LOCUS       pNORg11_mScarlet        8320 bp    DNA        circular     03-DEC-2025
DEFINITION  Shuttle vector pMTL85151. clostron.com
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Heap,J.T., Pennington,O.J., Cartman,S.T. and Minton,N.P.
  TITLE     A modular system for Clostridium shuttle plasmids
  JOURNAL   Journal of Microbiological Methods (2009) 78(1) 79-85
   PUBMED   19445976
COMMENT     pNORg-cymR-CuO-Pveg-mVenus from 1 to 8338
COMMENT     pNORg-cymR-CuO-Pveg-mNG from 1 to 8332
COMMENT     pNORg-Pxyl-mNG from 1 to 8788
COMMENT     pNORg-PbgaL-mNG from 1 to 8832
COMMENT     pNORG10 from 1 to 6603
COMMENT     pMTL85151 from 1 to 3729
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     protein_bind    1074..1101
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt
                     operon of Pseudomonas putida (Mullick et al., 2006)"
                     /locus_tag="CuO"
                     /label="CuO"
                     /ApEinfo_label="CuO"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="#00D95A"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(125..736)
                     /locus_tag="CymR_optimized"
                     /label="CymR_optimized"
                     /ApEinfo_label="CymR_optimized"
                     /ApEinfo_fwdcolor="#ff80ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    995..1024
                     /locus_tag="Pveg"
                     /label="Pveg"
                     /ApEinfo_label="Pveg"
                     /ApEinfo_fwdcolor="#ffff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     terminator      9..62
                     /gene="CD0164 terminator"
                     /locus_tag="CD0164 terminator"
                     /label="CD0164 terminator"
                     /ApEinfo_label="CD0164 terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     63..80
                     /gene="M13R"
                     /locus_tag="M13R"
                     /label="M13R"
                     /ApEinfo_label="M13R"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     gene            complement(2289..2489)
                     /old_locus_tag="LCQ72_18520"
                     /locus_tag="LCQ72_RS18520"
                     /label="LCQ72_RS18520"
                     /ApEinfo_label="LCQ72_RS18520"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     gene            complement(2509..2706)
                     /old_locus_tag="LCQ72_18525"
                     /locus_tag="LCQ72_RS18525"
                     /label="LCQ72_RS18525"
                     /ApEinfo_label="LCQ72_RS18525"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     gene            complement(3326..4375)
                     /old_locus_tag="LCQ72_18535"
                     /locus_tag="RepA_LCQ72_RS18535"
                     /label="RepA_LCQ72_RS18535"
                     /ApEinfo_label="RepA_LCQ72_RS18535"
                     /ApEinfo_fwdcolor="#80ffff"
                     /ApEinfo_revcolor="#80ff80"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     gene            complement(2724..3128)
                     /old_locus_tag="LCQ72_18530"
                     /locus_tag="LCQ72_RS18530"
                     /label="LCQ72_RS18530"
