LOCUS       pABDP1                  9381 bp    DNA     circular SYN 09-NOV-2025
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 10, 2025 from SnapGene 8.0.2 to Vector NTI(R) format
            https://www.snapgene.com
COMMENT     Sequence Label: pABDP1
COMMENT     VNTDATE|1010880000|
COMMENT     VNTDBDATE|1109462400|
COMMENT     VNTNAME|pABDP1|
FEATURES             Location/Qualifiers
     primer_bind     complement(9..26)
                     /locus_tag="M13-fwd"
                     /label=M13-fwd
     CDS             97..165
                     /locus_tag="LacZ alpha"
                     /label=LacZ alpha
     CDS             802..1461
                     /locus_tag="AmpR"
                     /label=AmpR
     rep_origin      1559..2241
                     /locus_tag="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    2400..3199
                     /label=psbH downstream flanking element
     misc_feature    complement(2417..2550)
                     /label=trnE2
     primer          3138..3158
                     /label=Bac Col pcr FW
                     /note="sequence: cgtcaggcaatttgcttacac"
     primer          3160..3219
                     /label=pASapl_psbA3 Fw
                     /note="sequence: 
                     tttaaattaaaaattaaatttaaagaaaagtgagctattaAAACTTAGCATGTTTACAT
                     A"
     primer          complement(3180..3239)
                     /label=pASapI-psbA rv
                     /note="sequence: 
                     TAAGACGTCCTCCTTCGGAGTATGTAAACATGCTAAGTTTtaatagctcacttttcttt
                     a"
     terminator      complement(3200..3602)
                     /label=psbA/ 3' UTR
     primer          3563..3622
                     /label=PsbA-mVenus  FW
                     /note="sequence: 
                     AGGTATGGTTAACCAGATTTATTTTAGTTTAAAAAAAAATttaTTTATATAATTCATCC
                     A"
     primer          complement(3583..3642)
                     /label=psbA 3UTR_mVenusRv
                     /note="sequence: 
                     TGCTGGTATTACATTAGGTATGGATGAATTATATAAAtaaATTTTTTTTTAAACTAAAA
                     T"
     CDS             complement(3603..4322)
                     /codon_start=1
                     /label=mVenus
     primer          3603..3622
                     /label=tdTmato-rbcLRV
                     /note="sequence: 
                     tcacgagcaaggtcacgaccttcgttacgagcttgagtacttaTTTATATAATTCATCC
                     A"
     primer          4283..4342
                     /label=mVenus atpA fw
                     /note="sequence: 
                     GAACTACACCAGTGAATAATTCTTCACCTTTTGAAACCATTTTATATAAATAAATGTAA
                     C"
     primer          complement(4303..4362)
                     /label=mVenus-atpA Rv
                     /note="sequence: 
                     TTTTAGGATCGTCAAAAGAAGTTACATTTATTTATATAAAATGGTTTCAAAAGGTGAAG
                     A"
     primer          complement(4303..4323)
                     /label=atpA-tdTomat FW
                     /note="sequence: 
                     AATAAACTTTTTTAATCTTTTATTTATTTTTTCTTTTTTAATGGTATCTAAAGGTGAAG
                     A"
     promoter        4323..5178
                     /label=BDP1
     primer          5139..5198
                     /label=atpA-tdTomat FW
                     /note="sequence: 
                     AATAAACTTTTTTAATCTTTTATTTATTTTTTCTTTTTTAATGGTATCTAAAGGTGAAG
                     A"
     primer          complement(5159..5218)
                     /label=atpA-Td tomato RV
                     /note="sequence: 
                     GCATGAATTCCTTAATTACTTCTTCACCTTTAGATACCATTAAAAAAGAAAAAATAAAT
                     A"
     CDS             5179..6609
                     /codon_start=1
                     /label=td Tomato
     primer          complement(5929..5944)
                     /label=atpA-Td tomato RV
                     /note="sequence: 
                     GCATGAATTCCTTAATTACTTCTTCACCTTTAGATACCATTAAAAAAGAAAAAATAAAT
                     A"
     primer          6570..6629
                     /label=Td  tomato-rbcL fw
                     /note="sequence: 
                     TCACTTATTTTTATATGGTATGGATGAATTATATAAAtaagtactcaagctcgtaacga
