGGAAAA ATAGGTCGAA TGATTGCCGA
     4681 GGAAATCATG GAAATCCACA GGATAAGAGG GTCATCGCCT TCCAGCTGTG GCTCCAGCCC
     4741 GCTGAACATC ACAAGTACGC CTCCCCCTGA TGCCCCTTCT CCAGGAGGCA AGAAGATTCT
     4801 AAATGGAGGG ACTCCAGACA TTCCTTCCAC TGGACTATTA CCAGGGCAGG CTCAGGAGAC
     4861 CCCAGGGTAT CCCTATTCTG ATAGTTCTTC TATTCTTGGT GAGAACCCCC ACATAGGCAT
     4921 CGATATGATA GATAACGACC AAGGATCAAG TAGTCCCAGT AACGATGAGG CAGCAATGGC
     4981 TGTCATCATG AGCCTCTTGG AAGCAGATGC GGGGCTGGGT GGCCCCGTTG ACTTTAGTGA
     5041 CTTGCCATGG CCGCTGTAGc tcgagcggcc gccagcacag tggtcgacga taaaataaaa
     5101 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     5161 gctagcttaa gtaacgccat tttggaaggc atggaaaaat acataactga gaatagagaa
     5221 gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt
     5281 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc
     5341 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc
     5401 ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc
     5461 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt
     5521 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg
     5581 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc
     5641 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
     5701 acccgtcagc gggggtcttt cattctgcat taatgaatcg gccaacgcgc ggggagaggc
     5761 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5821 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5881 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5941 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6001 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6061 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6121 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6181 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6241 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6301 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6361 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     6421 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6481 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6541 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6601 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6661 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     6721 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     6781 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     6841 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     6901 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     6961 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7021 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7081 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7141 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7201 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7261 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7321 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7381 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7441 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7501 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     7561 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     7621 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7681 ccgcgcacat ttc    
//