                     /ApEinfo_label="LCQ72_RS18530"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             5964..6587
                     /gene="catP"
                     /locus_tag="catP"
                     /label="catP"
                     /ApEinfo_label="catP"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     gene            4765..5262
                     /old_locus_tag="LCQ72_18540"
                     /locus_tag="LCQ72_RS18540"
                     /label="LCQ72_RS18540"
                     /ApEinfo_label="LCQ72_RS18540"
                     /ApEinfo_fwdcolor="#ff797d"
                     /ApEinfo_revcolor="#ff102f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1835..1912
                     /locus_tag="MCS"
                     /label="MCS"
                     /ApEinfo_label="MCS"
                     /ApEinfo_fwdcolor="#b3ffff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      6800..7345
                     /gene="ColE1 RNA II"
                     /locus_tag="ColE1 RNA II"
                     /label="ColE1 RNA II"
                     /ApEinfo_label="ColE1 RNA II"
                     /ApEinfo_fwdcolor="#999999"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             7746..8186
                     /gene="traJ"
                     /locus_tag="traJ"
                     /label="traJ"
                     /ApEinfo_label="traJ"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(1916..1933)
                     /gene="M13F"
                     /locus_tag="M13F"
                     /label="M13F"
                     /ApEinfo_label="M13F"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             98..124
                     /gene="lacZ alpha"
                     /locus_tag="lacZ alpha"
                     /label="lacZ alpha"
                     /ApEinfo_label="lacZ alpha"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     terminator      2080..2121
                     /gene="Cpa fdx terminator"
                     /locus_tag="Cpa fdx terminator"
                     /label="Cpa fdx terminator"
                     /ApEinfo_label="Cpa fdx terminator"
                     /ApEinfo_fwdcolor="#9d1b1c"
                     /ApEinfo_revcolor="#9d1b1c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(103..124)
                     /locus_tag="pMTL84151 inv Rv"
                     /label="pMTL84151 inv Rv"
                     /ApEinfo_label="pMTL84151 inv Rv"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="#00ff80"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1133..1825
                     /locus_tag="mScarlet-I"
                     /label="mScarlet-I"
                     /ApEinfo_label="mScarlet-I"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cctgcaggat aaaaaaattg tagataaatt ttataaaata gttttatcta caattttttt
       61 atcaggaaac agctatgacc gcggccgctg tatccatatg accatgatta cgaattcgag
      121 ctcgttacct tttgaacttt gcatatcgtt ctctagctat ctctaaagtg ctattacgaa
      181 ctctttcaaa cctctcttta tccttttgcc atagtgatct tacagctaat cctcttacac
      241 tattgaagat taaccacagt atatcttcag catcatctct acttaatcct ctacttacta
      301 gaactcctaa ccacatatct tcaactacaa atctgtttct ttctactgtt ctttgaatcc
      361 cttctcttaa cgcaggatct ctatcagcag caacaattaa gtcaagagaa atggaaaagt