                     a"
     primer          complement(6590..6649)
                     /label=tdTmato-rbcLRV
                     /note="sequence: 
                     tcacgagcaaggtcacgaccttcgttacgagcttgagtacttaTTTATATAATTCATCC
                     A"
     primer          complement(6590..6609)
                     /label=PsbA-mVenus  FW
                     /note="sequence: 
                     AGGTATGGTTAACCAGATTTATTTTAGTTTAAAAAAAAATttaTTTATATAATTCATCC
                     A"
     terminator      6610..7022
                     /locus_tag="rbcL 3' UTR element "
                     
                     
     primer          complement(6615..6634)
                     /label=Bac Col pcr rv
                     /note="sequence: cgaccttcgttacgagcttg"
     misc_feature    7023..9381
                     /locus_tag="psbH upstream flanking element"
                     /label=psbH upstream flanking element
     CDS             complement(7168..7434)
                     /codon_start=1
                     /locus_tag="psbH"
                     /label=psbH
     CDS             8004..8138
                     /codon_start=1
                     /label=psbN
ORIGIN
        1 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
       61 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      121 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      181 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      241 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
      301 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
      361 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
      421 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      481 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      541 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      601 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      661 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      721 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      781 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      841 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
      901 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
      961 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1021 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1081 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1141 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1201 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1261 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1321 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1381 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1441 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1501 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1561 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1621 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1681 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1741 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     1801 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1861 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1921 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     1981 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2041 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2101 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2161 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2221 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2281 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2341 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagca
     2401 attcgaatcc gcgttttctc cgtgaaaggg aggtgtccta ggcctctaga cgatgggggc
     2461 tttttgttat attttactaa atatatatta taattaaaaa aaattgaatt gtcaattttt
     2521 aatgtacact tagttgaaag tgcccctgtc cccttggcca tatttaacag aagttattta
     2581 taacgcagct gttttttgga gtctataaat ttataacatc agttactatg gatttccctt
     2641 tagttttatg gcctaggacg tccccttccc cttcgatgct ggaggcatcc ttttacggga
     2701 caataaataa atttgttgcc tcgcctatcg gctaacaagt tccttcggag tatataaata
     2761 taggatgtta atactgctat aaactttagt tgcccaatat ttatattagg acgccagtgg
