LOCUS       pAK-Fi-368_T7-ZEBOVmg-Z 5391 bp ds-DNA     circular     21-OCT-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..431
                     /label="genscript pUC57 EcoRV (1-431)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          379..395
                     /label="M13-F"
                     /note="sequence: GTAAAACGACGGCCAGT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    396..431
                     /label="pUC57 MCS"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    429..431
                     /label="pUC57 EcoRV 5'end"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    432..3106
                     /label="ZEBOV wt minigenome part 3"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        486..503
                     /label="t7 promoter sequence"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    503..1233
                     /label="ZEBOV 5' trailer (731nt)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(1179..1189)
                     /label="Lpol poly(A) signal"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             complement(1240..2361)
                     /label="Translation 2361-1240"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREFALRDPAGNCVHFVAEEQDGSGSGSGSGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"
     gene            complement(1243..1959)
                     /label="eGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="note: /created by=andreas.puschnik"
     misc_feature    complement(1960..1989)
                     /label="GS linker"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             complement(1990..2361)
                     /label="ZeocinR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 label: ZeocinR transl_table: 1 translation: MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREFALRDPAGNCVHFVAEEQD"
     misc_feature    2377..2848
                     /label="ZEBOV 3' leader (472nt)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(2849..2934)
                     /label="Hepatitis delta ribozyme in this sequence"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3113..5391
                     /label="genscript pUC57 EcoRV (432-2710)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3113..3115
                     /label="pUC57 EcoRV 3'end"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3113..3157
                     /label="pUC57 MCS"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(3606..4171)
                     /label="pUC57 rep (pMB1)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(4332..5191)
                     /label="pUC57 bla (amp-r)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tGGTCTCATA TGCTCGAGGG TACCCCCGGG GAGCTCTTAA TTAATCGATG
      481 CTAGCTAATA CGACTCACTA TAGGACACAC AAAAAAGAAG AAATAGATTT ATTTTTAAAT
      541 TTTTGTGTGC GACCATTTTT CCAGGAATCC TTTTTGCAAC GTTTATTCTG GCGAGCCTTC
      601 TGATGAGCGT GGTCAATGTC TTAATTATCA TTAACACGAA GATTATTATC TAGTCTGCTA
      661 TATTGTAAAG AAGGTATTTC TGATTTTACA GTCCTGCCTT TTCTTTTAAT TTTATCATTC
      721 TGTATGAAAT AGTAGCTTAC TAGACAAAAA TGTTAAGGTT ATTACACCTA ATCTCTTATT
      781 TTACACAAAG ATTAAGGCTA TCACCGTTTT TAAAACGACA AATTAAAGTA ATGATAATTA
      841 TAAAAAACTT AATCTTAAAG ATCAATTTTG TTTTAAGAAG TTGTGTTATC AACCAAAACA
      901 CTATTCCATC TGACAGGATA TTGATACAAC ATTATTATAT CAATAACAAT ATGAGCCCAG
      961 ACCTTTCGTT AAAGCTTAAT TATAAAGAGT GCAGAGTTTA TTATGTTGCG TTAAATTCAT
     1021 TGCGGCAGTA ATATGTCACA CATGCTGCAT TGTGAATTTT TTGATTATCA CGCAGGGAGA
     1081 GAGGCTAAAT ATAGTATATA ATATAGGGAC TCCTTTCGTT ATTTAAATAG TTGTTTATTG
     1141 AGATTAGATC AATATGATGT ATGAGAGCAA TTTATGAGTT TTTTATAATC TGTATGTTAA
     1201 TAACCAACCT TTGCAAGTAT CAGGATACTA TGCGGCCGCt caCTTGTACA GCTCGTCCAT
     1261 GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC
     1321 GTTGGGGTCT TTGCTCAGGG CGGACTGGGT GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC
     1381 GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC
     1441 CTCGATGTTG TGGCGGATCT TGAAGTTCAC CTTGATGCCG TTCTTCTGCT TGTCGGCCAT
     1501 GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC
     1561 CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC CCTCGAACTT
     1621 CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC GCTCCTGGAC
     1681 GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT CGGGGTAGCG
     1741 GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG GTGGGCCAGG GCACGGGCAG
     1801 CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT CGCCCTCGCC
     1861 CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA CCAGGATGGG
     1921 CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG CTGCCGCTAC CGCTACCGCT
     1981 GCCGCTACCa tcttgttctt cagcaacaaa atgaacacaa tttccagctg gatctctcaa
     2041 agcaaattct ctaccccatg gctgttcacc aatctcggtc atagctggac cagaagcatc
     2101 tctgaagttt gtagatacaa cttctgacca ttcagcatat aattcatcta aacccctgac
     2161 ccagacccaa gccaatgtat tatcagggac aacttgatct tggactgctg agataaataa
     2221 agtaacgtca tccctgacaa caccagcaaa atcgtcctca acaaagtctc ttgagaatcc
     2281 taacctatct gtccaaaact ctacagcacc tgcaacatcc ctagctgtta aaacaggaac
     2341 ggcagatgtt aatttagcca tCATACGCGT ATGAGACATA CTCGGAATTT TGTGATTCCG
     2401 AGCAATTTGT CTTTTAACTA TTTCCAATGT TTTACCCCAA GCTTTAGGGT TGTTGAATCT
     2461 TCAATCCCCT AAGTGTTCAA GTTCAAAACC AAGGTTAATT CTCAATCTTG CTTGTTTGAC
     2521 TGTGAACTAA TGCTGTCCTT TAACAATAAT GTTTCTATTA AGGTTGTTGG ATCACCCTGC
     2581 AGAGTCTGAG GTGGAACTTG TGATGTGATA AGACCTAGGT GTTGACAAGG GATTTTCAAC
     2641 TGAGCACACT GATGCCCTTG CTCCCTTCGG AGACCTAGGT TGTTCTCTAT CTTTGTGATG
     2701 TGGCTCTGAA ACAAACCAGG TGTGATTACA GTAACAATTT CAATTTAAAT TCCGATATAA
     2761 ATTTCAATGA GAGGAAAATT ATTAATCTTC CTCATAGTTA TTCGCACACA AAAGATCCTA
     2821 AAAATTCTTC TTTCTTTTTG TGTGTCCGGG GTCGGCATGG CATCTCCACC TCCTCGCGGT
     2881 CCGACCTGGG CATCCGAAGG AGGACGTCGT CCACTCGGAT GGCTAAGGGA GAGCTCCCTA
     2941 GATCGGAACT CGCTAGCAGA TCTTTTTCCC TCTGCCAAAA ATTATGGGGA CATCATGAAG
     3001 CCCCTTGAGC ATCTGACTTC TGGCTAATAA AGGAAATTTA TTTTCATTGC AATAGTGTGT
     3061 TGGAATTTTT TGTGTCTCTC ACTCGGAAGG ACATATGGGG GATCCTGAGA CCatcggatc
     3121 ccgggcccgt cgactgcaga ggcctgcatg caagcttggc gtaatcatgg tcatagctgt
     3181 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     3241 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     3301 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     3361 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     3421 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3481 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     3541 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     3601 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3661 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3721 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     3781 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3841 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3901 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3961 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4021 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4081 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4141 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4201 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4261 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4321 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4381 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4441 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4501 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4561 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4621 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     4681 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     4741 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4801 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4861 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4921 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4981 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5041 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5101 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5161 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5221 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5281 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     5341 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt c
//