      421 catcatcaag gaaaaattct gctgcatcat ctaacatctg ttgtatcaca tcatcttctg
      481 gtttaagctt agctaatctt gctcttgatc tctcagttat ttgctcatat aaccattcaa
      541 aagttgctag taataactcc aatttagtag gaaaatgatg agattgagca cctcttgata
      601 ctccagctgc acctggaaca tcagcaattc taaatcctgc atatcctttt tctctaagaa
      661 cacctaatgc agcagctatt agttttcctt gtgtttccat ggctctttct gcttgtgttc
      721 ttctttttgg tgacatgatc accatttttt ttactaaagc ttgatctgca atttgaataa
      781 taaccactcc tttgtttatc caccgaacta agttggtgtt ttttgaagct tgaattagat
      841 atttaaaagt atcatatcta atattataac taaattttct aaaaaaaaca ttgaaggaac
      901 ggttatttga ttattaatcc ggctttttta ttatttaggc aactgaaacg attccgatcc
      961 tgtattacta ttctattttg tcaaaataat tttattgaca acgtcttatt aacgttgata
     1021 taatttaaat tttatttgac aaaaatgggc tcgtgtagta caataaatgt agtaacaaac
     1081 agacaatctg gtctgtttgt attatgatta actaataagg aggacaaaca tgGTATCTAA
     1141 AGGTGAAGCT GTTATTAAAG AGTTTATGAG ATTTAAGGTA CATATGGAAG GAAGTATGAA
     1201 TGGTCATGAA TTTGAGATTG AAGGAGAAGG TGAAGGTAGA CCATATGAAG GAACACAAAC
     1261 AGCAAAATTA AAGGTGACTA AAGGAGGACC TTTACCATTT TCATGGGATA TTTTAAGTCC
     1321 ACAATTTATG TATGGATCAA GAGCATTCAT AAAACATCCA GCTGATATAC CAGATTACTA
     1381 TAAACAGTCA TTTCCTGAAG GGTTTAAATG GGAAAGAGTT ATGAACTTTG AAGATGGTGG
     1441 AGCTGTTACT GTAACACAAG ATACTAGCTT AGAAGATGGA ACATTGATAT ACAAGGTTAA
     1501 GTTAAGAGGT ACTAATTTTC CTCCTGATGG TCCAGTTATG CAAAAGAAAA CTATGGGATG
     1561 GGAAGCAAGT ACAGAGAGAC TTTATCCTGA AGATGGCGTA TTAAAAGGAG ATATAAAGAT
     1621 GGCTTTAAGA CTAAAAGATG GAGGAAGATA TTTAGCAGAT TTCAAAACTA CCTATAAAGC
     1681 CAAAAAACCA GTACAAATGC CTGGAGCTTA TAATGTTGAC AGAAAACTTG ACATAACTTC
     1741 TCACAATGAG GATTATACAG TTGTAGAACA ATATGAGAGA TCAGAAGGAA GGCATTCTAC
     1801 TGGAGGGATG GATGAACTTT ATAAGTAAga attcgagctc ggtacccggg gatcctctag
     1861 agtcgacgtc acgcgtccat ggagatctcg aggcctgcag acatgcaagc ttggcactgg
     1921 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     1981 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     2041 cccaacagtt gcgcagcctg aatggcgaat ggcgctagca taaaaataag aagcctgcat
     2101 ttgcaggctt cttattttta tggcgcgcca ccaaagaata gcaccaaact gataaaccaa
     2161 ccttagatat ctatttaatt gacaaattaa aggtggcaaa gtatatgagg cagcaactac
     2221 ctacctcata tacttttgcc ttttaattcc ctcatctgtt ctttcatcca tttatttcac
     2281 tgacttttct ataaaaccca agattgctga atatgcttgt acaatccatt ctgatcaagc
     2341 agatcattcg ttacctcaat atcgtaagat ggctcttcct gttggaaaaa cgttccttgc
     2401 gcatccacta taacaatcgt tccatttttt attttttgtc cataccgaaa taatacaata
     2461 tctccttttt tatacttcac ctcatccatg cgtttcctcc tttctatttc atactacctg
     2521 aacttccact attttgtttc tatacagaac tttcatttga tttccttttt tatcttttac
     2581 aattgcttct tcatctgata caaaatatac atctacactt tcttcttcaa aatttttgtt
     2641 aatctcttgc attattttgt attggctaac tgtatgcaac ccttttatag tataactatt
     2701 tttcattatc attttccttt cttttatttc caaaacttcc accatttctt atttgattct
     2761 tcttgatttt ctatcaacat tttttgctct gttaccatgt ggatctgttg ttcctgatct
     2821 aacagcttct ggcattccat aagtcttttg cttaattcat ctatctgttt atttttatgc
     2881 tctagctcat tacggagcag atcaacaata gcttgattca ctacatcatc tgctttcgct
     2941 aattgcgcaa catctttcgc atctttcgca acatcaacct tataatgctt ataaatccta