     2821 cagtggtacc gccactgcct gcttcgcagt atataaatat aggcagttgg caggcaactg
     2881 ccactgacgt cctattttaa tactcccaag tttacttgcc taggcagttg gcaggcaaca
     2941 aatttattta ttgtccacta aaatttattt gcccgaaggg gacgtccact aaaatttatt
     3001 tacccgaagg ggacgtccta atataaatat ggggatgtca atgctccgtt aggaagtaac
     3061 taacgttttt caaataaatt ttatcccgga gggaagtagg cagtagcccg ccactgtcat
     3121 cctttaagtg gatctctcgt caggcaattt gcttacacct ttaaattaaa aattaaattt
     3181 aaagaaaagt gagctattaa aacttagcat gtttacatac tccgaaggag gacgtcttac
     3241 ggaatataaa tattagtcga gtggtaccgc cactgcctag tatataaata tcggcagttg
     3301 gcaggatatt tatatactcc gaaggaactt gttagccgat aggcgaggca actgccacta
     3361 aaatttattt gcctcctaac ggagcattaa aatccctaag tttacttgcc cgtaagggga
     3421 aggggacgtc cactaatatt tatattaggc agttggcagg caacaataaa tacatttgtc
     3481 ccgtaagggg acgtcctgcc aactgcctat ggtagctatt aagtatatat atatgaaaag
     3541 tgtgtataaa ctaaaataaa ccaggtatgg ttaaccagat ttattttagt ttaaaaaaaa
     3601 atttatttat ataattcatc catacctaat gtaataccag cagctgttac aaattctaat
     3661 aaaaccatat gatcacgttt ttcatttgga tcttttgata atttagattg ataactaaga
     3721 taatgattat caggtaataa aacaggacca tctccaattg gtgtattttg ttgataatga
     3781 tcagctaatt gaacaccacc atcttcaata ttatgacgaa ttttaaaatt agctttgata
     3841 ccatttttct gtttatcagc tgtaatataa acattatgtg aattataatt atattctaat
     3901 ttatgaccta aaatattacc atcttcttta aaatcaatac ctttaagttc aatacgatta
     3961 actaatgtat ctccttcaaa tttaacttca gcacgtgttt tataattacc atcatcttta
     4021 aagaaaattg tacgttcttg tacataacct tcaggcattg cacttttaaa gaaatcatgt
     4081 tgtttcatgt gatctggata acgagcaaaa cattgtaaac cataacctaa tgtagtaact
     4141 aaagttggcc atggaactgg taatttacct gttgtacaaa ttaattttaa agttaattta
     4201 ccataagttg cgtcaccttc accttcacca ctaactgaaa atttgtgacc attaacgtca
     4261 ccatctaatt caactaaaat tggaactaca ccagtgaata attcttcacc ttttgaaacc
     4321 attttatata aataaatgta acttcttttg acgatcctaa aataatctgt ccggaaatat
     4381 aatttaaaaa ttgttgaaaa attttaaata catttaaata tattatatat ttagaaataa
     4441 aaaataatgt aaactagcaa atttatgtac ttgtcttatc agtcgagcat agaaagggcc
     4501 tttaggtata tgtcggtcgt cttacgcagt ttacttacta aaaccatgta tattcctgtc
     4561 ggggataagc gaggccactg ttcactcctc tccaatatag tagactttat tagaggcagt
     4621 gtttatatac ctaaacgtca aaagtcattt ttataactgg tctcaaaata cctataaacc
     4681 cattgttctt ctcttttagc tctaagaaca atcaatttat aaatatattt attattatgc
     4741 tataatataa atactatata aatacattta cctttttata aatacattta cctttttttt
     4801 aatttgcatg attttaatgc ttatgctatc ttttttattt agtccataaa acctttaaag
     4861 gaccttttct tatgggatat ttatattttc ctaacaaagc aatcggcgtc ataaacttta
     4921 gttgcttacg acgcctgtgg acgtcccccc cttcccctta cgggcaagta aacttaggga
     4981 ttttaatgca ataaataaat ttgtcctctt cgggcaaatg aattttagta tttaaatatg
     5041 acaagggtga accattactt ttgttaacaa gtgatcttac cactcactat ttttgttgaa
     5101 ttttaaactt atttaaaatt ctcgagaaag attttaaaaa taaacttttt taatctttta
     5161 tttatttttt cttttttaat ggtatctaaa ggtgaagaag taattaagga attcatgcgt
     5221 tttaaagttc gtatggaagg tagtatgaat ggtcatgaat ttgaaattga aggtgagggt
     5281 gaaggtcgtc catatgaagg tacacaaact gctaagttaa aagtaactaa aggtggtcca
     5341 ttaccatttg catgggatat tttatcacca caattcatgt atggttcaaa agcttatgta
     5401 aaacacccag ctgatattcc tgactataaa aaattatcat ttccagaagg ttttaaatgg
     5461 gaacgtgtta tgaactttga agatggtggt ttagtaacag taactcaaga ttctagttta
     5521 caagatggta cattaatata taaagttaaa atgcgtggta ctaattttcc acctgatggt
     5581 ccagtaatgc agaaaaagac aatgggttgg gaagcttcaa ctgaacgttt atatccacgt
     5641 gatggtgtac taaaaggtga aattcatcaa gcacttaaac ttaaagatgg tggtcattac
     5701 ttagtagaat tcaaaacaat ctatatggct aaaaaacctg tacaattacc aggttattat
     5761 tatgttgata ctaaattaga tattactagt cataatgaag attatacaat tgtagaacaa
     5821 tatgaacgtt cagaaggtcg tcatcatctg tttttaggtc atggtactgg atctacaggt
     5881 tcaggtagta gtggtacagc ttcttctgaa gataataata tggctgttat taaggaattc
     5941 atgcgtttta aagtaagaat ggaaggtagt atgaatggtc atgaatttga aattgaaggt
     6001 gaaggtgaag gtagaccata tgaaggtaca caaactgcta agttaaaagt tacaaaaggt
     6061 ggtccattac cttttgcttg ggatatttta agtccacaat tcatgtatgg ttctaaagct