     3001 tattctatag tttcgctaat ttcgaaactt cctttcgcat ctttcgcgat atgatttttc
     3061 ctacaccatg ctctaacagc ttgttcagat actccgcatg tctcagcaat ttctcttgtt
     3121 gttttcatgt tttctcctta taccactagt tataaaccat atatacgaaa ctgtcatatt
     3181 taaaatcgag agttctgttc gtattttact gtatttctat caaaattaaa tttgatagtg
     3241 ttttgcgctg gtataatcat actctcacaa tcactgcgct ttcatatact ctgaaagaat
     3301 atatctataa gttttccgat ttttgttatg cattattttc aactaaatac ttcatatatc
     3361 caaaccggct cttcacatct tttttgttct gcagagattt atattttcgt aacaaatagt
     3421 gatagatgtc cagatcatga cgtcctgcat ctgttggctc atactctaca tgttcctctg
     3481 caagcagtgc cagttcttcc aactgtgatc gattaaattc attgccacat gcttcagacc
     3541 ataacgtaaa tttactttca agaaccgcat tttgttcctg atctgcagtc gaatcagagt
     3601 ctggacaata ttccggaaaa tcgtatattt ccatctgccc atgtatttgc tcttgttctg
     3661 cagtacactt ttttatttta aatttaattg cccgcgttaa acgccctttg gttatttttt
     3721 cgtattcaaa ttcaatatct gtaacttcat ttatgtcttt cttacatttt tgtagcactt
     3781 tattaaattc tcgaaagctc tcattatttt tatttgttgc ccccattagt tctcttaaat
     3841 ctttaagctc tacagtccat tctctcctgt ataatttgtc ctttaaaaga ggatataatt
     3901 ttagagaata ttttgatttc aaacgcaaag aatagtctaa tgtatattca atatatcctt
     3961 tttcagccaa tccaaaaaat gcttctttga gttttggatt acagtcgatc gttattgttc
     4021 tttttccgaa atctggatca ctcacaataa ctcttgcgtc cgaaaataaa tttactttta
     4081 tataatcccc attattattc aaatcaattt ttattcttga accaagaagt ttgtcaagac
     4141 tcttttcaat ttcgctgctt ttcaatttat tgggatactc atatccaagt aaccttaaat
     4201 attcatcaat agtaaagact acttccgaac tttcttcttc tcttgcattt attttagcca
     4261 agtatgtatc aatcaatctc atttctccaa ggttgtattc tgaaaacttt gaccatatta
     4321 aaggtttcga tttttcgact atataatttt tagttaatgt atttttttta gacatgagcc
     4381 acctcctatt ccctattata atataatagt ggtatattgt caatctttat ataccactat
     4441 atgccgcaaa aaataccact atataccgca aaaaatacca ccgttatacc gcaaaaaata
     4501 ccactatata ccgcaaaaaa taccactata tgccgcaaaa aataccacca ttataccgca
     4561 aaaacgttga ttttacgctg tttattaact ccgtaaacaa gtaattaaac aagtaattaa
     4621 ataaaatatt aaacaagcta actaacacgc gcgtacgcgc aaggttagtt ggcttgctag
     4681 ttttttcaat caaaattaaa tttgaccgta gcggtgaatt atgctataat tcaatcaaaa
     4741 ttaaatgtga aagtagtgaa tagcatgaac gaaaaatcaa tgcaaaagat aaaagaatat
     4801 gcaaaagagc gtaaggatct atatctgcag tatagtgttt cggaaaaaaa tattccggaa
     4861 agtatcaaaa aacagaacaa agaaaatctg aaactgatgc aagatgcact ggcaacatta
     4921 ggcgttcgat tgaatatcaa agatggagag atctcgcttc tgatgcacac atcaaatttt
     4981 gtagatcgta aaacaaggag agctggccgg aaaagaacat atgcgttaaa ggagcaagaa
     5041 caaggaaact acacagcaga tgcataccgg ttttcagatg tgatattgct gattgaagaa
     5101 aaaggggata aggagacgca aatcattctt ggaatgagtg aatcgactta tttccggcat
     5161 aaaaagaaaa tgaaagccag tgaatactac agtttgttag acccacaaaa aatgacggat
     5221 cggatgtatc tggaatccgt aaaaggcaac aactactttt agactttcaa ttttaatttc
     5281 ggtagtacta ttgacgacag aaataagcta ttgtaaaata actgcagaag taaggtggcc
     5341 tcgtcgcaat tggagcggcg ctagcgcaaa cttttaggac aggaataaga taacccacag
     5401 caattggagc ggtgcaagtg tcaattcctg tccgtttttt attatctgac aattacagac
     5461 tgcagaacga aaattgtgtt tgaaaaagag acaattaggg gatacttgaa aagcttatgg
     5521 tcatatgcta taatgcctat aggcaaaacg atagaaagtg ctcatatggc acaacaaaac
     5581 ccccacgagt tcgcacctcg tgggggtttt tatgccgttt tgggtgggtg gcttaaaccc
     5641 caaggttgct accagtcatt cttgtccaac catttgcaga tgtagtaggc aactacactc
     5701 gccaggacag aaagtataaa cgatagaaat tcactcatat ggcacacccc ctttctctac
     5761 ctgtctaggg gtggtagctt atccagtata gcatatgaca tttaatactg tcaattttaa
     5821 aactgacagt attagaggag aaatcaacaa aatacgtatg tttgtgtgtg gccggccagt
     5881 gggcaagttg aaaaattcac aaaaatgtgg tataatatct ttgttcatta gagcgataaa
     5941 cttgaatttg agagggaact tagatggtat ttgaaaaaat tgataaaaat agttggaaca
     6001 gaaaagagta ttttgaccac tactttgcaa gtgtaccttg tacctacagc atgaccgtta
     6061 aagtggatat cacacaaata aaggaaaagg gaatgaaact atatcctgca atgctttatt
     6121 atattgcaat gattgtaaac cgccattcag agtttaggac ggcaatcaat caagatggtg
     6181 aattggggat atatgatgag atgataccaa gctatacaat atttcacaat gatactgaaa
     6241 cattttccag cctttggact gagtgtaagt ctgactttaa atcattttta gcagattatg
     6301 aaagtgatac gcaacggtat ggaaacaatc atagaatgga aggaaagcca aatgctccgg
     6361 aaaacatttt taatgtatct atgataccgt ggtcaacctt cgatggcttt aatctgaatt
     6421 tgcagaaagg atatgattat ttgattccta tttttactat ggggaaatat tataaagaag
     6481 ataacaaaat tatacttcct ttggcaattc aagttcatca cgcagtatgt gacggatttc
     6541 acatttgccg ttttgtaaac gaattgcagg aattgataaa tagttaactt caggtttgtc
     6601 tgtaactaaa aacaagtatt taagcaaaaa catcgtagaa atacggtgtt ttttgttacc
     6661 ctaagtttaa actccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6721 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6781 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6841 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6901 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6961 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7021 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7081 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7141 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7201 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7261 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7321 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7381 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7441 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7501 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcagg gccccctgct
     7561 tcggggtcat tatagcgatt ttttcggtat atccatcctt tttcgcacga tatacaggat
     7621 tttgccaaag ggttcgtgta gactttcctt ggtgtatcca acggcgtcag ccgggcagga
     7681 taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc
     7741 tggcggtgct caacgggaat cctgctctgc gaggctggcc ggctaccgcc ggcgtaacag
     7801 atgagggcaa gcggatggct gatgaaacca agccaaccag gaagggcagc ccacctatca
     7861 aggtgtactg ccttccagac gaacgaagag cgattgagga aaaggcggcg gcggccggca
     7921 tgagcctgtc ggcctacctg ctggccgtcg gccagggcta caaaatcacg ggcgtcgtgg
     7981 actatgagca cgtccgcgag ctggcccgca tcaatggcga cctgggccgc ctgggcggcc
     8041 tgctgaaact ctggctcacc gacgacccgc gcacggcgcg gttcggtgat gccacgatcc
     8101 tcgccctgct ggcgaagatc gaagagaagc aggacgagct tggcaaggtc atgatgggcg
     8161 tggtccgccc gagggcagag ccatgacttt tttagccgct aaaacggccg gggggtgcgc
     8221 gtgattgcca agcacgtccc catgcgctcc atcaagaaga gcgacttcgc ggagctggtg
     8281 aagtacatca ccgacgagca aggcaagacc gatcgggccc  
//