     6121 tatgttaaac atccagctga tattccagat tataaaaaat taagttttcc tgaaggtttt
     6181 aaatgggaac gtgtaatgaa ttttgaagat ggtggtttag ttacagttac acaagacagt
     6241 tctttacaag atggtacttt aatctataaa gttaaaatga gaggtactaa ttttccacct
     6301 gatggtccag taatgcaaaa gaaaacaatg ggttgggaag caagtacaga acgtttatat
     6361 cctcgtgatg gtgttttaaa aggtgaaatt caccaagctt taaagttaaa agatggtggt
     6421 cactacttag tagagtttaa aacaatatat atggctaaaa aacctgtaca attaccaggt
     6481 tactattatg ttgatactaa attagatatt acatcacata atgaagatta tacaattgtt
     6541 gaacaatatg aacgtagtga aggtcgtcat cacttatttt tatatggtat ggatgaatta
     6601 tataaataag tactcaagct cgtaacgaag gtcgtgacct tgctcgtgaa ggtggcgacg
     6661 taattcgttc agcttgtaaa tggtctccag aacttgctgc tgcatgtgaa gtttggaaag
     6721 aaattaaatt cgaatttgat actattgaca aactttaatt tttatttttc atgatgttta
     6781 tgtgaatagc ataaacatcg tttttatttt tatggtgttt aggttaaata cctaaacatc
     6841 attttacatt tttaaaatta agttctaaag ttatcttttg tttaaatttg cctgtcttta
     6901 taaattacga tgtgccagaa aaataaaatc ttagcttttt attatagaat ttatctttat
     6961 gtattatatt ttataagtta taataaaaga aatagtaaca tactaaagcg gatgtagcgc
     7021 gtttatctta acggaaggcc agtggcagtg gcggtgccac tgccgaatat aaatatggtt
     7081 gagttgctta gtttacctta gcgaaaagaa gacttagcag ctagccttaa caaacagttt
     7141 tatattttat gtttgtgtta aataaaatta agaaacttta gctaaagttt cccaactcat
     7201 agaaacgtca tctaaaatta aagaactgtt gtaaatttct aaaatgatta ataagaatgc
     7261 tgcaaataaa aggataaata cagccattaa aacagttgta ccccagcctg gtaatacttt
     7321 acctgcttct gagttaagtg gacgtaataa agtacctaat ggtgtaacta aaccaggttc
     7381 ttggaagtct gaatttactt ttgatggttt agctttagaa gttcctgttg ccataattga
     7441 ttaaatgaat taagcgttat tagcgctatt ttatttactt tctgtaaaaa ataaggaaaa
     7501 tattcttcag tgcattccct ctcaggatta taaatactct gaggataacg ttctctcgtc
     7561 aaggggttgc ttcttgtgag tatagaaacc tactagcaca agaaataaat tgcataaaaa
     7621 tgtatttacc taggaccgca gtaggcagtc ccttttcccc ttcagaactg cctgctttaa
     7681 aagaatgaaa aaactgcctt gtctggtaag taaaactctt taattactca ctaaagacga
     7741 tcttagaagt tctttgttca ttttttattt aatataatat ttgttatata aaaattaaat
     7801 aatttttaat taatgtttaa ctttgtaagg acagtttcaa agtgacatga atggctactg
     7861 caaaaacgaa gtaagttatt ctttctcagg gcaaaatttt gagtagatta attttgttta
     7921 aaaatgtggg acacagtcgt caagtctttt gaactatcta agagatatgt tgaaaagaga
     7981 ataattttat tattaaatga gctatggaaa gtccagcttt tttctttacc ttttttttat
     8041 ggtttcttct gttaagtgta actggctatt cagtttatgt tagttttggt ccaccttcaa
     8101 aaaaattacg tgatcctttt gaagaacacg aagattaaac aagttaaaaa gtactatttt
     8161 tacaagtgac ttcggtgcct ctgagaaccc tagttatagt gatataaaat aactagctaa
     8221 ctactttata tttttatgaa agtcattttg tcgagcatat aaacaaaaac aaaattgcta
     8281 tactaggcag tcacagtgca actgtctccg tctccttaac cgagaaaggg taaacgtctt
     8341 cggtaaagta acaaacttta gttatgttaa ctgcttgcga gttaaccatt ttttttcctc
     8401 cgaaggacaa cagttggcag ttgccaaact ttagtggtct aatatttata ttaggcagtt
     8461 ggcaggcaac tgcactgacg tcccgaaggg gaaggggttt acttacctcc taacggagta
     8521 tataaataga ataaaattta tttcctgcgc tagcagattt acatactagg attttaatac
     8581 tccgaaggag gcagtggcgg taccactgcc actggcgtcc tccttcccct tcgggcaaat
     8641 gcattttagt gccacttaag tttacttgcc taggcagttg gcaggacgtc agtggcagtg
     8701 gtaccgcgac tgcctatatt tatatactcc taagtttact tgcctaggca gttggcaggc
     8761 aactgccact gacgtccttc cccttcccct tcgggacgtc cccttacggg aatataaata
     8821 ttagtggata tttatatact gcgatgttta catactccga aggaggagag ctagcagttg
     8881 cctgccaact gcctaatata aatattgggc aagtaaactt agaatgttta catactccga
     8941 aggaggacgt cccttacggg aatataaata ttagtggcag tggtaccgcc actgcctcct
     9001 tcggagtatt aaaatcctag tatataatat accgtaaggg acgtcctccg acggtggcag
     9061 tggcggtacc actgccaccg gcgtcctaat atacatattg aagtatttaa acctgttagc
     9121 gcacgctcta acgagtcagt aaacttccct tttggggctt ctaggcagcg cataaatttt
     9181 ctaggacgaa cgtccactgg cgtctcgtaa ggagcagtga caggccacta atgtcccctt
     9241 aatgggtaaa taaatggcta tcgtctatcc atgaagagac catatattcc agtagcaccg
     9301 ttatgatcct caaagggtaa caccatttgt atagtattat ggtgaaatgc atccctttca
     9361 gggtagattt atatcttaca g
//
